Non-Radioactive Labelling

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Author :
Publisher : Elsevier
ISBN 13 : 0080551092
Total Pages : 155 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Non-Radioactive Labelling by : A. J. Garman

Download or read book Non-Radioactive Labelling written by A. J. Garman and published by Elsevier. This book was released on 1997-03-20 with total page 155 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological Techniques is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers.In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series.The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work.Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels.This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. Aimed at researchers without specific expertise in chemistry, the book includes: A review of the main signal systems and labels available, indicating their strengths and weaknesses Discussion of the most useful strategies for labelling the various biomolecules 32 protocols covering common labelling needs Descriptions of the factors governing protocol design, enabling protocols to be modified for different applications Sources of information including references, data and suppliers

Non-Radioactive Labelling

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (742 download)

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Book Synopsis Non-Radioactive Labelling by :

Download or read book Non-Radioactive Labelling written by and published by . This book was released on 1997 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "Biological Techniques" is a series of volumes aimed at introducing to a wide audience the latest advances in methodology. The pitfalls and problems of new techniques are given due consideration, as are those small but vital details not always explicit in the methods sections of journal papers. In recent years, most biological laboratories have been invaded by computers and a wealth of new DNA technology and this will be reflected in many of the titles appearing in the series. The books will be of value to advanced researchers and graduate students seeking to learn and apply new techniques, and will be useful to teachers of advanced undergraduate courses involving practical or project work. Labelled biomolecules are an essential tool in life science research, and non-radioactive labels are becoming increasingly important due to their convenience of measurement, greater safety and lack of disposal problems compared to radioactive labels. This book provides practical information, background theory and protocols to allow a beginner to label many types of biomolecules, including proteins, peptides, nucleic acids and small molecules.; This book is essential for biochemists, molecular biologists and cell biologists wanting to use non-radioactively labelled molecules. It is aimed at researchers without specific expertise in chemistry, the book includes: a review of the main signal systems and labels available, indicating their strengths and weaknesses. It includes a discussion of the most useful strategies for labelling the various biomolecules. There are 32 protocols covering common labelling needs. There are descriptions of the factors governing protocol design, enabling protocols to be modified for different applications. It contains sources of information including references, data and suppliers.

Non-radioactive Labelling of Oligonucleotides

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Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (65 download)

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Book Synopsis Non-radioactive Labelling of Oligonucleotides by : John Grzybowski

Download or read book Non-radioactive Labelling of Oligonucleotides written by John Grzybowski and published by . This book was released on 1994 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Non-radioactive Labelling of Oligonucleotides

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Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (65 download)

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Book Synopsis Non-radioactive Labelling of Oligonucleotides by : John Grzybowski

Download or read book Non-radioactive Labelling of Oligonucleotides written by John Grzybowski and published by . This book was released on 1994 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Radioactive and Non-Radioactive Labelling

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Publisher :
ISBN 13 : 9780471965282
Total Pages : pages
Book Rating : 4.9/5 (652 download)

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Book Synopsis Radioactive and Non-Radioactive Labelling by : Buckley

Download or read book Radioactive and Non-Radioactive Labelling written by Buckley and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nonradioactive Labeling and Detection of Biomolecules

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Author :
Publisher : Springer
ISBN 13 : 9783540554820
Total Pages : 436 pages
Book Rating : 4.5/5 (548 download)

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Book Synopsis Nonradioactive Labeling and Detection of Biomolecules by : Christoph Kessler

Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 1992 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques inone source! This book gathers together all the importantnonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids likeDigoxigenin: Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, SilverEnhancement, and Synthetic Nucleic Acid Probe (SNAP) as wellas the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescentdetection.Additionally, applications for the use of non-isotopicallylabeled biomolecules are described. Specific protocols aregiven for hybridization analysis such as blot, colony/plaqueand in-situ hybridization formats, quantitative formats, andalso nonradioactivetechniques for nucleic acid sequencingand amplification.Each chapter contains a short introduction, a detaileddescription of the method with labprotocols, troubleshooting tips and references.

Strategies for Introducing Non-radioactive Labels During the Automated Sequence Analysis of Nucleic Acids

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Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (122 download)

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Book Synopsis Strategies for Introducing Non-radioactive Labels During the Automated Sequence Analysis of Nucleic Acids by : Gabor L. Igloi

Download or read book Strategies for Introducing Non-radioactive Labels During the Automated Sequence Analysis of Nucleic Acids written by Gabor L. Igloi and published by . This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Emergency Response Guidebook

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Publisher : Simon and Schuster
ISBN 13 : 1626363765
Total Pages : 400 pages
Book Rating : 4.6/5 (263 download)

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Book Synopsis Emergency Response Guidebook by : U.S. Department of Transportation

Download or read book Emergency Response Guidebook written by U.S. Department of Transportation and published by Simon and Schuster. This book was released on 2013-06-03 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: Does the identification number 60 indicate a toxic substance or a flammable solid, in the molten state at an elevated temperature? Does the identification number 1035 indicate ethane or butane? What is the difference between natural gas transmission pipelines and natural gas distribution pipelines? If you came upon an overturned truck on the highway that was leaking, would you be able to identify if it was hazardous and know what steps to take? Questions like these and more are answered in the Emergency Response Guidebook. Learn how to identify symbols for and vehicles carrying toxic, flammable, explosive, radioactive, or otherwise harmful substances and how to respond once an incident involving those substances has been identified. Always be prepared in situations that are unfamiliar and dangerous and know how to rectify them. Keeping this guide around at all times will ensure that, if you were to come upon a transportation situation involving hazardous substances or dangerous goods, you will be able to help keep others and yourself out of danger. With color-coded pages for quick and easy reference, this is the official manual used by first responders in the United States and Canada for transportation incidents involving dangerous goods or hazardous materials.

Basic Techniques in Molecular Biology

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Publisher : Springer Science & Business Media
ISBN 13 : 3642569684
Total Pages : 442 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Basic Techniques in Molecular Biology by : Stefan Surzycki

Download or read book Basic Techniques in Molecular Biology written by Stefan Surzycki and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory manual gives a thorough introduction to basic techniques. It is the result of practical experience, with each protocol having been used extensively in undergraduate courses or tested in the authors laboratory. In addition to detailed protocols and practical notes, each technique includes an overview of its general importance, the time and expense involved in its application and a description of the theoretical mechanisms of each step. This enables users to design their own modifications or to adapt the method to different systems. Surzycki has been holding undergraduate courses and workshops for many years, during which time he has extensively modified and refined the techniques described here.

The Development and Use of a Non-radioactive Iodine-labelling System for Microassays

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Publisher :
ISBN 13 :
Total Pages : 125 pages
Book Rating : 4.:/5 (87 download)

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Book Synopsis The Development and Use of a Non-radioactive Iodine-labelling System for Microassays by : Paula Keating

Download or read book The Development and Use of a Non-radioactive Iodine-labelling System for Microassays written by Paula Keating and published by . This book was released on 1991 with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nonradioactive Analysis of Biomolecules

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Publisher : Springer Science & Business Media
ISBN 13 : 9783540646013
Total Pages : 1170 pages
Book Rating : 4.6/5 (46 download)

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Book Synopsis Nonradioactive Analysis of Biomolecules by : Christoph Kessler

Download or read book Nonradioactive Analysis of Biomolecules written by Christoph Kessler and published by Springer Science & Business Media. This book was released on 2000-03-11 with total page 1170 pages. Available in PDF, EPUB and Kindle. Book excerpt: Highly sensitive systems which are widely used in molecular biological & biomedical laboratories, such as colorimetric, luminescence, fluorescence measuring using antibody-antigen binding or hybridisation, as well as PCR amplification are described in detail.

Molecular Biology LabFax

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Publisher : Elsevier
ISBN 13 : 9780121361105
Total Pages : 290 pages
Book Rating : 4.3/5 (611 download)

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Book Synopsis Molecular Biology LabFax by : T. A. Brown

Download or read book Molecular Biology LabFax written by T. A. Brown and published by Elsevier. This book was released on 1998-07-28 with total page 290 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 2.

Microbial Biotechnology

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Publisher : World Scientific
ISBN 13 : 9812566767
Total Pages : 812 pages
Book Rating : 4.8/5 (125 download)

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Book Synopsis Microbial Biotechnology by : Yuan Kun Lee

Download or read book Microbial Biotechnology written by Yuan Kun Lee and published by World Scientific. This book was released on 2006 with total page 812 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the second edition of this bestselling textbook, new materials have been added, including a new chapter on real time polymerase chain reaction (RTPCR) and a chapter on fungal solid state cultivation. There already exist a number of excellent general textbooks on microbiology and biotechnology that deal with the basic principles of microbial biotechnology. To complement them, this book focuses on the various applications of microbial-biotechnological principles. A teaching-based format is adopted, whereby working problems, as well as answers to frequently asked questions, supplement the main text. The book also includes real life examples of how the application of microbial-biotechnological principles has achieved breakthroughs in both research and industrial production.Although written for polytechnic students and undergraduates, the book contains sufficient information to be used as a reference for postgraduate students and lecturers. It may also serve as a resource book for corporate planners, managers and applied research personnel.

Nonradioactive Labeling and Detection of Biomolecules

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Author :
Publisher : Springer
ISBN 13 : 3662001446
Total Pages : 449 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Nonradioactive Labeling and Detection of Biomolecules by : Christoph Kessler

Download or read book Nonradioactive Labeling and Detection of Biomolecules written by Christoph Kessler and published by Springer. This book was released on 2012-12-06 with total page 449 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the bioanalytical labeling and detection techniques in one source! This book gathers together all the important nonradioactive labeling techniques for nucleic acids, proteins, glycoproteins and glycolipids like Digoxigenin:Anti-Dioxigenin (DIG), Biotin, 5-Bromodeoxyuridine (BrdU), Sulfone, Immunogold, Silver Enhancement, and Synthetic Nucleic Acid Probe (SNAP) as well as the standard procedures for optical, chemical, biological, electrochemiluminescent and fluorescent detection. Additionally, applications for the use of non-isotopically labeled biomolecules are described. Specific protocols are given for hybridization analysis such as blot, colony/plaque and in-situ hybridization formats, quantitative formats, and also nonradioactivetechniques for nucleic acid sequencing and amplification. Each chapter contains a short introduction, a detailed description of the method with labprotocols, troubleshooting tips and references.

Methods In Biotechnology

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Publisher : CRC Press
ISBN 13 : 9780748404308
Total Pages : 276 pages
Book Rating : 4.4/5 (43 download)

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Book Synopsis Methods In Biotechnology by : Michael Schweizer

Download or read book Methods In Biotechnology written by Michael Schweizer and published by CRC Press. This book was released on 1997-03-10 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a grounding in the experimental techniques applicable to the discipline of biotechnology. The introductory section in the text describes procedures for analysis of inorganic and organic materials, strain maintenance and fundamental experiments in gene manipulation. Other chapters deal with fermentation techniques, purification methods for substances of interest, preparation of microbial sensors and the demonstration of oil degradation by bacteria. The final chapter deals with statistical planning of experiments and scale-up methods.

RNA Methodologies

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Publisher : Elsevier
ISBN 13 : 0080454763
Total Pages : 794 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis RNA Methodologies by : Robert E. Farrell Jr.

Download or read book RNA Methodologies written by Robert E. Farrell Jr. and published by Elsevier. This book was released on 2010-07-22 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt: This laboratory guide represents a growing collection of tried, tested and optimized laboratory protocols for the isolation and characterization of eukaryotic RNA, with lesser emphasis on the characterization of prokaryotic transcripts. Collectively the chapters work together to embellish the RNA story, each presenting clear take-home lessons, liberally incorporating flow charts, tables and graphs to facilitate learning and assist in the planning and implementation phases of a project. RNA Methodologies, 3rd edition includes approximately 30% new material, including chapters on the more recent technologies of RNA interference including: RNAi; Microarrays; Bioinformatics. It also includes new sections on: new and improved RT-PCR techniques; innovative 5’ and 3’ RACE techniques; subtractive PCR methods; methods for improving cDNA synthesis. * Author is a well-recognized expert in the field of RNA experimentation and founded Exon-Intron, a well-known biotechnology educational workshop center * Includes classic and contemporary techniques * Incorporates flow charts, tables, and graphs to facilitate learning and assist in the planning phases of projects

Gene Cloning and DNA Analysis

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Publisher : John Wiley & Sons
ISBN 13 : 1444334077
Total Pages : 337 pages
Book Rating : 4.4/5 (443 download)

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Book Synopsis Gene Cloning and DNA Analysis by : Terry Brown

Download or read book Gene Cloning and DNA Analysis written by Terry Brown and published by John Wiley & Sons. This book was released on 2010-04-19 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Known world-wide as the standard introductory text to this important and exciting area, the sixth edition of Gene Cloning and DNA Analysis addresses new and growing areas of research whilst retaining the philosophy of the previous editions. Assuming the reader has little prior knowledge of the subject, its importance, the principles of the techniques used and their applications are all carefully laid out, with over 250 clearly presented four-colour illustrations. In addition to a number of informative changes to the text throughout the book, the final four chapters have been significantly updated and extended to reflect the striking advances made in recent years in the applications of gene cloning and DNA analysis in biotechnology. Gene Cloning and DNA Analysis remains an essential introductory text to a wide range of biological sciences students; including genetics and genomics, molecular biology, biochemistry, immunology and applied biology. It is also a perfect introductory text for any professional needing to learn the basics of the subject. All libraries in universities where medical, life and biological sciences are studied and taught should have copies available on their shelves. "… the book content is elegantly illustrated and well organized in clear-cut chapters and subsections… there is a Further Reading section after each chapter that contains several key references… What is extremely useful, almost every reference is furnished with the short but distinct author's remark." –Journal of Heredity, 2007 (on the previous edition)