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Oecd Guidelines For The Testing Of Chemicals Section 2 Test No 241 The Larval Amphibian Growth And Development Assay Lagda
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Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA) by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA) written by OECD and published by OECD Publishing. This book was released on 2015-07-28 with total page 38 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline describes the Medaka Extended One Generation Test (MEOGRT), which exposes fish over multiple generations to give data relevant to ecological hazard and risk assessment of chemicals, including suspected endocrine disrupting chemicals (EDCs). Exposure in the MEOGRT starts with ...
Book Synopsis Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA) by :
Download or read book Test No. 241: The Larval Amphibian Growth and Development Assay (LAGDA) written by and published by . This book was released on 2015 with total page 38 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 231: Amphibian Metamorphosis Assay by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 231: Amphibian Metamorphosis Assay written by OECD and published by OECD Publishing. This book was released on 2009-09-08 with total page 33 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline describes an amphibian metamorphosis assay intended to screen substances which may interfere with the normal functioning of the hypothalamo-pituitary-thyroid axis. The assay was validated with the species Xenopus laevis, which is ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA) by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 248: Xenopus Eleutheroembryonic Thyroid Assay (XETA) written by OECD and published by OECD Publishing. This book was released on 2019-06-18 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: The purpose of the test is to measure the capacity of a chemical to activate or inhibit the transcription of a genetic construct (THb/ZIP-GFP X. laevis eleutheroembryos) either by binding to the thyroid hormone receptor or by modifying the amount of thyroid hormone available for transcription. The test is based on the use of a transgenic frog (possessing the genetic construction THb/ZIP-GFP) of the species X. laevis. GFP is a fluorescent protein that can be used to affirm the presence of the substance and to follow its action. This test is based on the quantification of fluorescence, and thus of the GFP protein, throughout the eleutheroembryo exposed to a test chemical. Indeed, if the fluorescence is > 12% by including the highest concentration of the test, the test is considered positive. Organisms are exposed in chemically inert cell culture plates (6 wells). Each well contains ten organisms in solution. In a run, 20 organisms are exposed to each test concentration. Tadpoles are exposed to the test chemical at NF stage 45 for a duration of 72 hours. A positive result of the XETA test indicates in vivo that a substance induces damage to the receptor and metabolization of the thyroid hormone. A negative result indicates that, under test conditions, the test substance does not induce detectable damage to the thyroid and its functioning.
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 234: Fish Sexual Development Test by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 234: Fish Sexual Development Test written by OECD and published by OECD Publishing. This book was released on 2011-07-28 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline describes an assay that assesses early life-stage effects and potential adverse consequences of putative endocrine disrupting chemicals (e.g. oestrogens, androgens and steroidogenesis inhibitors) on fish sexual development. In ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 215: Fish, Juvenile Growth Test by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 215: Fish, Juvenile Growth Test written by OECD and published by OECD Publishing. This book was released on 2000-01-21 with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline is designed to assess the effects of prolonged exposure to chemicals on the growth of juvenile fish. Juvenile fish in exponential growth phase are placed, after being weighed, in test chambers and are normally exposed to five ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 210: Fish, Early-life Stage Toxicity Test by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 210: Fish, Early-life Stage Toxicity Test written by OECD and published by OECD Publishing. This book was released on 2013-07-26 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt: The test method described in this Test Guideline, is intended to define the lethal and sub-lethal effects of chemicals on the early life stages of the species tested. The early-life stages of fish are exposed to five concentrations of the test ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 229: Fish Short Term Reproduction Assay by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 229: Fish Short Term Reproduction Assay written by OECD and published by OECD Publishing. This book was released on 2012-10-02 with total page 40 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline describes an in vivo screening assay for fish reproduction where sexually mature male and spawning female fish are held together and exposed to a chemical during a limited part of their life-cycle (21 days). The short term ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 249: Fish Cell Line Acute Toxicity - The RTgill-W1 cell line assay written by OECD and published by OECD Publishing. This book was released on 2021-06-18 with total page 51 pages. Available in PDF, EPUB and Kindle. Book excerpt: The RTgill-W1 cell line assay describes a 24-well plate format fish cell line acute toxicity test using the permanent cell line from rainbow trout (Oncorhynchus mykiss) gill, RTgill-W1. After 24 h of exposure to the test chemical, cell viability is assessed based on three fluorescent cell viability indicator dyes, measured on the same set of cells. Resazurin enters the cells in its non-fluorescent form and is converted to the fluorescent product, resorufin, by mitochondrial, microsomal or cytoplasmic oxidoreductases.
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 232: Collembolan Reproduction Test in Soil by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 232: Collembolan Reproduction Test in Soil written by OECD and published by OECD Publishing. This book was released on 2016-07-29 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline is designed for assessing the effects of chemicals on the reproduction of collembolans in soil.
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 237: Honey Bee (Apis Mellifera) Larval Toxicity Test, Single Exposure written by OECD and published by OECD Publishing. This book was released on 2013-07-26 with total page 10 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline (TG) describes a honey bee brood acute toxicity test under laboratory conditions. The method aims at the determination of the lethal dose (72-h LD50) following single exposure of larvae to a chemical. On day 1 (D1) of the study ...
Book Synopsis Aquatic Toxicology in Freshwater by : Muhammed Atamanalp
Download or read book Aquatic Toxicology in Freshwater written by Muhammed Atamanalp and published by Springer Nature. This book was released on with total page 189 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 221: Lemna sp. Growth Inhibition Test by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 221: Lemna sp. Growth Inhibition Test written by OECD and published by OECD Publishing. This book was released on 2006-07-11 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline is designed to assess the toxicity of substances to freshwater aquatic plants of the genus Lemna (duckweed). Exponentially growing plant cultures of the genus Lemna (Lemna gibba and Lemna minor usually) are allowed to grow as ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 251: Rapid Androgen Disruption Activity Reporter (RADAR) assay by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 251: Rapid Androgen Disruption Activity Reporter (RADAR) assay written by OECD and published by OECD Publishing. This book was released on 2022-06-30 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Rapid Androgen Disruption Activity Reporter (RADAR) Test Guideline describes an aquatic assay that utilizes transgenic Oryzias latipes (O. latipes, Japanese medaka) eleutheroembryos at day post hatch zero, in a multi-well format to detect chemicals active on the androgen axis.
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 208: Terrestrial Plant Test: Seedling Emergence and Seedling Growth Test written by OECD and published by OECD Publishing. This book was released on 2006-08-17 with total page 21 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline is designed to assess effects on seedling emergence and early growth of higher plants following exposure to the test substance applied to the soil surface or into the soil. Seeds are placed in contact with soil treated with the ...
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 223: Avian Acute Oral Toxicity Test by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 223: Avian Acute Oral Toxicity Test written by OECD and published by OECD Publishing. This book was released on 2016-07-29 with total page 28 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Test Guideline describes procedures designed to estimate the acute oral toxicity of substances to birds, and it provides three testing options: (1) limit dose test, (2) LD50-slope test, and (3) LD50-only test. The LD50-slope and LD50-only options are sequential testing procedures.
Book Synopsis OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos by : OECD
Download or read book OECD Guidelines for the Testing of Chemicals, Section 2 Test No. 250: EASZY assay - Detection of Endocrine Active Substances, acting through estrogen receptors, using transgenic tg(cyp19a1b:GFP) Zebrafish embrYos written by OECD and published by OECD Publishing. This book was released on 2021-06-17 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt: The EASZY assay is a mechanism-based in vivo screening assay designed to detect endocrine active chemicals acting as agonist through estrogen receptors (ERs), by inducing the expression of the green fluorescent protein (GFP) driven by the cyp19a1b promoter. The EASZY assay allows for the detection of estrogenic activity of chemicals on transgenic tg(cyp19a1b:GFP) Zebrafish embryos exposed for 96 hours during the embryonic stages of development.