Insights Of Mixing On The Assembly Of Dna Nanoparticles

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Insights of Mixing on the Assembly of DNA Nanoparticles

Author : Manda S. Williams
Publisher :
ISBN 13 :
Total Pages : 142 pages
Book Rating : 4.:/5 (88 download)

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Book Synopsis Insights of Mixing on the Assembly of DNA Nanoparticles by : Manda S. Williams

Download or read book Insights of Mixing on the Assembly of DNA Nanoparticles written by Manda S. Williams and published by . This book was released on 2013 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt: Size is a crucial parameter in the delivery of nanoparticle therapeutics, affecting mechanisms such as tissue delivery, clearance, and cellular uptake. The morphology of nanoparticles is dependent both upon chemistry and the physical process of assembly. Polyplexes, a major class of non-viral gene delivery vectors, are conventionally prepared by vortex mixing, resulting in non-uniform nanoparticles and poor reproducibility. Better understanding and control of the physical process of assembly, and mixing in particular, will produce polyplexes of a more uniform and reliable size, optimizing their efficiency for laboratory and clinical use. "Mixing" is the reduction of length scale of a system to accelerate diffusion until a uniform concentration is achieved. Vortex mixing is poorly characterized and sensitive to protocols. Microfluidic systems are notable for predictable fluid behavior, and are ideal for analyzing and controlling the physical interaction of reagents on the microscale, realm where mixing occurs. Several microdevices for the preparation of DNA polyplexes are explored here. Firstly, the staggered herringbone mixer, a chaotic advection micromixer, is used to observe the effects of mixing time on nanoparticle size. Next, a novel device to surround the reagent flows with a sheath of buffer, preventing interaction with the walls and confining the complexation to a zone of lower, less variable shear and residence time, is used to demonstrate the role of shear in nanoparticle assembly. Lastly, uneven diffusion between ion pairs produces a small separation of charge at fluid interfaces; this short-lived electric field has a significant impact on the transport of DNA over the time scales of mixing and complexation. The effects of common buffers on the transport of DNA are examined for possible applications to mixing and complexation. These three investigations demonstrate the importance of the physical process in polyplex assembly, and indicate several important considerations in the development of new protocols and devices.

DNA Nanotechnology

Author : Giampaolo Zuccheri
Publisher : Humana
ISBN 13 : 9781493961382
Total Pages : 0 pages
Book Rating : 4.9/5 (613 download)

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Book Synopsis DNA Nanotechnology by : Giampaolo Zuccheri

Download or read book DNA Nanotechnology written by Giampaolo Zuccheri and published by Humana. This book was released on 2016-08-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Currently, nanotechnology is exposing the properties of DNA in unprecedented detail leading to new insights on the biological behavior and function of DNA. With the structural perfection of a self-assembling DNA nano-object, such as a DNA origami, it is clear how complex DNA is as a molecule, leading researchers to wonder how many different constructs could be designed and realized. DNA Nanotechnology: Methods and Protocols shows the procedures to follow in order to repeat methods that lead to such constructs or to the mastering of the characterization techniques used to study them. The chapters of this book are roughly divided into two parts: some cover the methods for preparing the nanostructures, from the rationale of the operations to the techniques for their handling, while other chapters deal more directly with advanced instrumental techniques that can manipulate and characterize molecules and nanostructures. Written in the highly successful Methods in Molecular BiologyTM series format, chapters contain introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, DNA Nanotechnology: Methods and Protocols serves as an ideal guide to scientists of all backgrounds and aims to ignite interest and spur activity in this young and rapidly growing research field. Includes cutting-edge methods and protocols Provides step-by-step detail essential for reproducible results Contains key notes and implementation advice from the experts

Insights in Nanobiotechnology 2022/2023: Novel Developments, Current Challenges, and Future Perspectives

Author : Gianni Ciofani
Publisher : Frontiers Media SA
ISBN 13 : 2832540066
Total Pages : 138 pages
Book Rating : 4.8/5 (325 download)

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Book Synopsis Insights in Nanobiotechnology 2022/2023: Novel Developments, Current Challenges, and Future Perspectives by : Gianni Ciofani

Download or read book Insights in Nanobiotechnology 2022/2023: Novel Developments, Current Challenges, and Future Perspectives written by Gianni Ciofani and published by Frontiers Media SA. This book was released on 2023-12-12 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: We are now entering the third decade of the 21st century, and, especially in the last years, the achievements made by scientists have been exceptional, leading to major advancements in the rapidly growing fields of bioengineering and biotechnology. This annual collection, which highlights article submissions from our Editorial Board members, looks to explore new insights, novel developments, current challenges, latest discoveries, recent advances, and future perspectives in the field of Nanobiotechnology. The Research Topic solicits brief, forward-looking contributions that describe the state of the art, outlining recent developments and major accomplishments that have been achieved and that need to occur to move the field forward. Authors are encouraged to identify the greatest challenges in the sub-disciplines, and how to address those challenges. The goal of this special edition Research Topic is to shed light on the progress made in the past decade and on its future challenges to provide a thorough overview of the nanobiotechnology field. This article collection will inspire, inform and provide direction and guidance to researchers. This collection is part of the 'Insights In' series which has been launched in each section of the journal. Other titles in the series include: Insights in Bioprocess Engineering 2022: Novel Developments, Current Challenges, and Future Perspective Insights in Biomaterials 2022: Novel Developments, Current Challenges, and Future Perspectives Insights In Biosafety & Biosecurity 2022: Novel Developments, Current Challenges, and Future Perspectives Insights in Biofabrication 2022: Novel Developments, Current Challenges, and Future Perspectives Insights in Tissue Engineering and Regenerative Medicine 2022 / 2023: Novel Developments, Current Challenges, and Future Perspectives Insights in Synthetic Biology 2022 / 2023: Novel Developments, Current Challenges, and Future Perspectives

Protein Self-Assembly

Author : Jennifer J. McManus
Publisher : Humana
ISBN 13 : 9781493996803
Total Pages : 266 pages
Book Rating : 4.9/5 (968 download)

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Book Synopsis Protein Self-Assembly by : Jennifer J. McManus

Download or read book Protein Self-Assembly written by Jennifer J. McManus and published by Humana. This book was released on 2020-08-08 with total page 266 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores experimental and computational approaches to measuring the most widely studied protein assemblies, including condensed liquid phases, aggregates, and crystals. The chapters in this book are organized into three parts: Part One looks at the techniques used to measure protein-protein interactions and equilibrium protein phases in dilute and concentrated protein solutions; Part Two describes methods to measure kinetics of aggregation and to characterize the assembled state; and Part Three details several different computational approaches that are currently used to help researchers understand protein self-assembly. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Thorough and cutting-edge, Protein Self-Assembly: Methods and Protocols is a valuable resource for researchers who are interested in learning more about this developing field.

Nano-Energetic Materials

Author : Shantanu Bhattacharya
Publisher : Springer
ISBN 13 : 981133269X
Total Pages : 297 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Nano-Energetic Materials by : Shantanu Bhattacharya

Download or read book Nano-Energetic Materials written by Shantanu Bhattacharya and published by Springer. This book was released on 2018-11-09 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents the latest research on the area of nano-energetic materials, their synthesis, fabrication, patterning, application and integration with various MEMS systems and platforms. Keeping in mind the applications for this field in aerospace and defense sectors, the articles in this volume contain contributions by leading researchers in the field, who discuss the current challenges and future perspectives. This volume will be of use to researchers working on various applications of high-energy research.

Structural DNA Nanotechnology

Author : Nadrian C. Seeman
Publisher : Cambridge University Press
ISBN 13 : 0521764483
Total Pages : 269 pages
Book Rating : 4.5/5 (217 download)

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Book Synopsis Structural DNA Nanotechnology by : Nadrian C. Seeman

Download or read book Structural DNA Nanotechnology written by Nadrian C. Seeman and published by Cambridge University Press. This book was released on 2015 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.

Gold Nanostars

Author : Giuseppe Chirico
Publisher : Springer
ISBN 13 : 3319207687
Total Pages : 93 pages
Book Rating : 4.3/5 (192 download)

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Book Synopsis Gold Nanostars by : Giuseppe Chirico

Download or read book Gold Nanostars written by Giuseppe Chirico and published by Springer. This book was released on 2015-08-03 with total page 93 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Brief focuses on the synthesis, functionalization techniques, optical properties and biomedical application of gold nanostars (GNS). Various facilities of gold nanostars synthesis as well as functionalization of GNS with PEG, organic dyes, bioactive compounds are discussed. The authors discuss physical origin of the Localized Surface Plasmon Resonances and the way the nano-environment affects them. The implication of the LSPR of gold nanostars surface enhanced Raman scattering is also discussed. The emphasis has been done on the application of GNS for current and emerge needs of medicine, biology and pharmacy. Moreover, properties of gold nanostars as contrast agents for in vivo imaging and interaction of GNS with cells are also discussed in this Brief.

Issues in Technology Theory, Research, and Application: 2011 Edition

Author :
Publisher : ScholarlyEditions
ISBN 13 : 1464964025
Total Pages : 2926 pages
Book Rating : 4.4/5 (649 download)

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Book Synopsis Issues in Technology Theory, Research, and Application: 2011 Edition by :

Download or read book Issues in Technology Theory, Research, and Application: 2011 Edition written by and published by ScholarlyEditions. This book was released on 2012-01-09 with total page 2926 pages. Available in PDF, EPUB and Kindle. Book excerpt: Issues in Technology Theory, Research, and Application: 2011 Edition is a ScholarlyEditions™ eBook that delivers timely, authoritative, and comprehensive information about Technology Theory, Research, and Application. The editors have built Issues in Technology Theory, Research, and Application: 2011 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Technology Theory, Research, and Application in this eBook to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Issues in Technology Theory, Research, and Application: 2011 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Self-Assembly of Nanostructures and Patchy Nanoparticles

Author : Shafigh Mehraeen
Publisher : BoD – Books on Demand
ISBN 13 : 1789239605
Total Pages : 102 pages
Book Rating : 4.7/5 (892 download)

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Book Synopsis Self-Assembly of Nanostructures and Patchy Nanoparticles by : Shafigh Mehraeen

Download or read book Self-Assembly of Nanostructures and Patchy Nanoparticles written by Shafigh Mehraeen and published by BoD – Books on Demand. This book was released on 2020-11-04 with total page 102 pages. Available in PDF, EPUB and Kindle. Book excerpt: Top-down approaches are currently the main contributor of fabricating microelectronic devices. However, the prohibitive cost of numerous technological steps in these approaches is the main obstacle to further progress. Furthermore, a large number of applications necessitate fabrication of complex and ultra-small devices that cannot be made using these approaches. New approaches based on natural self-assembly of matter need to be developed to allow for fabrication of micro and nanoelectronic devices. Self-assembly of nanostructures is a dynamic field, which explores physics of these structures and new ways to fabricate them. However, the major problem is how to control the properties of the nanostructures resulting from low dimensionality. This book presents recent advances made to address this problem, and fabricate nanostructures using self-assembly.

Polymer Colloids

Author : Rodney Priestley
Publisher : Royal Society of Chemistry
ISBN 13 : 1788014170
Total Pages : 442 pages
Book Rating : 4.7/5 (88 download)

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Book Synopsis Polymer Colloids by : Rodney Priestley

Download or read book Polymer Colloids written by Rodney Priestley and published by Royal Society of Chemistry. This book was released on 2019-12-02 with total page 442 pages. Available in PDF, EPUB and Kindle. Book excerpt: Academic and industrial research around polymer-based colloids is huge, driven both by the development of mature technologies, e.g. latexes for coatings, as well as the advancement of new materials and applications, such as building blocks for 2D/3D structures and medicine. Edited by two world-renowned leaders in polymer science and engineering, this is a fundamental text for the field. Based on a specialised course by the editors, this book provides the reader with an invaluable single source of reference. The first section describes formation, explaining basic properties of emulsions and dispersion polymerization, microfluidic approaches to produce polymer-based colloids and formation via directed self-assembly. The next section details characterisation methodologies from microscopy and small angle scattering, to surface science and simulations. The final chapters close with applications, including Pickering emulsions and molecular engineering for materials development. A comprehensive guide to polymer colloids, with contributions by leaders in their respective areas, this book is a must-have for researchers and practitioners working across polymers, soft matter and chemical and molecular engineering.

Campanian Foraminifera from the Stanford University Campus, California

Author : Joseph John Graham
Publisher :
ISBN 13 :
Total Pages : 278 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Campanian Foraminifera from the Stanford University Campus, California by : Joseph John Graham

Download or read book Campanian Foraminifera from the Stanford University Campus, California written by Joseph John Graham and published by . This book was released on 1963 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Nanotechnology for Microfluidics

Author : Xingyu Jiang
Publisher : John Wiley & Sons
ISBN 13 : 3527345337
Total Pages : 444 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Nanotechnology for Microfluidics by : Xingyu Jiang

Download or read book Nanotechnology for Microfluidics written by Xingyu Jiang and published by John Wiley & Sons. This book was released on 2020-09-08 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book focuses on microfluidics with applications in nanotechnology. The first part summarizes the recent advances and achievements in the field of microfluidic technology, with emphasize on the the influence of nanotechnology. The second part introduces various applications of microfluidics in nanotechnology, such as drug delivery, tissue engineering and biomedical diagnosis.

Mesoscale Chemistry

Author : National Research Council
Publisher : National Academies Press
ISBN 13 : 030937331X
Total Pages : 229 pages
Book Rating : 4.3/5 (93 download)

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Book Synopsis Mesoscale Chemistry by : National Research Council

Download or read book Mesoscale Chemistry written by National Research Council and published by National Academies Press. This book was released on 2015-08-06 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last few decades great strides have been made in chemistry at the nanoscale, where the atomic granularity of matter and the exact positions of individual atoms are key determinants of structure and dynamics. Less attention, however, has been paid to the mesoscale-it is at this scale, in the range extending from large molecules (10 nm) through viruses to eukaryotic cells (10 microns), where interesting ensemble effects and the functionality that is critical to macroscopic phenomenon begins to manifest itself and cannot be described by laws on the scale of atoms and molecules alone. To further explore how knowledge about mesoscale phenomena can impact chemical research and development activities and vice versa, the Chemical Sciences Roundtable of the National Research Council convened a workshop on mesoscale chemistry in November 2014. With a focus on the research on chemical phenomena at the mesoscale, participants examined the opportunities that utilizing those behaviors can have for developing new catalysts, adding new functionality to materials, and increasing our understanding of biological and interfacial systems. The workshop also highlighted some of the challenges for analysis and description of mesoscale structures. This report summarizes the presentations and discussion of the workshop.

Metal Nanoparticles in Microbiology

Author : Mahendra Rai
Publisher : Springer Science & Business Media
ISBN 13 : 3642183123
Total Pages : 306 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Metal Nanoparticles in Microbiology by : Mahendra Rai

Download or read book Metal Nanoparticles in Microbiology written by Mahendra Rai and published by Springer Science & Business Media. This book was released on 2011-04-02 with total page 306 pages. Available in PDF, EPUB and Kindle. Book excerpt: Following an introduction to biogenic metal nanoparticles, this book presents how they can be biosynthesized using bacteria, fungi and yeast, as well as their potential applications in biomedicine. It is shown that the synthesis of nanoparticles using microbes is eco-friendly and results in reproducible metal nanoparticles of well-defined sizes, shapes and structures. This biotechnological approach based on the process of biomineralization exploits the effectiveness and flexibility of biological systems. Chapters include practical protocols for microbial synthesis of nanoparticles and microbial screening methods for isolating a specific nanoparticle producer as well as reviews on process optimization, industrial scale production, biomolecule-nanoparticle interactions, magnetosomes, silver nanoparticles and their numerous applications in medicine, and the application of gold nanoparticles in developing sensitive biosensors.

RNA Nanotechnology

Author : Bin Wang
Publisher : CRC Press
ISBN 13 : 9814411647
Total Pages : 468 pages
Book Rating : 4.8/5 (144 download)

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Book Synopsis RNA Nanotechnology by : Bin Wang

Download or read book RNA Nanotechnology written by Bin Wang and published by CRC Press. This book was released on 2014-04-02 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the past few decades there has been incredible growth in "bionano"-related research, which has been accompanied by numerous publications in this field. Although various compilations address topics related to deoxyribonucleic acid (DNA) and protein, there are few books that focus on determining the structure of ribonucleic acid (RNA) and using RNA as building blocks to construct nanoarchitectures for biomedical and healthcare applications. RNA Nanotechnology is a comprehensive volume that details both the traditional approaches and the latest developments in the field of RNA-related technology. This book targets a wide audience: a broad introduction provides a solid academic background for students, researchers, and scientists who are unfamiliar with the subject, while the in-depth descriptions and discussions are useful for advanced professionals. The book opens with reviews on the basic aspects of RNA biology, computational approaches for predicting RNA structures, and traditional and emerging experimental approaches for probing RNA structures. This section is followed by explorations of the latest research and discoveries in RNA nanotechnology, including the design and construction of RNA-based nanostructures. The final segment of the book includes descriptions and discussions of the potential biological and therapeutic applications of small RNA molecules, such as small/short interfering RNAs (siRNAs), microRNAs (miRNAs), RNA aptamers, and ribozymes.

Computational Chemistry and Molecular Modeling

Author : K. I. Ramachandran
Publisher : Springer Science & Business Media
ISBN 13 : 3540773045
Total Pages : 405 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Computational Chemistry and Molecular Modeling by : K. I. Ramachandran

Download or read book Computational Chemistry and Molecular Modeling written by K. I. Ramachandran and published by Springer Science & Business Media. This book was released on 2008-05-20 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: The gap between introductory level textbooks and highly specialized monographs is filled by this modern textbook. It provides in one comprehensive volume the in-depth theoretical background for molecular modeling and detailed descriptions of the applications in chemistry and related fields like drug design, molecular sciences, biomedical, polymer and materials engineering. Special chapters on basic mathematics and the use of respective software tools are included. Numerous numerical examples, exercises and explanatory illustrations as well as a web site with application tools (http://www.amrita.edu/cen/ccmm) support the students and lecturers.

Flow Cytometry

Author : Alice Longobardi Givan
Publisher : John Wiley & Sons
ISBN 13 : 1118688392
Total Pages : 309 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Flow Cytometry by : Alice Longobardi Givan

Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.