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Manipulation Of Micro Scale Particles In An Optical Trap Using Interferometry
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Author :National Aeronautics and Space Administration (NASA) Publisher :Createspace Independent Publishing Platform ISBN 13 :9781721529995 Total Pages :24 pages Book Rating :4.5/5 (299 download)
Book Synopsis Manipulation of Micro Scale Particles in an Optical Trap Using Interferometry by : National Aeronautics and Space Administration (NASA)
Download or read book Manipulation of Micro Scale Particles in an Optical Trap Using Interferometry written by National Aeronautics and Space Administration (NASA) and published by Createspace Independent Publishing Platform. This book was released on 2018-06-20 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt: This research shows that micro particles can be manipulated via interferometric patterns superimposed on an optical tweezers beam. Interferometry allows the manipulation of intensity distributions, and thus, force distributions on a trapped particle. To demonstrate the feasibility of such manipulation, 458 nm light, from an argon-ion laser, was injected into a Mach Zender interferometer. One mirror in the interferometer was oscillated with a piezoelectric phase modulator. The light from the interferometer was then injected into a microscope to trap a 9.75 micron polystyrene sphere. By varying the phase modulation, the sphere was made to oscillate in a controlled fashion. Seibel, Robin Glenn Research Center NASA/CR-2002-211975, NAS 1.26:211975, E-13644
Book Synopsis Manipulation of Micro Scale Particles in an Optical Trap Using Interferometry by :
Download or read book Manipulation of Micro Scale Particles in an Optical Trap Using Interferometry written by and published by . This book was released on 2002 with total page 12 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :National Aeronautics and Space Administration (NASA) Publisher :Createspace Independent Publishing Platform ISBN 13 :9781721605248 Total Pages :24 pages Book Rating :4.6/5 (52 download)
Book Synopsis Manipulation of Micro Scale Particles in Optical Traps Using Programmable Spatial Light Modulation by : National Aeronautics and Space Administration (NASA)
Download or read book Manipulation of Micro Scale Particles in Optical Traps Using Programmable Spatial Light Modulation written by National Aeronautics and Space Administration (NASA) and published by Createspace Independent Publishing Platform. This book was released on 2018-06-20 with total page 24 pages. Available in PDF, EPUB and Kindle. Book excerpt: 1064 nm light, from an Nd:YAG laser, was polarized and incident upon a programmable parallel aligned liquid crystal spatial light modulator (PAL-SLM), where it was phase modulated according to the program controlling the PAL-SLM. Light reflected from the PAL-SLM was injected into a microscope and focused. At the focus, multiple optical traps were formed in which 9.975 m spheres were captured. The traps and the spheres were moved by changing the program of the PAL-SLM. The motion of ordered groups of micro particles was clearly demonstrated. Seibel, Robin E. and Decker, Arthur J. (Technical Monitor) Glenn Research Center NASA/CR-2003-212726, E-14250
Book Synopsis Optical Trapping and Manipulation of Neutral Particles Using Lasers by : Arthur Ashkin
Download or read book Optical Trapping and Manipulation of Neutral Particles Using Lasers written by Arthur Ashkin and published by World Scientific Publishing Company Incorporated. This book was released on 2006 with total page 915 pages. Available in PDF, EPUB and Kindle. Book excerpt: This important volume contains selected papers and extensive commentaries on laser trapping and manipulation of neutral particles using radiation pressure forces. Such techniques apply to a variety of small particles, such as atoms, molecules, macroscopic dielectric particles, living cells, and organelles within cells. These optical methods have had a revolutionary impact on the fields of atomic and molecular physics, biophysics, and many aspects of nanotechnology.In atomic physics, the trapping and cooling of atoms down to nanokelvins and even picokelvin temperatures are possible. These are the lowest temperatures in the universe. This made possible the first demonstration of Bose-Einstein condensation of atomic and molecular vapors. Some of the applications are high precision atomic clocks, gyroscopes, the measurement of gravity, cryptology, atomic computers, cavity quantum electrodynamics and coherent atom lasers.A major application in biophysics is the study of the mechanical properties of the many types of motor molecules, mechanoenzymes, and other macromolecules responsible for the motion of organelles within cells and the locomotion of entire cells. Unique in vitro and in vivo assays study the driving forces, stepping motion, kinetics, and efficiency of these motors as they move along the cell's cytoskeleton. Positional and temporal resolutions have been achieved, making possible the study of RNA and DNA polymerases, as they undergo their various copying, backtracking, and error correcting functions on a single base pair basis.Many applications in nanotechnology involve particle and cell sorting, particle rotation, microfabrication of simple machines, microfluidics, and other micrometer devices. The number of applications continues to grow at a rapid rate.The author is the discoverer of optical trapping and optical tweezers. With his colleagues, he first demonstrated optical levitation, the trapping of atoms, and tweezer trapping and manipulation of living cells and biological particles.This is the only review volume covering the many fields of optical trapping and manipulation. The intention is to provide a selective guide to the literature and to teach how optical traps really work.
Book Synopsis Optical Tweezers by : Philip H. Jones
Download or read book Optical Tweezers written by Philip H. Jones and published by Cambridge University Press. This book was released on 2015-12-03 with total page 565 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive guide to the theory, practice and applications of optical tweezers, combining state-of-the-art research with a strong pedagogic approach.
Book Synopsis Optical Trapping And Manipulation Of Neutral Particles Using Lasers: A Reprint Volume With Commentaries by : Arthur Ashkin
Download or read book Optical Trapping And Manipulation Of Neutral Particles Using Lasers: A Reprint Volume With Commentaries written by Arthur Ashkin and published by World Scientific. This book was released on 2006-12-29 with total page 941 pages. Available in PDF, EPUB and Kindle. Book excerpt: This important volume contains selected papers and extensive commentaries on laser trapping and manipulation of neutral particles using radiation pressure forces. Such techniques apply to a variety of small particles, such as atoms, molecules, macroscopic dielectric particles, living cells, and organelles within cells. These optical methods have had a revolutionary impact on the fields of atomic and molecular physics, biophysics, and many aspects of nanotechnology.In atomic physics, the trapping and cooling of atoms down to nanokelvins and even picokelvin temperatures are possible. These are the lowest temperatures in the universe. This made possible the first demonstration of Bose-Einstein condensation of atomic and molecular vapors. Some of the applications are high precision atomic clocks, gyroscopes, the measurement of gravity, cryptology, atomic computers, cavity quantum electrodynamics and coherent atom lasers.A major application in biophysics is the study of the mechanical properties of the many types of motor molecules, mechanoenzymes, and other macromolecules responsible for the motion of organelles within cells and the locomotion of entire cells. Unique in vitro and in vivo assays study the driving forces, stepping motion, kinetics, and efficiency of these motors as they move along the cell's cytoskeleton. Positional and temporal resolutions have been achieved, making possible the study of RNA and DNA polymerases, as they undergo their various copying, backtracking, and error correcting functions on a single base pair basis.Many applications in nanotechnology involve particle and cell sorting, particle rotation, microfabrication of simple machines, microfluidics, and other micrometer devices. The number of applications continues to grow at a rapid rate.The author is the discoverer of optical trapping and optical tweezers. With his colleagues, he first demonstrated optical levitation, the trapping of atoms, and tweezer trapping and manipulation of living cells and biological particles.This is the only review volume covering the many fields of optical trapping and manipulation. The intention is to provide a selective guide to the literature and to teach how optical traps really work.
Book Synopsis Single Particle Studies Using Optical Trapping and Manipulation by : Zhiyong Gong
Download or read book Single Particle Studies Using Optical Trapping and Manipulation written by Zhiyong Gong and published by . This book was released on 2019 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Particles of nanometer- and micrometer-size, including diverse types of aerosols, dusts, and material powders, pose significant influence on environment, climate, human health, and material properties. Our understanding towards these particles and materials is mainly established from bulk samples and from particles deposited on substrates or dispersed in liquid solutions. However, fundamental properties and interactions of single particles cannot be fully resolved by characterizing bulk materials or deposited particles. Pristine physicochemical properties and rudimentary gas-particle interactions can only be investigated from single particles in their native states. The goal of this dissertation is to study single particles in gaseous environment with minimum external interferences. Diverse optical trapping (OT) methods for trapping and manipulating single airborne particles were explored and summarized. These configurations are categorized into radiation-pressure traps, photophoretic traps, and universal optical traps (UOT), according to particle’s absorbance. The UOT configurations are able to trap and manipulate a wide variety of single particles, including transparent and absorbing, spherical and irregular, inorganic and biological particles. Four key aspects of these OTs, simplicity, robustness, flexibility, and efficiency, are brought up to evaluate OT capabilities, providing a guidance to select the optimal optical configuration for specific applications. With single particles stably trapped, optical manipulations and characterizations can therefore be interrogated. Single-particle manipulations in air from one-dimension to three-dimension are reviewed; pulling and pushing single optically trapped particles along a Gaussian laser beam were explored and explained. Cavity ringdown spectroscopy is integrated with OTs to measure the light extinctions of trapped particles. Microscopic imaging and Raman systems are combined with OTs to examine the trapped particles. A number of application examples of this optical trapping-Raman spectroscopy system are demonstrated, including online characterizing carbon particles, effectively bleaching fluorescence in dye-doped polymer microspheres, acquiring clean Raman spectra of single biological particles, and monitoring chemical reactions in single biological particles. Optical trapping, associated with advanced laser spectroscopic techniques, offer a great flexibility for trapping, manipulating, characterizing, and monitoring single particles in air.
Book Synopsis Calibration of an Optical Trap by : Debalina Chakraborty
Download or read book Calibration of an Optical Trap written by Debalina Chakraborty and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: A tightly focused laser beam can trap and manipulate microscopic objects by exerting force on them. Optical trapping or laser trapping or laser tweezers is a term widely used in describing the trapping and manipulation of these objects by optical gradient forces. Such devices have many uses in biophysics. This thesis describes the reconstruction of a previously existing optical trapping system that had fallen into disrepair and the work done to calibrate that optical trapping system. The pre-existing system was refurbished and aligned so that optical traps could again be generated. The resulting traps were then calibrated in preparation for further improvements to the trapping strength. Micrometer-sized dielectric spheres were trapped by the tweezers. Trap stiffness was measured using a mean square displacement method from data obtained with two particle tracking programs operating on captured microscope image stacks. The trap stiffnesses thus obtained were checked by applying known hydrodynamic forces to the particles and measuring the displacement. The results show that both the tracking programs (Rytrack and Sphere tracker)generated trap stiffness values closer to the results from hydrodynamic displacement. Trap stiffness was also found to increase with increasing laser power, as expected. The stiffness vs power can be fit by a power law with an exponent of 0.73 for Rytrack and 0.66 for Sphere tracker. The trap stiffness was also weakly dependent on height above the sample plane. Surprisingly, it was found that trap strength differed by some significant factors (sometimes as much as a factor of 2) along perpendicular axes. The source of this asymmetrical trap strength has yet to be determined. In addition, the apparent uncertainty in the trap strength data is much larger than the expected uncertainty from the mean square displacement. Mean square displacement seemed to vary with time. This left a source of systematic error seen in the values of trap stiffness. The source of this additional uncertainty is not clear but may lie in time variations in laser power. This will also be a subject of further work.
Book Synopsis Optical Trapping and Manipulation by : Philip Jones
Download or read book Optical Trapping and Manipulation written by Philip Jones and published by MDPI. This book was released on 2020-11-25 with total page 128 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are pleased to present “Optical Trapping and Manipulation: From Fundamentals to Applications”, a Special Issue of Micromachines dedicated to the latest research in optical trapping. In recognition of the broad impact of optical manipulation techniques across disciplines, this Special Issue collected contributions related to all aspects of optical trapping and manipulation. Both theoretical and experimental studies were welcome, and applications of optical manipulation methods in fields including (but not limited to) single molecule biophysics, cell biology, nanotechnology, atmospheric chemistry, and fundamental optics were particularly welcome in order to showcase the breadth of the current research. The Special Issue accepted diverse forms of contributions, including research papers, short communications, methods, and review articles representing the state-of-the-art in optical trapping.
Book Synopsis Manipulation of Particles by Optical Trapping by : Boya Zhang
Download or read book Manipulation of Particles by Optical Trapping written by Boya Zhang and published by . This book was released on 2017 with total page 146 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Counter-Propagating Optical Trapping System for Size and Refractive Index Measurement of Microparticles by :
Download or read book Counter-Propagating Optical Trapping System for Size and Refractive Index Measurement of Microparticles written by and published by . This book was released on 2005 with total page 9 pages. Available in PDF, EPUB and Kindle. Book excerpt: We propose and demonstrate a novel approach to measure the size and refractive index of microparticles based on two beam optical trapping, where forward scattered light is detected to give information about the particle. The counter-propagating optical trap measurement (COTM) system exploits the capability of optical traps to measure pico-Newton forces for microparticles refractive index and size characterization. Different from the current best technique for microparticles refractive index measurement, refractometry, a bulk technique requiring changing the fluid composition of the sample, our optical trap technique works with any transparent fluid and enables single particle analysis without the use of biological markers. A ray-optics model is used to explore the physical operation of the COTM system, predict system performance and aid system design. Experiments demonstrate the accuracy of refractive index measurement of triangel n = 0.013 and size measurement of 3% of diameter with 2% standard deviation. Present performance is instrumentation limited, and a potential improvement by more than two orders of magnitude can be expected in the future. With further development in parallelism and miniaturization, the system offers advantages for cell manipulation and bioanalysis compatible with lab-on-a-chip systems.
Book Synopsis Optical Levitation of Micro-Scale Particles in Air by : Susan Y. Wrbanek
Download or read book Optical Levitation of Micro-Scale Particles in Air written by Susan Y. Wrbanek and published by BiblioGov. This book was released on 2013-07 with total page 22 pages. Available in PDF, EPUB and Kindle. Book excerpt: Success has been achieved using a radiation pressure gradient to levitate microscale particles in air for as long as four hours. This work is performed as a precursor to the development of a vacuum based optical tweezers interrogation tool for nanotechnology research. It was decided to first proceed with solving the problem of achieving optical levitation of a micro-scale particle in air before trying the same in a vacuum environment. This successful optical levitation in air confirms the work of Ashkin and Dziedzic. Levitation of 10 and 13.8 microns diameter polystyrene spheres was achieved, as well as the levitation of 10 and 100 microns diameter glass spheres. Particles were raised and lowered. A modicum of success was achieved translating particles horizontally. Trapping of multiple particles in one laser beam has been photographed. Also, it has been observed that particles, that may be conglomerates or irregular in shape, can also be trapped by a focused laser beam. Levitated glass beads were photographed using laser light scattered from the beads. The fact that there is evidence of optical traps in air containing irregular and conglomerate particles provides hope that future tool particles need not be perfect spheres.
Book Synopsis Monthly Catalog of United States Government Publications by :
Download or read book Monthly Catalog of United States Government Publications written by and published by . This book was released on 2003 with total page 620 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Back Focal Plane Interferometry for Holographic Optical Traps by : Jade Martínez Llinàs
Download or read book Back Focal Plane Interferometry for Holographic Optical Traps written by Jade Martínez Llinàs and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: English: Since its invention in 1986, optical trapping as a tool to manipulate microparticles non-invasively has found many applications in physics and biology. Force measurement on biomaterials is a potential application of optical tweezers which requires precise position detection. Back focal plane interferometry has become a commonly precise method for position detection, but it is limited to single trap calibration. However, the study of complex biological systems requires simultaneous multiple trap manipulation. Time-sharing a trap at kHz by acousto-optic-deflectors produces multiple independent tweezers, but limited to two dimensions. In this work, we aim to combine the versatility of digital holography for simultaneous multiple manipulation with the precision of back focal plane interferometry for force measurement. A holographic optical system set-up has been built and used to generate two traps with orthogonal polarizations: a fixed trap useful for measuring forces, and a holographic tweezer which allows dynamic manipulation. This method represents a step towards simultaneous manipulation with many holographic traps and precise position detection with only one trap.
Book Synopsis Microrheology with Optical Tweezers by : Manlio Tassieri
Download or read book Microrheology with Optical Tweezers written by Manlio Tassieri and published by CRC Press. This book was released on 2016-10-14 with total page 356 pages. Available in PDF, EPUB and Kindle. Book excerpt: Thanks to the pioneering works of Ashkin and coworkers, optical tweezers (OTs) have become an invaluable tool for myriad studies throughout the natural sciences. Their success relies on the fact that they can be considered as exceptionally sensitive transducers that are able to resolve pN forces and nm displacements, with high temporal resolution, down to μs. Hence their application to study a wide range of biological phenomena such as measuring the compliance of bacterial tails, the forces exerted by a single motor protein, and the mechanical properties of human red blood cells and of individual biological molecules. The number of articles related to them totals to a whopping 58,000 (source Google Scholar)! Microrheology is a branch of rheology, but it works at micrometer length scales and with microliter sample volumes. Therefore, microrheology techniques have been revealed to be very useful tools for all those rheological/mechanical studies where rare or precious materials are employed, such as in biological and biomedical studies. The aim of this book is to provide a pedagogical introduction to the physics principles governing both the optical tweezers and their application in the field of microrheology of complex materials. This is achieved by following a linear path that starts from a narrative introduction of the "nature of light," followed by a rigorous description of the fundamental equations governing the propagation of light through matter. Moreover, some of the many possible instrumental configurations are presented, especially those that better adapt to perform microrheology measurements. In order to better appreciate the microrheological methods with optical tweezers explored in this book, informative introductions to the basic concepts of linear rheology, statistical mechanics, and the most popular microrheology techniques are also given. Furthermore, an enlightening prologue to the general applications of optical tweezers different from rheological purposes is provided at the end of the book.
Book Synopsis Micro and Nano Techniques for the Handling of Biological Samples by : Jaime Castillo-León
Download or read book Micro and Nano Techniques for the Handling of Biological Samples written by Jaime Castillo-León and published by CRC Press. This book was released on 2011-08-25 with total page 258 pages. Available in PDF, EPUB and Kindle. Book excerpt: Several micro- and nanomanipulation techniques have emerged in recent decades thanks to advances in micro- and nanofabrication. For instance, the atomic force microscope (AFM) uses a nano-sized tip to image, push, pull, cut, and indent biological material in air, liquid, or vacuum. Using micro- and nanofabrication techniques, scientists can make manipulation tools, such as microgrippers and nanotweezers, on the same length scale as the biological samples. Micro and Nano Techniques for the Handling of Biological Samples reviews the different techniques available to manipulate and integrate biological materials in a controlled manner, either by sliding them along a surface (2-D manipulation), or by gripping and moving them to a new position (3-D manipulation). The advantages and drawbacks are mentioned together with examples that reflect the state-of-the-art in manipulation techniques for biological samples. Thanks to the advances in micro- and nanomanipulation techniques, the integration of biomaterials with physical transducers has been possible, giving rise to new and highly sensitive biosensing devices. Although great progress has been made, challenges are still present. To understand the complex interactions between and inside biological samples, scientists will always be working on improving technologies to manipulate, transport, sort, and integrate samples in different environments. Balanced between simplicity for the beginner and hardcore theory for the more advanced readers, this book is the ideal launching point for sharpening the scientific tools required to address these challenges.
Book Synopsis Optical Tweezers by : Miles J. Padgett
Download or read book Optical Tweezers written by Miles J. Padgett and published by CRC Press. This book was released on 2010-06-02 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technical development of optical tweezers, along with their application in the biological and physical sciences, has progressed significantly since the demonstration of an optical trap for micron-sized particles based on a single, tightly focused laser beam was first reported more than twenty years ago. Bringing together many landmark papers on
