Laboratory Methods in Vesicular and Vectorial Transport

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Publisher : Academic Press
ISBN 13 : 0323146791
Total Pages : 537 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Laboratory Methods in Vesicular and Vectorial Transport by : Alan Tartakoff

Download or read book Laboratory Methods in Vesicular and Vectorial Transport written by Alan Tartakoff and published by Academic Press. This book was released on 2012-12-02 with total page 537 pages. Available in PDF, EPUB and Kindle. Book excerpt: Laboratory Methods in Vesicular and Vectorial Transport describes the procedures used to study the mechanisms of vesicular transport along the secretory and endocytic paths, including electron microscopy, autoradiography, and methods associated with cyto- and immunocytochemistry, genetics, and biochemistry. It investigates vectorial transport to the cisternal space of the rough endoplasmic reticulum (ER), as well as protein translocation across the ER, strategies for gaining access to the cytoplasm, cell-free analysis of vesicle fusion, the structure of glycoproteins, and the use of cell systems for analysis of vesicular traffic. Organized into seven parts encompassing 20 chapters, this volume begins with an overview of protein topology in the ER and the use of cross-linking methods to probe the molecular environment of translocating polypeptide chains. It then discusses the reconstitution of secretory protein translocation from detergent-solubilized rough microsomes; the use of anti-idiotype antibodies to characterize protein-protein interactions; the use of perforated cells to elucidate intracellular membrane transport; delivery of macromolecules into cells expressing a viral membrane fusion protein; and digitonin permeabilization procedures for studying endosome acidification and function. The reader is also introduced to reconstitution of intracellular vesicle fusion in a cell-free system after receptor-mediated endocytosis; immunoisolation using magnetic solid supports; endosome and lysosome purification by free-flow electrophoresis; remodeling of glycoprotein oligosaccharides after endocytosis; and replica plating of animal cells. This book will interest students, researchers, geneticists, biochemists, and cell biologists.

Vectorial Transport of Proteins into and across Membranes

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Publisher : Academic Press
ISBN 13 : 9780125641340
Total Pages : 426 pages
Book Rating : 4.6/5 (413 download)

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Book Synopsis Vectorial Transport of Proteins into and across Membranes by :

Download or read book Vectorial Transport of Proteins into and across Membranes written by and published by Academic Press. This book was released on 1991-09-20 with total page 426 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the third volume in this series covering protein translocation within and between cells. Volumes 31 and 32, Vesicular Transport, Parts A and B, treated techniques for eukaryotic cells available as of 1989. Vectorial Transport of Proteins into and across Membranes, brings together new methods and new topics as of 1991. Highlights include use of anti-idiotype antibodies, gene fusions, transcription, crosslinking, pulse-labeling, and reconstitution. Describes contemporary approaches for studying protein transport, protein topology and organelle biogenesis Covers methods from pre-eminent laboratories including: In vitro and in vivo investigations; Biochemical and immunological techniques; Prokaryote, chloroplast, mitochondria, peroxisome, and rough endoplasmic reticulum membranes

Laboratory Methods in Cell Biology: Imaging

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Publisher : Academic Press
ISBN 13 : 0124078079
Total Pages : 447 pages
Book Rating : 4.1/5 (24 download)

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Book Synopsis Laboratory Methods in Cell Biology: Imaging by :

Download or read book Laboratory Methods in Cell Biology: Imaging written by and published by Academic Press. This book was released on 2013-01-03 with total page 447 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell biology spans among the widest diversity of methods in the biological sciences. From physical chemistry to microscopy, cells have given up with secrets only when the questions are asked in the right way! This new volume of Methods in Cell Biology covers laboratory methods in cell biology, and includes methods that are among the most important and elucidating in the discipline, such as bioluminescent imaging of gene expressions, confocal imaging, and electron microscopy of bone. Covers the most important laboratory methods in cell biology Chapters written by experts in their fields

Methods in Muscle Biology

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Publisher : Academic Press
ISBN 13 : 0125641540
Total Pages : 521 pages
Book Rating : 4.1/5 (256 download)

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Book Synopsis Methods in Muscle Biology by : Charles Emerson

Download or read book Methods in Muscle Biology written by Charles Emerson and published by Academic Press. This book was released on 1997 with total page 521 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Muscle Biology is a comprehensive laboratory guide that details the methods used in the study of muscle biology. The techniques included embrace cell, developmental, and molecular biology, as well asphysiology, neurobiology, and medical research.

Animal Cell Culture Methods

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Publisher : Academic Press
ISBN 13 : 9780080859552
Total Pages : 368 pages
Book Rating : 4.8/5 (595 download)

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Book Synopsis Animal Cell Culture Methods by :

Download or read book Animal Cell Culture Methods written by and published by Academic Press. This book was released on 1998-07-03 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides complete and thorough coverage of the classical and state-of-the-art methods used in cell culture. It also includes basic principles used in the selection of cells for specific scientific study, as well as analytical and procedural techniques. Key Features * Reviews basic principles of cell culture * Gives options and techniques on how to look at cells

Methods in Avian Embryology

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Publisher : Academic Press
ISBN 13 : 9780080859491
Total Pages : 369 pages
Book Rating : 4.8/5 (594 download)

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Book Synopsis Methods in Avian Embryology by :

Download or read book Methods in Avian Embryology written by and published by Academic Press. This book was released on 1996-05-16 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: A current and authoritative guide, Methods in Avian Embryology presents a combination of classical embryological techniques and modern molecular biological approaches to studying the developing avian embryo. The only one of its kind, this book is specifically devoted to providing a detailed approach to studying avian embryos. It also describes how to use this system to study problems in cell, developmental, and neurobiology. The protocols emphasize microsurgery, histology, and cellular and molecular marking, which are not covered in the usual molecular biology methods manuals. The methods include: embryonic transplantations, cell culture and organ culture, in situ hybridization, classical histological techniques, and retrovirally mediated gene transfer. Key Features * Complete and easy-to-follow procedures * Helpful illustrations * Distinguished group of authors * Wide range of approaches

Computational Methods in Cell Biology

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Publisher : Academic Press
ISBN 13 : 0123884217
Total Pages : 427 pages
Book Rating : 4.1/5 (238 download)

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Book Synopsis Computational Methods in Cell Biology by :

Download or read book Computational Methods in Cell Biology written by and published by Academic Press. This book was released on 2012-05-31 with total page 427 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computational methods are playing an ever increasing role in cell biology. This volume of Methods in Cell Biology focuses on Computational Methods in Cell Biology and consists of two parts: (1) data extraction and analysis to distill models and mechanisms, and (2) developing and simulating models to make predictions and testable hypotheses. Focuses on computational methods in cell biology Split into 2 parts--data extraction and analysis to distill models and mechanisms, and developing and simulating models to make predictions and testable hypotheses Emphasizes the intimate and necessary connection with interpreting experimental data and proposing the next hypothesis and experiment

Methods in Nano Cell Biology

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Publisher : Academic Press
ISBN 13 : 008094910X
Total Pages : 534 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Methods in Nano Cell Biology by :

Download or read book Methods in Nano Cell Biology written by and published by Academic Press. This book was released on 2008-12-11 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Understanding live cells at the single molecule level is the most important and single major challenge facing biology and medicine today. Nanobiology focuses on the properties and structure of complex assemblies of biomolecules—biochips and molecular motors, for example—in conjunction with distinctive surfaces, rods, dots, and materials of nanoscience. Nano Cell Biology will describe the current applications of nanobiology to the study of the structure, function, and metabolic processes of cells. Provides historical background on this newly emerging field Covers the latest application of new instrumentation in the field Detailed protocols in the study of live cells at the nanometer level Discusses future technologies and their applications in the study of living cells

Methods in Cell-Matrix Adhesion

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Publisher : Academic Press
ISBN 13 : 0080524966
Total Pages : 461 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Methods in Cell-Matrix Adhesion by : Josephine Adams

Download or read book Methods in Cell-Matrix Adhesion written by Josephine Adams and published by Academic Press. This book was released on 2002-06-12 with total page 461 pages. Available in PDF, EPUB and Kindle. Book excerpt: Critically acclaimed for more than 25 years, the Methods in Cell Biology series provides an indispensable tool for the researcher. Each volume is carefully edited by experts to contain state-of-the-art reviews and step-by-step protocols. Techniques are described completely so that methods are made accessible to users. This volume, Methods of Cell-Matrix Adhesion, contains integrated coverage on cell-matrix adhesion methods. It brings the classical methodologies and the latest techniques together in one concise volume. This coverage includes experimental protocols and their conceptual background for all aspects of cell-matrix adhesion research: the extracellular matrix, adhesion receptors, and the growing number of functional applications of matrix-adhesion in molecular cell biology. Also covered is the purification of the extracellular matrix to functional analyses of cellular responses.

Methods in Plant Cell Biology

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Publisher : Academic Press
ISBN 13 : 0125641516
Total Pages : 605 pages
Book Rating : 4.1/5 (256 download)

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Book Synopsis Methods in Plant Cell Biology by : David W. Galbraith

Download or read book Methods in Plant Cell Biology written by David W. Galbraith and published by Academic Press. This book was released on 1995 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.

Methods in Plant Cell Biology

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Author :
Publisher : Academic Press
ISBN 13 : 9780080859484
Total Pages : 555 pages
Book Rating : 4.8/5 (594 download)

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Book Synopsis Methods in Plant Cell Biology by :

Download or read book Methods in Plant Cell Biology written by and published by Academic Press. This book was released on 1995-10-10 with total page 555 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. This is accompanied by examples of typical results, illustrations, troubleshooting of potential pitfalls, sources of chemicals and equipment, and complete reference lists. Protocols are written to be easily comprehended by beginning research students, but these extensive volumes will also be a valuable addition to the libraries of expert researchers. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Methods in Plant Cell Biology, Part A

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Author :
Publisher : Elsevier
ISBN 13 : 008085947X
Total Pages : 605 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Methods in Plant Cell Biology, Part A by :

Download or read book Methods in Plant Cell Biology, Part A written by and published by Elsevier. This book was released on 1995-10-10 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features* Written by experts, many of whom have developed the individual methods described* Contains most, if not all, the methods needed for modern research in plant cell biology* Up-to-date and comprehensive* Full references* Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures* Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants

Methods for Analysis of Golgi Complex Function

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Publisher : Elsevier
ISBN 13 : 0124171834
Total Pages : 499 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis Methods for Analysis of Golgi Complex Function by :

Download or read book Methods for Analysis of Golgi Complex Function written by and published by Elsevier. This book was released on 2013-11-26 with total page 499 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Cell Biology looks at methods for analyzing of golgi complex function. Chapters cover such topics as in vitro reconstitution systems, fluorescence-based analysis of trafficking in mammalian cells and high content screening. With cutting-edge material, this comprehensive collection is intended to guide researchers for years to come. Covers sections on model systems and functional studies, imaging-based approaches and emerging studies Chapters are written by experts in the field Cutting-edge material

Neurons: Methods and Applications for the Cell Biologist

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Publisher : Gulf Professional Publishing
ISBN 13 : 9780123525666
Total Pages : 482 pages
Book Rating : 4.5/5 (256 download)

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Book Synopsis Neurons: Methods and Applications for the Cell Biologist by : Peter J. Hollenbeck

Download or read book Neurons: Methods and Applications for the Cell Biologist written by Peter J. Hollenbeck and published by Gulf Professional Publishing. This book was released on 2003-07-08 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Many cell biologists study the properties of cells grown outside of the animal, in the controlled environment of a culture dish. Many cell biologists would benefit from directing their attention to nerve cells, but are afraid to try growing nerve cells in culture because it has the reputation for being much more difficult and unreliable than growing other kinds of cells. This volume is written by cell biologists and neuroscientists who are experts at growing various kinds of neurons in culture and using them for diverse experimental applications. The aim of this volume is to demystify the techniques used to grow nerve cells and to show cell biologists that it is something that they can easily master. * Contains culture methodology for more than a dozen types of CNS and PNS neurons * Includes most recent and reliable techniques from expert practitioners for specific experimental applications * Addresses the latest strategies for transfecting neurons.

Recent Advances in Cytometry, Part A

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Publisher : Academic Press
ISBN 13 : 0080957021
Total Pages : 600 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Recent Advances in Cytometry, Part A by :

Download or read book Recent Advances in Cytometry, Part A written by and published by Academic Press. This book was released on 2011-06-13 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cytometry is one of the most rapidly growing methodologies available for basic cell and molecular biology, cytogenetics, immunology, oncology, environmental sciences and also various fields of clinical medicine. This new edition, split into 2 Parts, is an almost completely new book, with nearly all of the chapters devoted to new topics. Like the previous volumes on cytometry published as part of the Methods in Cell Biology series, it provides a comprehensive description of particular cytometric methods and reviews their applications. Chapters present the theoretical foundations of the described methods, their applicability in experimental laboratory and clinical settings, and describes common traps and pitfalls such as problems with data interpretation, comparison with alternative assays, and choosing the optimal assay. Comprehensive presentation of cytometric methods covering theoretical applications, applicability, potential pitfalls, and comparisions to alternative assays Discusses many new assays developed since the previous edition Presents recent developments in cytometric intrumentation/technology

Biophysical Tools for Biologists

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Publisher : Academic Press
ISBN 13 : 0080919782
Total Pages : 704 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Biophysical Tools for Biologists by :

Download or read book Biophysical Tools for Biologists written by and published by Academic Press. This book was released on 2009-01-19 with total page 704 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vivo and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method

Biophysical Tools for Biologists

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Publisher : Academic Press
ISBN 13 : 0080554946
Total Pages : 996 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Biophysical Tools for Biologists by : John J. Correia

Download or read book Biophysical Tools for Biologists written by John J. Correia and published by Academic Press. This book was released on 2011-09-21 with total page 996 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven in part by the development of genomics, proteomics, and bioinformatics as new disciplines, there has been a tremendous resurgence of interest in physical methods to investigate macromolecular structure and function in the context of living cells. This volume in Methods in Cell Biology is devoted to biophysical techniques in vitro and their applications to cellular biology. Biophysical Tools for Biologists covers methods-oriented chapters on fundamental as well as cutting-edge techniques in molecular and cellular biophysics. This book is directed toward the broad audience of cell biologists, biophysicists, pharmacologists, and molecular biologists who employ classical and modern biophysical technologies or wish to expand their expertise to include such approaches. It will also interest the biomedical and biotechnology communities for biophysical characterization of drug formulations prior to FDA approval. Describes techniques in the context of important biological problems Delineates critical steps and potential pitfalls for each method Includes full-color plates to illustrate techniques