Multiple Detection In Size Exclusion Chromatography

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Multiple Detection in Size-exclusion Chromatography

Author : André M. Striegel
Publisher :
ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Multiple Detection in Size-exclusion Chromatography by : André M. Striegel

Download or read book Multiple Detection in Size-exclusion Chromatography written by André M. Striegel and published by . This book was released on 2005 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: For four decades, size-exclusion chromatography has played a prominent role in characterizing polymers, by determining the polymer's molar mass averages, often not absolutely but relative to some calibration standard. Not satisfied, scientists now want to determine the absolute molar mass averages and distribution and to characterize their long-chain and short-chain branching, tacticity, copolymer and base-pair sequences, and other matters. Oh yes, and they want all this as a continuous function of the molar mass of the analyte. Fortunately, the technique is endowed with a number of detection methods, and the 18 papers here explain the role of those various detection methods and the synergistic effect of combining them in different configurations to obtain desired results. Distributed in the US by Oxford University Press. Annotation : 2004 Book News, Inc., Portland, OR (booknews.com).

Handbook Of Size Exclusion Chromatography And Related Techniques

Author : Chi-San Wu
Publisher : CRC Press
ISBN 13 : 0824757793
Total Pages : 721 pages
Book Rating : 4.8/5 (247 download)

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Book Synopsis Handbook Of Size Exclusion Chromatography And Related Techniques by : Chi-San Wu

Download or read book Handbook Of Size Exclusion Chromatography And Related Techniques written by Chi-San Wu and published by CRC Press. This book was released on 2003-11-04 with total page 721 pages. Available in PDF, EPUB and Kindle. Book excerpt: Documenting critical advances in this rapidly evolving field, the Second Edition highlights the need for new applications and technologies that assist in the determination of molecular weight and molecular weight distributions of polymers in an accurate, efficient manner. This volume presents the latest findings from a international team of specialists and continues to inspire and extend practical applications of size exclusion chromatography (SEC). It includes six new chapters covering high-speed size exclusion chromatography, SEC of low molecular weight materials, and the extended family of techniques, from two-dimensional liquid chromatography to high osmotic pressure chromatography.

Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation

Author : Stepan Podzimek
Publisher : John Wiley & Sons
ISBN 13 : 111810272X
Total Pages : 325 pages
Book Rating : 4.1/5 (181 download)

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Book Synopsis Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation by : Stepan Podzimek

Download or read book Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation written by Stepan Podzimek and published by John Wiley & Sons. This book was released on 2011-04-20 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive, practical approach to three powerful methods of polymer analysis and characterization This book serves as a complete compendium of three important methods widely used for the characterization of synthetic and natural polymers—light scattering, size exclusion chromatography (SEC), and asymmetric flow field flow fractionation (A4F). Featuring numerous up-to-date examples of experimental results obtained by light scattering, SEC, and A4F measurements, Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation takes an all-in-one approach to deliver a complete and thorough explanation of the principles, theories, and instrumentation needed to characterize polymers from the viewpoint of their molar mass distribution, size, branching, and aggregation. This comprehensive resource: Is the only book gathering light scattering, size exclusion chromatography, and asymmetric flow field flow fractionation into a single text Systematically compares results of size exclusion chromatography with results of asymmetric flow field flow fractionation, and how these two methods complement each other Provides in-depth guidelines for reproducible and correct determination of molar mass and molecular size of polymers using SEC or A4F coupled with a multi-angle light scattering detector Offers a detailed overview of the methodology, detection, and characterization of polymer branching Light Scattering, Size Exclusion Chromatography and Asymmetric Flow Field Flow Fractionation should be of great interest to all those engaged in the polymer analysis and characterization in industrial and university research, as well as in manufacturing quality control laboratories. Both beginners and experienced can confidently rely on this volume to confirm their own understanding or to help interpret their results.

Modern Size-Exclusion Liquid Chromatography

Author : Andre Striegel
Publisher : John Wiley & Sons
ISBN 13 : 0470442832
Total Pages : 512 pages
Book Rating : 4.4/5 (74 download)

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Book Synopsis Modern Size-Exclusion Liquid Chromatography by : Andre Striegel

Download or read book Modern Size-Exclusion Liquid Chromatography written by Andre Striegel and published by John Wiley & Sons. This book was released on 2009-07-31 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Second Edition of Modern Size-Exclusion Chromatography offers a complete guide to the theories, methods, and applications of size-exclusion chromatography. It provides an unparalleled, integrated, up-to-date treatment of gel permeation and gel filtration chromatography. With its detailed descriptions of techniques, data handling, compilations of information on columns and column packings, and tables of important solvents and reference materials, the book offers readers everything they need to take full advantage of this popular macromolecular characterization technique. Since publication of the first edition in 1979, there have been many important advances in the field of size-exclusion chromatography. This Second Edition brings the book thoroughly up to date, with expert coverage of: New and emerging industrial and research applications Practical aspects of size-exclusion chromatography (SEC) and multidetector and multidimensional SEC technologies for polymer architecture and copolymer analysis Updated information on the latest equipment and techniques New best practices for the lab SEC in relation to polymer characterization techniques such as GPEC, LCCC, and rheology Throughout the text, detailed examples guide you step by step through all the latest techniques and applications. With its extensive revisions and updates written by leading experts and pioneers in the field, Modern Size-Exclusion Liquid Chromatography remains the definitive resource for the broad range of researchers and scientists who use HPLC and GPC methods.

Single-Chain Polymer Nanoparticles

Author : José A. Pomposo
Publisher : John Wiley & Sons
ISBN 13 : 3527806393
Total Pages : 312 pages
Book Rating : 4.5/5 (278 download)

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Book Synopsis Single-Chain Polymer Nanoparticles by : José A. Pomposo

Download or read book Single-Chain Polymer Nanoparticles written by José A. Pomposo and published by John Wiley & Sons. This book was released on 2017-08-18 with total page 312 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on this important and emerging topic presents an overview of the very latest results obtained in single-chain polymer nanoparticles obtained by folding synthetic single polymer chains, painting a complete picture from synthesis via characterization to everyday applications. The initial chapters describe the synthetics methods as well as the molecular simulation of these nanoparticles, while subsequent chapters discuss the analytical techniques that are applied to characterize them, including size and structural characterization as well as scattering techniques. The final chapters are then devoted to the practical applications in nanomedicine, sensing, catalysis and several other uses, concluding with a look at the future for such nanoparticles. Essential reading for polymer and materials scientists, materials engineers, biochemists as well as environmental chemists.

Mass Spectrometry in Polymer Chemistry

Author : Christopher Barner-Kowollik
Publisher : John Wiley & Sons
ISBN 13 : 3527641831
Total Pages : 501 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Mass Spectrometry in Polymer Chemistry by : Christopher Barner-Kowollik

Download or read book Mass Spectrometry in Polymer Chemistry written by Christopher Barner-Kowollik and published by John Wiley & Sons. This book was released on 2012-01-27 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: Combining an up-to-date insight into mass-spectrometric polymer analysis beyond MALDI with application details of the instrumentation, this is a balanced and thorough presentation of the most important and widely used mass-spectrometric methods. Written by the world's most proficient experts in the field, the book focuses on the latest developments, covering such technologies and applications as ionization protocols, tandem and liquid chromatography mass spectrometry, gas-phase ion-separation techniques and automated data processing. Chapters on sample preparation, polymer degradation and the usage of mass-spectrometric tools on an industrial scale round off the book. As a result, both entrants to the field and experienced researchers are able to choose the appropriate methods and instrumentations -- and to assess their respective strengths and limitations -- for the characterization of polymer compounds.

Multidimensional HPLC of Polymers

Author : Harald Pasch
Publisher : Springer Science & Business Media
ISBN 13 : 3642360807
Total Pages : 291 pages
Book Rating : 4.6/5 (423 download)

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Book Synopsis Multidimensional HPLC of Polymers by : Harald Pasch

Download or read book Multidimensional HPLC of Polymers written by Harald Pasch and published by Springer Science & Business Media. This book was released on 2013-06-12 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the principle ideas of combining different analytical techniques in multi-dimensional analysis schemes. It reviews the basic principles and instrumentation of multi-dimensional chromatography and the hyphenation of liquid chromatography with selective spectroscopic detectors and presents experimental protocols for the analysis of complex polymers. It is the consequent continuation of "HPLC of Polymers" from 1999 by the same authors. Like its 'predecessor', this book discusses the theoretical background, equipment, experimental procedures and applications for each separation technique, but in contrast treats multi-dimensional and coupled techniques. "Multidimensional HPLC of Polymers" intends to review the state of the art in polymer chromatography and to summarize the developments in the field during the last 15 years. With its tutorial and laboratory manual style it is written for beginners as well as for experienced chromatographers, and will enable its readers (polymer chemists, physicists and material scientists, as well as students of polymer and analytical sciences) to optimize the experimental conditions for their specific separation problems.

Detection and Data Analysis in Size Exclusion Chromatography

Author : Theodore Provder
Publisher :
ISBN 13 :
Total Pages : 378 pages
Book Rating : 4.:/5 (44 download)

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Book Synopsis Detection and Data Analysis in Size Exclusion Chromatography by : Theodore Provder

Download or read book Detection and Data Analysis in Size Exclusion Chromatography written by Theodore Provder and published by . This book was released on 1987 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Size Exclusion Chromatography

Author : B. J. Hunt
Publisher : Springer Science & Business Media
ISBN 13 : 1461578612
Total Pages : 292 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Size Exclusion Chromatography by : B. J. Hunt

Download or read book Size Exclusion Chromatography written by B. J. Hunt and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is a large and increasing variety of polymers currently in use both for domestic and industrial applications. The properties of polymers are deter mined not only by their chemical type, but also by their molecular mass and molecular mass distributions. However, while the chemical type of polymers can be determined relatively easily, the average molecular masses and molecular mass distributions are more difficult to measure. The molecular mass averages of a polymer are measured by specialized and complex techniques such as light scattering (for weight average) and osmometry (for number average). Thus, complete characterization of the molecular mass distribution of a polymer by such means requires separating the sample into many fractions which can then be examined individually. Since size exclusion chromatography was introduced as a rapid and straightforward technique for the characterization of polymer molecular mass distributions, there have been tremendous increases in development and applications, and it was felt appropriate to bring together into a single volume the information required by scientists from many disciplines who wish to use the technique. This book should be useful to existing users, those who are new to the technique, and those who may be familiar with the basic technique and now wish to extend their capabilities to more complex applications (or to consider the potential of a number of related techniques). The book will also be of general interest to the experienced liquid chromatographer.

Polymer Science: A Comprehensive Reference

Author :
Publisher : Newnes
ISBN 13 : 0080878628
Total Pages : 7752 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Polymer Science: A Comprehensive Reference by :

Download or read book Polymer Science: A Comprehensive Reference written by and published by Newnes. This book was released on 2012-12-05 with total page 7752 pages. Available in PDF, EPUB and Kindle. Book excerpt: The progress in polymer science is revealed in the chapters of Polymer Science: A Comprehensive Reference, Ten Volume Set. In Volume 1, this is reflected in the improved understanding of the properties of polymers in solution, in bulk and in confined situations such as in thin films. Volume 2 addresses new characterization techniques, such as high resolution optical microscopy, scanning probe microscopy and other procedures for surface and interface characterization. Volume 3 presents the great progress achieved in precise synthetic polymerization techniques for vinyl monomers to control macromolecular architecture: the development of metallocene and post-metallocene catalysis for olefin polymerization, new ionic polymerization procedures, and atom transfer radical polymerization, nitroxide mediated polymerization, and reversible addition-fragmentation chain transfer systems as the most often used controlled/living radical polymerization methods. Volume 4 is devoted to kinetics, mechanisms and applications of ring opening polymerization of heterocyclic monomers and cycloolefins (ROMP), as well as to various less common polymerization techniques. Polycondensation and non-chain polymerizations, including dendrimer synthesis and various "click" procedures, are covered in Volume 5. Volume 6 focuses on several aspects of controlled macromolecular architectures and soft nano-objects including hybrids and bioconjugates. Many of the achievements would have not been possible without new characterization techniques like AFM that allowed direct imaging of single molecules and nano-objects with a precision available only recently. An entirely new aspect in polymer science is based on the combination of bottom-up methods such as polymer synthesis and molecularly programmed self-assembly with top-down structuring such as lithography and surface templating, as presented in Volume 7. It encompasses polymer and nanoparticle assembly in bulk and under confined conditions or influenced by an external field, including thin films, inorganic-organic hybrids, or nanofibers. Volume 8 expands these concepts focusing on applications in advanced technologies, e.g. in electronic industry and centers on combination with top down approach and functional properties like conductivity. Another type of functionality that is of rapidly increasing importance in polymer science is introduced in volume 9. It deals with various aspects of polymers in biology and medicine, including the response of living cells and tissue to the contact with biofunctional particles and surfaces. The last volume is devoted to the scope and potential provided by environmentally benign and green polymers, as well as energy-related polymers. They discuss new technologies needed for a sustainable economy in our world of limited resources. Provides broad and in-depth coverage of all aspects of polymer science from synthesis/polymerization, properties, and characterization methods and techniques to nanostructures, sustainability and energy, and biomedical uses of polymers Provides a definitive source for those entering or researching in this area by integrating the multidisciplinary aspects of the science into one unique, up-to-date reference work Electronic version has complete cross-referencing and multi-media components Volume editors are world experts in their field (including a Nobel Prize winner)

Size Exclusion Chromatography

Author : Sadao Mori
Publisher : Springer Science & Business Media
ISBN 13 : 3662039109
Total Pages : 236 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Size Exclusion Chromatography by : Sadao Mori

Download or read book Size Exclusion Chromatography written by Sadao Mori and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although size exclusion chromatography (SEC) is perhaps the most popular and widely used technique for determining the molecular weight distribution of polymeric materials, there have been very few texts written on this topic. During the past decade, SEC has experienced a considerable amount of growth in regard to column and detector technology and new applications. With these advances, SEC can now be used for determining absolute molecular weight, polymer chain conformation and size, and branching, as well as polymer solution properties. This book introduces the reader to the fundamentals of SEC with emphasis on practical aspects of the technique, such as column and mobile selection, calibration, new detector capabilities and guidelines for performing SEC on most types of polymers, especially those of industrial importance. This book is intended for either those new to the field of SEC, or for those research workers who require a more comprehensive background.

Biological Small Angle Scattering: Techniques, Strategies and Tips

Author : Barnali Chaudhuri
Publisher : Springer
ISBN 13 : 981106038X
Total Pages : 268 pages
Book Rating : 4.8/5 (11 download)

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Book Synopsis Biological Small Angle Scattering: Techniques, Strategies and Tips by : Barnali Chaudhuri

Download or read book Biological Small Angle Scattering: Techniques, Strategies and Tips written by Barnali Chaudhuri and published by Springer. This book was released on 2017-12-07 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a clear, comprehensible and up-to-date description of how Small Angle Scattering (SAS) can help structural biology researchers. SAS is an efficient technique that offers structural information on how biological macromolecules behave in solution. SAS provides distinct and complementary data for integrative structural biology approaches in combination with other widely used probes, such as X-ray crystallography, Nuclear magnetic resonance, Mass spectrometry and Cryo-electron Microscopy. The development of brilliant synchrotron small-angle X-ray scattering (SAXS) beam lines has increased the number of researchers interested in solution scattering. SAS is especially useful for studying conformational changes in proteins, highly flexible proteins, and intrinsically disordered proteins. Small-angle neutron scattering (SANS) with neutron contrast variation is ideally suited for studying multi-component assemblies as well as membrane proteins that are stabilized in surfactant micelles or vesicles. SAS is also used for studying dynamic processes of protein fibrillation in amyloid diseases, and pharmaceutical drug delivery. The combination with size-exclusion chromatography further increases the range of SAS applications. The book is written by leading experts in solution SAS methodologies. The principles and theoretical background of various SAS techniques are included, along with practical aspects that range from sample preparation to data presentation for publication. Topics covered include techniques for improving data quality and analysis, as well as different scientific applications of SAS. With abundant illustrations and practical tips, we hope the clear explanations of the principles and the reviews on the latest progresses will serve as a guide through all aspects of biological solution SAS. The scope of this book is particularly relevant for structural biology researchers who are new to SAS. Advanced users of the technique will find it helpful for exploring the diversity of solution SAS methods and applications. Chapter 3 of this book is available open access under a CC BY 4.0 license at link.springer.com.

Column Handbook for Size Exclusion Chromatography

Author : Chi-san Wu
Publisher : Elsevier
ISBN 13 : 0080529399
Total Pages : 663 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Column Handbook for Size Exclusion Chromatography by : Chi-san Wu

Download or read book Column Handbook for Size Exclusion Chromatography written by Chi-san Wu and published by Elsevier. This book was released on 1999-03-19 with total page 663 pages. Available in PDF, EPUB and Kindle. Book excerpt: Column Handbook for Size Exclusion Chromatography is the first comprehensive reference to provide everything one needs to know about commercial analytical and preparative columns for size exclusion and gel filtration chromatography (SEC and GFC). SEC is now widely used as a quality assurance method in the polymer industry (both synthetic and biopolymers) to determine molecular weight and molecular weight distribution. The Handbook contains contributions from every column manufacturer around the world and from many experienced column users. It covers the technology, characterization, application, evaluation, maintenance, and quality control of analytical and preparative columns for SEC and GFC. Also included are columns for two closely related techniques, hydrodynamic chromatography and high osmotic pressure chromatography. Key Features* Evaluate and select columns with confidence for specific applications* Optimize separations and improve the ruggedness of analytical methods* Extend the service time of a column* Establish a quality-control program to ensure consistency in column performance* Avoid the expense of column damage or purchases that do not give the expected results

Liquid Chromatography

Author : Salvatore Fanali
Publisher : Elsevier
ISBN 13 : 0128093455
Total Pages : 808 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Liquid Chromatography by : Salvatore Fanali

Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-22 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. Emphasizes the integration of chromatographic methods and sample preparation Explains how liquid chromatography is used in different industrial sectors Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Quantification in LC and GC

Author : Stavros Kromidas
Publisher : John Wiley & Sons
ISBN 13 : 3527323015
Total Pages : 377 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Quantification in LC and GC by : Stavros Kromidas

Download or read book Quantification in LC and GC written by Stavros Kromidas and published by John Wiley & Sons. This book was released on 2009-10-12 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: Closing a gap in the current literature by addressing the evaluation and quality assessment of raw data, this practice-oriented guide is clearly divided into three parts. The first describes basic considerations of chromatographic data quality, common errors and potential pitfalls in reading out and quantifying the data. Part two systematically covers the most important chromatographic methods as well as the specific requirements for obtaining good chromatographic data. The final part looks at data quality from the perspective of those regulatory authorities demanding certain standards in data quality, describing in detail best practices. Written with the practitioner in mind, the text not only teaches the mathematical basics but also provides invaluable advice.

New and Emerging Proteomic Techniques

Author : Dobrin Nedelkov
Publisher : Springer Science & Business Media
ISBN 13 : 159745026X
Total Pages : 237 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis New and Emerging Proteomic Techniques by : Dobrin Nedelkov

Download or read book New and Emerging Proteomic Techniques written by Dobrin Nedelkov and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 237 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading researchers and innovators describe in step-by-step detail the latest techniques that promise to significantly impact the practice of proteomics, as well as its success in developing novel clinical agents. The methods span the entire spectrum of top-down and bottom-up approaches, including microarrays, gels, chromatography, and affinity separations, and address every aspect of the human proteome, both quantitatively and qualitatively. The techniques of protein detection utilized are diverse and range from fluorescence and resonance light scattering to surface plasmon resonance and mass spectrometry. The protocols follow the successful Methods in Molecular BiologyTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principles behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.

Handbook of Spectroscopy

Author : G¿nter Gauglitz
Publisher : John Wiley & Sons
ISBN 13 : 3527654720
Total Pages : 2011 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Handbook of Spectroscopy by : G¿nter Gauglitz

Download or read book Handbook of Spectroscopy written by G¿nter Gauglitz and published by John Wiley & Sons. This book was released on 2014-05-05 with total page 2011 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second, thoroughly revised, updated and enlarged edition provides a straightforward introduction to spectroscopy, showing what it can do and how it does it, together with a clear, integrated and objective account of the wealth of information that may be derived from spectra. It also features new chapters on spectroscopy in nano-dimensions, nano-optics, and polymer analysis. Clearly structured into sixteen sections, it covers everything from spectroscopy in nanodimensions to medicinal applications, spanning a wide range of the electromagnetic spectrum and the physical processes involved, from nuclear phenomena to molecular rotation processes. In addition, data tables provide a comparison of different methods in a standardized form, allowing readers to save valuable time in the decision process by avoiding wrong turns, and also help in selecting the instrumentation and performing the experiments. These four volumes are a must-have companion for daily use in every lab.