Molecular Genetic Approaches to Maize Improvement

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Publisher : Springer Science & Business Media
ISBN 13 : 3540689222
Total Pages : 370 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis Molecular Genetic Approaches to Maize Improvement by : Alan L. Kriz

Download or read book Molecular Genetic Approaches to Maize Improvement written by Alan L. Kriz and published by Springer Science & Business Media. This book was released on 2008-11-14 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, there has been tremendous progress in maize biotechnology. This volume provides an overview of our current knowledge of maize molecular genetics, how it is being used to improve the crop, and future possibilities for crop enhancement. Several chapters deal with genetically engineered traits that are currently, or soon will be, in commercial production. Technical approaches for introducing novel genes into the maize genome, the regeneration of plants from transformed cells, and the creation of transgenic lines for field production are covered. Further, the authors describe how molecular genetic techniques are being used to identify genes and characterize their function, and how these procedures are utilized to develop elite maize germplasm. Moreover, molecular biology and physiological studies of corn as a basis for the improvement of its nutritional and food-making properties are included. Finally, the growing use of corn as biomass for energy production is discussed.

Molecular Genetic Approaches to Maize Improvement

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Publisher : Springer
ISBN 13 : 9783540689195
Total Pages : 369 pages
Book Rating : 4.6/5 (891 download)

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Book Synopsis Molecular Genetic Approaches to Maize Improvement by : Alan L. Kriz

Download or read book Molecular Genetic Approaches to Maize Improvement written by Alan L. Kriz and published by Springer. This book was released on 2008-11-04 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, there has been tremendous progress in maize biotechnology. This volume provides an overview of our current knowledge of maize molecular genetics, how it is being used to improve the crop, and future possibilities for crop enhancement. Several chapters deal with genetically engineered traits that are currently, or soon will be, in commercial production. Technical approaches for introducing novel genes into the maize genome, the regeneration of plants from transformed cells, and the creation of transgenic lines for field production are covered. Further, the authors describe how molecular genetic techniques are being used to identify genes and characterize their function, and how these procedures are utilized to develop elite maize germplasm. Moreover, molecular biology and physiological studies of corn as a basis for the improvement of its nutritional and food-making properties are included. Finally, the growing use of corn as biomass for energy production is discussed.

Maize Germplasm

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Author :
Publisher : BoD – Books on Demand
ISBN 13 : 1789230381
Total Pages : 102 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Maize Germplasm by : Mohamed A. El-Esawi

Download or read book Maize Germplasm written by Mohamed A. El-Esawi and published by BoD – Books on Demand. This book was released on 2018-05-09 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is an important staple food crop worldwide. It is the third most important cereal crop after wheat and rice and is economically used for both livestock feeds and human consumption. The latest maize research has opened up new opportunities for crop improvement. This book brings together recent work and advances that have recently been made in the dynamic fields of genetic characterization, molecular breeding, genetic engineering technologies, and mapping of agronomic traits of global maize germplasm. It also provides new insights into and sheds new light regarding the current research trends and future research directions in maize. This book will provoke interest in many readers, researchers, and scientists, who can find this information useful for the advancement of their research works toward maize improvement.

Maize Germplasm - Characterization and Genetic Approaches for Crop Improvement

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Publisher :
ISBN 13 : 9781789230390
Total Pages : 100 pages
Book Rating : 4.2/5 (33 download)

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Book Synopsis Maize Germplasm - Characterization and Genetic Approaches for Crop Improvement by : Mohamed El-Esawi

Download or read book Maize Germplasm - Characterization and Genetic Approaches for Crop Improvement written by Mohamed El-Esawi and published by . This book was released on 2018 with total page 100 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is an important staple food crop worldwide. It is the third most important cereal crop after wheat and rice and is economically used for both livestock feeds and human consumption. The latest maize research has opened up new opportunities for crop improvement. This book brings together recent work and advances that have recently been made in the dynamic fields of genetic characterization, molecular breeding, genetic engineering technologies, and mapping of agronomic traits of global maize germplasm. It also provides new insights into and sheds new light regarding the current research trends and future research directions in maize. This book will provoke interest in many readers, researchers, and scientists, who can find this information useful for the advancement of their research works toward maize improvement.

Genetics, Genomics and Breeding of Maize

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Author :
Publisher : CRC Press
ISBN 13 : 1482228130
Total Pages : 310 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Genetics, Genomics and Breeding of Maize by : Ramakrishna Wusirika

Download or read book Genetics, Genomics and Breeding of Maize written by Ramakrishna Wusirika and published by CRC Press. This book was released on 2014-08-05 with total page 310 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sequencing of the maize genome has opened up new opportunities in maize breeding, genetics and genomics research. This book highlights modern trends in development of hybrids, analysis of genetic diversity, molecular breeding, comparative and functional genomics, epigenomicsand proteomics in maize. The use of maize in biofuels, phytoremediation and

Maize and wheat research for the next millennium: A preview of CIMMYT's role

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Author :
Publisher : CIMMYT
ISBN 13 : 9789686923674
Total Pages : 16 pages
Book Rating : 4.9/5 (236 download)

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Book Synopsis Maize and wheat research for the next millennium: A preview of CIMMYT's role by : International Maize and Wheat Improvement Center

Download or read book Maize and wheat research for the next millennium: A preview of CIMMYT's role written by International Maize and Wheat Improvement Center and published by CIMMYT. This book was released on 1996* with total page 16 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Doubled Haploid Production in Crop Plants

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Publisher : Springer Science & Business Media
ISBN 13 : 940171293X
Total Pages : 451 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Doubled Haploid Production in Crop Plants by : M. Maluszynski

Download or read book Doubled Haploid Production in Crop Plants written by M. Maluszynski and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 451 pages. Available in PDF, EPUB and Kindle. Book excerpt: The production of doubled haploids has become a necessary tool in advanced plant breeding institutes and commercial companies for breeding many crop species. However, the development of new, more efficient and cheaper large scale production protocols has meant that doubled haploids are also recently being applied in less advanced breeding programmes. This Manual was prepared to stimulate the wider use of this technology for speeding and opening up new breeding possibilities for many crops including some woody tree species. Since the construction of genetic maps using molecular markers requires the development of segregating doubled haploid populations in numerous crop species, we hope that this Manual will also help molecular biologists in establishing such mapping populations. For many years, both the Food and Agriculture Organization of the United Nations (FAO) and the International Atomic Energy Agency (IAEA) have supported and coordinated research that focuses on development of more efficient doubled haploid production methods and their applications in breeding of new varieties and basic research through their Plant Breeding and Genetics Section of the Joint F AO/IAEA Division of Nuclear Techniques in Food and Agriculture. The first F AO/IAEA scientific network (Coordinated Research Programme - CRP) dealing with doubled haploids was initiated by the Plant Breeding and Genetics Section in 1986.

Molecular Approaches for the Genetic Improvement of Cereals for Stable Production in Water-limited Environments

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Publisher : CIMMYT
ISBN 13 : 9706480528
Total Pages : 180 pages
Book Rating : 4.7/5 (64 download)

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Book Synopsis Molecular Approaches for the Genetic Improvement of Cereals for Stable Production in Water-limited Environments by : Jean-Marcel Ribaut

Download or read book Molecular Approaches for the Genetic Improvement of Cereals for Stable Production in Water-limited Environments written by Jean-Marcel Ribaut and published by CIMMYT. This book was released on 2000 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Molecular Approaches to Crop Improvement

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Publisher : Springer Science & Business Media
ISBN 13 : 3709191084
Total Pages : 176 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis Molecular Approaches to Crop Improvement by : Elizabeth S. Dennis

Download or read book Molecular Approaches to Crop Improvement written by Elizabeth S. Dennis and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although plant genes were first isolated only some twelve years ago and transfer of foreign DNA into tobacco cells first demonstrated some eight years ago, the application and extension of biotechnology to agricultural problems has already led to the field-testing of genetically modified crop plants. The promise of tailor-made plants containing resistance to pests or diseases as well as many other desirable characteristics has led to the almost compulsory incorporation of molecular biology into the research programs of chemical and seed companies as well as Governmental agricultural agencies. With the routine transformation of rice and the early evidence of transformation of maize the possibility of the world's major cereal crops being modified for improved nutritional value or resistance characteristics is now likely in the next few years. The increasing number of cloned plant genes and the increasing sophistication of our knowledge of the major developmental and biochemi cal pathways in plants should eventually allow us to engineer crop plants with higher yields and with less detrimental impact on the environment than now occurs in our current high input agricultural systems. This book draws together many of the expanding areas of plant molecular biology and genetic engineering that will make a substantial contribution to the development of the more productive and efficient crop plants that the world's farmers will be planting in the next decade.

Quantitative Genetics in Maize Breeding

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Publisher : Springer Science & Business Media
ISBN 13 : 1441907661
Total Pages : 669 pages
Book Rating : 4.4/5 (419 download)

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Book Synopsis Quantitative Genetics in Maize Breeding by : Arnel R. Hallauer

Download or read book Quantitative Genetics in Maize Breeding written by Arnel R. Hallauer and published by Springer Science & Business Media. This book was released on 2010-09-28 with total page 669 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is used in an endless list of products that are directly or indirectly related to human nutrition and food security. Maize is grown in producer farms, farmers depend on genetically improved cultivars, and maize breeders develop improved maize cultivars for farmers. Nikolai I. Vavilov defined plant breeding as plant evolution directed by man. Among crops, maize is one of the most successful examples for breeder-directed evolution. Maize is a cross-pollinated species with unique and separate male and female organs allowing techniques from both self and cross-pollinated crops to be utilized. As a consequence, a diverse set of breeding methods can be utilized for the development of various maize cultivar types for all economic conditions (e.g., improved populations, inbred lines, and their hybrids for different types of markets). Maize breeding is the science of maize cultivar development. Public investment in maize breeding from 1865 to 1996 was $3 billion (Crosbie et al., 2004) and the return on investment was $260 billion as a consequence of applied maize breeding, even without full understanding of the genetic basis of heterosis. The principles of quantitative genetics have been successfully applied by maize breeders worldwide to adapt and improve germplasm sources of cultivars for very simple traits (e.g. maize flowering) and very complex ones (e.g., grain yield). For instance, genomic efforts have isolated early-maturing genes and QTL for potential MAS but very simple and low cost phenotypic efforts have caused significant and fast genetic progress across genotypes moving elite tropical and late temperate maize northward with minimal investment. Quantitative genetics has allowed the integration of pre-breeding with cultivar development by characterizing populations genetically, adapting them to places never thought of (e.g., tropical to short-seasons), improving them by all sorts of intra- and inter-population recurrent selection methods, extracting lines with more probability of success, and exploiting inbreeding and heterosis. Quantitative genetics in maize breeding has improved the odds of developing outstanding maize cultivars from genetically broad based improved populations such as B73. The inbred-hybrid concept in maize was a public sector invention 100 years ago and it is still considered one of the greatest achievements in plant breeding. Maize hybrids grown by farmers today are still produced following this methodology and there is still no limit to genetic improvement when most genes are targeted in the breeding process. Heterotic effects are unique for each hybrid and exotic genetic materials (e.g., tropical, early maturing) carry useful alleles for complex traits not present in the B73 genome just sequenced while increasing the genetic diversity of U.S. hybrids. Breeding programs based on classical quantitative genetics and selection methods will be the basis for proving theoretical approaches on breeding plans based on molecular markers. Mating designs still offer large sample sizes when compared to QTL approaches and there is still a need to successful integration of these methods. There is a need to increase the genetic diversity of maize hybrids available in the market (e.g., there is a need to increase the number of early maturing testers in the northern U.S.). Public programs can still develop new and genetically diverse products not available in industry. However, public U.S. maize breeding programs have either been discontinued or are eroding because of decreasing state and federal funding toward basic science. Future significant genetic gains in maize are dependent on the incorporation of useful and unique genetic diversity not available in industry (e.g., NDSU EarlyGEM lines). The integration of pre-breeding methods with cultivar development should enhance future breeding efforts to maintain active public breeding programs not only adapting and improving genetically broad-based germplasm but also developing unique products and training the next generation of maize breeders producing research dissertations directly linked to breeding programs. This is especially important in areas where commercial hybrids are not locally bred. More than ever public and private institutions are encouraged to cooperate in order to share breeding rights, research goals, winter nurseries, managed stress environments, and latest technology for the benefit of producing the best possible hybrids for farmers with the least cost. We have the opportunity to link both classical and modern technology for the benefit of breeding in close cooperation with industry without the need for investing in academic labs and time (e.g., industry labs take a week vs months/years in academic labs for the same work). This volume, as part of the Handbook of Plant Breeding series, aims to increase awareness of the relative value and impact of maize breeding for food, feed, and fuel security. Without breeding programs continuously developing improved germplasm, no technology can develop improved cultivars. Quantitative Genetics in Maize Breeding presents principles and data that can be applied to maximize genetic improvement of germplasm and develop superior genotypes in different crops. The topics included should be of interest of graduate students and breeders conducting research not only on breeding and selection methods but also developing pure lines and hybrid cultivars in crop species. This volume is a unique and permanent contribution to breeders, geneticists, students, policy makers, and land-grant institutions still promoting quality research in applied plant breeding as opposed to promoting grant monies and indirect costs at any short-term cost. The book is dedicated to those who envision the development of the next generation of cultivars with less need of water and inputs, with better nutrition; and with higher percentages of exotic germplasm as well as those that pursue independent research goals before searching for funding. Scientists are encouraged to use all possible breeding methodologies available (e.g., transgenics, classical breeding, MAS, and all possible combinations could be used with specific sound long and short-term goals on mind) once germplasm is chosen making wise decisions with proven and scientifically sound technologies for assisting current breeding efforts depending on the particular trait under selection. Arnel R. Hallauer is C. F. Curtiss Distinguished Professor in Agriculture (Emeritus) at Iowa State University (ISU). Dr. Hallauer has led maize-breeding research for mid-season maturity at ISU since 1958. His work has had a worldwide impact on plant-breeding programs, industry, and students and was named a member of the National Academy of Sciences. Hallauer is a native of Kansas, USA. José B. Miranda Filho is full-professor in the Department of Genetics, Escola Superior de Agricultura Luiz de Queiroz - University of São Paulo located at Piracicaba, Brazil. His research interests have emphasized development of quantitative genetic theory and its application to maize breeding. Miranda Filho is native of Pirassununga, São Paulo, Brazil. M.J. Carena is professor of plant sciences at North Dakota State University (NDSU). Dr. Carena has led maize-breeding research for short-season maturity at NDSU since 1999. This program is currently one the of the few public U.S. programs left integrating pre-breeding with cultivar development and training in applied maize breeding. He teaches Quantitative Genetics and Crop Breeding Techniques at NDSU. Carena is a native of Buenos Aires, Argentina. http://www.ag.ndsu.nodak.edu/plantsci/faculty/Carena.htm

Molecular improvement of cereal crops

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Publisher : Springer Science & Business Media
ISBN 13 : 9401148023
Total Pages : 405 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Molecular improvement of cereal crops by : Indra K. Vasil

Download or read book Molecular improvement of cereal crops written by Indra K. Vasil and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the pre-historic era to modern times, cereal grains have been the most important source of human nutrition, and have helped sustain the increasing population and the development of human civilization. In order to meet the food needs of the 21st century, food production must be doubled by the year 2025, and nearly tripled by 2050. Such enormous increases in food productivity cannot be brought about by relying entirely on conventional breeding methods, especially on less land per capita, with poor quality and quantity of water, and under rapidly deteriorating environmental conditions. Complementing and supplementing the breeding of major food crops, such as the cereals, which together account for 66% of the world food supply, with molecular breeding and genetic manipulation may well provide a grace period of about 50 years in which to control population growth and achieve sustainable development. In this volume, leading world experts on cereal biotechnology describe the production and commercialization of the first generation of transgenic cereals designed to substantially reduce or prevent the enormous losses to cereal productivity caused by competition with weeds, and by various pests and pathogens, which is an important first step in that direction.

Breeding for drought and nitrogen stress tolerance in maize: From theory to practice

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Publisher : CIMMYT
ISBN 13 : 9706480463
Total Pages : 69 pages
Book Rating : 4.7/5 (64 download)

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Book Synopsis Breeding for drought and nitrogen stress tolerance in maize: From theory to practice by : M. Bänzinger

Download or read book Breeding for drought and nitrogen stress tolerance in maize: From theory to practice written by M. Bänzinger and published by CIMMYT. This book was released on 2000 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction - why breed for drought and low N tolerance?; Conceptual framework - breeding; Conventional approaches to improving the drought and low N tolerance of maize; Conventional approaches challenged; The challenge of breeding for drought and low N tolerance; Maize under drought and low N stress; Conceptual framework - physiology; Water and the maize plant; Nitrogen and the maize plant; Maize under drought and low N stress - consequences for breeding; Stress management; Drought; Low N stress; Statistical designs and layout of experiments; Increasing the number of replicates; Improved statistical designs; Field layout; Border effects from alleys; Secondary traits; Why use secondary traits?; How do we decide on the value of secondary traits in a drought or low N breeding program?; Secondary traits that help to identify drought tolerance; Secondary traits that help to identify low N tolerance: Selection indices - Combining information on secondary traits with grain yield; Combining information from various experiments; Breeding strategies; Choice of germplasm; Breeding schemes; Biotechnology: potential and constraints for improving drought and low N tolerance; The role of the farmer in selection; What is farmer participatory research and why is it important?; What is new about farmer participatory research?; Participatory methodologies.

Forages, Volume 2

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Publisher : John Wiley & Sons
ISBN 13 : 1119436613
Total Pages : 968 pages
Book Rating : 4.1/5 (194 download)

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Book Synopsis Forages, Volume 2 by : Kenneth J. Moore

Download or read book Forages, Volume 2 written by Kenneth J. Moore and published by John Wiley & Sons. This book was released on 2020-05-29 with total page 968 pages. Available in PDF, EPUB and Kindle. Book excerpt: Forages: The Science of Grassland Agriculture, 7th Edition, Volume II will extensively evaluate the current knowledge and information on forage agriculture. Chapters written by leading researchers and authorities in grassland agriculture are aggregated under section themes, each one representing a major topic within grassland science and agriculture. This 7th edition will include two new additional chapters covering all aspects of forage physiology in three separate chapters, instead of one in previous editions. Chapters will be updated throughout to include new information that has developed since the last edition. This new edition of the classic reference serves as a comprehensive supplement to An Introduction to Grassland Agriculture, Volume I.

The Impact of Plant Molecular Genetics

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Publisher : Springer Science & Business Media
ISBN 13 : 1461598559
Total Pages : 352 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis The Impact of Plant Molecular Genetics by : Bruno W.S. Sobral

Download or read book The Impact of Plant Molecular Genetics written by Bruno W.S. Sobral and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: The impact of molecular genetics on plant breeding and, consequently, agri culture, is potentially enonnous. Understanding and directing this potential im pact is crucial because of the urgent issues that we face concerning sustainable agriculture for a growing world population as well as conservation of the world's rapidly dwindling plant genetic resources. This book is largely devoted to the applications of genetic markers that have been developed by the application of molecular genetics to practical problems. These are known as DNA markers. They have gained a certain notoriety in foren sics, but can be used in a variety of practical situations. We are going through a period of accelerated breakthroughs in molecular ge netics. Therefore, the authors of each chapter were encouraged to speculate about both current bottlenecks and the future of their subfields of research. We can cer tainly apply molecular genetic tools and approaches to help resolve crucial ge netic resource problems that face humanity. However, little has been discussed with respect to when or how we should use such tools, nor to who specifically should use them; therefore, social and economic analyses are important in the planning stages of projects that are aimed at practical results.

Safety of Genetically Engineered Foods

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Publisher : National Academies Press
ISBN 13 : 0309166152
Total Pages : 254 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Safety of Genetically Engineered Foods by : National Research Council

Download or read book Safety of Genetically Engineered Foods written by National Research Council and published by National Academies Press. This book was released on 2004-07-08 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Assists policymakers in evaluating the appropriate scientific methods for detecting unintended changes in food and assessing the potential for adverse health effects from genetically modified products. In this book, the committee recommended that greater scrutiny should be given to foods containing new compounds or unusual amounts of naturally occurring substances, regardless of the method used to create them. The book offers a framework to guide federal agencies in selecting the route of safety assessment. It identifies and recommends several pre- and post-market approaches to guide the assessment of unintended compositional changes that could result from genetically modified foods and research avenues to fill the knowledge gaps.

Handbook of Maize

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Publisher : Springer Science & Business Media
ISBN 13 : 0387778632
Total Pages : 785 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Handbook of Maize by : Jeff L. Bennetzen

Download or read book Handbook of Maize written by Jeff L. Bennetzen and published by Springer Science & Business Media. This book was released on 2009-01-16 with total page 785 pages. Available in PDF, EPUB and Kindle. Book excerpt: Maize is one of the world’s highest value crops, with a multibillion dollar annual contribution to agriculture. The great adaptability and high yields available for maize as a food, feed and forage crop have led to its current production on over 140 million hectares worldwide, with acreage continuing to grow at the expense of other crops. In terms of tons of cereal grain produced worldwide, maize has been number one for many years. Moreover, maize is expanding its contribution to non-food uses, including as a major source of ethanol as a fuel additive or fuel alternative in the US. In addition, maize has been at the center of the transgenic plant controversy, serving as the first food crop with released transgenic varieties. By 2008, maize will have its genome sequence released, providing the sequence of the first average-size plant genome (the four plant genomes that are now sequenced come from unusually tiny genomes) and of the most complex genome sequenced from any organism. Among plant science researchers, maize has the second largest and most productive research community, trailing only the Arabidopsis community in scale and significance. At the applied research and commercial improvement levels, maize has no peers in agriculture, and consists of thousands of contributors worthwhile. A comprehensive book on the biology of maize has not been published. The "Handbook of Maize: the Genetics and Genomics" center on the past, present and future of maize as a model for plant science research and crop improvement. The books include brief, focused chapters from the foremost maize experts and feature a succinct collection of informative images representing the maize germplasm collection.

Analysis of the Maize (Zea Mays L.) Genome Using Molecular, Genetic and Computational Approaches

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Publisher :
ISBN 13 :
Total Pages : 236 pages
Book Rating : 4.:/5 (145 download)

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Book Synopsis Analysis of the Maize (Zea Mays L.) Genome Using Molecular, Genetic and Computational Approaches by : Yan Fu

Download or read book Analysis of the Maize (Zea Mays L.) Genome Using Molecular, Genetic and Computational Approaches written by Yan Fu and published by . This book was released on 2005 with total page 236 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approximately 80% of [difference]2,500 Mbp maize genome consists of highly repetitive sequences. Initial sequencing efforts have targeted the gene-rich regions of this genome. A new open reading frame (ORF) selecting vector (pORF-Rescue) was developed to efficiently distinguish between the coding and non-coding fractions of this complex genome. pORF-Rescue can enrich ORFs by [difference]15 fold and non-transposon coding sequences by [difference]3.6 fold. This vector can also be applied for rapid gene discovery from maize BAC DNA and to establish peptide/expression libraries. Methylation filtration (MF) and high-Cot (HC) sequencing strategies have also proven successful in maize gene-enrichment. To best utilize MF and HC genome survey sequences (GSSs), these GSS data were first examined by identifying types and frequencies of sequencing errors and then assembled into maize assembled genomic islands (MAGIs). Computational and biological quality assessments indicate that a very high percentage of the MAGIs accurately reflect the structure of the maize genome. In addition, we estimate that this assembly of the maize gene space has "tagged" >6,900 expressed genes that previously lacked evidence of transcription and that almost 350 of these expressed genes are "orphans"; i.e., they do not exhibit similarity to genes in other species. To construct a sequence-based maize genetic map using large-scale genie sequence data and intermated B73xMo17 recombinant inbred lines (IRILs) as mapping population will facilitate the map-based genome sequencing project, QTL and eQTL studies, and candidate gene cloning experiments. The observations that two segregation distortion regions overlap with maize flowering time QTLs suggest that the altered allele frequencies were a consequence of inadvertent selection during the development of the IBM IRILs. Detection of inter-chromosomal two-locus gamete disequilibrium suggests another means to discover potential functional relationships (e.g. epistasis) of different chromosome regions using well-characterized plant RILs.