In Situ Hybridization Histochemistry

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In Situ Hybridization Histochemistry

Author : Marie-Francoise Chesselet
Publisher : CRC Press
ISBN 13 : 9780849369124
Total Pages : 232 pages
Book Rating : 4.3/5 (691 download)

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Book Synopsis In Situ Hybridization Histochemistry by : Marie-Francoise Chesselet

Download or read book In Situ Hybridization Histochemistry written by Marie-Francoise Chesselet and published by CRC Press. This book was released on 1990-07-03 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this fascinating new book is to review the diversity of methods available to apply in situ hybridization histochemistry (ISHH) to a variety of experimental questions. This work includes topics such as synthesis and use of nick-translated DNA probes for ISHH, synthesis and use of oligomeric DNA probes for ISHH, and synthesis and use of RNA probes for ISHH. These interesting chapters describe the preparation of different radiolabeled probes for ISHH. They also discuss their respective advantages and limitations, and describe current results based on the use of these various probes. Sections of the text highlight low and high resolution autoradiography for ISHH, the use of biotin-labeled probes for ISHH, as well as the use of ISHH in combination with established anatomical techniques. In Situ Hybridization Histochemistry answers all of your questions regarding the quantification of ISHH. It also provides a practical description of typical protocols, both from molecular biology and histology. Investigators will understand and value this useful, powerful tool-whatever their backgrounds might be.

In Situ Hybridization: Medical Applications

Author : G.R. Coulton
Publisher : Springer Science & Business Media
ISBN 13 : 9401129843
Total Pages : 126 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis In Situ Hybridization: Medical Applications by : G.R. Coulton

Download or read book In Situ Hybridization: Medical Applications written by G.R. Coulton and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 126 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization has developed as a means of localizing specific DNA and RNA sequences within tissues. The great strength of this approach is the ability to relate the distribution of specific nucleic acids with cell structures and the protein products of the target gene by means of immunohistochemistry. Complementary DNA, RNA or oligonucleotide probes, suitably labelled, are hybridized to specific DNA or RNA targets within tissues. The spatial information thus obtained has contributed greatly to our understanding of such diverse areas of research as gene mapping, viral infection, cytogenetics, protein synthesis, prenatal diagnosis and tissue grafting. This book is not intended as another recipe book, although it does describe theoretical and practical aspects of the technology. Rather, the authors critically describe the contribution made by in situ hybridization to specific areas of medical research.

Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences

Author : Julian E. Beesley
Publisher : Springer Science & Business Media
ISBN 13 : 146120139X
Total Pages : 277 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences by : Julian E. Beesley

Download or read book Immunocytochemistry and In Situ Hybridization in the Biomedical Sciences written by Julian E. Beesley and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunocytochemistry and in situ hybridization are widely used biomedical sciences. They are essential in medical diagnosis and in cell biology research. Affinity labeling is the central goal of the experimental strategy involving a series of techniques in a logical order; from the effects of specimen fixation, through specimen preparation to expose the antigen, to optimizing immunolabeling, to assessing the result and finally to safety considerations. Numerous examples of these techniques in biomedical sciences are included, as well as experimental assays and practical tips. This survey of methods will serve as an invaluable reference source in any laboratory setting (academic, industrial or clinical) involved in research in almost every branch of biology or medicine, as well as in pharmaceutical, biotechnological and clinical applications.

In Situ Hybridization in Neurobiology

Author : James H. Eberwine
Publisher :
ISBN 13 : 9780195075076
Total Pages : 230 pages
Book Rating : 4.0/5 (75 download)

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Book Synopsis In Situ Hybridization in Neurobiology by : James H. Eberwine

Download or read book In Situ Hybridization in Neurobiology written by James H. Eberwine and published by . This book was released on 1994 with total page 230 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization has become an important and widely used research tool for neurobiologists since the authors' first book on the subject, In Situ Hybridization: Applications to Neurobiology (Oxford, 1987). This new volume examines the methodological advances that have been made in in situhybridization techniques and their impact on the study of the nervous system. These include methods for improving sensitivity, ways of examining multiple mRNAs within the same section, ways of examining both proteins and mRNAs within the same section, and methods for using in situ hybridizationtechnology to examine areas of cellular biological significance, such as translational control. The development of alternative labels to radioactivity for the detection of hybridized probes, and the combined use of in situ hybridization and immunocytochemistry is also covered in this book. Itdiscusses the quantification of in situ hybridization signal and presents an overview of information generated by using in situ hybridization in diagnostic medicine. This volume will be essential to researchers in the neurosciences as well as those in other disciplines.

Molecular Histochemical Techniques

Author : Takehiko Koji
Publisher : Springer Science & Business Media
ISBN 13 : 4431679154
Total Pages : 270 pages
Book Rating : 4.4/5 (316 download)

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Book Synopsis Molecular Histochemical Techniques by : Takehiko Koji

Download or read book Molecular Histochemical Techniques written by Takehiko Koji and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detailed protocols for analyzing DNA strand breaks, specific RNA/DNA expressions, and DNA binding proteins such as transcription factors at the individual cell level. It describes in situ nick translation and TUNEL in detail, along with radioactive and non-radioactive methods for in situ hybridization. Of special significance is the description of Southwestern histochemistry, which makes it possible to analyze the expression of transcription regulatory factors in individual cells. This volume provides an overview of the current status of molecular histochemistry along with practical how-to-do-it details. The methodology is set out in easy-to-follow language by leading scientists working in the field today. Both a rich source of the latest information and a practical lab manual.

In Situ Hybridization

Author : David G. Wilkinson
Publisher : OUP Oxford
ISBN 13 : 0191565709
Total Pages : 243 pages
Book Rating : 4.1/5 (915 download)

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Book Synopsis In Situ Hybridization by : David G. Wilkinson

Download or read book In Situ Hybridization written by David G. Wilkinson and published by OUP Oxford. This book was released on 1998-11-26 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: In situ hybridization is used to reveal the location of specific nucleic acids sequences on chromosomes or in tissues. Visualization of the location of genes on chromosomes or of specific mRNAs or viruses in tissues is crucial for understanding the organization, regulation, and function of genes. It is a therefore a core technique in all areas of biomedical research. In Situ Hybridization: A Practical Approach 2/e is the second edition of one of the most successful Practical Approach books, published in 1992. Since the first edition was published, a number of important technical advances have been made. The new edition has been thoroughly updated to contain protocols detailing the major techniques of in situ hybridization currently in use: in situ hybridization to mRNA with oligonucleotide and RNA probes (radiolabelled and hapten labelled); analysis using light and electron microscopes; whole mount in situ hybridization; double detection of RNAs, and RNA plus protein; and fluorescent in situ hybridization to detect chromosomal sequences. The protocols are complemented by advice on strategies for successful results, descriptions of the theoretical basis of in situ hybridization and important new developments in gene expression databases. The procedures described are widely applicable to many systems. The use of in situ hybridization in PCR is covered in a separate volume: Herrington and O'Leary (Eds) PCR 3 - PCR in situ hybridization: A Practical Approach (OUP, 1997). All the authors have extensive practical experience of establishing reliable techniques of in situ hybridization. This book will be useful to all researchers at all levels who use in situ hybridization.

In Situ Hybridization Techniques for the Brain

Author : Z. Henderson
Publisher :
ISBN 13 :
Total Pages : 192 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis In Situ Hybridization Techniques for the Brain by : Z. Henderson

Download or read book In Situ Hybridization Techniques for the Brain written by Z. Henderson and published by . This book was released on 1996-12-11 with total page 192 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by molecular biologists and neurobiologists, this volume in the Handbook Series provides basic step-by-step protocols in addition to specialist techniques to support those practising in situ hybridization, a powerful histochemical technique which is now widely used in neurobiology. The first part of the manual concentrates on basic procedures, eg, which probe and label to select, equipment, relevant molecular biological techniques, hazards, probe preparation and labelling, tissue preparation, and conditions for hybridization. Specialist procedures including quantitative analysis of data, reverse transcription PCR, working with human tissue, and double label methods are presented in subsequent sections. In Situ Hybridization Techniques for the Brain is, therefore, an essential reference source for all those with research interests in neuroanatomy, histopathology and/or neurohistochemistry, and will support users in both the preparation and successful completion of the procedures in their own laboratory.

In Situ Hybridization in Brain

Author : George R. Uhl
Publisher : Springer
ISBN 13 :
Total Pages : 330 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis In Situ Hybridization in Brain by : George R. Uhl

Download or read book In Situ Hybridization in Brain written by George R. Uhl and published by Springer. This book was released on 1986-11 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: The explosion of interest in specific molecules important for brain function and dysfunction has drawn individuals from diverse backgrounds toward the use of in situ hybridization techniques. Study of the brain demands the anatomic precision and biochemical specificity that this approach can potentially bring. Workers with backgrounds in peptide neuroanatomy, neuropharmacology, molecular biology, neurovirology, neuropathology, and neurophysiology have joined together in this volume to discuss their initial experiences in applying ill situ hybridization techniques to the study of the brain. The work, although still in an early phase of development, is worthy of initial summary and dissemination. In the area of neuropeptide gene expression alone, investigators represented here describe studies of vasopressin, opiate peptides, oxytocin, vasoactive intestinal peptide, cholecystokinin, and somatostatin. Other contributions provide insight into applications of the technique to studies of the expression of genes for neurotransmitter synthesizing enzymes, viral-encoded genes, trophic factor genes, and the genes selected on the basis of their special roles in the brain. The authors provide an important series of technical perspectives, and describe specific experimental protocols. This volume should be of interest to individuals seeking an introduction to these methods, as well to those desiring an up to date precis of work in this burgeoning area. Dr. Uhl, with the sponsorship of the Howard Hughes Medical Institute, has done a superb job of assembling the leaders in this area, and in organizing the presen ta tion of their perspecti ves herein. Joseph B. Martin, M.D., Ph.D.

Histochemistry

Author : Jinsong Zhou
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110531399
Total Pages : 244 pages
Book Rating : 4.1/5 (15 download)

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Book Synopsis Histochemistry by : Jinsong Zhou

Download or read book Histochemistry written by Jinsong Zhou and published by Walter de Gruyter GmbH & Co KG. This book was released on 2017-08-21 with total page 244 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book systematically illustrates theories and technologies in Histochemistry, including different kinds of enzymes, immunohistochemistry, polymerase chain reaction, related electron microscopic cytochemical techniques as well as the quantitative assay metrology. Abundant experiments as well as vivid images are demonstrated, making the book an essential reference for both graduate students and researchers in biochemistry.

Microquantitative Methods in Enzyme Histochemistry. In Situ Hybridization

Author :
Publisher :
ISBN 13 :
Total Pages : 246 pages
Book Rating : 4.:/5 (489 download)

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Book Synopsis Microquantitative Methods in Enzyme Histochemistry. In Situ Hybridization by :

Download or read book Microquantitative Methods in Enzyme Histochemistry. In Situ Hybridization written by and published by . This book was released on 1989 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt:

In Situ Hybridization Protocols

Author : Ian A. Darby
Publisher : Springer Science & Business Media
ISBN 13 : 1597450073
Total Pages : 272 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis In Situ Hybridization Protocols by : Ian A. Darby

Download or read book In Situ Hybridization Protocols written by Ian A. Darby and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 272 pages. Available in PDF, EPUB and Kindle. Book excerpt: The technique of in situ hybridization, in its various forms, has been used routinely in many laboratories for a number of years. In the post-genome era, gene arrays and proteomics have allowed us to identify hitherto unknown unrecognized pathways and mechanisms. However, rather than diminish the importance of in situ hybridization, the now widespread use of screening te- nologies has increased the need to temporally and spatially localize the dist- bution of mRNA expression. Our intention, in In Situ Hybridization Protocols is to provide ample inf- mation for novices planning to set up the in situ hybridization technique and use it in their laboratory for the first time, as well as giving updates of recent developments for those laboratories where in situ hybridization techniques are already in use. Despite its widespread significance, in situ hybridization has retained a re- tation as one of the more difficult and capricious molecular biological te- niques. This may in part be because of the hybrid nature of the technique, which often requires a mixture of molecular biological and histological skills. The two techniques are usually taught and acquired in different streams of biolo- cal science. The step-by-step and detailed protocols provided in In Situ Hybridization Protocols by researchers active in the field should make it p- sible for both the molecular biologist with little experience of histology and the histologist with little experience of molecular biology to use the technique s- cessfully in their laboratories.

Histological & Histochemical Methods

Author : John Alan Kiernan
Publisher : Pergamon
ISBN 13 :
Total Pages : 454 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Histological & Histochemical Methods by : John Alan Kiernan

Download or read book Histological & Histochemical Methods written by John Alan Kiernan and published by Pergamon. This book was released on 1990 with total page 454 pages. Available in PDF, EPUB and Kindle. Book excerpt: Revised and updated edition (1st was 1981) of a textbook on chemical and physical principles of fixation, staining and histochemistry. For students i all biological subjects using histological techniques, as well as researcher and medical laboratory technologists. Annotation copyright Book News, Inc

In Situ Molecular Pathology and Co-Expression Analyses

Author : Gerard J. Nuovo
Publisher : Academic Press
ISBN 13 : 0124160018
Total Pages : 298 pages
Book Rating : 4.1/5 (241 download)

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Book Synopsis In Situ Molecular Pathology and Co-Expression Analyses by : Gerard J. Nuovo

Download or read book In Situ Molecular Pathology and Co-Expression Analyses written by Gerard J. Nuovo and published by Academic Press. This book was released on 2013-02-07 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Situ Molecular Pathology and Co-Expression Analyses explains, in easy-to-understand language, simplified ways of understanding and performing in situ hybridization and immunohistochemistry tests. The book also focuses on straightforward protocols used to simultaneously detect two or more proteins/nucleic acids within intact tissue by doing co-expression analyses. The fields of in situ hybridization and immunohistochemistry have expanded rapidly due to the use of computer-based analysis. To get the most out of these automated platforms, researchers and diagnostic biomedical investigators must have a solid understanding of the basics of in situ-based tests, protocols, and regimens for troubleshooting. Practicing molecular pathologists, clinical chemists, and toxicologists, as well as clinicians and researchers in training, will benefit from this book's clear presentation of protocols and theoretical framework. Includes over 200 easy-to-follow experimental protocols Features chapter-ending summaries of "Key Points to Remember" to bring beginners up to speed with any seasoned veteran in the field Offers two chapters written by industry leaders in the fields of in situ hybridization, immunohistochemistry, and computer software for co-expression analyses

Molecular Histochemical Techniques

Author : Takehiko Koji
Publisher :
ISBN 13 : 9784431679165
Total Pages : 276 pages
Book Rating : 4.6/5 (791 download)

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Book Synopsis Molecular Histochemical Techniques by : Takehiko Koji

Download or read book Molecular Histochemical Techniques written by Takehiko Koji and published by . This book was released on 2000-04-17 with total page 276 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microquantitative Methods in Enzyme Histochemistry

Author :
Publisher :
ISBN 13 : 9783334002865
Total Pages : pages
Book Rating : 4.0/5 (28 download)

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Book Synopsis Microquantitative Methods in Enzyme Histochemistry by :

Download or read book Microquantitative Methods in Enzyme Histochemistry written by and published by . This book was released on 1989 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of Practical Immunohistochemistry

Author : Fan Lin
Publisher : Springer
ISBN 13 : 1493915789
Total Pages : 756 pages
Book Rating : 4.4/5 (939 download)

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Book Synopsis Handbook of Practical Immunohistochemistry by : Fan Lin

Download or read book Handbook of Practical Immunohistochemistry written by Fan Lin and published by Springer. This book was released on 2015-02-21 with total page 756 pages. Available in PDF, EPUB and Kindle. Book excerpt: In a conceptually current, quick-reference, Question & Answer format, the second edition of Handbook of Practical Immunohistochemistry: Frequently Asked Questions continues to provide a comprehensive and yet concise state-of-the-art overview of the major issues specific to the field of immunohistochemistry. With links to the authors Immunohistochemical Laboratory website, this volume creates a current and up-to-date information system on immunohistochemistry. This includes access to tissue microarrays (TMA) of over 10,000 tumors and normal tissue to validate common diagnostic panels and provide the best reproducible data for diagnostic purposes. Fully revised and updated from the first edition, the new features of the second edition include over 200 additional questions or revised questions with an IHC panel to answer each question; over 250 new color photos and illustrations; over 20 new useful biomarkers; hundreds of new references; several new chapters to cover phosphoproteins, rabbit monoclonal antibodies, multiplex IHC stains, overview of predictive biomarkers, and integration of IHC into molecular pathology; many new coauthors who are international experts in a related field; many updated IHC panels using Geisinger IHC data collected from over 10,000 tumors and normal tissues; and updated appendices containing detailed antibody information for both manual and automated staining procedures. Comprehensive yet practical and concise, the Handbook of Practical Immunohistochemistry: Frequently Asked Questions, Second Edition will be of great value for surgical pathologists, pathology residents and fellows, cytopathologists, and cytotechnologists.

In Situ Hybridization

Author : Julia M. Polak
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 264 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis In Situ Hybridization by : Julia M. Polak

Download or read book In Situ Hybridization written by Julia M. Polak and published by Oxford University Press, USA. This book was released on 1990 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular biology and genetic engineering techniques have revolutionized our concepts of gene activity in cellular function. In situ hybridization has contributed to this revolution. It is the only technique which gives precise data on the content and distribution of DNA and mRNA in intactcells and tissues. It is now an invaluable test in understanding the pathophysiology of cellular functions. This book covers all aspects of in situ hybridization. It describes practical procedures and protocols, the scientific background, areas of application, and the limitations of the technology. The contributing authors are all experts. Each chapter contains up-to-date references, many colourillustrations, the latest advances, and prospects for the future. This practical guide will be invaluable to: molecular, cell, and developmental biologists; cyto- and molecular geneticists; clinicians, especially pathologists, biologists, and microbiologists; and scientists seriously interested inthe pathophysiology of cell function and its regulation.