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Mapping Of Resistance Genes In Barley Hordeum Vulgare L To Oat Stem Rust Pathogen Puccinia Graminis F Sp Avenae
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Book Synopsis Mapping of Resistance Genes in Barley (Hordeum Vulgare L.) to Oat Stem Rust Pathogen, Puccinia Graminis F. Sp. Avenae by : Moureen Nansamba
Download or read book Mapping of Resistance Genes in Barley (Hordeum Vulgare L.) to Oat Stem Rust Pathogen, Puccinia Graminis F. Sp. Avenae written by Moureen Nansamba and published by . This book was released on 2015 with total page 45 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Genetic Characterization of Virulence in a Pacific Northwest Stem Rust Population and Mapping of New Sources of Resistance in Barley by : Arjun Upadhaya
Download or read book Genetic Characterization of Virulence in a Pacific Northwest Stem Rust Population and Mapping of New Sources of Resistance in Barley written by Arjun Upadhaya and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem rust caused by the biotrophic fungal pathogen Puccinia graminis f. sp. tritici (Pgt) is an important disease of barley and wheat globally. In the last decade, the Pacific Northwest (PNW) region of the United States has experienced an increase in the incidence and severity of stem rust disease. This may be attributed to various factors, including increase in summer temperatures, lack of effective resistance, and early inoculum from sexual hosts. The PNW region's conducive environment and the presence of the sexual hosts, Mahonia and barberry, present an opportunity for the stem rust pathogen to complete its sexual cycle, leading to the emergence of genotypes with novel virulence gene combinations. A total of 100 Pgt isolates collected from barley in eastern Washington during the 2019 growing season were tested for virulence on the two major barley stem rust resistance genes/loci, Rpg1 and the rpg4/5-mediated resistance locus (RMRL) at the seedling stage. Of these isolates, 99% were virulent on cultivar Morex containing the Rpg1 gene, 62% were virulent on Golden Promise transgenic line (H228.2c) carrying a single copy of the Rpg1 gene from Morex, 16% were virulent on the near isogenic line, HQ-1 containing RMRL, and 10% were virulent on barley line Q21861 carrying both Rpg1 and RMRL. This was the first report of Pgt isolates containing virulence on both Rpg1 and RMRL when stacked together, representing the most virulent Pgt population reported on barley worldwide. To identify and map effective resistances against the local virulent isolates, a genome wide association study (GWAS) was conducted on 440 accessions from the World Barley Core Collection (WBCC), genotyped with the 9K Illumina barley iSelect chip and phenotyped with two PNW Pgt isolates at the seedling stage. A total of 10 resistance loci were identified including four novel loci on chromosomes 1H, 2H, 5H, and 6H. On the pathogen side, GWAS was performed using 113 diverse Pgt isolates, collected from the PNW and the Midwest region of the US, to identify virulence and avirulence loci corresponding to the barley stem rust resistance gene Rpg1 and the six wheat stem rust R-genes, Sr5, Sr21, Sr8a, Sr17, Sr9a, and Sr9d. The genotype data for 96 isolates were generated by whole genome shotgun sequencing and for 17 isolates by RNA sequencing. The phenotype data for the Pgt isolates were collected by seedling disease assays on two barley lines, H228.2c and Morex (Rpg1+) and the six wheat stem rust differential lines, Sr5, Sr21, Sr8a, Sr17, Sr9a, and Sr9d. A total of five effector gene candidates were identified at two loci corresponding to the Rpg1 gene. Similarly, 24 putative effector genes were identified at 15 loci corresponding to the six wheat R-genes. Further characterization of these genes will be done to enhance our understanding of host pathogen interactions in both the barley-Pgt and wheat-Pgt pathosystems.
Book Synopsis Investigation of Puccinia Graminis Resistance Genes in Barley by : Robert Saxon Brueggeman
Download or read book Investigation of Puccinia Graminis Resistance Genes in Barley written by Robert Saxon Brueggeman and published by . This book was released on 2009 with total page 88 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plant Pathogen Resistance Biotechnology by : David B. Collinge
Download or read book Plant Pathogen Resistance Biotechnology written by David B. Collinge and published by John Wiley & Sons. This book was released on 2016-06-13 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant pathogens and diseases are among the most significant challenges to survival that plants face. Disease outbreaks caused by microbial or viral pathogens can decimate crop yields and have severe effects on global food supply. Understanding the molecular mechanisms underlying plant immune response and applying this understanding to develop biotechnological tools to enhance plant defense against pathogens has great potential for moderating the impact of plant disease outbreaks. Plant Pathogen Resistance Biotechnology’s main focus is an in depth survey of the biological strategies being used to create transgenic disease resistant plants for sustainable plant resistance Plant Pathogen Resistance Biotechnology is divided into four sections. The first section covers biological mechanisms underpinning disease resistance in plants, while the second highlights case studies of important pathogen-crop groups and then considers why the application of important pathogen-crop groups, transgenic-based strategies designed to selectively target pathogens could benefit crop production. The third section provides information on the status of transgenic crops around the world, and finally the last part explores high-tech alternatives to genetic engineering for developing disease resistant traits in plants. Edited and authored by leaders in the field, Plant Pathogen Resistance Biotechnology will be an invaluable resource to those studying or researching plant biotechnology, plant pathology, plant biology, plant and crop genetics, in addition to crop science.
Book Synopsis Molecular Mapping of Stem Rust Resistance Genes in Wheat by :
Download or read book Molecular Mapping of Stem Rust Resistance Genes in Wheat written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Stem rust, caused by Puccinia graminis f. sp. tritici, has successfully prevented rust epidemics by Deployment of resistant cultivars in the past several decades. Unfortunately, race TTKS (termed Ug99) has defeated most stem rust resistance genes existing in commercial cultivars. Sr40, a stem rust resistance gene from Triticum timopheevii ssp. araraticum, was transferred to wheat and provides effective levels of seedling and adult plant resistance against Ug99. To characterize Sr40 in wheat, two mapping populations were developed from the crosses RL6088 / Lakin and RL6088 / 2174. RL6088 is an Ug99-resistant parent with Sr40. Since race TTKS is a quarantined pathogen, a US stem rust isolate RKQQ that is avirulent to Sr40 was used to evaluate the rust resistance in the F[subscript]2 and F[subscript]2:3 populations at the seedling stage. A total of 83 simple sequence repeats (SSR) primers on chromosome 2B were used to screen the parents for polymorphism. Each F[subscript]2 population was analyzed with the markers polymorphic between two parents. Marker Xwmc344 was the most closely linked to Sr40, at 0.7 cM proximal, in the linkage map constructed from the population RL6088 / Lakin, while Xwmc474 and Xgwm374 were also tightly linked. Xwmc474 was mapped 2.5 cM proximal to Sr40 in the RL6088 / 2174 population. Xwmc474 and Xwmc661 were flanking markers for Sr40 in both populations. Markers linked to Sr40 will be useful for marker-assisted integration of Sr40 into elite wheat breeding lines. In addition, a unknown stem rust resistance gene from another source, OK01307, a breeding line from Oklahoma State University shows partial resistance to Ug99, and was characterized using SSRs in this study. Two mapping populations were developed from cross OK01307 / Chinese Spring and OK01307 / LMPG-6. A total of 1300 SSR primers were screened for polymorphism between OK01307 and Chinese spring, and 1000 SSR primers were screened for polymorphism between OK01307 and LMPG-6. Polymorphic primers between parents and between bulks were used to screen the corresponding population. One Sr gene in OK01307 was mapped on chromosome 1BS of the both populations, which was closely linked to Sr24. Whether the gene is Sr24 per se or a new Sr gene that closely linked to Sr24 needs further investigation.
Book Synopsis Nonhost Resistance to Rusts (Puccina Sp.) in Barley (Hordeum Vulgare L.) by : D. Bomhoff
Download or read book Nonhost Resistance to Rusts (Puccina Sp.) in Barley (Hordeum Vulgare L.) written by D. Bomhoff and published by . This book was released on 2014 with total page 95 pages. Available in PDF, EPUB and Kindle. Book excerpt: The identification of genes conferring nonhost resistance (NHR) is a crucial step in its understanding and usage in breeding. The Barley(Hordeum vulgare L.)-Puccinia (rust fungi) model system uses marginal hosts of barley to Puccinia rusts to study NHR, making the identification of NHR genes possible. This study entails the fine mapping work of two NHR genes; one located on the introgression of the NIL SusQ11, near Rphq11, and the other located on L94.
Book Synopsis Mapping and Introgression of Disease Resistance Genes in Barley (Hordeum Vulgare L.) by : Theeryut Toojinda
Download or read book Mapping and Introgression of Disease Resistance Genes in Barley (Hordeum Vulgare L.) written by Theeryut Toojinda and published by . This book was released on 1998 with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular tools, coupled with unique germplasm stocks and rigorous phenotyping, are useful for developing a better understanding of qualitative and quantitative disease resistance genes in plants. The identification of molecular markers linked to all types of resistance genes provides opportunities for implementing a range of resistance breeding strategies, ranging from gene pyramiding to gene deployment. This thesis consists of two chapters. The first describes a disease resistance gene mapping effort and the second describes a disease resistance gene introgression effort. The number, location, and effects of genes determining resistance to stripe rust, leaf rust and Barley Yellow Dwarf Virus were determined using a population of doubled haploid (DH) lines from the cross of Shyri x Galena. Resistance to leaf rust was qualitatively inherited, and the locus was mapped to the long arm of chromosome 1. Resistance to stripe rust and BYDV was quantitatively inherited. Multiple QTLs were detected for each type of resistance. The principal stripe rust resistance QTL was on the short arm of chromosome 5 and the principal BYDV resistance QTL was on the long arm of chromosome 1, linked in repulsion phase with the leaf rust resistance gene. Additional QTLs and QTL x QTL interactions were detected. The majority of the qualitative and quantitative resistance loci detected in the Shyri x Galena population coincided with Resistance Gene Analog Polymorphisms (RGAPs) mapped in the same population. These RGAPs were based on degenerate primers derived from cloned resistance gene sequence motifs. These associations should be useful for efficient resistance gene mapping and provide an approach for ultimately isolating and describing quantitative and qualitative resistance genes. The second chapter describes a molecular marker assisted selection (MMAS) effort to introgress stripe rust resistance QTLs on chromosomes 4 and 7 into susceptible germplasm. DH lines were derived form a MMAS backcross-one (BC-1) population, extensively phenotyped for stripe rust resistance, and genotyped for the introgressed QTLs and background genome. The resistance QTLs that were introgressed were significant determinants of resistance in the new genetic background. Additional resistance QTLs were also detected. Together, these chapters describe an integrated approach to disease resistance gene characterization and utilization.
Book Synopsis Identification of the Gene Underlying the Rphq2 Resistance of Barley (Hordeum Vulgare) to Barley Leaf Rust (Puccinia Hordei) by : Sudeep Subedi
Download or read book Identification of the Gene Underlying the Rphq2 Resistance of Barley (Hordeum Vulgare) to Barley Leaf Rust (Puccinia Hordei) written by Sudeep Subedi and published by . This book was released on 2017 with total page 44 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Understanding and Characterizing the Genes Associated with RPG1-mediated Resistance Pathway Against Stem Rust by : Upinder Singh Gill
Download or read book Understanding and Characterizing the Genes Associated with RPG1-mediated Resistance Pathway Against Stem Rust written by Upinder Singh Gill and published by . This book was released on 2012 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Barley stem rust resistance gene, Rpg1 (Reaction to Puccinia graminis 1), confers durable resistance to barley cultivars against stem rust caused by many races of Puccinia graminis f. sp. tritici. The current study was focused on understanding and characterizing the genes associated with Rpg1 in barley. HvRIN4 (a barley homolog of RPM1 interacting protein in Arabidopsis), which interacts with RPG1 in an yeast two-hybrid system, was isolated, characterized and studied for its role in barley. Using gamma-ray mutagenesis, six mutants affecting the Rpg1-mediated resistance against stem rust were identified. All mutants retained a functional Rpg1 gene and were non-allelic, suggesting that the mutated genes were required for downstream or upstream signaling. Low and high-resolution genetic mapping of the mutant gamma 08--118 gene identified a 0.6 cM region of chromosome 6H of barley for its location. In another study the applicability of wheat Conserved Orthologous Set (COS) markers on diverse cereal germplasm and their efficacy was investigated. Overall, this research will help in understanding the mechanism of Rpg1-mediated durable resistance against stem rust in barley.
Book Synopsis Molecular Mapping of Barley Genes for Resistance to Stripe Rust by : Guiping Yan
Download or read book Molecular Mapping of Barley Genes for Resistance to Stripe Rust written by Guiping Yan and published by . This book was released on 2006 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt: Stripe rust, caused by Puccinia striiformis f. sp. hordei, is one of the most important diseases of barley ( Hordeum vulgare). Growing resistant cultivars is the best approach for controlling stripe rust. Grannenlose Zweizeilige (GZ) and BBA 2890 each have a recessive gene, temporarily designated as rpsGZ in GZ and rps1.a in BBA 2890, conferring seedling resistance against all races of P. striiformis f. sp. hordei identified thus far in the US. Bancroft was one of the first barley cultivars developed in the US with resistance to stripe rust, and has been identified as having durable high-temperature, adult-plant (HTAP) resistance.
Book Synopsis Studies of the Pathogenic Potentialities of Puccinia Graminis Avenae (oat Stem Rust) and Genetics of Resistance in Oats by : Instituto Colombiano Agropecuario
Download or read book Studies of the Pathogenic Potentialities of Puccinia Graminis Avenae (oat Stem Rust) and Genetics of Resistance in Oats written by Instituto Colombiano Agropecuario and published by . This book was released on 1967 with total page 92 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification and Mapping of Resistance Genes Against Soil-borne Viruses in Barley (Hordeum Vulgare L.) and Wheat (Triticum Aestivum L.) by : Katrin Humbroich
Download or read book Identification and Mapping of Resistance Genes Against Soil-borne Viruses in Barley (Hordeum Vulgare L.) and Wheat (Triticum Aestivum L.) written by Katrin Humbroich and published by . This book was released on 2007 with total page 109 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Plant Relationships by : Barry Scott
Download or read book Plant Relationships written by Barry Scott and published by Springer Nature. This book was released on 2022-12-08 with total page 471 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully revised 3rd edition provides a comprehensive overview of the biology of fungi associated with plants. Since the publication of the 2nd Edition in 2009, tremendous new knowledge has been gained in the field of fungal-plant interactions, which is reflected in the contributions of this book. World-leading scientists in the field provide authoritative insights into fungal-plant interactions covering the following main topics: Mutualistic and pathogenic fungal-plant interactions in natural and agricultural ecosystems Sensing and signalling in fungus-plant interactions Regulation of fungal gene expression and development Fungal genomes and evolution Global pandemics caused by fungal pathogens and their implications for food security This volume will be of great interest to both specialists and generalists. It is an indispensable resource for researchers, lecturers and students in microbiology, mycology, and plant sciences, as well as agriculture and biotechnology.
Download or read book Crop Science written by and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis High Resolution Mapping of RphMBR1012 Conferring Resistance to Puccinia Hordei in Barley (Hordeum Vulgare L.) by : Leila Fazlikhani
Download or read book High Resolution Mapping of RphMBR1012 Conferring Resistance to Puccinia Hordei in Barley (Hordeum Vulgare L.) written by Leila Fazlikhani and published by . This book was released on 2020* with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Barley, leaf rust resistance gene RphMBR1012, positional isolation.
Book Synopsis The Genetics of Host by : Adrian Lester Cabral
Download or read book The Genetics of Host written by Adrian Lester Cabral and published by . This book was released on 2009 with total page 430 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Cereal Rusts: Origins, specificity, structure, and physiology by : William Rodgers Bushnell
Download or read book The Cereal Rusts: Origins, specificity, structure, and physiology written by William Rodgers Bushnell and published by . This book was released on 1984 with total page 576 pages. Available in PDF, EPUB and Kindle. Book excerpt: Origins, specificity, structure, and physiology; Evolution at the center of origin; Taxonomy of the cereal rust fungi; Specificity; The formae speciales; Race specificity and methods of study; Genetics of the pathogen: host association; Histology and molecular biology of host: parasite; Virulence frequency dynamics of cereal rust fungi; The rust fungus; Controlled infection by Puccinia graminis f.sp. tritici under artificial conditions; Developmental ultrastructure of hyphae and spores; Development and physical of teliospores; Obligate parasitism and axenic culture; The host parsite interface; The rusted host; Effects of rust on plant development in relation to the translocation of inorganic and organic solutes.
