Methods in Immunology and Immunochemistry

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Publisher : Elsevier
ISBN 13 : 0323148018
Total Pages : 502 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Methods in Immunology and Immunochemistry by : Curtis Williams

Download or read book Methods in Immunology and Immunochemistry written by Curtis Williams and published by Elsevier. This book was released on 2012-12-02 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Immunology and Immunochemistry, Volume I: Preparation of Antigens and Antibodies is aimed to bring together detailed procedures in the preparation of antigens and antibodies. The text also provides a presentation and discussion of these methods. The book covers topics in immunology such as antigens, its kinds, and the preparation and testing of lipids for immunological study. The production of antiserum; the preparation of immunogens; collecting and handling of serum; and immunization procedures are also explained. The book also discusses the purification of antibodies; methods of labeling antigens and antibodies; and the methods used in the studies of the structure of immunoglobulins. The text is recommended for immunologists who would like to know the different procedures and methods involved in immunology as well as the principles behind it. The book will also serve as a guide for medical staff who prepare products related to immunology.

Methods in Immunology and Immunochemistry

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Publisher :
ISBN 13 :
Total Pages : 479 pages
Book Rating : 4.:/5 (12 download)

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Book Synopsis Methods in Immunology and Immunochemistry by : Curtis A. Williams

Download or read book Methods in Immunology and Immunochemistry written by Curtis A. Williams and published by . This book was released on 1967 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Physical and Chemical Methods

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Publisher : Academic Press
ISBN 13 : 1483222993
Total Pages : 480 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Physical and Chemical Methods by : Curtis A. Williams

Download or read book Physical and Chemical Methods written by Curtis A. Williams and published by Academic Press. This book was released on 2014-05-12 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Immunology: Volume II, Physical and Chemical Methods is a collection of papers dealing with electrophoresis, analytical ultracentrifugation, dialysis, ultrafiltration, cellulose ion exchangers, and chromatographic separation of macromolecules on porous gels. Some papers explain the applications of radioisotopes, optical analysis, and chemical analysis of proteins, carbohydrates, lipids, and nucleic acid. One paper describes the theory of electro-migration. Factors such as electrical charge or frictional coefficients govern the rate of migration of charged particles in an electric field. The differences found in their velocities can be used to separate substances or analyze them. Mobility is a characteristic property of molecules and can also be influenced by the composition of the medium or solution. Dialysis separates solvents too large to diffuse through a barrier from smaller solutes; ultrafiltration (reverse osmosis) forces solvent and solutes up to a certain critical size through the barrier by a high pressure on one side. The book notes that the membrane never becomes plugged in dialysis because of some opposite movement of the solvent. Another paper points out that the significance of radioactive tracers in immunochemistry employed to identify and label macromolecules functioning as antigens and antibodies. The collection can prove valuable to bio-chemists, cellular biologists, micro-biologists, developmental biologists, and scientists involved in immunological research.

Reactions of Antibodies with Soluble Antigens

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Publisher : Academic Press
ISBN 13 : 1483220583
Total Pages : 536 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Reactions of Antibodies with Soluble Antigens by : Curtis A. Williams

Download or read book Reactions of Antibodies with Soluble Antigens written by Curtis A. Williams and published by Academic Press. This book was released on 2014-05-10 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Immunology and Immunochemistry, Volume III: Reactions of Antibodies with Soluble Antigens provides information pertinent to antigen–antibody and hapten–antibody reactions in vitro, in free solution and in gels. This book presents the development of research in immunology and immunochemistry. Organized into three chapters, this volume begins with an overview of protein–antiprotein reactions. This text then discusses the inhibitory activity of protein fragments, which suggested that antigenic combining sites of proteins were limited regions of the whole antigen molecule. Other chapters consider the measurement of inhibitory activity, which is still the principal assay to characterize antigenic sites of proteins. This book discusses as well the immunological techniques prior to the development of gel-diffusion methods. The final chapter deals with fluorescence labeling techniques that provide powerful approaches for exploring the thermodynamic and kinetic parameters of antigen–antibody interactions. This book is a valuable resource for mathematicians and immunologists.

Preparation of Antigens and Antibodies

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Publisher :
ISBN 13 :
Total Pages : 479 pages
Book Rating : 4.:/5 (62 download)

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Book Synopsis Preparation of Antigens and Antibodies by :

Download or read book Preparation of Antigens and Antibodies written by and published by . This book was released on 1971 with total page 479 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Methods in Immunology and Immunochemistry

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Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (144 download)

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Book Synopsis Methods in Immunology and Immunochemistry by : Curtis A. Williams

Download or read book Methods in Immunology and Immunochemistry written by Curtis A. Williams and published by . This book was released on 1977 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Immunochemistry Methods

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Publisher : LAP Lambert Academic Publishing
ISBN 13 : 9783659806513
Total Pages : 140 pages
Book Rating : 4.8/5 (65 download)

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Book Synopsis Immunochemistry Methods by : Tamara Krstic

Download or read book Immunochemistry Methods written by Tamara Krstic and published by LAP Lambert Academic Publishing. This book was released on 2015-11-18 with total page 140 pages. Available in PDF, EPUB and Kindle. Book excerpt: Book Immunochemistry methods consists of three main chapters (Introduction, Production of antigens and antibodies and Immunological tests). Each of three chapters has corresponding sub-continent. Introduction provides a brief, basic overview of the main components of the immune system. It gives a good basis for the display of techniques used in immunohistochemistry. Chapter Production of antigens and antibodies describes the sources of these components and methods for their preparation and purification, to very details. Chapter Immunological tests gives a comprehensive ilustration of reactions for identification and quantification of antigens. It also gives a brief overview of cellular immunological techniques and tests of complement. Its own experiance in the field, allows author to present these materials in comprehensive way. Theoretical definitions, descriptions of techniques and practical examples are of great importance for obtaining knowledge about the principles of immunohistochemistry. All this is illustrated by a large number of attractive and meaningful pictures, which allows the reader to visually experience all that is described.

Handbook of Immunochemistry

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Publisher : Springer Science & Business Media
ISBN 13 : 9401115524
Total Pages : 529 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Handbook of Immunochemistry by : Miroslav Ferencik

Download or read book Handbook of Immunochemistry written by Miroslav Ferencik and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 529 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book appeared in two previous Slovak editions for university students in Czechoslovakia. This edition presents a completely new version updated according to recent advances not only in immunochemistry and essential immunology but also in molecular biology, biochemistry and molecular genetics. The scope of the book is considerable since the goal was to cover the field of immunochemistry from the widest point of view including both the topic and methods of contemporary immunochemistry. Each chapter provides basic information on a specific subtopic, clearly and understandab ly, and presents principles of individual immunochemical methods. I am confident that the book will fill the gap between the books on essential immunology and highly specialised books on individual areas of immunoche mistry (e. g. on antibodies, antigens, numerous immunochemical techniques, etc. ). It may also prove useful for beginning investigators from different biological and medical fields as it supplies basic information needed for solving their scientific problems by immunochemical approaches. I do hope that readers will find the text stimulatory and pleasury to read. I wish to thank all colleagues and friends for supplying their own results, suggestions and for their encouraging comments. My thanks go also to the editors and publishers for their valuable contribution to the preparation of the book. 1 Introduction The term immunochemistry was coined by the Swedish chemist ARRHENIUS who used it for the first time in his lectures in 1907.

Methods in Immunology and Immunochemistry: Reactions of antibodies with soluble antigens

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Publisher :
ISBN 13 :
Total Pages : 550 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Methods in Immunology and Immunochemistry: Reactions of antibodies with soluble antigens by : Curtis Alvin Williams

Download or read book Methods in Immunology and Immunochemistry: Reactions of antibodies with soluble antigens written by Curtis Alvin Williams and published by . This book was released on 1967 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Methods in Immunology and Immunochemistry: Physical and chemical methods

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Publisher :
ISBN 13 :
Total Pages : 490 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Methods in Immunology and Immunochemistry: Physical and chemical methods by : Curtis Alvin Williams

Download or read book Methods in Immunology and Immunochemistry: Physical and chemical methods written by Curtis Alvin Williams and published by . This book was released on 1968 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic Methods in Antibody Production and Characterization

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Publisher : CRC Press
ISBN 13 : 142003653X
Total Pages : 286 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Basic Methods in Antibody Production and Characterization by : Gary C. Howard

Download or read book Basic Methods in Antibody Production and Characterization written by Gary C. Howard and published by CRC Press. This book was released on 2000-09-21 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written for researchers and professionals in the fields of biomedical research, immunology, biochemistry, molecular biology, pathology, and biotechnology, Basic Methods in Antibody Production and Characterization uses a cookbook approach to presenting the methods for the production, characterization, and use of antibodies. Antibodies described

Antigen-antibody Reactions in Vivo

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Publisher :
ISBN 13 : 9780127544052
Total Pages : 600 pages
Book Rating : 4.5/5 (44 download)

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Book Synopsis Antigen-antibody Reactions in Vivo by : Curtis Alvin Williams

Download or read book Antigen-antibody Reactions in Vivo written by Curtis Alvin Williams and published by . This book was released on 1976 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Band 5.

Basic Exercises in Immunochemistry

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Publisher : Springer Science & Business Media
ISBN 13 : 3662001489
Total Pages : 206 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Basic Exercises in Immunochemistry by : A. Nowotny

Download or read book Basic Exercises in Immunochemistry written by A. Nowotny and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 206 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book intends to be neither a complete survey of the field nor an exhaustive source of references. For these purposes, the use of the extensive compilation "Experimental Immunochemistry" by E. A. KABAT and M. M. MAYER (1962) or the excellent methodological textbook, "Methods in Immunology", by D. H. CAMPBELL, J. S. GARVEY, E. E. CREMER and D. H. SUSSDORF (1963), or the quite comprehensive series "Methods in Immunology and Immunochemistry" by C. A. WILLIAMS and M. W. CHASE (1967) are more suitable. The main purpose of this manual ist to provide students with a simple book which will introduce them to some frequently occurring problems in the three major sections of the immunochemistry of natural products. These are the isolation of the materials, the chemical analysis of the constituents and their structure, and, finally, the assays of the most important biological and immunological activities. In this manual the exercises are simplified and several shortcuts are taken in order to fit them into the framework of a teaching course. The introduction to each exercise gives a brief and elementary explanation of the reaction on which it is based. "Materials and Equipment" lists all tissues or cells, chemicals, glassware, and special equipment which must be available to carry out the exercise, although the very common laboratory tools are usually omitted from the list.

Immunological Methods

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Publisher : Academic Press
ISBN 13 : 148326999X
Total Pages : 492 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Immunological Methods by : Ivan Lefkovits

Download or read book Immunological Methods written by Ivan Lefkovits and published by Academic Press. This book was released on 2014-06-28 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunological Methods a compendium of basic research techniques being used in one of the largest immunology research institutes, the Basel Institute for Immunology, with particular emphasis given to new methodology. The procedures have been described by individuals judged to be highly expert in their specialties. In many instances the methods developed or adapted to unique uses by the contributors have not previously been described in detail. The book contains 34 chapters covering techniques for detection, isolation, and purification of antibodies (including dansylation, two-dimensional chromatography, isoelectric focusing, polyacrylamide gel electrophoresis, and isotachophoresis); measurement of equilibrium constants (equilibrium dialysis, filtration, and sedimentation); and isotope and fluorescent labeling and detection of cell-surface components. Techniques such as isotope laboratory maintenance; chemical modification of proteins, haptens, and solid supports, and haptenation of viable biological carriers; production of antisera against allotypes and histocompatibility antigens and production of antibody with clonai dominance; histocompatibility and MLR testing; and cell separation by haptenated gels and by velocity sedimentation of rosette-forming cells are also discussed. Other chapters cover detection of antibody-secreting and alloantigen-binding cells; immune responses in vitro and their analysis by limiting dilution; production of T-cell factors; hybridoma production by cell fusion; maintenance of cell lines and cloning in semisolid media; and the mathematical analysis of immunological data.

Handbook of Immunochemistry

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Publisher :
ISBN 13 : 9789401115537
Total Pages : 532 pages
Book Rating : 4.1/5 (155 download)

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Book Synopsis Handbook of Immunochemistry by : Miroslav Ferencik

Download or read book Handbook of Immunochemistry written by Miroslav Ferencik and published by . This book was released on 1998-07-22 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Antibody Techniques

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Publisher : Academic Press
ISBN 13 : 1483285138
Total Pages : 370 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis Antibody Techniques by : Vedpal S. Malik

Download or read book Antibody Techniques written by Vedpal S. Malik and published by Academic Press. This book was released on 2013-10-22 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: The applicability of immunotechniques to a wide variety of research problems in many areas of biology and chemistry has expanded dramatically over the last two decades ever since the introduction of monoclonal antibodies and sophisticated immunosorbent techniques. Exquisitely specific antibody molecules provide means of separation, quantitative and qualitative analysis, and localization useful to anyone doing biological or biochemical research. This practical guide to immunotechniques is especially designed to be easily understood by people with little practical experience using antibodies. It clearly presents detailed, easy-to-follow, step-by-step methods for the widely used techniques that exploit the unique properties of antibodies and will help researchers use antibodies to their maximum advantage. Detailed, easy-to-follow, step-by-step protocols Convenient, easy-to-use format Extensive practical information Essential background information Helpful hints

The Production of Antibodies

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Publisher :
ISBN 13 :
Total Pages : 162 pages
Book Rating : 4.E/5 ( download)

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Book Synopsis The Production of Antibodies by : Sir Frank Macfarlane Burnet

Download or read book The Production of Antibodies written by Sir Frank Macfarlane Burnet and published by . This book was released on 1949 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt: