Lipase And Phospholipase Protocols

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Lipase and Phospholipase Protocols

Author : Mark Doolittle
Publisher : Springer Science & Business Media
ISBN 13 : 1592595812
Total Pages : 362 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Lipase and Phospholipase Protocols by : Mark Doolittle

Download or read book Lipase and Phospholipase Protocols written by Mark Doolittle and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: The lipases and phospholipases represent a diverse group of enzymes that are expressed in animals, plants, fungi, and bacteria. Their ubiquitous distribution among all species is a testament to the essential roles played by these enzymes in lipid storage, mobilization and metabolism, membrane homeostasis and remodeling, endocrine and immune functions, and signal tra- duction. In humans, lipases and phospholipases are also thought to contribute to complex diseases, such as atherosclerosis, obesity, arthritis, and cancer, as well as to single gene defects, such as Wolman's disease and Type I hyperlipoproteinemia. Enzymatically, the lipases are unique, since they hydrolyze substrates that are either insoluble, or only partly soluble, in aq- ous solvents; thus, enzyme catalysis takes place at a lipid-water interface. The interface comprises at least two, and often more, discrete bulk and s- face phases, in which the enzyme, substrate, and products oflipolysis disperse among these phases based on their physical properties. Furthermore, the d- tribution of these components changes continuously as lipolysis proceeds. Thus, the lipases and phospholipases are fundamentally different from any other enzyme because of the physical complexity of the environment in which catalysis occurs.

Lipases and Phospholipases

Author : Georgina Sandoval
Publisher : Humana
ISBN 13 : 9781493993611
Total Pages : 437 pages
Book Rating : 4.9/5 (936 download)

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Book Synopsis Lipases and Phospholipases by : Georgina Sandoval

Download or read book Lipases and Phospholipases written by Georgina Sandoval and published by Humana. This book was released on 2019-12-10 with total page 437 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides an essential update on fundamental issues, current and new applications, as well as practical protocols to explore the extensive applications of lipases and the potential application of phospholipases. After an overview, the book delves into activity screening and expression, optimization of the biocatalyst production and performances, and applications of lipases, phospholipases, and esterases. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Lipases and Phospholipases: Methods and Protocols, Second Editionserves as an updated reference book for the large scientific community, both seasoned and novice, working with lipases, phospholipases, and related enzymes.

Lipases and Phospholipases in Drug Development

Author : Günter Müller
Publisher : John Wiley & Sons
ISBN 13 : 3527605703
Total Pages : 354 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Lipases and Phospholipases in Drug Development by : Günter Müller

Download or read book Lipases and Phospholipases in Drug Development written by Günter Müller and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases and Phospholipases are key control elements in mammalian metabolism. They share many common features that set them apart from other metabolic enzyme classes, most importantly their association with biological membranes. Their potential as drug targets for the treatment of metabolic diseases is widely recognized, and the first lipase inhibitor drugs have been successfully introduced. Providing drug developers with a firm foundation for lipase-centered drug design, the editors of this volume have assembled experts from different scientific disciplines to create a comprehensive handbook for all pharmaceutical chemists, biochemists and physiologists working with lipases. The authors examine fundamental aspects of lipase function in vitro and in vivo, explaining how this knowledge may be used to develop lipase assays. They also treat the physiological roles of lipases in normal and disordered metabolism, as well as strategies to target lipases for the treatment of diabetes, obesity and related disorders. Additional topics include the application of phospholipases for liposome-based drug delivery and their use as diagnostic tools.

Methods in Molecular Biology: Lipase and phospholipase protocols

Author : John M. Walker
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (84 download)

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Book Synopsis Methods in Molecular Biology: Lipase and phospholipase protocols by : John M. Walker

Download or read book Methods in Molecular Biology: Lipase and phospholipase protocols written by John M. Walker and published by . This book was released on 1984 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Lipolytic Enzymes

Author : Hans Brockerhoff
Publisher : Elsevier
ISBN 13 : 0323155162
Total Pages : 343 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Lipolytic Enzymes by : Hans Brockerhoff

Download or read book Lipolytic Enzymes written by Hans Brockerhoff and published by Elsevier. This book was released on 2012-12-02 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipolytic Enzymes focuses on the biochemistry of lipolytic enzymes, particularly, pancreatic lipase and phospholipase 2 as well as their structure and catalytic mechanism. It explores the kinetics of lipolysis, the digestive lipases of nonmammalian animals, the assay and purification of cholesterol esterases and phospholipases, the method phospholipases use in hydrolyzing phospholipids, and the adaptive mechanism of lipolytic enzymes at the lipid-water interface. Organized into eight chapters, this book begins with an overview of the importance of lipolytic enzymes, including their medical, therapeutic, food, and other industrial applications. It then proceeds with a discussion on the classification of lipolytic enzymes according to the type of bond they hydrolyze and the substrates on which they act. The next chapters look at the substrates and supersubstrates of lipolytic enzymes, along with their maximal velocity and the Michaelis constant. Moreover, the book talks about the detection and assay of lipases, the molecular properties of pancreatic cholesterol esterases, the stimulating effect of bile salts on cholesterol esterases, the hydrolytic cleavage of carboxyl esterases, and the occurrence and distribution of phosphohydrolases. A chapter discussing the two groups of lipolytic enzymes (the first containing enzymes of broad substrate specificity and not requiring cofactors; the second containing metalloenzymes with very narrow substrate requirements) concludes this book. This book is a valuable resource for chemists, biochemists, and those working in the field of nutritional sciences.

Esterases, Lipases, and Phospholipases

Author : M.I. Mackness
Publisher : Springer
ISBN 13 : 9781489909947
Total Pages : 279 pages
Book Rating : 4.9/5 (99 download)

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Book Synopsis Esterases, Lipases, and Phospholipases by : M.I. Mackness

Download or read book Esterases, Lipases, and Phospholipases written by M.I. Mackness and published by Springer. This book was released on 2013-06-06 with total page 279 pages. Available in PDF, EPUB and Kindle. Book excerpt: The NATO-Advanced Research Workshop "Esterases, Lipases and Phospholipases: From Structure to Clinical Significance" was held at the University of Bordeaux II, France from 22nd- 24th September 1993 under the Directorship of Professor Michel Clerc of the University of Bordeaux II. The meeting was organised by Hugues Chap (INSERM U 326, Toulouse, France), Georges Ferard (University of Strasbourg, France), Wolfgang Junge (University of Kiel, Germany) and Michael Mackness (University of Manchester, UK). In recent years it has become increasingly apparent that hydrolytic enzymes of the esterase, lipase and phospholipase type play central roles in the pathophysiology of many human diseases. The purpose of this NATO-ARW was to bring together experts (both clinical and scientific) in all three interrelated fields to review the current basic and clinical position and discuss future developments particularly with respect to future research aimed at determining the basic biochemical lesion involving hydrolytic enzymes involved in human disease and the use of these enzymes in diagnosis. As well as formal lectures from established researchers, the meeting also involved a number of lively round-table discussions on future developments and presentations from younger research workers, all of which are recorded in this Proceedings and which contribute to the success of the meeting.

Lipase

Author : Hamdi Sassi
Publisher : Nova Science Publishers
ISBN 13 : 9781620813942
Total Pages : 235 pages
Book Rating : 4.8/5 (139 download)

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Book Synopsis Lipase by : Hamdi Sassi

Download or read book Lipase written by Hamdi Sassi and published by Nova Science Publishers. This book was released on 2012-08-01 with total page 235 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lipases are ubiquitous enzymes which catalyze the hydrolysis of triacylglycerols to glycerols and free fatty acids. Due to their characteristics of enantioselectivity and regioselectivity and stability in organic solvents, they have gained importance in industrial applications. In this book, the authors examine the functions, synthesis and role in disease of lipases. Topics discussed include the action of lipases as a biotechnological tool; fungal lipases in biocatalysis; secreted phospholipase A2 inhibitors from cynara cardunculus L. and aloe vera extract as a potential therapeutic drug for inflammatory diseases; lipoprotein lipase activity in sickle cell nephropathy; lipases in the pharmaceutical industry; and yeast cell surface display of lipases.

Plant Lipid Metabolism

Author : J.C. Kader
Publisher : Springer Science & Business Media
ISBN 13 : 9401583943
Total Pages : 598 pages
Book Rating : 4.4/5 (15 download)

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Book Synopsis Plant Lipid Metabolism by : J.C. Kader

Download or read book Plant Lipid Metabolism written by J.C. Kader and published by Springer Science & Business Media. This book was released on 2013-04-18 with total page 598 pages. Available in PDF, EPUB and Kindle. Book excerpt: A collection of papers that comprehensively describe the major areas of research on lipid metabolism of plants. State-of-the-art knowledge about research on fatty acid and glycerolipid biosynthesis, isoprenoid metabolism, membrane structure and organization, lipid oxidation and degradation, lipids as intracellular and extracellular messengers, lipids and environment, oil seeds and gene technology is reviewed. The different topics covered show that modern tools of plant cellular and molecular biology, as well as molecular genetics, have been recently used to characterize several key enzymes of plant lipid metabolism (in particular, desaturases, thioesterases, fatty acid synthetase) and to isolate corresponding cDNAs and genomic clones, allowing the use of genetic engineering methods to modify the composition of membranes or storage lipids. These findings open fascinating perspectives, both for establishing the roles of lipids in membrane function and intracellular signalling and for adapting the composition of seed oil to the industrial needs. This book will be a good reference source for research scientists, advanced students and industrialists wishing to follow the considerable progress made in recent years on plant lipid metabolism and to envision the new opportunities offered by genetic engineering for the development of novel oil seeds.

Enzymes in Lipid Modification

Author : Uwe Theo Bornscheuer
Publisher : Wiley-Blackwell
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Enzymes in Lipid Modification by : Uwe Theo Bornscheuer

Download or read book Enzymes in Lipid Modification written by Uwe Theo Bornscheuer and published by Wiley-Blackwell. This book was released on 2000-09-13 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: Enzymatic methods of lipid modification, particularly of fats and oils, have developed rapidly since the 1980s. In parallel to the rapid progress in research a wide range of applications have emerged, e.g. in the food industry. The book is written by leading experts in the field and reflects the state-of-the-art of enzymatic lipid modification. It provides the reader with guidelines how to select suitable enzymes and how to apply them efficiently. Applications of lipases and phospholipases, lipoxygenases and P450-monooxygenases and the use of whole-cell systems in lipid modification are described. Cloning, expression and mutagenesis as well as attempts to understand the molecular basis of specificity and stereoselectivity are outlined. In addition engineering aspects and the choice of solvent systems are addressed.

Interfacial Phenomena

Author : J.T. Davies
Publisher : Academic Press
ISBN 13 : 0323148344
Total Pages : 507 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Interfacial Phenomena by : J.T. Davies

Download or read book Interfacial Phenomena written by J.T. Davies and published by Academic Press. This book was released on 2012-12-02 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: Interfacial Phenomena examines the fundamental properties of various liquid interfaces. This book discusses the physics of surfaces; electrostatic and electrokinetic phenomena; and adsorption at liquid interfaces. The properties of monolayers; reactions at liquid surfaces; diffusion through interfaces; and disperse systems and adhesion are also deliberated. Other topics include the vapor pressures over curved surfaces; electrical capacity of the double layer; applications of electrophoresis; and thermodynamics of adsorption and desorption. The experimental methods of spreading films at the oil-water interface; penetration into monolayers; experiments on dynamic systems; and spontaneous emulsification are likewise covered in this text. This book is beneficial to chemical engineers and students concerned with interfacial phenomena.

Microbial Enzymes: Roles and Applications in Industries

Author : Naveen Kumar Arora
Publisher : Springer Nature
ISBN 13 : 981151710X
Total Pages : 331 pages
Book Rating : 4.8/5 (115 download)

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Book Synopsis Microbial Enzymes: Roles and Applications in Industries by : Naveen Kumar Arora

Download or read book Microbial Enzymes: Roles and Applications in Industries written by Naveen Kumar Arora and published by Springer Nature. This book was released on 2020-04-28 with total page 331 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Microbial Enzymes: Roles and applications in industry” offers an essential update on the field of microbial biotechnology, and presents the latest information on a range of microbial enzymes such as fructosyltransferase, laccases, amylases, lipase, and cholesterol oxidase, as well as their potential applications in various industries. Production and optimisation technologies for several industrially relevant microbial enzymes are also addressed. In recent years, genetic engineering has opened up new possibilities for redesigning microbial enzymes that are useful in multiple industries, an aspect that the book explores. In addition, it demonstrates how some of the emerging issues in the fields of agriculture, environment and human health can be resolved with the aid of green technologies based on microbial enzymes. The topics covered here will not only provide a better understanding of the commercial applications of microbial enzymes, but also outline futuristic approaches to use microbial enzymes as driver of industrial sustainability. Lastly, the book is intended to provide readers with an overview of recent applications of microbial enzymes in various industrial sectors, and to pique researchers’ interest in the development of novel microbial enzyme technologies to meet the changing needs of industry.

The Impact of Food Bioactives on Health

Author : Kitty Verhoeckx
Publisher : Springer
ISBN 13 : 3319161040
Total Pages : 341 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis The Impact of Food Bioactives on Health by : Kitty Verhoeckx

Download or read book The Impact of Food Bioactives on Health written by Kitty Verhoeckx and published by Springer. This book was released on 2015-04-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.

The Chloroplast

Author : Constantin A. Rebeiz
Publisher : Springer Science & Business Media
ISBN 13 : 9048185319
Total Pages : 453 pages
Book Rating : 4.0/5 (481 download)

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Book Synopsis The Chloroplast by : Constantin A. Rebeiz

Download or read book The Chloroplast written by Constantin A. Rebeiz and published by Springer Science & Business Media. This book was released on 2010-07-15 with total page 453 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the industrial revolution that has been based on by higher photosynthetic efficiencies and more utilization of fossil fuels nears its end [R. A. Ker biomass production per unit area. (2007) Even oil optimists expect energy demand to According to Times Magazine (April 30, 2007 outstrip supply. Science 317: 437], the next indus- issue), one fifth of the US corn crop is presently trial revolution will most likely need development converted into ethanol, which is considered to burn of alternate sources of clean energy. In addition cleaner than gasoline and to produce less gre- to the development of hydroelectric power, these house gases. In order to meet a target of 35 billion efforts will probably include the conversion of gallons of ethanol produced by the year 2017, the wind, sea wave motion and solar energy [Solar Day entire US corn crop would need to be turned into in the Sun (2007) Business week, October 15, pp fuel. But crops such as corn and sugarcane cannot 69–76] into electrical energy. The most promising yield enough to produce all the needed fuel. F- of those will probably be based on the full usage thermore, even if all available starch is converted of solar energy. The latter is likely to be plenti- into fuel, it would only produce about 10% of ful for the next 2–3 billion years. Most probably, our gasoline needs [R. F.

Fungal Metabolites

Author : Jean-Michel Mérillon
Publisher :
ISBN 13 : 9783319194561
Total Pages : pages
Book Rating : 4.1/5 (945 download)

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Book Synopsis Fungal Metabolites by : Jean-Michel Mérillon

Download or read book Fungal Metabolites written by Jean-Michel Mérillon and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Intestinal Lipid Metabolism

Author : Charles M. Mansbach
Publisher : Springer Science & Business Media
ISBN 13 : 9780306462412
Total Pages : 464 pages
Book Rating : 4.4/5 (624 download)

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Book Synopsis Intestinal Lipid Metabolism by : Charles M. Mansbach

Download or read book Intestinal Lipid Metabolism written by Charles M. Mansbach and published by Springer Science & Business Media. This book was released on 2000-11-30 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book was stimulated by the enthusiasm shown by attendees at the meetings in Saxon River, VT, sponsored by the Federation ofAmerican Societies for Experimental Biology (FASEB), on the subject of the intestinal processing of lipids. When these meetings were first started in 1990, the original organizers, two of whom are editors ofthis volume (CMM and PT), had two major goals. The first was to bring together a diverse group ofinvestiga tors who had the common goal of gaining a better understanding of how the intestine ab sorbs lipids. The second was to stimulate the interest of younger individuals whom we wished to recruit into what we believed was an exciting and fruitful area ofresearch. Since that time, the field has opened up considerably with new questions being asked and new an swers obtained, suggesting that our original goals for the meetings were being met. In the same spirit, it occurred to us that there has not been a recentbook that draws to gethermuch ofthe informationavailableconcerninghow the intestineprocesses lipids. This book is intended to reach investigators with an interest in this area and their pre- and post doctoral students. The chapters are written by individuals who have a long-term interest in the areas about which they write, and many have been speakers at the subsequent FASEB conferences that have followed on the first.

Current Developments in Biotechnology and Bioengineering

Author : Amit Kumar Rai
Publisher : Elsevier
ISBN 13 : 0128236434
Total Pages : 463 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Current Developments in Biotechnology and Bioengineering by : Amit Kumar Rai

Download or read book Current Developments in Biotechnology and Bioengineering written by Amit Kumar Rai and published by Elsevier. This book was released on 2021-11-30 with total page 463 pages. Available in PDF, EPUB and Kindle. Book excerpt: Approx.438 pages Approx.438 pages

Handbook of Dairy Foods Analysis

Author : Fidel Toldrá
Publisher : CRC Press
ISBN 13 : 1000342034
Total Pages : 1041 pages
Book Rating : 4.0/5 (3 download)

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Book Synopsis Handbook of Dairy Foods Analysis by : Fidel Toldrá

Download or read book Handbook of Dairy Foods Analysis written by Fidel Toldrá and published by CRC Press. This book was released on 2021-03-29 with total page 1041 pages. Available in PDF, EPUB and Kindle. Book excerpt: Dairy foods account for a large portion of the Western diet, but due to the potential diversity of their sources, this food group often poses a challenge for food scientists and their research efforts. Bringing together the foremost minds in dairy research, Handbook of Dairy Foods Analysis, Second Edition, compiles the top dairy analysis techniques and methodologies from around the world into one well-organized volume. Exceptionally comprehensive in both its detailing of methods and the range of dairy products covered, this handbook includes tools for analyzing chemical and biochemical compounds and also bioactive peptides, prebiotics, and probiotics. It describes noninvasive chemical and physical sensors and starter cultures used in quality control. This second edition includes four brand-new chapters covering the analytical techniques and methodologies for determining bioactive peptides, preservatives, activity of endogenous enzymes, and sensory perception of dairy foods, and all other chapters have been adapted to recent research. All other chapters have been thoroughly updated. Key Features: Explains analytical tools available for the analysis of the chemistry and biochemistry of dairy foods Covers a variety of dairy foods including milk, cheese, butter, yogurt, and ice cream Analysis of nutritional quality includes prebiotics, probiotics, essential amino acids, bioactive peptides, and healthy vegetable-origin compounds Includes a series of chapters on analyzing sensory qualities, including color, texture, and flavor. Covering the gamut of dairy analysis techniques, th e book discusses current methods for the analysis of chemical and nutritional compounds, and the detection of microorganisms, allergens, contaminants, and/or other adulterations, including those of environmental origin or introduced during processing. Other methodologies used to evaluate color, texture, and flavor are also discussed. Written by an international panel of distinguished contributors under the editorial guidance of renowned authorities, Fidel Toldrá and Leo M.L. Nollet, this handbook is one of the few references that is completely devoted to dairy food analysis – an extremely valuable reference for those in the dairy research, processing, and manufacturing industries.