Indirect Detection Techniques In High Performance Liquid Chromatography And Ion Chromatography

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Handbook of Pharmaceutical Analysis by HPLC

Author : Satinder Ahuja
Publisher : Elsevier
ISBN 13 : 0080455182
Total Pages : 679 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Handbook of Pharmaceutical Analysis by HPLC by : Satinder Ahuja

Download or read book Handbook of Pharmaceutical Analysis by HPLC written by Satinder Ahuja and published by Elsevier. This book was released on 2005-02-09 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: High pressure liquid chromatography–frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening). A complete reference guide to HPLC Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling

Selective Sample Handling and Detection in High-Performance Liquid Chromatography

Author : K. Zech
Publisher : Elsevier
ISBN 13 : 0080858430
Total Pages : 407 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Selective Sample Handling and Detection in High-Performance Liquid Chromatography by : K. Zech

Download or read book Selective Sample Handling and Detection in High-Performance Liquid Chromatography written by K. Zech and published by Elsevier. This book was released on 1989-10-30 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is the second of a two-volume project which treats the handling, separation and detection of complex samples as an integrated, interconnected process. On the basis of this philosophy the editors have selected those contributions which demonstrate that optimal sample preparation leads to a simplification of detection or reduced demands on the separation process. Throughout the book emphasis is on chemical principles with minimum discussion of the equipment required - an approach which reflects the editors' view that the limiting factor in the analysis of complex samples is an incomplete knowledge of the underlying chemistry rather than the hardware available. This lack of knowledge becomes more evident as the demands for lower detection limits grow, as solving complex matrix problems requires a greater understanding of the chemical interaction between the substance to be analysed and the stationary phase. Thus, apart from one chapter dealing with chemically modified silicas, the main theme of the book is developed in three chapters on sample preparation and three on detection.The opening chapter outlines concentration and chromatography on chemically modified silicas with complexing properties, and gives examples of the use of these phases with organic and inorganic compounds. Chapter II, the first of the three contributions dealing with sample preparation, addresses such questions as whether the prepared sample is representative of the material to be analysed; how to avoid contamination; which separation procedure should be used to avoid tedious sample preparation. Chapter III describes the processing of whole blood for drug analysis. The determination of cyclosporine and its metabolites (an especially difficult case) demonstrates how comprehensive the optimisation of sample preparation must be to successfully perform the analysis. Several other examples are also given. Chapter IV deals with radio-column liquid chromatography and introduces the other theme of the book, i.e. selective detection methods. The widespread use of radioisotopes requires a high degree of purification during the manufacture of the compounds, as well as highly accurate detection methods in biological and biochemical studies.Chapter V continues the theme of selective detection with an overview of post-column reaction detection. The use of immobilised enzymes in post-column reactors or `pumpless' reactor systems for on-line reagent generation after the chromatographic separation step is discussed in detail. Various examples of the separation of biological compounds show how the production of electrochemical reagents and photochemical reaction detection have increased the selectivity of the detection, leading to more economical analytical systems. Selective detection employing luminescence detection techniques is outlined in Chapter VI. The use of immobilised fluorophores or the coupling to photochemical reactions leads to highly selective detection systems which can greatly simplify the sample handling. The final chapter reviews the use of continuous separation techniques in flow injection analysis thus revealing the need for a strong interdisciplinary dependence between sample handling and separation in this area.Written by experienced practitioners, this book will be extremely useful to investigators in many areas of application. Each chapter includes sufficient references to the literature to serve as a valuable starting point for more detailed investigation. The strong emphasis on sample handling makes the book unique in many ways and it will be welcomed by environmental scientists as well as those active in the clinical, pharmaceutical and bioanalytical fields.

Ion Chromatography

Author : James S. Fritz
Publisher : John Wiley & Sons
ISBN 13 : 3527613250
Total Pages : 267 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Ion Chromatography by : James S. Fritz

Download or read book Ion Chromatography written by James S. Fritz and published by John Wiley & Sons. This book was released on 2008-07-11 with total page 267 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reflecting the tremendous development of ion chromatography in recent years, the best-selling book by Fritz and Gjerde has now gone into a third edition. This is essentially a new book, describing materials, principles, and methods of ion chromatography in a clear and concise style. The book can be used both as an introduction for the new comer and as a practical guide for method development and applications for the experienced user. It contains handy tables with useful data, e. g. on detection and elution conditions. With this new edition, the scope has been enlarged to include capillary electrophoresis as well as chemical speciation. The readers of this book will profit from the authors' background and experience both in education and industrial application.

Determination of Anions in Natural and Treated Waters

Author : T R Crompton
Publisher : CRC Press
ISBN 13 : 0203303237
Total Pages : 817 pages
Book Rating : 4.2/5 (33 download)

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Book Synopsis Determination of Anions in Natural and Treated Waters by : T R Crompton

Download or read book Determination of Anions in Natural and Treated Waters written by T R Crompton and published by CRC Press. This book was released on 2003-09-02 with total page 817 pages. Available in PDF, EPUB and Kindle. Book excerpt: As environmental controls are lagging behind industrial development, metals are an increasing hazard to humans, animal and plant life. Bioaccumulation of metals through the food chain creates a serious impact on public health yet analytical techniques for detecting the often low concentrations of contaminants are poorly understood. Determination of Anions in Natural and Treated Waters draws together the scattered literature and presents in a systematic fashion the latest available analytical techniques for detecting anions in non-saline and saline natural and treated water. Broad outlines of different methods and their applicability in certain situations are given allowing the chemist to choose appropriate test methods.

Practical High-Performance Liquid Chromatography

Author : Veronika R. Meyer
Publisher : John Wiley & Sons
ISBN 13 : 1118681347
Total Pages : 342 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Practical High-Performance Liquid Chromatography by : Veronika R. Meyer

Download or read book Practical High-Performance Liquid Chromatography written by Veronika R. Meyer and published by John Wiley & Sons. This book was released on 2013-03-25 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Jump into the HPLC adventure! Three decades on from publication of the 1st German edition of Veronika Meyer's book on HPLC, this classic text remains one of the few titles available on general HPLC aimed at practitioners. New sections on the following topics have been included in this fifth edition: Comparison of HPLC with capillary electrophoresis How to obtain peak capacity van Deemter curves and other coherences Hydrophilic interaction chromatography Method transfer Comprehensive two-dimensional HPLC Fast separations at 1000 bar HPLC with superheated water In addition, two chapters on the instrument test and troubleshooting in the appendix have been updated and expanded by Bruno E. Lendi, and many details have been improved and numerous references added. A completely new chapter is presented on quality assurance covering: Is it worth the effort? Verification with a second method Method validation Standard operating procedures Measurement uncertainty Qualifications, instrument test, and system suitability test The quest for quality Reviews of earlier editions "That this text is written by an expert in both the practice and teaching of HPLC is evident from the first paragraph....not only an enjoyable, fascinating and easy read, but a truly excellent text that has and will serve many teachers, students and practitioners very well." —The Analyst “...provides essential information on HPLC for LC practitioners in academia, industry, government, and research laboratories...a valuable introduction." - American Journal of Therapeutics

Ion Chromatography

Author : Hamish Small
Publisher : Springer Science & Business Media
ISBN 13 : 0306432900
Total Pages : 292 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis Ion Chromatography by : Hamish Small

Download or read book Ion Chromatography written by Hamish Small and published by Springer Science & Business Media. This book was released on 1989-11-30 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bewitched is an odd word with which to begin a chemical textbook. Yet that is a fair description of how I reacted on first leaming of ion exchange and imagining what might be done with it. That initial fascination has not left me these many years later, and it has provided much ofthe motivation for writing this book. The perceived need for a text on the fundamentals of ion chromatography provided the rest. Many readers will have a general idea of what ion chromatography is and what it does. Briefly, for those who do not, it is an umbrella term for a variety of chromatographie methods for the rapid and sensitive analysis of mixtures of ionic species. It has become highly developed in the last decade, and while it is now routinely used for the determination of organic as weH as inorganic ions, its initial impact was greatest in the area of inorganic analysis. In the past the determination of inorganic ions, particularly anions, meant laborious, time-con suming, and often not very sensitive "wet chemieal" methods. In the last ten years that has changed radically as ion chromatography has supplanted these older methods.

High Performance Liquid Chromatography & Capillary Electrophoresis

Author : Andrea Weston
Publisher : Elsevier
ISBN 13 : 0080534163
Total Pages : 295 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis High Performance Liquid Chromatography & Capillary Electrophoresis by : Andrea Weston

Download or read book High Performance Liquid Chromatography & Capillary Electrophoresis written by Andrea Weston and published by Elsevier. This book was released on 1997-07-21 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: HPLC and CE: Principles and Practice presents the latest information on the most powerful separation techniques available: high-performance liquid chromatography (HPLC) and capillary electrophoresis (CE). Fundamental theory, instrumentation, modes of operation, and optimization of separations are presented in a concise, non-technical style to help the user in choosing the appropriate technique quickly and accurately. Well- illustrated and containing convenientend-of-chapter summaries of the major concepts, the book provides in-depth coverage of trouble-shooting, improvement of resolution, data manipulation, selectivity, and sensitivity.Graduate students, technicians, and researchers who must use separations with little or no background in analytical chemistry can overcome separation anxiety and get started in obtaining the best possible separations in minimal time. The book will alsobe useful to analytical chemists who need a better understanding of theory and processes. - Fully up-to-date information on both HPLC and CE includes troubleshooting and comparisons of the two techniques - Applicable to a wide variety of separation problems - Covers basic concepts governing any separation as well as instrumentation and how to use it - Helps the user to obtain optimal resolution in minimal time - Contains information on special procedures such as chiral separations, affinity chromatography, and sample preparation - Includes information on upcoming trends such as miniaturization - Major concepts in each chapter are organized to allow access to information easily and quickly - Contains practical bibliography for accessing the literature

High Performance Liquid Chromatography

Author : W.J. Lough
Publisher : CRC Press
ISBN 13 : 9780751400762
Total Pages : 292 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis High Performance Liquid Chromatography by : W.J. Lough

Download or read book High Performance Liquid Chromatography written by W.J. Lough and published by CRC Press. This book was released on 1995-09-30 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Ion Chromatography Applications

Author : Robert E. Smith
Publisher : CRC Press
ISBN 13 : 9780849349676
Total Pages : 216 pages
Book Rating : 4.3/5 (496 download)

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Book Synopsis Ion Chromatography Applications by : Robert E. Smith

Download or read book Ion Chromatography Applications written by Robert E. Smith and published by CRC Press. This book was released on 1987-12-31 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: Describes recent advances in ion chromatography and demonstrates how it is used to solve scientific and industrial problems. The basic principles of ion chromatography are explained, including gradient elution of ions and micromembrane suppressors. The various anion and cation exchange columns together with various detection methods and applications of ion chromatography in the environmental and life sciences and industry are reviewed. Over 100 chromatograms which illustrate parameters needed to perform analysis and data on gradient and mobile phase ion chromatography are included.

Ion-Pair Chromatography and Related Techniques

Author : Teresa Cecchi
Publisher : CRC Press
ISBN 13 : 1439800979
Total Pages : 218 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Ion-Pair Chromatography and Related Techniques by : Teresa Cecchi

Download or read book Ion-Pair Chromatography and Related Techniques written by Teresa Cecchi and published by CRC Press. This book was released on 2009-10-15 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion-Pair Chromatography (IPC) is a rapidly evolving method for difficult analyses of organic and inorganic ions and ionogenic, neutral, and zwitterionic compounds. The possibilities for this technology continue to grow as novel ion-pair reagents and strategies are introduced at an accelerated level. Compensating for a dearth in the literature, Ion-

Ion-Exchange Chromatography and Related Techniques

Author : Pavel Nesterenko
Publisher : Elsevier
ISBN 13 : 0443153701
Total Pages : 646 pages
Book Rating : 4.4/5 (431 download)

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Book Synopsis Ion-Exchange Chromatography and Related Techniques by : Pavel Nesterenko

Download or read book Ion-Exchange Chromatography and Related Techniques written by Pavel Nesterenko and published by Elsevier. This book was released on 2023-11-15 with total page 646 pages. Available in PDF, EPUB and Kindle. Book excerpt: Ion-Exchange Chromatography and Related Techniques defines the current state-of-the-art in ion-exchange chromatography and related techniques and their implementation in laboratory and industrial practice. This book provides a compact source of information to facilitate the transfer of knowledge and experience acquired by separation science specialists to colleagues from diverse backgrounds who need to acquire fundamental and practical information to facilitate progress in research and management functions reliant on information acquired by separation. Individual chapters written by recognized experts lending credibility to the work will allow this book to serve as a high value reference source of current information for analytical and biopharmaceutical chemists. - Includes individual chapters written by recognized authoritative and visionary experts in the field to provide an overview and focused treatment of a single topic - Presents comprehensive coverage of ion-exchange techniques from theory, to methods, to selected applications for ions and biopolymers - Provides Tables and diagrams with commonly used data to facilitate practical work, comparison of results and decision-making

A Practical Guide to HPLC Detection

Author : Donald Parriott
Publisher : Academic Press
ISBN 13 : 0323140610
Total Pages : 304 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis A Practical Guide to HPLC Detection by : Donald Parriott

Download or read book A Practical Guide to HPLC Detection written by Donald Parriott and published by Academic Press. This book was released on 2012-12-02 with total page 304 pages. Available in PDF, EPUB and Kindle. Book excerpt: This guide for the practicing chromatographer who wants a ready source of information on HPLC detection explores and compares existing detection systems and detectors, outlines the common problems associated with a given detector, and offers proven approaches to avoiding such problems. - Addresses the practical aspects of HPLC detection, including: basic theory, when a particular type of detector can be used, how detectors from various manufacturers differ, common problems of detectors and ways to avoid them - Presents an overview of today's most common techniques - Discusses the advantages and disadvantages of HPLC, dispelling common misconceptions

Instrumental Analytical Chemistry

Author : James W. Robinson
Publisher : CRC Press
ISBN 13 : 1315301148
Total Pages : 920 pages
Book Rating : 4.3/5 (153 download)

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Book Synopsis Instrumental Analytical Chemistry by : James W. Robinson

Download or read book Instrumental Analytical Chemistry written by James W. Robinson and published by CRC Press. This book was released on 2021-06-29 with total page 920 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical chemistry today is almost entirely instrumental analytical chemistry and it is performed by many scientists and engineers who are not chemists. Analytical instrumentation is crucial to research in molecular biology, medicine, geology, food science, materials science, and many other fields. With the growing sophistication of laboratory equipment, there is a danger that analytical instruments can be regarded as "black boxes" by those using them. The well-known phrase "garbage in, garbage out" holds true for analytical instrumentation as well as computers. This book serves to provide users of analytical instrumentation with an understanding of their instruments. This book is written to teach undergraduate students and those working in chemical fields outside analytical chemistry how contemporary analytical instrumentation works, as well as its uses and limitations. Mathematics is kept to a minimum. No background in calculus, physics, or physical chemistry is required. The major fields of modern instrumentation are covered, including applications of each type of instrumental technique. Each chapter includes: A discussion of the fundamental principles underlying each technique Detailed descriptions of the instrumentation. An extensive and up to date bibliography End of chapter problems Suggested experiments appropriate to the technique where relevant This text uniquely combines instrumental analysis with organic spectral interpretation (IR, NMR, and MS). It provides detailed coverage of sampling, sample handling, sample storage, and sample preparation. In addition, the authors have included many instrument manufacturers’ websites, which contain extensive resources.

Liquid Chromatography

Author : Salvatore Fanali
Publisher : Elsevier
ISBN 13 : 0128093455
Total Pages : 810 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Liquid Chromatography by : Salvatore Fanali

Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-22 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

High Performance Liquid Chromatography

Author : Mary T. Gilbert
Publisher : Elsevier
ISBN 13 : 1483281299
Total Pages : 397 pages
Book Rating : 4.4/5 (832 download)

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Book Synopsis High Performance Liquid Chromatography by : Mary T. Gilbert

Download or read book High Performance Liquid Chromatography written by Mary T. Gilbert and published by Elsevier. This book was released on 2013-10-22 with total page 397 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Performance Liquid Chromatography focuses on the developments, operating techniques, practices, equipment, and packing materials involved in High Performance Liquid Chromatography (HPLC). The book first offers information on basic chromatographic theory, equipment, and the column. Topics include resolution, efficiency, pumps and gradient systems, connectors, detectors, injectors, column packing and testing, packing materials, and coupling of columns. The text also ponders on sample treatment and separation methods, as well as trace analysis, reversed phase chromatography, and selection/optimization conditions. The publication examines adjustment of selectivity by the use of eluent additives and preparative liquid chromatography. Discussions focus on chromatography on dynamically modified oxide gels, metal complexation, crown ethers, ion pair chromatography, materials for preparative chromatography, and separation strategy. The text also reviews the trends in the practice of HPLC and chiral chromatography. The book is a dependable reference for readers interested in High Performance Liquid Chromatography.

Bioinstrumentation and Biosensors

Author : Donald L. Wise
Publisher : CRC Press
ISBN 13 : 9780824783372
Total Pages : 832 pages
Book Rating : 4.7/5 (833 download)

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Book Synopsis Bioinstrumentation and Biosensors by : Donald L. Wise

Download or read book Bioinstrumentation and Biosensors written by Donald L. Wise and published by CRC Press. This book was released on 1991-01-31 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: This reference text consists of contributed chapters by specialists directly carrying out research and development in this emerging field which joins advanced microelectronics with modern biotechnology. Chapters present novel biotechnology-based microelectronic instruments, such as those used for de

Selectivity and Detectability Optimizations in HPLC

Author : Satinder Ahuja
Publisher : John Wiley & Sons
ISBN 13 : 9780471626459
Total Pages : 642 pages
Book Rating : 4.6/5 (264 download)

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Book Synopsis Selectivity and Detectability Optimizations in HPLC by : Satinder Ahuja

Download or read book Selectivity and Detectability Optimizations in HPLC written by Satinder Ahuja and published by John Wiley & Sons. This book was released on 1989-06-06 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: High Performance Liquid Chromatography Edited by Phyllis Brown and Richard Hartwick This contributed volume is designed to consolidate the basic theories of chromatography along with the more exciting developments in the field. This monograph addresses some questions that concern researchers in separation science, including: what is the current state of the art in liquid chromatography; has the development of liquid chromatography plateaued; if so, what new methods will take its place or complement it; and if not, where will the new frontiers be and what direction will liquid chromatography take? 1989 (0 471-84506-X) 688 pp. Quantitative Structure-Chromatographic Retention Relationships R. Kaliszan Written by a pioneer in the field, this book extends and updates research on quantitative structure retention relationships by consolidating and critically reviewing the extensive literature on the subject, while also providing the basic theoretical and practical information required in all investigations involving chromatography, analytical chemistry, biochemistry, and pharmaceutical research. Among the topics covered are the nature of chromatographic interactions, molecular interpretation of distribution processes in chromatography, topological indices as retention descriptors, and multiparameter structure-chromatographic retention relationships. 1987 (0 471-85983-4) 303 pp. Detectors for Liquid Chromatography Edited by Edward S. Yeung With its singular coverage of this fast-growing field, Detectors for Liquid Chromatography presents the state of the art in this subject area. It offers a comprehensive examination of the basic principles behind the detector response, instrumentation, and selected applications for comparison and evaluation of potential. Specifically, topics given in-depth coverage include polarimetric, indirect absorbance, refractive index detectors, absorption detectors for HPLC, FTIR and fluorometric detection, detection based on electrical and electromechanical measurements, and mass spectroscopy as an on-line detector for HPLC. 1986 (0 471-82169-1) 366 pp.