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Monitoring Hormone And Neurotransmitters By Capillary Electrophoresis With Laser Induced Fluorescence Detection
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Book Synopsis Monitoring Hormone and Neurotransmitters by Capillary Electrophoresis with Laser Induced Fluorescence Detection by : Li Tao
Download or read book Monitoring Hormone and Neurotransmitters by Capillary Electrophoresis with Laser Induced Fluorescence Detection written by Li Tao and published by . This book was released on 1998 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Amino Acid Neurotransmitters in Cerebrospinal Fluid by : Murali Seenappa
Download or read book Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Amino Acid Neurotransmitters in Cerebrospinal Fluid written by Murali Seenappa and published by . This book was released on 2004 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Direct Methods for Dynamic Monitoring of Secretions from Single Cells by Capillary Electrophoresis and Microscopy with Laser-induced Native Fluorescence Detection by :
Download or read book Direct Methods for Dynamic Monitoring of Secretions from Single Cells by Capillary Electrophoresis and Microscopy with Laser-induced Native Fluorescence Detection written by and published by . This book was released on 1997 with total page 27 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microscale separation and detection methods for real-time monitoring of dynamic cellular processes (e.g., secretion) by capillary electrophoresis (CE) and microscopic imaging were developed. Ultraviolet laser-induced native fluorescence (LINF) provides simple, sensitive and direct detection of neurotransmitters and proteins without any derivatization. An on-column CE-LINF protocol for quantification of the release from single cell was demonstrated. Quantitative measurements of both the amount of insulin released from and the amount remaining in the cell ([beta]TC3) were achieved simultaneously. Secretion of catecholamines (norepinephrine (NE) and epinephrine (E)) from individual bovine adrenal chromaffin cells was determined using the on-column CE-LINF. Direct visualization of the secretion process of individual bovine adrenal chromaffin cells was achieved by LINF imaging microscopy with high temporal and spatial resolution. The secretion of serotonin from individual leech Retzius neurons was directly characterized by LINF microscopy with high spatial resolution.
Book Synopsis Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Tara Lee Massie
Download or read book Analysis by Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Tara Lee Massie and published by . This book was released on 2010 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis with laser-induced fluorescence detection (CE-LIF) is a versatile and sensitive analytical tool with many potential applications. Its advantages over other chromatographic methods include minimal solvent and sample requirements, low waste volumes, simple instrumentation, and diversity of analytes studied. CE is one of the most efficient separation techniques available for the analysis of both large and small molecules, but because few analytes are natively fluorescent, it is necessary to bind them with fluorescent tags if LIF detection is to be employed. These tags increase sensitivity and provide additional separation from components of the sample that do not bind the tags. In this dissertation, four applications of CE-LIF for the determination of tagged analytes are documented. First, a rapid CE-LIF method was developed for the determination of gamma-aminobutyric acid (GABA) and other important brain biogenic amines and amino acids present in biological samples at low levels. The high efficiency and sensitivity necessary to identify toxicologically relevant changes in neurotransmitter levels in brain tissue and microdialysates following Mn exposure was achieved, and the method required minimum sample work-up and quantities of sample for analysis. A linear relationship between the concentration of GABA and the CE-LIF peak height was established, with correlation coefficients of 0.989 or better for four neurotransmitters. Second, the use of two squarylium-based fluorescent dyes (bis-SQHN-4d and SQHN-3c) as noncovalent protein labels was investigated. The fluorescence emissions of both dyes were enhanced upon complexation with model proteins, demonstrating promise as noncovalent fluorescent protein labels. Third, the nature of interactions between two boronic acid-based probes (SQTM-BA1 and ANQW-BA1) and two model proteins (bovine serum albumin (BSA) and hemagglutinin (HA)) have been explored. The complex between SQTM-BA1 and BSA was the only complex to show increased fluorescence emission, and the role of electrostatic interactions between SQTM-BA1 and BSA in complex formation was explored. Lastly, CE-LIF methods were developed to label and quantify turnip yellow mosaic virus (TYMV). Noncovalent labels Red-1c and NN127 effectively labeled the surface proteins of the virus, and the labeled viruses were then analyzed by CE-LIF. The CE-LIF methods developed herein for the analysis of neurotransmitters, free solution proteins, and viruses demonstrate the versatility of the technique, especially when coupled with fluorescent labeling.
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by :
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by and published by . This book was released on 1995 with total page 52 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Ph. D. Thesis describes several separation and detection schemes for the analysis of small volume and amount of samples, such as intracellular components and single enzymes developed during research. Indirect Laser-induced fluorescence detection and capillary electrophoresis were used to quantify lactate and pyruvate in single red blood cells. The assay of specific enzyme activities was achieved by monitoring the highly fluorescent enzymatic reaction product, NADH. LDH activity was found not to be a unique marker for diagnosis of leukemia. Reactions of single LDH-1 molecules were investigated by monitoring the reaction product with LIF detection.
Book Synopsis Direct Methods for Dynamic Monitoring of Secretions from Single Cells by Capillary Electrophoresis and Microscopy with Laser-induced Native Fluorescence Detection by : Wei Tong
Download or read book Direct Methods for Dynamic Monitoring of Secretions from Single Cells by Capillary Electrophoresis and Microscopy with Laser-induced Native Fluorescence Detection written by Wei Tong and published by . This book was released on 1997 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: The results from images of co-cultured pressure sensory (P) (non-serotonergic) and Retzius (serotonergic) cells, as well as capillary electrophoresis, suggested that the major native fluorescence excited by the laser was derived from intracellular serotonin. A much more substantial release of serotonin was found at the axon stump left during cell isolation, a preferred region for synapse formation. The release of serotonin was also characterized to be calcium dependent.
Book Synopsis Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species by : Qifeng Xue
Download or read book Applications of Capillary Electrophoresis and Laser-induced Fluorescence Detection to the Analysis of Trace Species written by Qifeng Xue and published by . This book was released on 1995 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides by : Guido Fridolin Verbeck
Download or read book Two-wavelength Laser Induced Fluorescence Detection for Capillary Electrophoresis of Proteins and Peptides written by Guido Fridolin Verbeck and published by . This book was released on 1999 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis/laser-induced Fluorescence Detection of Gamma-aminobutyric Acid in Subnanoliter Sample Volumes by : Louise Clare Pyle
Download or read book Capillary Electrophoresis/laser-induced Fluorescence Detection of Gamma-aminobutyric Acid in Subnanoliter Sample Volumes written by Louise Clare Pyle and published by . This book was released on 2000 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis APPLICATION OF CAPILLARY ELECT by : 易秀麗
Download or read book APPLICATION OF CAPILLARY ELECT written by 易秀麗 and published by Open Dissertation Press. This book was released on 2017-01-27 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "The Application of Capillary Electrophoresis With Laser-induced Fluorescence Detection in Quantifying the Endogenous Amino Acid Pool of Mouse Embryos" by 易秀麗, Sau-lai, Yik, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. DOI: 10.5353/th_b3122383 Subjects: Capillary electrophoresis Fluorescence spectroscopy Amino acids Mice - Embryos
Book Synopsis Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Glucose in Biological Samples by : Sherryl A. Perez
Download or read book Capillary Electrophoresis with Laser-induced Fluorescence Detection for the Determination of Glucose in Biological Samples written by Sherryl A. Perez and published by . This book was released on 1995 with total page 132 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Ultrasensitive Analysis of Single Neurons Using Capillary Electrophoresis with Laser Induced Fluorescence by : David C. Essaka
Download or read book Ultrasensitive Analysis of Single Neurons Using Capillary Electrophoresis with Laser Induced Fluorescence written by David C. Essaka and published by . This book was released on 2012 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: A new method for the ultrasensitive analysis of single cells has been established, which involves the separation of the cellular content in a capillary, one cell at the time, and detection of any fluorescently tagged analytes by laser induced fluorescence. The present work focuses on instrumentation development and validation, which further demonstrates the applicability of our technology in problems with biological relevance. First, the linear dynamic range of the analysis by capillary electrophoresis with laser induced fluorescence (CE-LIF) was improved to an unprecedented nine orders of magnitude; this was done with the use of beam-splitters and detectors added into our existing technology. Using a lipid substrate GM1 labeled with tetramethylrhodamine (TMR), the upgraded instrument demonstrated that only the catabolic compartments of the cell were probed in neurons from the rat cerebellum. New strategies for probing anabolism were evaluated, including the use of Bodipy-labeled substrates, which did allow probing the anabolic compartments within the cell. This lead to our second instrumentation upgrade: the design of a CE-LIF system capable of two-color detection, which enabled the simultaneous analysis of concurrently occurring degradative and biosynthetic pathways within a single neuron, while maintaining the sensitivity that is the hallmark of this technology with zeptomole (10−21) to yoctomole (10−24) limits of detection.
Book Synopsis Capillary Electrophoresis with Laser-induced Fluorescence Detection as a Tool for Glycosyltransferase Characterization by :
Download or read book Capillary Electrophoresis with Laser-induced Fluorescence Detection as a Tool for Glycosyltransferase Characterization written by and published by . This book was released on 1999 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis with Post-column Laser-induced Fluorescence Detection of Proteins by :
Download or read book Capillary Electrophoresis with Post-column Laser-induced Fluorescence Detection of Proteins written by and published by . This book was released on 1996 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis with laser induced fluorescence detection (CE-LIF) is one of the alternative methods being developed for fast and sensitive assays of proteins and peptides. This report summarizes CE-LIF instrument development, efforts at labelling proteins, tests conducted to prove the applicability of CE-LIF to the analysis of samples, and results of a comparison of CE-LIF with some other techniques. Appendices include an instrument operating procedure and a paper that summarizes the experimental details of the protein labelling procedure and a CE-LIF technique for sub-nanomolar assay of proteins.
Book Synopsis Preconcentration Techniques in Capillary Electrophoresis with Laser-induced Fluorescence Detection by : Jiang Jiang
Download or read book Preconcentration Techniques in Capillary Electrophoresis with Laser-induced Fluorescence Detection written by Jiang Jiang and published by . This book was released on 2005 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis An Investigation on Capillary Electrophoresis-based Immunoassay with Laser Induced Fluorescence Detection by : Jianping Ou
Download or read book An Investigation on Capillary Electrophoresis-based Immunoassay with Laser Induced Fluorescence Detection written by Jianping Ou and published by . This book was released on 1999 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection by : Sheri Joanne Lillard
Download or read book Single Cell Analysis by Capillary Electrophoresis with Laser-induced Native Fluorescence Detection written by Sheri Joanne Lillard and published by . This book was released on 1996 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
