Isolated Hepatocytes

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The Isolated hepatocyte

Author : E.J. Rauckman
Publisher : Elsevier
ISBN 13 : 0323156797
Total Pages : 305 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis The Isolated hepatocyte by : E.J. Rauckman

Download or read book The Isolated hepatocyte written by E.J. Rauckman and published by Elsevier. This book was released on 2012-12-02 with total page 305 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Isolated Hepatocyte: Use in Toxicology and Xenobiotic Biotransformations covers the link between research on the isolated hepatocyte and the disciplines of cell culture, toxicology, metabolism, and molecular biology. This book is composed of 11 chapters and begins with an overview of the regulation of liver growth, sulfation, glucuronidation of xenobiotics in specific liver sites. The next chapters deal with toxicology studies in cultured hepatocytes from various species and the in vitro control of hepatocyte proliferation. These topics are followed by discussions of choices and methods of cytotoxicity measures; hepatoxicity of metals; the metabolism and toxicity of xenobiotics in a primary culture; and the mechanism of carcinogen-induced pleiotropic drug resistance in hepatocytes. The concluding chapters describe the in vivo and in vitro measurement of chemically-induced DNA repair in hepatocytes, as well as the genotoxicity studies with human hepatocytes. This book is intended primarily to toxicologists and researchers.

Isolated Hepatocytes: Preparation, Properties and Applications

Author : M.N. Berry
Publisher : Elsevier
ISBN 13 : 0080858902
Total Pages : 483 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Isolated Hepatocytes: Preparation, Properties and Applications by : M.N. Berry

Download or read book Isolated Hepatocytes: Preparation, Properties and Applications written by M.N. Berry and published by Elsevier. This book was released on 1991-09-30 with total page 483 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book sets out clearly and effectively the preparation and working methods of laboratory techniques involving isolated hepatocytes and will make life easier for every laboratory worker concerned with these techniques.

Isolated and Cultured Hepatocytes

Author : André Guillouzo
Publisher :
ISBN 13 :
Total Pages : 424 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Isolated and Cultured Hepatocytes by : André Guillouzo

Download or read book Isolated and Cultured Hepatocytes written by André Guillouzo and published by . This book was released on 1986 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt:

The Hepatocyte Review

Author : M. N. Berry
Publisher : Springer Science & Business Media
ISBN 13 : 9401733457
Total Pages : 597 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis The Hepatocyte Review by : M. N. Berry

Download or read book The Hepatocyte Review written by M. N. Berry and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 597 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is thirty years since the technique of high-yield preparation of isolated hepatocytes, by collagenase perfusion of the liver, was published. The original method described by Berry and Friend has undergone many minor modifications by other workers, and the two-step procedure introduced by Seglen in 1976 has become the most frequent way to prepare hepatocyte suspensions. An important development introduced by Bissell in 1973 was the use of the cells as the first step in monolayer culture. The availability of the isolated hepatocyte preparation as cells in suspension or culture has undoubtedly facilitated research on the liver. This was emphasised in our book, published (with Dr. Greg Barritt) in 1990, which described in detail methods of preparation and the properties of the isolated hepatocytes. It also discussed the usefulness of the preparation for the study of intermediary and xenobiotic metabolism, calcium ion transport, and the growth and differentiation of hepatocytes in culture. The book also touched briefly on a range of specialised techniques, including peri fusion, subcellular fractionation, transplantation, cryopreservation and measurement of intracellular pH. Although standard procedures for the manipulation of hepatocytes have not changed a great deal in ten years, they have undoubtedly been refined. This applies particularly to hepatocyte culture techniques, cryopreservation, and even to preparation of hepatocyte suspensions, where it is now feasible to use purified enzymes. There is also much more emphasis on the use and study of human hepatocytes, particularly in the field of pharmacology and therapeutics.

Isolated and Cultured Hepatocytes

Author : Christiane Guguen-Guillouzo
Publisher :
ISBN 13 : 9782855982892
Total Pages : 0 pages
Book Rating : 4.9/5 (828 download)

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Book Synopsis Isolated and Cultured Hepatocytes by : Christiane Guguen-Guillouzo

Download or read book Isolated and Cultured Hepatocytes written by Christiane Guguen-Guillouzo and published by . This book was released on 1986 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Density Separation of Intact Isolated Hepatocytes

Author : Gunnar Bengtsson
Publisher :
ISBN 13 :
Total Pages : 54 pages
Book Rating : 4.:/5 (35 download)

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Book Synopsis Density Separation of Intact Isolated Hepatocytes by : Gunnar Bengtsson

Download or read book Density Separation of Intact Isolated Hepatocytes written by Gunnar Bengtsson and published by . This book was released on 1983 with total page 54 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Isolation and Characterization of Ruminant Hepatocytes

Author : Mary Ellen Camille Looney
Publisher :
ISBN 13 :
Total Pages : 112 pages
Book Rating : 4.:/5 (15 download)

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Book Synopsis Isolation and Characterization of Ruminant Hepatocytes by : Mary Ellen Camille Looney

Download or read book Isolation and Characterization of Ruminant Hepatocytes written by Mary Ellen Camille Looney and published by . This book was released on 1981 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Isolated Hepatocytes

Author : Michael N. Berry
Publisher :
ISBN 13 :
Total Pages : 11 pages
Book Rating : 4.:/5 (893 download)

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Book Synopsis Isolated Hepatocytes by : Michael N. Berry

Download or read book Isolated Hepatocytes written by Michael N. Berry and published by . This book was released on with total page 11 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Isolated Hepatocytes

Author : Michael N. Berry
Publisher :
ISBN 13 : 9780720442007
Total Pages : 460 pages
Book Rating : 4.4/5 (42 download)

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Book Synopsis Isolated Hepatocytes by : Michael N. Berry

Download or read book Isolated Hepatocytes written by Michael N. Berry and published by . This book was released on 1991 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mouse Cell Culture

Author : Andrew Ward
Publisher : Humana Press
ISBN 13 : 9781588297723
Total Pages : 0 pages
Book Rating : 4.2/5 (977 download)

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Book Synopsis Mouse Cell Culture by : Andrew Ward

Download or read book Mouse Cell Culture written by Andrew Ward and published by Humana Press. This book was released on 2010-03-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cultured cells have combined accessibility and the ability to expand a homogeneous cell population from a relatively limited source, thus opening up a wealth of possibilities for researchers. In Mouse Cell Culture: Methods and Protocols, expert researchers provide a number of methods for the culture of a wide range of specific cells and tissues isolated from the key genetic model of the fetal or adult mouse. Including protocols for the explant of fetal tissues and stem cells that allow developmental processes to be followed ex vivo as well as protocols for the culture of isolated cell types that allow for the study of relatively homogeneous cell populations, this volume brings together a selection of the most current methods in order to make them available in one convenient source. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Practical and authoritative, Mouse Cell Culture: Methods and Protocols serves as an immediately applicable springboard for the development of new tissue culture methods in order to advance the study and treatment of human disorders.

Hepatocyte Transplantation

Author : S. Gupta
Publisher : Springer Science & Business Media
ISBN 13 : 9780792387763
Total Pages : 386 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Hepatocyte Transplantation by : S. Gupta

Download or read book Hepatocyte Transplantation written by S. Gupta and published by Springer Science & Business Media. This book was released on 2002-09-30 with total page 386 pages. Available in PDF, EPUB and Kindle. Book excerpt: In recent years there has been an increasing need for transplantation, but the number of donor livers available has increased only slightly, despite intensive public relations activities. New concepts in the field of transplantation, for instance the transplantation of living donor organs or the splitting of organs, are urgently required, to safeguard the treatment of patients with severe liver disease. The development and clinical application of cell therapy for patients with liver disease could soon present a significant enhancement of the therapeutic options. The aim of such cell therapy is to repair or improve the biological function of the chronically and acutely damaged liver. Even though systematic trials are not available, individual case reports and small series already show promising clinical results. Present concepts of cell therapy for liver diseases based on the use of primary hepatocytes have recently been considerably extended through new data on the biology of stem cells. The adult haematopoetic stem cell as a pool for hepatocyte grafts - what would be the perspectives for the clinical application? This book is the proceedings of the Falk Symposium No. 126 on `Hepatocyte Transplantation' (Progress in Gastroenterology and Hepatology Part III) held in Hannover, Germany, October 2-3, 2001, and is a forum for basic research, but also for questions concerning clinical applications in the field of hepatocyte transplantation.

Liver Cells and Drugs

Author : André Guillouzo
Publisher : John Libbey Eurotext
ISBN 13 : 9780861961283
Total Pages : 520 pages
Book Rating : 4.9/5 (612 download)

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Book Synopsis Liver Cells and Drugs by : André Guillouzo

Download or read book Liver Cells and Drugs written by André Guillouzo and published by John Libbey Eurotext. This book was released on 1988 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book contains the 17 lectures given at the International Symposium on Liver Cells and Drugs held in Rennes on 7 to 10 July 1987 as well as 50 communications presented at the poster sessions.

Comparison of Freshly Isolated Human Hepatocytes with Human Hepatocytes-like Cells

Author : Jing Yuan
Publisher :
ISBN 13 :
Total Pages : 150 pages
Book Rating : 4.:/5 (249 download)

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Book Synopsis Comparison of Freshly Isolated Human Hepatocytes with Human Hepatocytes-like Cells by : Jing Yuan

Download or read book Comparison of Freshly Isolated Human Hepatocytes with Human Hepatocytes-like Cells written by Jing Yuan and published by . This book was released on 2003 with total page 150 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Hepatocytes

Author : Patrick Maurel
Publisher : Humana Press
ISBN 13 : 9781607616870
Total Pages : 554 pages
Book Rating : 4.6/5 (168 download)

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Book Synopsis Hepatocytes by : Patrick Maurel

Download or read book Hepatocytes written by Patrick Maurel and published by Humana Press. This book was released on 2010-05-07 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hepatocytes account for approximately 80% of the liver mass and play a significant role in various aspects of liver physiopathology, exhibiting unrivaled complexity and diversity of functions. In Hepatocytes: Methods and Protocols, expert researchers provide the reader with methods, technical protocols, and review chapters focusing on selected areas of hepatocyte biology including isolation, culture, differentiation and stem cells, and hepatocyte use in clinical, basic, and applied research. With a specific emphasis on human hepatocytes, the volume presents chapters covering subjects including hepatocyte culture models, cryopreservation methods, differentiation assessment, liver ontogenesis, production of hepatocytes from stem cells, drug/xenobiotic metabolism, toxicity and transport, bile acid and blood coagulation factor production, infection by HBV and HCV, humanized animals, biortificial liver devices, hepatocyte transplantation. As a volume in the highly successful Methods in Molecular BiologyTM series, protocol chapters include brief introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Comprehensive and cutting-edge, Hepatocytes: Methods and Protocols will be useful to all those who are currently using or planning to use human, or animal, hepatocytes to investigate any aspect of liver physiopathology or who are interested in liver development or liver stem cells and liver biotherapy.

Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies

Author : John R. Williamson
Publisher :
ISBN 13 : 9780720445299
Total Pages : 500 pages
Book Rating : 4.4/5 (452 download)

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Book Synopsis Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies by : John R. Williamson

Download or read book Use of Isolated Liver Cells and Kidney Tubules in Metabolic Studies written by John R. Williamson and published by . This book was released on 1976 with total page 500 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Basic Cell Culture Protocols

Author : Cheryl D. Helgason
Publisher : Humana Press
ISBN 13 : 9781493956524
Total Pages : 550 pages
Book Rating : 4.9/5 (565 download)

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Book Synopsis Basic Cell Culture Protocols by : Cheryl D. Helgason

Download or read book Basic Cell Culture Protocols written by Cheryl D. Helgason and published by Humana Press. This book was released on 2016-08-23 with total page 550 pages. Available in PDF, EPUB and Kindle. Book excerpt: At some point in their careers, virtually every scientist and technician, as well as many medical professionals, regardless of their area of specialization have a need to utilize cell culture systems. Updating and significantly expanding upon the previous editions, Basic Cell Culture Protocols, Fourth Edition provides the novice cell culturist with sufficient information to perform the basic techniques, to ensure the health and identity of their cell lines, and to be able to isolate and culture specialized primary cell types. The intent of this extensive volume is to generate a valuable resource containing clear methodologies pertinent to current areas of investigation, rather than attempting to educate cell culturists on specific cell types or organ systems. Written in the highly successful Methods in Molecular BiologyTM, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Comprehensive and up-to-date, Basic Cell Culture Protocols, Fourth Edition compiles the essential techniques needed to approach this vital laboratory activity with full success.

Isolation and Differentiation of Adult and Embryonic Stem Cells Into Hepatocytes in Vitro

Author : Robert Edward Schwartz
Publisher :
ISBN 13 :
Total Pages : 456 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Isolation and Differentiation of Adult and Embryonic Stem Cells Into Hepatocytes in Vitro by : Robert Edward Schwartz

Download or read book Isolation and Differentiation of Adult and Embryonic Stem Cells Into Hepatocytes in Vitro written by Robert Edward Schwartz and published by . This book was released on 2005 with total page 456 pages. Available in PDF, EPUB and Kindle. Book excerpt: