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Author : Jonathan M. Rhodes
Publisher :
ISBN 13 : 9781592595938
Total Pages : 616 pages
Book Rating : 4.5/5 (959 download)
Download or read book Lectin Methods and Protocols written by Jonathan M. Rhodes and published by . This book was released on 1998 with total page 616 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jun Hirabayashi
Publisher : Humana
ISBN 13 : 9781493954667
Total Pages : 0 pages
Book Rating : 4.9/5 (546 download)
Download or read book Lectins written by Jun Hirabayashi and published by Humana. This book was released on 2016-09-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lectins: Methods and Protocols summarizes classic lectin technologies and advanced techniques with high throughputs and sensitivities. Chapters include methods and techniques for serial lectin-affinity chromatography procedure, lectin-probed western blot and histochemical analyses, quantitative interaction analyses based on equilibrium dialysis, isothermal calorimetry, surface plasmon resonance, techniques for elucidating functions of endogenous animal lectins, advanced methods on “engineer” novel lectins by evolutionary concepts, and special equipment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Lectins: Methods and Protocols seeks to aid not only lectin specialists but also non-experts including both young scientists.
Author : Jun Hirabayashi
Publisher : Humana
ISBN 13 : 9781071604298
Total Pages : 0 pages
Book Rating : 4.6/5 (42 download)
Download or read book Lectin Purification and Analysis written by Jun Hirabayashi and published by Humana. This book was released on 2020-04-18 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents a series of experimental protocols for glycan analysis using lectins and the functional analysis of lectins. A key feature in this book are two comprehensive overview chapters on lectin structures and a list of the all the lectins—these chapters discuss the latest findings in the field. The remainder of the book covers topics such as lectins from mammals, non-mammalian animals; plants, algae, fungi, protists, bacteria, and viruses. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and thorough, Lectin Purification and Analysis: Methods and Protocols is a valuable tool for any scientist interested in learning more about these life systems.
Author : Robert S. Matson
Publisher : CRC Press
ISBN 13 : 1420046667
Total Pages : 216 pages
Book Rating : 4.4/5 (2 download)
Download or read book Microarray Methods and Protocols written by Robert S. Matson and published by CRC Press. This book was released on 2009-01-20 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Step-by-Step Guide to Present and Future Uses of Microarray TechnologyMicroarray technology continues to evolve, taking on a variety of forms. From the spotting of cDNA and the in situ synthesis of oligonucleotide arrays now come microarrays comprising proteins, carbohydrates, drugs, tissues, and cells. With contributions from microarray experts
Author : Anthony P. Corfield
Publisher : Springer Science & Business Media
ISBN 13 : 1592590489
Total Pages : 491 pages
Book Rating : 4.5/5 (925 download)
Download or read book Glycoprotein Methods and Protocols written by Anthony P. Corfield and published by Springer Science & Business Media. This book was released on 2007-10-26 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: The mucins (mucus glycoproteins) have long been a complex corner of glycoprotein biology. While dramatic advances in the separation, structural an- ysis, biosynthesis, and degradation have marked the progress in general glycop- tein understanding, the mucins have lagged behind. The reasons for this lack of progress have always been clear and are only now being resolved. The mucins are very large molecules; they are difficult to separate from other molecules present in mucosal secretions or membranes; they are often degraded owing to natural protective functions or to isolation methodology and their peptide and oligos- charide structures are varied and complex. Understanding these molecules has demanded progress in several major areas. Isolation techniques that protect the intact mucins and allow dissociation from other adsorbed but discrete molecules needed to be developed and accepted by all researchers in the field. Improved methods for the study of very large molecules with regard to their aggregation and polymerization were also needed. Structural analysis of the peptide domains and the multitude of oligosaccharide chains was required for smaller sample sizes, for multiple samples, and in shorter time. In view of these problems it is perhaps not surprising that the mucins have remained a dilemma, of obvious biological importance and interest, but very difficult to analyze.
Author : Michael L. Cher
Publisher : Springer Science & Business Media
ISBN 13 : 030648143X
Total Pages : 390 pages
Book Rating : 4.3/5 (64 download)
Download or read book Prostate Cancer: New Horizons in Research and Treatment written by Michael L. Cher and published by Springer Science & Business Media. This book was released on 2006-04-11 with total page 390 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years the pace of research in prostate cancer has increased dramatically. Creative ideas in combination with new and emerging technologies have led to an explosion of discovery. These types of advances in prostate cancer research presage an era of new treatment strategies based on an understanding of the cellular and molecular mechanisms of disease. In creating this book, we aimed to cover a broad "bench to bedside" research spectrum ranging from: genetic, molecular and cellular analyses to epidemiological studies, refinements in local treatment strategies and new biologically based non-hormonal treatments for systemic disease. Researchers and clinicians will find in this book a group of timely and clinically relevant chapters on prostate cancer research and treatment.
Author : Nathan Sharon
Publisher : Springer Science & Business Media
ISBN 13 : 1402069537
Total Pages : 464 pages
Book Rating : 4.4/5 (2 download)
Download or read book Lectins written by Nathan Sharon and published by Springer Science & Business Media. This book was released on 2007-11-03 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Second Edition brings readers up to date with all the latest findings in lectin research. Throughout this new edition, more than 200 figures and some thirty tables help readers visualize and understand key concepts and processes. The book starts with an overview of lectin research followed by a survey of the occurrence of lectins in nature. Other areas covered include the nutritional effects of lectins and their functions in nature.
Author : Stephen M. Palfrey
Publisher : Springer Science & Business Media
ISBN 13 : 1592596894
Total Pages : 215 pages
Book Rating : 4.5/5 (925 download)
Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.
Author : Swapna Thacheril Sukumaran
Publisher : Springer Nature
ISBN 13 : 9811551367
Total Pages : 581 pages
Book Rating : 4.8/5 (115 download)
Download or read book Plant Metabolites: Methods, Applications and Prospects written by Swapna Thacheril Sukumaran and published by Springer Nature. This book was released on 2020-11-28 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: Food security and the medicinal needs of billions of people around the world are pressing global issues, and the biodiversity and sustainable utilization of plants is of great significance in this context. Further, ethnobotanical studies are vital in the discovery of new drugs from indigenous medicinal plants, and plants with industrially important metabolites need to be cultivated to meet the growing market demand. In addition, the production of plant metabolites under in vitro conditions also has tremendous possibilities. The totipotency of plant cells plays a valuable role in the sustainable utilization of plant resources through cell, tissue and organ culture. At the same time, production can be enhanced using productive cell lines, treatment with elicitors, changing nutritional parameters and metabolic engineering. This book provides state-of-the-art information on biodiversity, conservation, ethnobotany, various aspects of In vitro secondary metabolite production, bioprospecting from various plant groups and drug discovery. It also discusses methods of extracting and characterizing drug leads from plant sources.
Author : Rosanna Peeling
Publisher : Springer Science & Business Media
ISBN 13 : 1592592120
Total Pages : 244 pages
Book Rating : 4.5/5 (925 download)
Download or read book Sexually Transmitted Diseases written by Rosanna Peeling and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years, molecular techniques have enhanced our ability to detect sexually transmitted infections and to conduct research to further our und- standing of sexually transmitted diseases. Molecular methods to quantitate pathogen load have also been shown to be useful for the management of HIV and other viral STDs. Existing laboratory manuals for the clinical microbi- ogy laboratory often do not include molecular methods for STDs. Sexually Transmitted Diseases: Methods and Protocols is intended to fill the need for a dedicated manual that covers all the fundamental aspects of molecular pro- cols for laboratory diagnosis, as well as research methodology for STDs, - cluding HIV. There are more than types of molecular techniques described in this book covering nine major sexually transmitted pathogens. Although mole- lar methods for the detection of such pathogens as Trichomonas vaginalis are available in the published literature, they have not been included since they are not yet widely used for laboratory diagnosis or research. Sexually Transmitted Diseases: Methods and Protocols is one of a series of books treating Methods Molecular Medicine, published by Humana Press.
Author : Sean R. Stowell
Publisher :
ISBN 13 : 9781071620557
Total Pages : 730 pages
Book Rating : 4.6/5 (25 download)
Download or read book Galectins written by Sean R. Stowell and published by . This book was released on 2022 with total page 730 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition is dedicated to new and updated methodological approaches designed to study galectin function. Chapters examine salient features of galectin functions. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Galectins: Methods and Protocols, Second Eidtion aims to be a useful practical guide to researches to help further their study in this field. .
Author : Carlo Pellicciari
Publisher : Humana
ISBN 13 : 9781071626771
Total Pages : 0 pages
Book Rating : 4.6/5 (267 download)
Download or read book Histochemistry of Single Molecules written by Carlo Pellicciari and published by Humana. This book was released on 2023-10-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details histochemical techniques for the detection of specific molecules or metabolic processes, both at light and electron microscopy. Chapters are divided into seven sections covering Vital histochemistry, Carbohydrate histochemistry, Protein histochemistry, Lipid histochemistry, Nuclear histochemistry, Plant histochemistry and Histochemistry for Nanoscience. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. The volume also contains three discursive chapters on Histochemistry in advanced cytometry, Lectins and Detection of molecules in plant cell walls by fluorescence microscopy. Authoritative and cutting-edge, Histochemistry of Single Molecules: Methods and Protocols, Second Edition aims to be a useful practical guide for researchers to help further their study in this field.
Author : Robert Brown
Publisher : Springer Science & Business Media
ISBN 13 : 1592596878
Total Pages : 241 pages
Book Rating : 4.5/5 (925 download)
Download or read book Cytotoxic Drug Resistance Mechanisms written by Robert Brown and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: There is now a range of cytotoxic drugs that have considerable clinical usefulness in producing responses in tumors and even, in a small proportion of cases, cure. However, the acquisition of drug resistance is a major clinical problem and is perhaps the main limiting factor in successful treatment of cancer. Thus, a tumor initially sensitive to chemotherapy will, in the majority of cases, eventually recur as a resistant tumor, which will then progress. Much of our understanding of drug resistance mechanisms comes from the study of tumor cell lines grown in tissue culture. We now understand many of the - lecular mechanisms that can lead to a cell acquiring resistance to antic- cer drugs; however, we still do not know which mechanism(s) are those most relevant to the problem of clinical drug resistance. Indeed, given that many of the cytotoxic anticancer drugs were discovered by random screening, it is - clear what features give a clinically useful anticancer drug a sufficient the- peutic index to be of value. The aim of Cytotoxic Drug Resistance Mechanisms is to provide pro- cols that are appropriate for examining the mechanisms of cellular resistance to anticancer cytotoxics in human tumor samples. Tumor cell lines have been enormously useful as experimental models of drug resistance mechanisms, however they have limitations and we need to address the relevance of such mechanisms in patients’ tumors. Examining drug resistance in tumors is much more problematic than in cell lines.
Author : Michelle Kilcoyne
Publisher : Academic Press
ISBN 13 : 0128220023
Total Pages : 464 pages
Book Rating : 4.1/5 (282 download)
Download or read book Translational Glycobiology in Human Health and Disease written by Michelle Kilcoyne and published by Academic Press. This book was released on 2023-11-29 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Along with nucleic acids, proteins, and lipids, carbohydrates stand as one of four main components of cellular architecture. However, glycobiology (or carbohydrate bioscience) is little understood by non-experts, partly because carbohydrates are a complex, diverse class of molecules structurally and functionally. In recent years, advances in computational analytics (glycomics) have allowed us to better interpret and realize the importance of glycobiology in human health and disease, and glycans and their associated processes have been shown to play a significant role across a variety of disease types. As the biomedical sciences continue to adopt multi-omic and precision medicine approaches, a greater understanding of glycobiology is essential for maintaining healthy physiology and advancing disease treatment.Translational Glycobiology in Human Health and Disease offers a deep examination of glycobiology for experts and non-experts alike in areas ranging from the role of glycobiology in chronic and infectious diseases to advances in technologies for higher throughput analysis and diagnosis. While keeping human health in the forefront, this book integrates a thorough discussion of glycobiology fundamentals with its growing areas of application and societal impact. With emphasis throughout on the interdisciplinary nature of glycosciences, this book also features perspectives from the health, computational (glycoanalytics), materials, biopharmaceutical, and diagnostic sciences.Disease and speciality areas addressed include gycoimmunology, neuroglycobiology, commensal glycobiology, gut health, regenerative medicine and glycobiology, glycobiology and cancer, congenital disorders of glycosylation, infectious disease glycobiology, and parasite glycobiology. Computational approaches discussed, supporting the advance of new research, include advanced glycoanalytics, glycomics microarrays, glycoengineering, and glycol systems biology. Additionally, authors consider impact areas for society and public health, such as glycobiology and entrepreneurship, policy and regulatory requirements for glycosylation, future research, and translation to new diagnostics and drug discovery. Provides a deep, foundational overview of glycoscience and its translational potential, highlighting glycobiology’s growing role in human health and disease study Examines a broad range of relevant disease areas and applications of glycobiology in policy and public health Features chapter contributions from leading, international experts in the field, fully integrating perspectives from the health, computational, materials, biopharmaceutical, and diagnostic sciences
Author : Sinéad T Loughran
Publisher : Springer Nature
ISBN 13 : 1071633627
Total Pages : 495 pages
Book Rating : 4.0/5 (716 download)
Download or read book Protein Chromatography written by Sinéad T Loughran and published by Springer Nature. This book was released on 2023-10-01 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition expands on the previous editions with updated and new chapters on protein chromatography. Chapters detail protein stability and storage, avoiding proteolysis, protein quantitation methods, generation and purification of recombinant proteins, recombinant antibody production, and the tagging of proteins. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Protein Chromatography: Methods and Protocols, Third Edition aims to provide commonly used methods and new approaches to help both new researchers and experts expand their knowledge.
Author : John M. Walker
Publisher : Springer
ISBN 13 : 1592591698
Total Pages : 1146 pages
Book Rating : 4.5/5 (925 download)
Download or read book The Protein Protocols Handbook written by John M. Walker and published by Springer. This book was released on 2007-10-02 with total page 1146 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Protein Protocols Handbook, Second Edition aims to provide a cross-section of analytical techniques commonly used for proteins and peptides, thus providing a benchtop manual and guide for those who are new to the protein chemistry laboratory and for those more established workers who wish to use a technique for the first time. All chapters are written in the same format as that used in the Methods in Molecular BiologyTM series. Each chapter opens with a description of the basic theory behind the method being described. The Materials section lists all the chemicals, reagents, buffers, and other materials necessary for carrying out the protocol. Since the principal goal of the book is to provide experimentalists with a full account of the practical steps necessary for carrying out each protocol successfully, the Methods section contains detailed st- by-step descriptions of every protocol that should result in the successful execution of each method. The Notes section complements the Methods material by indicating how best to deal with any problem or difficulty that may arise when using a given technique, and how to go about making the widest variety of modifications or alterations to the protocol. Since the first edition of this book was published in 1996 there have, of course, been significant developments in the field of protein chemistry.
Author : Nicolle H. Packer
Publisher : Humana Press
ISBN 13 : 9781617377693
Total Pages : 0 pages
Book Rating : 4.3/5 (776 download)
Download or read book Glycomics written by Nicolle H. Packer and published by Humana Press. This book was released on 2010-11-19 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Due to the significant contributions of carbohydrates to the functional diversity of the cell, the challenging study of the glycome has expanded beyond the research of carbohydrate experts and into the wider scope of the life sciences. To aid all scientists now delving into this vital subject area, Glycomics: Methods and Protocols collects a compendium of detailed laboratory protocols reflecting the increasing availability of sample preparation, chromatographic, electrophoretic, mass spectrometric, and bioinformatic tools specifically designed for the analysis of glycosylation. Leading researchers in the field address subjects such as glycoprotein and proteoglycan analysis, glycosylation structure determination, as well as various approaches to investigate the interaction between glycans and a variety of carbohydrate-recognizing proteins in order to aid exploration into the functional significance of the oligosaccharides. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Glycomics: Methods and Protocols serves as a valuable guide for experimenters facing the challenges of glycan analysis in hope of providing further insights into the biology of cell-cell communication and interaction.
