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Multi Scale 3d Virtual Histology Via Phase Contrast X Ray Tomography With Synchrotron Radiation
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Book Synopsis Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation by : Jasper Frohn
Download or read book Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation written by Jasper Frohn and published by Universitätsverlag Göttingen. This book was released on 2023 with total page 148 pages. Available in PDF, EPUB and Kindle. Book excerpt: To this day, the standard method for investigating biological tissue with cellular resolution is the examination under a light microscope, first denoted as histology by Karl Meyer in 1819. Despite the enormous success and importance of histology, it has two major disadvantages. Firstly, the specimen must be physically cut into thin sections due to the limited penetrating power of optical light, and secondly, additional staining of the specimen is required to achieve sufficient image contrast. Both disadvantages can be overcome by the non-destructive method of propagation-based X-ray phase-contrast tomography. While the mechanism of phase-contrast provides sufficient image contrast to image single cells, a tomographic imaging scheme with penetrating X-rays allows for an undamaged sample by virtually slicing the reconstructed 3D sample volume. In this work, the holotomography setup of the synchrotron endstation „GINIX“ (The Göttingen Instrument for Nanoscale-Imaging with X-Rays) was extended to a multi-scale X-ray phase-contrast tomography setup suitable for 3D virtual histology by adding two acquisition schemes. Compared to the existing setup, the first additional scheme is a propagation-based microtomography setup, which enlarges the reconstructed 3D volumes by a factor of approx. 64 at a fraction of the acquisition time (ca. 2 min). The second additional scheme aims for higher resolutions. To this end, the X-ray waveguide illumination was combined with photon counting detector with a large field of view and a novel phase reconstruction scheme, which is based on iterative farfield phase retrieval without an „empty-beam correction“ in the detector plane.
Book Synopsis Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation by : Jasper Frohn
Download or read book Multi-scale 3D Virtual Histology Via Phase-contrast X-ray Tomography with Synchrotron Radiation written by Jasper Frohn and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues by : Marius Reichardt
Download or read book Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues written by Marius Reichardt and published by Universitätsverlag Göttingen. This book was released on 2022 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed.
Book Synopsis 3d virtual histology of neuronal tissue by propagation-based x-ray phase-contrast tomography by : Mareike Töpperwien
Download or read book 3d virtual histology of neuronal tissue by propagation-based x-ray phase-contrast tomography written by Mareike Töpperwien and published by Göttingen University Press. This book was released on 2018 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Deciphering the three-dimensional (3d) cytoarchitecture of neuronal tissue is an important step towards understanding the connection between tissue function and structure and determining relevant changes in neurodegenerative diseases. The gold standard in pathology is histology, in which the tissue is examined under a light microscope after serial sectioning and subsequent staining. It is an invasive and labor-intensive technique which is prone to artifacts due to the slicing procedure. While it provides excellent results on the 2d slices, the 3d anatomy can only be determined after aligning the individual sections, leading to a non-isotropic resolution within the tissue. X-ray computed tomography (CT) offers a promising alternative due to its potential resolution and large penetration depth which allows for non-invasive imaging of the sample's 3d density distribution. In classical CT, contrast formation is based on absorption of the x-rays as they pass through the sample. However, weakly absorbing samples like soft tissue from the central nervous system give nearly no contrast. By exploiting the much stronger phase shifts for contrast formation, which the sample induces in a (partially) coherent wavefront, it can be substantially increased. During free-space propagation behind the sample, these phase shifts are converted to a measurable intensity image by interference of the disturbed wave fronts. In this thesis, 3d virtual histology is performed by means of propagation-based x-ray phase-contrast tomography on tissue from the central nervous system of humans and mice. A combination of synchrotron-based and laboratory setups is used to visualize the 3d density distribution on varying lengths scales from the whole organ down to single cells. By comparing and optimizing different preparation techniques and phase-retrieval approaches, even sub-cellular resolution can be reached in mm-sized tissue blocks. The development of an automatic cell segmentation workflow provides access to the 3d cellular distribution within the tissue, enabling the quantification of the cellular arrangement and allowing for extensive statistical analysis based on several thousands to millions of cells. This paves the way for biomedical studies aimed at changes in cellular distribution, e.g., in the course of neurodegenerative diseases such as multiple sclerosis, Alzheimer's disease or ischemic stroke.
Book Synopsis Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues by : Marius Reichardt
Download or read book Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues written by Marius Reichardt and published by . This book was released on 2022 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence. However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy. This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure. While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction, the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed.
Book Synopsis Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues by :
Download or read book Multiscale X-ray Structural Analysis of Cardiac Cells and Tissues written by and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The cardiac function relies on an intricate molecular and cellular three-dimensional (3d) architecture of a complex, dense and co-dependent cellular network. Structural alterations of the cardiac structure can affect its essential function and lead to severe dysfunction of the organ. Cardiovascular diseases are the main cause of death worldwide with a rising incidence.However, it is not possible to give a generalized answer how the heart is formed. Up to now, cardiac structure as well as physiologic and disease-related tissue alterations of the tissue are mainly investigated by established 2d imaging methods such as optical microscopy or electron microscopy.This work presents a multiscale and multimodal X-ray imaging approach, which allows to probe the heart structure from the scale of entire intact murine hearts to the molecular organisation of the sarcomer structure.While the molecular structure of the actomyosin complex is probed by scanning X-ray diffraction,the 3d arrangement of the cellular network is investigated by propagation-based X-ray phase-contrast tomography. In this context, the concept of 3d virtual histology of cardiac tissue by X-ray phase-contrast tomography using laboratory sources as well as highly coherent synchrotron radiation is being further developed.
Book Synopsis Novel approaches in cardiac imaging by : Giuseppe Pannarale
Download or read book Novel approaches in cardiac imaging written by Giuseppe Pannarale and published by Frontiers Media SA. This book was released on 2023-06-30 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Cone-beam x-ray phase-contrast tomography for the observation of single cells in whole organs by : Martin Krenkel
Download or read book Cone-beam x-ray phase-contrast tomography for the observation of single cells in whole organs written by Martin Krenkel and published by Göttingen University Press. This book was released on 2015 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray imaging enables the nondestructive investigation of interior structures in otherwise opaque samples. In particular the use of computed tomography (CT) allows for arbitrary virtual slices through the object and 3D information about intricate structures can be obtained. However, when it comes to image very small structures like single cells, the classical CT approach is limited by the weak absorption of soft-tissue. The use of phase information, encoded in measureable intensity images by free-space propagation of coherent x-rays, allows a huge increase in contrast, which enables 3D reconstructions at higher resolutions. In this work the application of propagation-based phase-contrast tomography to lung tissue samples is demonstrated in close to in vivo conditions. Reconstructions of the lung structure of whole mice at down to 5 μm resolution are obtained at a selfbuilt CT setup, which is based on a liquid-metal jet x-ray source. To reach even higher resolutions, synchrotron radiation in combination with suitable holographic phase-retrieval algorithms is employed. Due to optimized cone-beam geometry, field of view and resolution can be varied over a wide range of parameters, so that information on different length scales can be achieved, covering several millimeters field of view down to a 3D resolution of 50 nm. Thus, the sub-cellular 3D imaging of single cells embedded in large pieces of tissue is enabled, which paves the way for future biomedical research.
Book Synopsis Advancing the Characterization of Neuronal Cyto-Architecture by X-ray Phase-Contrast Tomography by : Marina Eckermann
Download or read book Advancing the Characterization of Neuronal Cyto-Architecture by X-ray Phase-Contrast Tomography written by Marina Eckermann and published by Universitätsverlag Göttingen. This book was released on 2021 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt: To bring physiology and pathology of the human brain into better micro-anatomical and histological context, studies with different methodologies are required. Established techniques such as electron microscopy or histology show limitations in view of invasiveness, labor-intense and artifact-prone sample preparation, as well as an adequate ratio between resolution and volume throughput. For this reason, X-ray phase-contrast tomography (PC-CT) has been proposed as a three-dimensional non-destructive imaging technique, which requires less effort in sample preparation and can assess larger volumes. Furthermore, it offers quantitative electron density based contrast even for unstained tissue. Up to now, however, PC-CT studies fell short in number of samples, so that structural alterations caused by neurodegenerative diseases cannot be distinguished from physiological inter-subject variations. In this thesis, the scalability of PC-CT with respect to the required number of samples and resolution-to-volume-throughput is demonstrated, and the methodology is advanced with respect to data acquisition, processing and segmentation. In addition to the human cerebellum, cortex and hippocampus are studied. Concerning quantification and analysis of PC-CT data, this work introduces optimal transport analysis to obtain quantitative metrics of the cyto-architecture and to identify changes due to neurodegenerative diseases. For the case of Alzheimer’s disease, this workflow reveals a yet undescribed compactification of granular cells in the human hippocampus. This thesis also provides optimized configurations to study neural tissues with laboratory instrumentation, and – finally – provides new correlative imaging approaches, in particular with scanning electron microscopy.
Book Synopsis Vertebrate Skeletal Histology and Paleohistology by : Vivian de Buffrénil
Download or read book Vertebrate Skeletal Histology and Paleohistology written by Vivian de Buffrénil and published by CRC Press. This book was released on 2021-06-24 with total page 838 pages. Available in PDF, EPUB and Kindle. Book excerpt: Vertebrate Skeletal Histology and Paleohistology summarizes decades of research into the biology and biological meaning of hard tissues, in both living and extinct vertebrates. In addition to outlining anatomical diversity, it provides fundamental phylogenetic and evolutionary contexts for interpretation. An international team of leading authorities review the impact of ontogeny, mechanics, and environment in relation to bone and dental tissues. Synthesizing current advances in the biological problems of growth, metabolism, evolution, ecology, and behavior, this comprehensive and authoritative volume is built upon a foundation of concepts and technology generated over the past fifty years.
Book Synopsis Cone-beam X-ray Phase Contrast Tomography of Biological Samples by : Matthias Bartels
Download or read book Cone-beam X-ray Phase Contrast Tomography of Biological Samples written by Matthias Bartels and published by Universitätsverlag Göttingen. This book was released on 2013 with total page 220 pages. Available in PDF, EPUB and Kindle. Book excerpt: Three-dimensional information of entire objects can be obtained by the remarkable technique of computed tomography (CT). In combination with phase sensitive X-ray imaging high contrast for soft tissue structures can be achieved as opposed to CT based on classical radiography. In this work biological samples ranging from micrometer sized single cells over multi-cellular nerve tissue to entire millimeter sized organs are investigated by use of cone-beam propagationbased X-ray phase contrast. Optimization with respect to contrast, resolution and field of view is achieved by addressing instrumentation, sample preparation and phase reconstruction techniques. By using laboratory sources functional soft tissue within the bony capsule of mouse cochleae is visualized in 3D with unprecedented image quality. At synchrotron storage rings the technique reveals more than 1000 axons running in parallel within a mouse nerve and enables doseefficient three-dimensional cellular imaging as well as two-dimensional imaging at high resolutions below 50 nm.
Book Synopsis Development and Application of the Analyzer-based Imaging Technique with Hard Synchrotron Radiation by : Paola Coan
Download or read book Development and Application of the Analyzer-based Imaging Technique with Hard Synchrotron Radiation written by Paola Coan and published by . This book was released on 2006 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: THE OBJECTIVE OF THIS THESIS IS TWOFOLD: FROM ONE SI DE THE APPLICATION OF THE ANALYSER-BASED X-RAY PHASE CONTRAST IMAGING TO STUDY CARTILAGE, BONE AND BONE IMPLANTS USING THE 1017 AND 1019 ESRF SYNCHROTRON RADIATION SOURCES AND ON THE OTHER TO CONTRIBUTE TO THE DEVELOPMENT OF THE PHASE CONTRAST TECHNIQUES FROM THE THEORETICAL AND EXPERIMENTAL POINT OF VIEW. SEVERAL HUMAN SAMPLES HAVE BEEN STUDIED lN VITRO USING THE ANALYSER BASED IMAGING (ABI) TECHNIQUE. EXAMINATION INCLUDED PROJECTION AND COMPUTED TOMOGRAPHY IMAGING AND 3D VOLUME RENDERING OF HIP, BIG TOE AND ANKLE ARTICULAR JOINTS. X-RAY ABI IMAGES HAVE BEEN CRITICALLY COMPARED WITH THOSE OBTAINED WITH THE CONVENTIONAL TECHNIQUES, INCLUDING RADIOGRAPHY, COMPUTED TOMOGRAPHY, ULTRASOUND, MAGNETIC RESONANCE AND HISTOLOGY, THE LATTER TAKEN AS GOLO STANDARD. RESUL TS SHOW THAT ONL Y ABI IMAGING WAS ABLE TO EITHER VISUALIZE OR CORRECTLY ESTIMATE THE EARLY PATHOLOGICAL STATUS OF THE CARTILAGE. THE STATUS OF THE BONE INGROWTH lN SHEEP IMPLANTS HAVE ALSO BEEN EXAMINED lN VITRO: ABI IMAGES PERMITTED TO CORRECTL Y DISTINGUISH BETWEEN GOOD AND INCOMPLETE BONE HEALING. PIONEERING INVIVO ABlON GUINEA PIGS WERE ALSO SUCCESSFULL Y PERFORMED, CONFIRMING THE POSSIBLE USE OF THE TECHNIQUE TO FOLLOW UP THE PROGRESSION OF JOINT DISEASES, THE BONE/METAL INGROWTH AND THE EFFICACY OF DRUGS TREATMENTS. AS PART OF THE DEVELOPMENT OF THE PHASE CONTRAST TECHNIQUES, TWO OBJECTIVES HAVE BEEN REACHED. FIRSTL Y, IT HAS BEEN EXPERIMENTALL Y DEMONSTRATED FOR THE FIRST TIME THAT THE ABI AND THE PROPAGATION BASED IMAGING (PBI) CAN BE COMBINED TO CREATE IMAGES WITH ORIGINAL FEATURES (HYBRID IMAGING, HI). SECONDL Y, IT HAS BEEN PROPOSED AND EXPERIMENTALL Y TESTED A NEW SIMPLIFIED SET-UP CAPABLE TO PRODUCE IMAGES WITH PROPERTIES SIMILAR TO THOSE OBTAINED WITH THE ABI TECHNIQUE OR HI. FINALL Y, BOTH THE ABI AND THE HI HAVE BEEN THEORETICAL STUDIED WITH AN INNOVATIVE, WAVE-BASED SIMULATION PROGRAM, WHICH WAS ABLE TO CORRECTL Y REPRODUCE EXPERIMENTAL RESUL TS.
Book Synopsis Computed Tomography by : Willi A. Kalender
Download or read book Computed Tomography written by Willi A. Kalender and published by John Wiley & Sons. This book was released on 2011-07-07 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book offers a comprehensive and user-oriented description of the theoretical and technical system fundamentals of computed tomography (CT) for a wide readership, from conventional single-slice acquisitions to volume acquisition with multi-slice and cone-beam spiral CT. It covers in detail all characteristic parameters relevant for image quality and all performance features significant for clinical application. Readers will thus be informed how to use a CT system to an optimum depending on the different diagnostic requirements. This includes a detailed discussion about the dose required and about dose measurements as well as how to reduce dose in CT. All considerations pay special attention to spiral CT and to new developments towards advanced multi-slice and cone-beam CT. For the third edition most of the contents have been updated and latest topics like dual source CT, dual energy CT, flat detector CT and interventional CT have been added. The enclosed CD-ROM again offers copies of all figures in the book and attractive case studies, including many examples from the most recent 64-slice acquisitions, and interactive exercises for image viewing and manipulation. This book is intended for all those who work daily, regularly or even only occasionally with CT: physicians, radiographers, engineers, technicians and physicists. A glossary describes all the important technical terms in alphabetical order. The enclosed DVD again offers attractive case studies, including many examples from the most recent 64-slice acquisitions, and interactive exercises for image viewing and manipulation. This book is intended for all those who work daily, regularly or even only occasionally with CT: physicians, radiographers, engineers, technicians and physicists. A glossary describes all the important technical terms in alphabetical order.
Book Synopsis Nanoscale Photonic Imaging by : Tim Salditt
Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.
Book Synopsis Coherent X-Ray Optics by : David Paganin
Download or read book Coherent X-Ray Optics written by David Paganin and published by Oxford University Press on Demand. This book was released on 2006-01-12 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray optics is undergoing a renaissance, which may be paralleled to that experienced by visible-light optics following the invention of the laser. The associated surge of activity in "coherent" x-ray optics has been documented in this monograph, the first of its type in the field.
Book Synopsis Fish as Model Organism for Skeletal Diseases by : Erika Kague
Download or read book Fish as Model Organism for Skeletal Diseases written by Erika Kague and published by Frontiers Media SA. This book was released on 2023-11-30 with total page 224 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis X-ray Phase-Contrast Imaging Using Near-Field Speckles by : Marie-Christine Zdora
Download or read book X-ray Phase-Contrast Imaging Using Near-Field Speckles written by Marie-Christine Zdora and published by Springer Nature. This book was released on 2021-02-16 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents research on novel X-ray imaging methods that improve the study of specimens with small density differences, revealing their inner structure and density distribution. Exploiting the phase shift of X-rays in a material can significantly increase the image contrast compared to conventional absorption imaging. This thesis provides a practical guide to X-ray phase-contrast imaging with a strong focus on X-ray speckle-based imaging, the most recently developed phase-sensitive method. X-ray speckle-based imaging only requires a piece of abrasive paper in addition to the standard X-ray imaging setup. Its simplicity and robustness combined with the compatibility with laboratory X-ray sources, make it an ideal candidate for wide user uptake in a range of fields. An in-depth overview of the state of the art of X-ray speckle-based imaging and its latest developments is given in this thesis. It, furthermore, explores a broad range of applications, from X-ray optics characterisation, to biomedical imaging for 3D virtual histology and geological studies of volcanic rocks, demonstrating is promising potential. Moreover, the speckle-based technique is placed in the context of other phase-sensitive X-ray imaging methods to assist in the choice of a suitable method, hence serving as a guide and reference work for future users.
