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On Fiber Derivatization With Solid Phase Microextraction Coupled To Capillary Electrophoresis With Laser Induced Fluorescence Detection For Chemical Analysis Of The Content Of Small Objects
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Book Synopsis On-fiber Derivatization with Solid Phase Microextraction Coupled to Capillary Electrophoresis with Laser Induced Fluorescence Detection for Chemical Analysis of the Content of Small Objects by : Angelina So
Download or read book On-fiber Derivatization with Solid Phase Microextraction Coupled to Capillary Electrophoresis with Laser Induced Fluorescence Detection for Chemical Analysis of the Content of Small Objects written by Angelina So and published by . This book was released on 2001 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Solid Phase Microextraction Coupled to Capillary Electrophoresis with Axially Illuminated Laser Induced Fluorescence Imaging Detection by : Tiemin Huang
Download or read book Solid Phase Microextraction Coupled to Capillary Electrophoresis with Axially Illuminated Laser Induced Fluorescence Imaging Detection written by Tiemin Huang and published by . This book was released on 2000 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Handbook of Solid Phase Microextraction by : Janusz Pawliszyn
Download or read book Handbook of Solid Phase Microextraction written by Janusz Pawliszyn and published by Elsevier. This book was released on 2011-12-01 with total page 497 pages. Available in PDF, EPUB and Kindle. Book excerpt: The relatively new technique of solid phase microextraction (SPME) is an important tool to prepare samples both in the lab and on-site. SPME is a "green" technology because it eliminates organic solvents from analytical laboratory and can be used in environmental, food and fragrance, and forensic and drug analysis. This handbook offers a thorough background of the theory and practical implementation of SPME. SPME protocols are presented outlining each stage of the method and providing useful tips and potential pitfalls. In addition, devices and fiber coatings, automated SPME systems, SPME method development, and In Vivo applications are discussed. This handbook is essential for its discussion of the latest SPME developments as well as its in depth information on the history, theory, and practical application of the method. Practical application of Solid Phase Microextraction methods including detailed steps Provides history of extraction methods to better understand the process Suitable for all levels, from beginning student to experienced practitioner
Book Synopsis Solid Phase Microextraction by : Gangfeng Ouyang
Download or read book Solid Phase Microextraction written by Gangfeng Ouyang and published by Springer. This book was released on 2016-11-24 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers comprehensive information on the developments and applications of the solid phase microextraction (SPME) technique. The first part of the book briefly introduces readers to the fundamentals of SPME, while subsequent sections describe the applications of SPME technique in detail, including environmental analysis (air, water, soil/sediments), food analysis (volatile/nonvolatile compounds), and bioanalysis (plants, animal tissues, body fluids). The advantages and future challenges of the SPME technique are also discussed. Including recent research advances and further developments of SPME, the book offers a practical reference guide and a valuable resource for researchers and users of SPME techniques. The target audience includes analytical chemists, environmental scientists, biological scientists, material scientists, and analysts, as well as students at universities/institutes in related fields. Dr. Gangfeng Ouyang is a Professor at the School of Chemistry and Chemical Engineering, Sun Yat-sen University, China. Dr. Ruifen Jiang is an Associate Professor at the School of Environment, Jinan University, China.
Book Synopsis Site-specific Fluorescent Derivatization of Peptides for Characterization by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Sara Jane Sudmeier
Download or read book Site-specific Fluorescent Derivatization of Peptides for Characterization by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Sara Jane Sudmeier and published by . This book was released on 1998 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by : Qifeng Xue
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by :
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by and published by . This book was released on 1995 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Ph. D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.
Book Synopsis Fluorescence detection techniques in liquid chromatography by : Lars Johnson
Download or read book Fluorescence detection techniques in liquid chromatography written by Lars Johnson and published by . This book was released on 1982 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Fluorescent Derivatization of Arginine- and Carbonyl-containing Analytes for Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection by :
Download or read book Fluorescent Derivatization of Arginine- and Carbonyl-containing Analytes for Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by and published by . This book was released on 2000 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Coupling of Solid-phase Extraction and Derivatization with Capillary Electrophoresis for Peptide Analysis by : Frans Willem Alexander Tempels
Download or read book Coupling of Solid-phase Extraction and Derivatization with Capillary Electrophoresis for Peptide Analysis written by Frans Willem Alexander Tempels and published by . This book was released on 2007 with total page 173 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Sample Preparation Techniques in Analytical Chemistry by : Somenath Mitra
Download or read book Sample Preparation Techniques in Analytical Chemistry written by Somenath Mitra and published by John Wiley & Sons. This book was released on 2004-04-07 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of accurate sample preparation techniques cannot be overstated--meticulous sample preparation is essential. Often overlooked, it is the midway point where the analytes from the sample matrix are transformed so they are suitable for analysis. Even the best analytical techniques cannot rectify problems generated by sloppy sample pretreatment. Devoted entirely to teaching and reinforcing these necessary pretreatment steps, Sample Preparation Techniques in Analytical Chemistry addresses diverse aspects of this important measurement step. These include: * State-of-the-art extraction techniques for organic and inorganic analytes * Sample preparation in biological measurements * Sample pretreatment in microscopy * Surface enhancement as a sample preparation tool in Raman and IR spectroscopy * Sample concentration and clean-up methods * Quality control steps Designed to serve as a text in an undergraduate or graduate level curriculum, Sample Preparation Techniques in Analytical Chemistry also provides an invaluable reference tool for analytical chemists in the chemical, biological, pharmaceutical, environmental, and materials sciences.
Book Synopsis High-throughput Analysis with 96-capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection by : Gang Xue
Download or read book High-throughput Analysis with 96-capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection written by Gang Xue and published by . This book was released on 2001 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. We first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. We further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10−11M (S/N=3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. A simple two-dimensional laser scanning scheme was also proposed with slight modification. 488 nm and 514 nm laser lines from a single Ar laser were alternatively scanned through the array to achieve two-color basecalling for successful high-throughput DNA sequencing. Finally, we addressed the sample preparation for the high-throughput DNA sequencing. An integrated system of nanoreactor for cycle-sequencing reaction coupled with online CZE purification and CGE DNA sequencing is presented. Less than 100 nl of cycle sequencing premixed reagent solution was hydrodynamically injected into a fused-silica capillary (75 [mu]m i.d.) inside a homemade microthermocycler for successful sample preparation, online purification, sequencing separation, and basecalling. The whole system is readily adaptable to automated multiplex operation for DNA sequencing or PCR analysis.
Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Book Synopsis Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides by : Guido Fridolin Verbeck
Download or read book Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides written by Guido Fridolin Verbeck and published by . This book was released on 1999 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection by :
Download or read book High-Throughput Analysis With 96-Capillary Array Electrophoresis and Integrated Sample Preparation for DNA Sequencing Based on Laser Induced Fluorescence Detection written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of this research was to improve the fluorescence detection for the multiplexed capillary array electrophoresis, extend its use beyond the genomic analysis, and to develop an integrated micro-sample preparation system for high-throughput DNA sequencing. The authors first demonstrated multiplexed capillary zone electrophoresis (CZE) and micellar electrokinetic chromatography (MEKC) separations in a 96-capillary array system with laser-induced fluorescence detection. Migration times of four kinds of fluoresceins and six polyaromatic hydrocarbons (PAHs) are normalized to one of the capillaries using two internal standards. The relative standard deviations (RSD) after normalization are 0.6-1.4% for the fluoresceins and 0.1-1.5% for the PAHs. Quantitative calibration of the separations based on peak areas is also performed, again with substantial improvement over the raw data. This opens up the possibility of performing massively parallel separations for high-throughput chemical analysis for process monitoring, combinatorial synthesis, and clinical diagnosis. The authors further improved the fluorescence detection by step laser scanning. A computer-controlled galvanometer scanner is adapted for scanning a focused laser beam across a 96-capillary array for laser-induced fluorescence detection. The signal at a single photomultiplier tube is temporally sorted to distinguish among the capillaries. The limit of detection for fluorescein is 3 x 10−11 M (S/N = 3) for 5-mW of total laser power scanned at 4 Hz. The observed cross-talk among capillaries is 0.2%. Advantages include the efficient utilization of light due to the high duty-cycle of step scan, good detection performance due to the reduction of stray light, ruggedness due to the small mass of the galvanometer mirror, low cost due to the simplicity of components, and flexibility due to the independent paths for excitation and emission.
Book Synopsis Handbook of Forensic Drug Analysis by : Fred Smith
Download or read book Handbook of Forensic Drug Analysis written by Fred Smith and published by Elsevier. This book was released on 2004-12-31 with total page 585 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Forensic Drug Analysis is a comprehensive chemical and analytic reference for the forensic analysis of illicit drugs. With chapters written by leading researchers in the field, the book provides in-depth, up-to-date methods and results of forensic drug analyses. This Handbook discusses various forms of the drug as well as the origin and nature of samples. It explains how to perform various tests, the use of best practices, and the analysis of results. Numerous forensic and chemical analytic techniques are covered including immunoassay, gas chromatography, and mass spectrometry. Topics range from the use of immunoassay technologies for drugs-of-abuse testing, to methods of forensic analysis for cannabis, hallucinogens, cocaine, opioids, and amphetamine. The book also looks at synthetic methods and law enforcement concerns regarding the manufacture of illicit drugs, with an emphasis on clandestine methamphetamine production. This Handbook should serve as a widely used reference for forensic scientists, toxicologists, pharmacologists, drug companies, and professionals working in toxicology testing labs, libraries, and poison control centers. It may also be used by chemists, physicians and those in legal and regulatory professions, and students of graduate courses in forensic science. Contributed to by leading scientists from around the world The only analysis book dedicated to illicit drugs of abuse Comprehensive coverage of sampling methods and various forms of analysis
Book Synopsis Handbook of Food Analysis - Two Volume Set by : Leo M.L. Nollet
Download or read book Handbook of Food Analysis - Two Volume Set written by Leo M.L. Nollet and published by CRC Press. This book was released on 2015-06-10 with total page 1530 pages. Available in PDF, EPUB and Kindle. Book excerpt: Updated to reflect changes in the industry during the last ten years, The Handbook of Food Analysis, Third Edition covers the new analysis systems, optimization of existing techniques, and automation and miniaturization methods. Under the editorial guidance of food science pioneer Leo M.L. Nollet and new editor Fidel Toldra, the chapters take an in
