Immunoelectron Microscopy

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Immunoelectron Microscopy

Author : Steven D. Schwartzbach
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis Immunoelectron Microscopy by : Steven D. Schwartzbach

Download or read book Immunoelectron Microscopy written by Steven D. Schwartzbach and published by Methods in Molecular Biology. This book was released on 2010-07-20 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships.

Electron Microscopy Methods and Protocols

Author : M. A. Nasser Hajibagheri
Publisher : Springer Science & Business Media
ISBN 13 : 1592592015
Total Pages : 292 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri

Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Mitochondria

Author : Dario Leister
Publisher : Springer Science & Business Media
ISBN 13 : 1588296679
Total Pages : 1195 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Mitochondria by : Dario Leister

Download or read book Mitochondria written by Dario Leister and published by Springer Science & Business Media. This book was released on 2007-06-12 with total page 1195 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mitochondrial Genomics and Proteomics Protocols offers a broad collection of methods for studying the molecular biology, function, and features of mitochondria. In the past decade, mitochondrial research has elucidated the important influence of mitochondrial processes on integral cell processes such as apoptosis and cellular aging. This practical guide presents a wide spectrum of mitochondrial methods, each written by specialists with solid experience and intended for implementation by novice and expert researchers alike. Part I introduces major experimental model systems and discusses their specific advantages and limitations for functional analysis of mitochondria. The concise overview of general properties of mitochondrial systems is supplemented by detailed protocols for cultivation of model organisms. Parts II-VI comprise a robust collection of protocols for studying different molecular aspects of mitochondrial functions including: genetics and microbiology, biochemistry, physiology, dynamics and morphology, and functional genomics. Emphasis is placed on new and emerging topics in mitochondrial study, such as the examination of apoptotic effects, fusion and fission of mitochondria, and proteome and transcriptome analysis.

Practical Electron Microscopy

Author : Elaine Evelyn Hunter
Publisher : Cambridge University Press
ISBN 13 : 9780521385398
Total Pages : 212 pages
Book Rating : 4.3/5 (853 download)

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Book Synopsis Practical Electron Microscopy by : Elaine Evelyn Hunter

Download or read book Practical Electron Microscopy written by Elaine Evelyn Hunter and published by Cambridge University Press. This book was released on 1993-09-24 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: For this new edition, the chapters on photography and the electron microscope have been completely rewritten and two new chapters have been added--on immuno electron microscopy using colloidal gold and on useful specialized techniques.

Biomedical Electron Microscopy

Author : Arvid B. Maunsbach
Publisher : Elsevier
ISBN 13 : 0080528090
Total Pages : 569 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Biomedical Electron Microscopy by : Arvid B. Maunsbach

Download or read book Biomedical Electron Microscopy written by Arvid B. Maunsbach and published by Elsevier. This book was released on 1998-11-03 with total page 569 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. - Authored by the key leaders in the biological electron microscopy field - Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines - Introduces students on how to read and interpret electron micrographs

Fine Structure Immunocytochemistry

Author : Gareth Griffiths
Publisher : Springer Science & Business Media
ISBN 13 : 3642770959
Total Pages : 477 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Fine Structure Immunocytochemistry by : Gareth Griffiths

Download or read book Fine Structure Immunocytochemistry written by Gareth Griffiths and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron microscopy in the biological sciences can be divided into two disciplines. The first, concerned with high resolution detail of particles or periodic structures, is mostly based on sound theoretical principles of physics. The second, by far the larger discipline, is interested in the information obtainable from thin sections. The theoretical back ground to those groups of techniques for preparing and looking at thin sections is often inexact and "loose", for want of a better word. What should be chemistry is often closer to alchemy. This kind of electron microscopy is often enshrined with mystical recipes, handed down from generation to generation. Admittedly, many of the processes involved, such as those required to embed tissue in epoxy resins, involve multiple interconnected steps, which make it difficult to follow the details of anyone of these steps. If all these steps are shrouded in some mystery, however, can one really trust the final image that emerges on the EM screen? When we present the data in some semi quantitative form is there really no better way to do it than to categorize the parameters with ++, +/-, etc? What happens when one labels the sections with antibodies? Does the whole business necess arily need to be more of an "art" than a "science"? Upon reflecting on these problems in 1981, I had the impression that many of the multi-authored textbooks that existed then (and that have appeared since) tended to exacerbate or at least perpetuate this

Autopsy Pathology: A Manual and Atlas

Author : Andrew J Connolly
Publisher : Elsevier Health Sciences
ISBN 13 : 0323287808
Total Pages : 403 pages
Book Rating : 4.3/5 (232 download)

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Book Synopsis Autopsy Pathology: A Manual and Atlas by : Andrew J Connolly

Download or read book Autopsy Pathology: A Manual and Atlas written by Andrew J Connolly and published by Elsevier Health Sciences. This book was released on 2015-09-23 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mainstay for pathology residents, Autopsy Pathology is designed with a uniquely combined manual and atlas format that presents today's most complete coverage of performing, interpreting, and reporting post-mortem examinations. This lasting and useful medical reference book offers a practical, step-by-step approach to discussing not only the basics of the specialty, but the performance of specialized autopsy procedures as well. Material is divided into two sections for ease of use: a manual covering specific autopsy procedures, biosafety, generation of autopsy reports, preparation of death certificates, and other essential subjects; and an atlas, organized by organ system, which captures the appearance of the complete spectrum of autopsy findings. Offers expanded coverage of microscopic anatomy. Includes a chapter on performing special dissection procedures that may not be covered during a typical residency. Examines important techniques, such as autopsy photography and radiology, microscopic examination, supplemental laboratory studies, and other investigative approaches. Addresses the latest legal, social, and ethical issues relating to autopsies, as well as quality improvement and assurance. Presents more than 600 full-color photographs depicting common gross and microscopic autopsy findings for every part of the body. Correlates pathologic findings with their clinical causes to enhance diagnostic accuracy. Improved images in the Atlas section provide greater visual understanding. Additional online features include dissection videos demonstrating autopsy techniques; downloadable, commonly used forms for autopsy reports; and calculators for weights and measures. Expert Consult eBook version included with purchase. This enhanced eBook experience offers access to all of the text, figures, images, videos, forms, calculators, and references from the book on a variety of devices.

Biological Field Emission Scanning Electron Microscopy, 2 Volume Set

Author : Roland A. Fleck
Publisher : John Wiley & Sons
ISBN 13 : 1118654064
Total Pages : 741 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Biological Field Emission Scanning Electron Microscopy, 2 Volume Set by : Roland A. Fleck

Download or read book Biological Field Emission Scanning Electron Microscopy, 2 Volume Set written by Roland A. Fleck and published by John Wiley & Sons. This book was released on 2019-04-29 with total page 741 pages. Available in PDF, EPUB and Kindle. Book excerpt: The go‐to resource for microscopists on biological applications of field emission gun scanning electron microscopy (FEGSEM) The evolution of scanning electron microscopy technologies and capability over the past few years has revolutionized the biological imaging capabilities of the microscope—giving it the capability to examine surface structures of cellular membranes to reveal the organization of individual proteins across a membrane bilayer and the arrangement of cell cytoskeleton at a nm scale. Most notable are their improvements for field emission scanning electron microscopy (FEGSEM), which when combined with cryo-preparation techniques, has provided insight into a wide range of biological questions including the functionality of bacteria and viruses. This full-colour, must-have book for microscopists traces the development of the biological field emission scanning electron microscopy (FEGSEM) and highlights its current value in biological research as well as its future worth. Biological Field Emission Scanning Electron Microscopy highlights the present capability of the technique and informs the wider biological science community of its application in basic biological research. Starting with the theory and history of FEGSEM, the book offers chapters covering: operation (strengths and weakness, sample selection, handling, limitations, and preparation); Commercial developments and principals from the major FEGSEM manufacturers (Thermo Scientific, JEOL, HITACHI, ZEISS, Tescan); technical developments essential to bioFEGSEM; cryobio FEGSEM; cryo-FIB; FEGSEM digital-tomography; array tomography; public health research; mammalian cells and tissues; digital challenges (image collection, storage, and automated data analysis); and more. Examines the creation of the biological field emission gun scanning electron microscopy (FEGSEM) and discusses its benefits to the biological research community and future value Provides insight into the design and development philosophy behind current instrument manufacturers Covers sample handling, applications, and key supporting techniques Focuses on the biological applications of field emission gun scanning electron microscopy (FEGSEM), covering both plant and animal research Presented in full colour An important part of the Wiley-Royal Microscopical Series, Biological Field Emission Scanning Electron Microscopy is an ideal general resource for experienced academic and industrial users of electron microscopy—specifically, those with a need to understand the application, limitations, and strengths of FEGSEM.

Electron Microscopy of Plant Pathogens

Author : Kurt Mendgen
Publisher : Springer Science & Business Media
ISBN 13 : 3642758185
Total Pages : 342 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Electron Microscopy of Plant Pathogens by : Kurt Mendgen

Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.

Peroxisomes

Author : Michael Schrader
Publisher : Springer Nature
ISBN 13 : 1071630482
Total Pages : 507 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Peroxisomes by : Michael Schrader

Download or read book Peroxisomes written by Michael Schrader and published by Springer Nature. This book was released on 2023-03-23 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast, and this progress is reflected in the chapters included in this collection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peroxisomes: Methods and Protocols, Second Edition serves as an ideal guide for researchers working on peroxisome- and organelle-based research questions.

Principles and Techniques of Electron Microscopy

Author : M. A. Hayat
Publisher : John Wiley & Sons
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Principles and Techniques of Electron Microscopy by : M. A. Hayat

Download or read book Principles and Techniques of Electron Microscopy written by M. A. Hayat and published by John Wiley & Sons. This book was released on 1970 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative mapping with the electron microscope; Photographic aspects of electron microscopy; Environmental devices in electron microscopy; Optical diffractometry; The analytical electron microscope, emma-4.

Resin Microscopy and On-Section Immunocytochemistry

Author : Geoffrey R. Newman
Publisher : Springer Science & Business Media
ISBN 13 : 3642569307
Total Pages : 291 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Resin Microscopy and On-Section Immunocytochemistry by : Geoffrey R. Newman

Download or read book Resin Microscopy and On-Section Immunocytochemistry written by Geoffrey R. Newman and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 291 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since antibodies tagged with markers have been developed, immunocytochemistry has become an important method for identifying tissue substances and the localisation of nucleic acid in tissue by in situ hybridisation in molecular biology. This laboratory book covers the embedding of tissue using less sensitive epoxy resin methods to the more sensitive procedures employing the acrylics. The possibilities are discussed and results are presented so that an understanding of the techniques can be acquired.

Basic Methods in Microscopy

Author : David L. Spector
Publisher : CSHL Press
ISBN 13 : 0879697512
Total Pages : 384 pages
Book Rating : 4.8/5 (796 download)

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Book Synopsis Basic Methods in Microscopy by : David L. Spector

Download or read book Basic Methods in Microscopy written by David L. Spector and published by CSHL Press. This book was released on 2006 with total page 384 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual contains selected material from Cells - a Laboratory Manual, as well as two chapters from Live Cell Imaging. It includes sections on microscopy, and on preparing and labelling specimens for microscopy.

Methods in Plant Electron Microscopy and Cytochemistry

Author : William V. Dashek
Publisher : Springer Science & Business Media
ISBN 13 : 1592592325
Total Pages : 297 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Methods in Plant Electron Microscopy and Cytochemistry by : William V. Dashek

Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

Diagnostic Electron Microscopy

Author : John Stirling
Publisher : John Wiley & Sons
ISBN 13 : 1119973996
Total Pages : 492 pages
Book Rating : 4.1/5 (199 download)

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Book Synopsis Diagnostic Electron Microscopy by : John Stirling

Download or read book Diagnostic Electron Microscopy written by John Stirling and published by John Wiley & Sons. This book was released on 2013-01-22 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diagnostic Electron Microscopy Diagnostic Electron Microscopy: A Practical Guide to Interpretation and Technique summarises the current interpretational applications of TEM in diagnostic pathology. This concise and accessible volume provides a working guide to the main, or most useful, applications of the technique including practical topics of concern to laboratory scientists, brief guides to traditional tissue and microbiological preparation techniques, microwave processing, digital imaging and measurement uncertainty. The text features both a screening and interpretational guide for TEM diagnostic applications and current TEM diagnostic tissue preparation methods pertinent to all clinical electron microscope units worldwide. Containing high-quality representative images, this up-to-date text includes detailed information on the most important diagnostic applications of transmission electron microscopy as well as instructions for specific tissues and current basic preparative techniques. The book is relevant to trainee pathologists and practising pathologists who are expected to understand and evaluate/screen tissues by TEM. In addition, technical and scientific staff involved in tissue preparation and diagnostic tissue evaluation/screening by TEM will find this text useful.

Advanced Techniques in Biological Electron Microscopy

Author : J.K. Koehler
Publisher : Springer Science & Business Media
ISBN 13 : 3642654924
Total Pages : 319 pages
Book Rating : 4.6/5 (426 download)

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Book Synopsis Advanced Techniques in Biological Electron Microscopy by : J.K. Koehler

Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Atlas of Human Reproduction

Author : E.S. Hafez
Publisher : Springer Science & Business Media
ISBN 13 : 9401181403
Total Pages : 337 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Atlas of Human Reproduction by : E.S. Hafez

Download or read book Atlas of Human Reproduction written by E.S. Hafez and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: The suggestion of Max Knoll that an electron fascinated by the numerous SEM photographs, the wealth of information and the enthusiasm of the microscope could be developed using a fine scanning researchers covering a variety of disciplines. All aspects beam of electrons on a specimen surface and recording the emitted current as a function of the position of the of the female and male genital tract have been covered, beam was launched in 1935. Since then several culminating in the prizewinning award showing the in investigators and clinicians have used this concept to vitro fertilized human egg. develop techniques now known as scanning electron In clinical diagnostics SEM also proved to be a microscopy (SEM) and scanning transmission electron valuable complementary technique, shedding new light microscopy (STEM). The choice to study the female on oncology, the pathogenesis of tubal disease and the reproductive organs was a logical one because cells and maturation process of the placenta. Future research has tissue samples can be sampled relatively easily; still to be accomplished; e.g. quantification of SEM furthermore, these cells and organs are influenced photographs for meaningful and sound biological, continuously by the cyclic production of hormones. scientific and statistical evaluation in diagnostic This atlas demonstrates the state of the art in 1983. gynecology, obstetrics, andrology and oncology.