Multiphoton Microscopy Fluorescence Lifetime Imaging And Optical Spectroscopy For The Diagnosis Of Neoplasia

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Fluorescence Lifetime Spectroscopy and Imaging

Author : Laura Marcu
Publisher : CRC Press
ISBN 13 : 1439861676
Total Pages : 574 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu

Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.

Fluorescence Lifetime Imaging Ophthalmoscopy

Author : Martin Zinkernagel
Publisher : Springer
ISBN 13 : 3030228789
Total Pages : 123 pages
Book Rating : 4.0/5 (32 download)

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Book Synopsis Fluorescence Lifetime Imaging Ophthalmoscopy by : Martin Zinkernagel

Download or read book Fluorescence Lifetime Imaging Ophthalmoscopy written by Martin Zinkernagel and published by Springer. This book was released on 2019-07-29 with total page 123 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the emerging non-invasive imaging technique of Fluorescence Lifetime Imaging Ophthalmoscopy (FLIO). FLIO reveals unique information on retinal diseases, ranging from age-related macular degeneration and vascular diseases to hereditary retinal dystrophies. Fluorescence lifetimes enable the evaluation of disease progression before irreversible structural changes occur. The image acquisition is suitable for diagnostic purposes and follow-up examinations to investigate the natural course of disease, and to monitor the effects of possible therapies. This book fills the gap between available literature and gives state-of-the-art guidance on the principles of the FLIO technique, image acquisition, and data analysis. Written by a team of expert leaders within this field, this book will be relevant for scientists and clinicians with an interest in ophthalmoscopy.

Multiphoton Microscopy and Fluorescence Lifetime Imaging

Author : Karsten König
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 311043007X
Total Pages : 785 pages
Book Rating : 4.1/5 (14 download)

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Book Synopsis Multiphoton Microscopy and Fluorescence Lifetime Imaging by : Karsten König

Download or read book Multiphoton Microscopy and Fluorescence Lifetime Imaging written by Karsten König and published by Walter de Gruyter GmbH & Co KG. This book was released on 2018-01-22 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: This monograph focuses on modern femtosecond laser microscopes for two photon imaging and nanoprocessing, on laser tweezers for cell micromanipulation as well as on fluorescence lifetime imaging (FLIM) in Life Sciences. The book starts with an introduction by Dr. Wolfgang Kaiser, pioneer of nonlinear optics and ends with the chapter on clinical multiphoton tomography, the novel high resolution imaging technique. It includes a foreword by the nonlinear microscopy expert Dr. Colin Sheppard. Contents Part I: Basics Brief history of fluorescence lifetime imaging The long journey to the laser and its use for nonlinear optics Advanced TCSPC-FLIM techniques Ultrafast lasers in biophotonics Part II: Modern nonlinear microscopy of live cells STED microscopy: exploring fluorescence lifetime gradients for super-resolution at reduced illumination intensities Principles and applications of temporal-focusing wide-field two-photon microscopy FLIM-FRET microscopy TCSPC FLIM and PLIM for metabolic imaging and oxygen sensing Laser tweezers are sources of two-photon effects Metabolic shifts in cell proliferation and differentiation Femtosecond laser nanoprocessing Cryomultiphoton imaging Part III: Nonlinear tissue imaging Multiphoton Tomography (MPT) Clinical multimodal CARS imaging In vivo multiphoton microscopy of human skin Two-photon microscopy and fluorescence lifetime imaging of the cornea Multiscale correlative imaging of the brain Revealing interaction of dyes and nanomaterials by multiphoton imaging Multiphoton FLIM in cosmetic clinical research Multiphoton microscopy and fluorescence lifetime imaging for resection guidance in malignant glioma surgery Non-invasive single-photon and multi-photon imaging of stem cells and cancer cells in mouse models Bedside assessment of multiphoton tomography

Optical Spectroscopy And Imaging For Cancer Diagnostics: Fundamentals, Progress, And Challenges

Author : Noureddine Melikechi
Publisher : World Scientific
ISBN 13 : 981125897X
Total Pages : 559 pages
Book Rating : 4.8/5 (112 download)

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Book Synopsis Optical Spectroscopy And Imaging For Cancer Diagnostics: Fundamentals, Progress, And Challenges by : Noureddine Melikechi

Download or read book Optical Spectroscopy And Imaging For Cancer Diagnostics: Fundamentals, Progress, And Challenges written by Noureddine Melikechi and published by World Scientific. This book was released on 2023-01-06 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is an interdisciplinary book that presents the applications of novel laser spectroscopy and imaging techniques for the detection of cancers recently developed by some of the world's most renown researchers. The book consists of three parts and a total of 16 chapters. Each chapter is written by leading experts who are actively seeking to develop novel spectroscopic and analytical methods for cancer detection and diagnosis.In Part I, the authors present fundamentals on optics, atoms and molecules, biophysics, cancer and machine learning. These chapters are intended for those who are not experts in the field but wish to learn about fundamentals' aspects of some of the key topics that are addressed in this book. Particular attention has been given to providing key references for those who wish to go further into the fundamental aspects of atoms and molecules, light-matter interaction, optical instrumentation, machine learning and cancer.In Part II, the authors present key applications of various laser spectroscopic methods in cancer diagnosis. They have provided recent progress in cancer diagnostics obtained by combining laser spectroscopy and machine learning for the analysis of the spectra acquired from biomedical tissues and biofluids.In Part III, the authors present chapters that discuss key developments in the applications of various laser imaging techniques for cancer detection.This is one of the few books that addresses cancer detection and diagnosis using laser spectroscopic and imaging tools with an eye on providing the reader the scientific tools, including machine learning ones.

Fluorescence Lifetime Spectroscopy and Imaging

Author : Laura Marcu
Publisher : CRC Press
ISBN 13 : 1439861684
Total Pages : 554 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu

Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Dia

Multimodal Optical Diagnostics of Cancer

Author : Valery V. Tuchin
Publisher : Springer Nature
ISBN 13 : 3030445941
Total Pages : 605 pages
Book Rating : 4.0/5 (34 download)

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Book Synopsis Multimodal Optical Diagnostics of Cancer by : Valery V. Tuchin

Download or read book Multimodal Optical Diagnostics of Cancer written by Valery V. Tuchin and published by Springer Nature. This book was released on 2020-10-23 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides an in-depth description and discussion of different multi-modal diagnostic techniques for cancer detection and treatment using exact optical methods, their comparison, and combination. Coverage includes detailed descriptions of modern state of design for novel methods of optical non-invasive cancer diagnostics; multi-modal methods for earlier cancer diagnostic enhancing the probability of effective cancer treatment; modern clinical trials with novel methods of clinical cancer diagnostics; medical and technical aspects of clinical cancer diagnostics, and long-term monitoring. Biomedical engineers, cancer researchers, and scientists will find the book to be an invaluable resource. Introduces optical imaging strategies; Focuses on multimodal optical diagnostics as a fundamental approach; Discusses novel methods of optical non-invasive cancer diagnostics.

Natural Biomarkers for Cellular Metabolism

Author : Vladimir V. Ghukasyan
Publisher : CRC Press
ISBN 13 : 1466509988
Total Pages : 412 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Natural Biomarkers for Cellular Metabolism by : Vladimir V. Ghukasyan

Download or read book Natural Biomarkers for Cellular Metabolism written by Vladimir V. Ghukasyan and published by CRC Press. This book was released on 2014-09-26 with total page 412 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the Lab to Clinical Settings—Advances in Quantitative, Noninvasive Optical Diagnostics Noninvasive fluorescence imaging techniques, novel fluorescent labels, and natural biomarkers are revolutionizing our knowledge of cellular processes, signaling and metabolic pathways, the underlying mechanisms for health problems, and the identification of new therapeutic targets for drug discoveries. Natural Biomarkers for Cellular Metabolism: Biology, Techniques, and Applications delves into the current state of knowledge on intrinsic fluorescent biomarkers and highlights recent developments in using these biomarkers for the metabolic mapping and clinical diagnosis of healthy and diseased cells and tissues. Autofluorescent Biomarkers for Biomedical Diagnostics The book’s first section introduces the fundamentals of cellular energy metabolism as well as natural biomarkers within the context of their biological functions. The second section outlines the theoretical and technical background of quantitative, noninvasive, autofluorescence microscopy and spectroscopy methods, including experimental design, calibration, pitfalls, and remedies of data acquisition and analysis. The last two sections highlight advances in biomedical and biochemical applications, such as monitoring stem cell differentiation in engineered tissues and diagnosing cancer and ophthalmic diseases quantitatively and noninvasively. Tailored to Interdisciplinary Researchers Covering cell biology, imaging techniques, and clinical diagnostics, this book provides readers with a complete guide to studying cellular/tissue metabolism under healthy, diseased, and environment-induced stress conditions using natural biomarkers. The book is designed for graduate and advanced undergraduate students, biophysics instructors, medical researchers, and those in pharmaceutical R&D.

Time-correlated single photon counting

Author : Desmond O'Connor
Publisher : Academic Press
ISBN 13 : 0323141447
Total Pages : 299 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Time-correlated single photon counting by : Desmond O'Connor

Download or read book Time-correlated single photon counting written by Desmond O'Connor and published by Academic Press. This book was released on 2012-12-02 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-correlated Single Photon Counting has been written in the hope that by relating the authors' experiences with a variety of different single photon counting systems, they may provide a useful service to users and potential users of this formidably sensitive technique. Of all the techniques available to obtain information on the rates of depopulation of excited electronic singlet states of molecular species, monitoring of fluorescence provides, in principle, the simplest and most direct measure of concentration. This volume comprises eight chapters, with the first focusing on the time dependence and applications of fluorescence. Succeeding chapters go on to discuss basic principles of the single photon counting lifetime measurement; light sources; photomultipliers; electronics; data analysis; nanosecond time-resolved emission spectroscopy; time dependence of fluorescence anisotropy. This book will be of interest to practitioners in the field of chemistry.

Breast Cancer

Author : Jian Cao
Publisher : Humana
ISBN 13 : 9781493934423
Total Pages : 0 pages
Book Rating : 4.9/5 (344 download)

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Book Synopsis Breast Cancer by : Jian Cao

Download or read book Breast Cancer written by Jian Cao and published by Humana. This book was released on 2016-01-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides resources, ideas, and bench manuals for the study of breast cancer. This book is divided into five sections: methods used in clinical laboratory for diagnosis (Detection of Molecular Markers of Breast Cancer); methods used in both clinical and research laboratories for testing genetic alterations (Genetic Detection of Breast Cancer) and (Isolation of Breast Cancer Cells); methods used to study the behavior of breast cancer cells (In Vitro Experimental Assays for Breast Cancer); and methods used for mimicking human breast cancer in a living organism (In Vivo Experimental Models for Breast Cancer). Breast Cancer: Methods and Protocols also explores several recently developed techniques for the study of breast cancer progression. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Practical and thorough, Breast Cancer: Methods and Protocols, is a valuable handbook for both graduate and advanced undergraduate students of biological sciences, as well as scientists, technicians, and physicians working in the academic, hospital, or pharmaceutical industry aimed at studying or diagnosing breast cancer.

Advanced Time-Correlated Single Photon Counting Techniques

Author : Wolfgang Becker
Publisher : Springer Science & Business Media
ISBN 13 : 3540288821
Total Pages : 414 pages
Book Rating : 4.5/5 (42 download)

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Book Synopsis Advanced Time-Correlated Single Photon Counting Techniques by : Wolfgang Becker

Download or read book Advanced Time-Correlated Single Photon Counting Techniques written by Wolfgang Becker and published by Springer Science & Business Media. This book was released on 2005-12-19 with total page 414 pages. Available in PDF, EPUB and Kindle. Book excerpt: In 1984 Desmond O’Connor and David Phillips published their comprehensive book „Time-correlated Single Photon Counting“. At that time time-correlated s- gle photon counting, or TCSPC, was used primarily to record fluorescence decay functions of dye solutions in cuvettes. From the beginning, TCSPC was an am- ingly sensitive and accurate technique with excellent time-resolution. However, acquisition times were relatively slow due to the low repetition rate of the light sources and the limited speed of the electronics of the 70s and early 80s. Moreover, TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the wa- form of a periodic light signal. Even with these limitations, it was a wonderful te- nique. More than 20 years have elapsed, and electronics and laser techniques have made impressive progress. The number of transistors on a single chip has approximately doubled every 18 months, resulting in a more than 1,000-fold increase in compl- ity and speed. The repetition rate and power of pulsed light sources have increased by about the same factor.

2021 Frontiers in Physics Editor's pick

Author : Alex Hansen
Publisher : Frontiers Media SA
ISBN 13 : 2889710769
Total Pages : 179 pages
Book Rating : 4.8/5 (897 download)

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Book Synopsis 2021 Frontiers in Physics Editor's pick by : Alex Hansen

Download or read book 2021 Frontiers in Physics Editor's pick written by Alex Hansen and published by Frontiers Media SA. This book was released on 2021-07-22 with total page 179 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Linear and Nonlinear Optical Spectroscopy and Microscopy

Author : Mengtao Sun
Publisher : Springer Nature
ISBN 13 : 9819936373
Total Pages : 125 pages
Book Rating : 4.8/5 (199 download)

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Book Synopsis Linear and Nonlinear Optical Spectroscopy and Microscopy by : Mengtao Sun

Download or read book Linear and Nonlinear Optical Spectroscopy and Microscopy written by Mengtao Sun and published by Springer Nature. This book was released on with total page 125 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Advanced Time-Correlated Single Photon Counting Applications

Author : Wolfgang Becker
Publisher : Springer
ISBN 13 : 3319149296
Total Pages : 642 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Advanced Time-Correlated Single Photon Counting Applications by : Wolfgang Becker

Download or read book Advanced Time-Correlated Single Photon Counting Applications written by Wolfgang Becker and published by Springer. This book was released on 2015-04-13 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.

Biomedical Spectroscopy

Author : Kartha V B
Publisher : CRC Press
ISBN 13 : 1040305725
Total Pages : 525 pages
Book Rating : 4.0/5 (43 download)

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Book Synopsis Biomedical Spectroscopy by : Kartha V B

Download or read book Biomedical Spectroscopy written by Kartha V B and published by CRC Press. This book was released on 2025-02-04 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses biomedical spectroscopy and the applications of spectroscopic techniques in advanced medical technology. Applicable to scientists and medical professionals, the aim of this work is to enable them to work together in this field, so that healthcare facilities can be made routinely available in a cost-effective manner—especially for developing countries which may not be able to afford universal healthcare with present day expensive medical technologies. The subject matter of this book also covers – Instrumentation, Experimental Techniques and Computational Methods Spectroscopy of Animal Models Microspectroscopy for Biomedical Applications Clinical Applications of Optical Spectroscopy Spectroscopy of Human Models Print edition not for sale in South Asia (India, Sri Lanka, Nepal, Bangladesh, Pakistan and Bhutan)

Reflectance Confocal Microscopy for Skin Diseases

Author : Rainer Hofmann-Wellenhof
Publisher : Springer Science & Business Media
ISBN 13 : 3642219969
Total Pages : 484 pages
Book Rating : 4.6/5 (422 download)

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Book Synopsis Reflectance Confocal Microscopy for Skin Diseases by : Rainer Hofmann-Wellenhof

Download or read book Reflectance Confocal Microscopy for Skin Diseases written by Rainer Hofmann-Wellenhof and published by Springer Science & Business Media. This book was released on 2012-03-01 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the use and significance of in vivo reflectance confocal microscopy (RCM) for non-invasive high-resolution imaging of the skin. All of the chapters in this hands-on guide are generously illustrated with numerous confocal images and structured in a reader-friendly way. The contents include detailed information on the most relevant and up-to-date aspects of RCM, schematic drawings summarizing and explaining the most important RCM criteria, and a chapter specifically devoted to bridging the gap between dermoscopy, RCM, and histopathology. At the end of each chapter, core messages recapitulate the most pertinent aspects. Reflectance Confocal Microscopy for Skin Diseases will be a valuable resource for all physicians involved in the diagnosis and treatment of neoplastic and inflammatory skin diseases.

Nanodiamonds in Analytical and Biological Sciences

Author : Wesley Wei-Wen Hsiao
Publisher : John Wiley & Sons
ISBN 13 : 1394202156
Total Pages : 292 pages
Book Rating : 4.3/5 (942 download)

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Book Synopsis Nanodiamonds in Analytical and Biological Sciences by : Wesley Wei-Wen Hsiao

Download or read book Nanodiamonds in Analytical and Biological Sciences written by Wesley Wei-Wen Hsiao and published by John Wiley & Sons. This book was released on 2023-09-19 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: NANODIAMONDS small capIN/small cap ANALYTICAL small capAND/small cap BIOLOGICAL SCIENCES Comprehensive resource highlighting the significance and applications of fluorescent and non-fluorescent nanodiamonds in various domains Nanodiamonds in Analytical and Biological Sciences combines the disciplines of chemistry, physics, materials science, and biology to demonstrate the significance of nanodiamonds, offering precise analysis of the impacts and up-to-date applications of fluorescent and non-fluorescent nanodiamonds, including in COVID-19 and artificial intelligence, with illustrations, case studies, practical examples, and novel perspectives included throughout. Edited by two highly qualified scholars with significant experience in the field, topics covered include: Fundamental properties, synthesis, mechanisms, and functionalization of nanodiamonds, and toxicity assessment Fabrication and surface modification of fluorescent nanodiamonds and in vitro and in vivo bioimaging of fluorescent nanodiamonds Nanodiamond-enabled drug delivery and nanodiamond for mass spectrometry-based analysis of peptides, proteins, and proteomes Quantum sensing, applications in physico-chemical and biomedical sensing, and perspective and outlook on the future of nanodiamond research Nano-scale thermometry of fluorescent nanodiamonds and nanodiamond-enabled drug delivery Offering a balanced, multidisciplinary, and comprehensive overview of the significance of fluorescent and non-fluorescent nanodiamonds, Nanodiamonds in Analytical and Biological Sciences is an essential resource for academic researchers and industry professionals working in fields spanning chemistry, physics, materials science and biology.

Handbook of Optical Sensors

Author : Jose Luis Santos
Publisher : CRC Press
ISBN 13 : 1439866864
Total Pages : 706 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Handbook of Optical Sensors by : Jose Luis Santos

Download or read book Handbook of Optical Sensors written by Jose Luis Santos and published by CRC Press. This book was released on 2014-10-29 with total page 706 pages. Available in PDF, EPUB and Kindle. Book excerpt: Handbook of Optical Sensors provides a comprehensive and integrated view of optical sensors, addressing the fundamentals, structures, technologies, applications, and future perspectives. Featuring chapters authored by recognized experts and major contributors to the field, this essential reference: Explains the basic aspects of optical sensors and