Immunoelectron Microscopy

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Immunoelectron Microscopy

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Author : Steven D. Schwartzbach
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 370 pages
Book Rating : 4.F/5 ( download)

Book Synopsis Immunoelectron Microscopy by : Steven D. Schwartzbach

Download or read book Immunoelectron Microscopy written by Steven D. Schwartzbach and published by Methods in Molecular Biology. This book was released on 2010-07-20 with total page 370 pages. Available in PDF, EPUB and Kindle. Book excerpt: Immunoelectron microscopy is a key technique that bridges the information gap between biochemistry, molecular biology, and ultrastructural studies placing macromolecular functions within a cellular context. In Immunoelectron Microscopy: Methods and Protocols, expert researchers combine the tools of the molecular biologist with those of the microscopist. From the molecular biology toolbox, this volume presents methods for antigen production by protein expression in bacterial cells, methods for epitope tagged protein expression in plant and animal cells allowing protein localization in the absence of protein specific antibodies as well as methods for the production of anti-peptide, monoclonal, and polyclonal antibodies. From the microscopy toolbox, sample preparation methods for cells, plant, and animal tissue are presented. Both cryo-methods, which have the advantage of retaining protein antigenicity at the expense of ultrastructural integrity, as well as chemical fixation methods that maintain structural integrity while sacrificing protein antigenicity have been included, with chapters examining various aspects of immunogold labeling. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and essential, Immunoelectron Microscopy: Methods and Protocols seeks to facilitate an increased understanding of structure function relationships.

Immuno-Gold Electron Microscopy in Virus Diagnosis and Research

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Author : Alexander D. Hyatt
Publisher : CRC Press
ISBN 13 : 9780849367595
Total Pages : 476 pages
Book Rating : 4.3/5 (675 download)

Book Synopsis Immuno-Gold Electron Microscopy in Virus Diagnosis and Research by : Alexander D. Hyatt

Download or read book Immuno-Gold Electron Microscopy in Virus Diagnosis and Research written by Alexander D. Hyatt and published by CRC Press. This book was released on 1992-11-10 with total page 476 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a wide variety of immuno-gold techniques for use in virus diagnosis and research. Protocols are presented for state-of-the-art techniques, including in situ hybridization, freeze substitution, and the utilization of ultra-small probes and replicas for use by virologists and electron microscopists identifying and studying viruses, their components, and replication in cells. The procedures are described by eminent scientists and are pertinent to both experienced researchers and newcomers to this field who are interested in the localization of low antigenic mass structures.

Electron Microscopy Methods and Protocols

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Author : M. A. Nasser Hajibagheri
Publisher : Springer Science & Business Media
ISBN 13 : 1592592015
Total Pages : 292 pages
Book Rating : 4.5/5 (925 download)

Book Synopsis Electron Microscopy Methods and Protocols by : M. A. Nasser Hajibagheri

Download or read book Electron Microscopy Methods and Protocols written by M. A. Nasser Hajibagheri and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron Microscopy Methods and Protocols is designed for the established researcher as a manual for extending knowledge of the field. It is also for the newcomer who wishes to move into the field. A wide range of applications for the examination of cells, tissues, biological macromolecules, molecular structures, and their interactions are discussed. We have tried to gather together methods that we consider to be those most generally appli- ble to current research in both cell and molecular biology. Each chapter c- tains a set of related practical protocols with examples provided by experts who have first-hand knowledge of the techniques they describe. The individual chapters are grouped according to similarities in their specimen preparation and methodology. Methods are presented in detail, in a step-by-step fashion, using reproducible protocols the authors have personally checked. During the last decade, the scientific literature describing the use of colloidal gold as an immunocytochemical marker has increased at an ex- nential rate, and this trend is expected to continue. We have included a large number of variations on the immunogold labeling technique. In both the ne- tive staining and cryo chapters, authors emphasize the “immunological app- cations” in order to correlate as fully as possible with the emphasis on immunogold labeling in the other chapters. Electron Microscopy Methods and Protocols commences with the routine preparation of biological material for classical transmission electron microscopy involving tissue fixation, embedding, and sectioning (Chap. 1).

Microinjection and Transgenesis

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Author : Angel Cid-Arregui
Publisher : Springer Science & Business Media
ISBN 13 : 3642803431
Total Pages : 619 pages
Book Rating : 4.6/5 (428 download)

Book Synopsis Microinjection and Transgenesis by : Angel Cid-Arregui

Download or read book Microinjection and Transgenesis written by Angel Cid-Arregui and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 619 pages. Available in PDF, EPUB and Kindle. Book excerpt: The establishment of microinjection protocols about 20 years ago for cultured cells and shortly thereafter for the generation of transgenic mice by microinjection of DNA into fertilized mouse eggs greatly influ enced many fields of biology. Not only have the data generated using these approaches contributed to a large extent to our present under standing of gene regulation and cellular function of higher eukaryotic cells, but current knowledge and future developments in this area will certainly have a great impact on basic and applied research for many years to come. This laboratory manual describes the current state of the art in this research area and focuses primarily on both the experimental strategies with an extensive bibliography and the detailed procedures. A large number of studies are presently being performed and a great variety of new experimental designs are rapidly being developed. The book con tains protocols on injection of somatic cells as well as on injection of embryos, the use of similar equipment being a common feature. In the articles dedicated to somatic cells, full descriptions of the manual and automatic injection systems are given as well as the methods for the analysis of injected cells by video-microscopy, electron microscopy or in situ hybridizations. In addition, comprehensive protocols are given for injection experiments with very different purposes, such as to study sig nal transduction or microtubule dynamics.

Autopsy Pathology: A Manual and Atlas

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Author : Andrew Connolly
Publisher : Elsevier Health Sciences
ISBN 13 : 0323287808
Total Pages : 403 pages
Book Rating : 4.3/5 (232 download)

Book Synopsis Autopsy Pathology: A Manual and Atlas by : Andrew Connolly

Download or read book Autopsy Pathology: A Manual and Atlas written by Andrew Connolly and published by Elsevier Health Sciences. This book was released on 2015-09-23 with total page 403 pages. Available in PDF, EPUB and Kindle. Book excerpt: A mainstay for pathology residents, Autopsy Pathology is designed with a uniquely combined manual and atlas format that presents today's most complete coverage of performing, interpreting, and reporting post-mortem examinations. This lasting and useful medical reference book offers a practical, step-by-step approach to discussing not only the basics of the specialty, but the performance of specialized autopsy procedures as well. Material is divided into two sections for ease of use: a manual covering specific autopsy procedures, biosafety, generation of autopsy reports, preparation of death certificates, and other essential subjects; and an atlas, organized by organ system, which captures the appearance of the complete spectrum of autopsy findings. Offers expanded coverage of microscopic anatomy. Includes a chapter on performing special dissection procedures that may not be covered during a typical residency. Examines important techniques, such as autopsy photography and radiology, microscopic examination, supplemental laboratory studies, and other investigative approaches. Addresses the latest legal, social, and ethical issues relating to autopsies, as well as quality improvement and assurance. Presents more than 600 full-color photographs depicting common gross and microscopic autopsy findings for every part of the body. Correlates pathologic findings with their clinical causes to enhance diagnostic accuracy. Improved images in the Atlas section provide greater visual understanding. Additional online features include dissection videos demonstrating autopsy techniques; downloadable, commonly used forms for autopsy reports; and calculators for weights and measures. Expert Consult eBook version included with purchase. This enhanced eBook experience offers access to all of the text, figures, images, videos, forms, calculators, and references from the book on a variety of devices.

Advanced Techniques in Biological Electron Microscopy

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Author : J.K. Koehler
Publisher : Springer Science & Business Media
ISBN 13 : 3642654924
Total Pages : 319 pages
Book Rating : 4.6/5 (426 download)

Book Synopsis Advanced Techniques in Biological Electron Microscopy by : J.K. Koehler

Download or read book Advanced Techniques in Biological Electron Microscopy written by J.K. Koehler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 319 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has seen a remarkable increase in the use of electron microscopy as a researm tool in biology and medicine. Thus, most institu tions of higher learning now boast several electron optical laboratories having various levels of sophistication. Training in the routine use of elec tron optical equipment and interpretation of results is no longer restricted to a few prestigious centers. On the other hand, temniques utilized by researm workers in the ultrastructural domain have become extremely diverse and complex. Although a large number of quite excellent volumes of electron microscopic temnique are now dedicated to the basic elements available whim allow the novice to acquire a reasonable introduction to the field, relatively few books have been devoted to a discussion of more ad vanced temnical aspects of the art. It was with this view that the present volume was conceived as a handy reference for workers already having some background in the field, as an information source for those wishing to shift efforts into more promising temniques, or for use as an advanced course or seminar guide. Subject matter has been mosen particularly on the basis of pertinence to present researm activities in biological electron microscopy and emphasis has been given those areas whim seem destined to greatly expand in useful ness in the near future.

Guide to Yeast Genetics and Molecular and Cell Biology, Part C

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Author : Gerald R. Fink
Publisher : Gulf Professional Publishing
ISBN 13 : 9780121822545
Total Pages : 786 pages
Book Rating : 4.8/5 (225 download)

Book Synopsis Guide to Yeast Genetics and Molecular and Cell Biology, Part C by : Gerald R. Fink

Download or read book Guide to Yeast Genetics and Molecular and Cell Biology, Part C written by Gerald R. Fink and published by Gulf Professional Publishing. This book was released on 2002 with total page 786 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume and its companion, Volume 350, are specifically designed to meet the needs of graduate students and postdoctoral students as well as researchers, by providing all the up-to-date methods necessary to study genes in yeast. Procedures are included that enable newcomers to set up a yeast laboratory and to master basic manipulations. Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines. Specific topics addressed in this book include cytology, biochemistry, cell fractionation, and cell biology.

Peroxisomes

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Author : Michael Schrader
Publisher : Springer Nature
ISBN 13 : 1071630482
Total Pages : 507 pages
Book Rating : 4.0/5 (716 download)

Book Synopsis Peroxisomes by : Michael Schrader

Download or read book Peroxisomes written by Michael Schrader and published by Springer Nature. This book was released on 2023-03-23 with total page 507 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated volume assembles a comprehensive collection of methods, techniques, and strategies to investigate the molecular and cellular biology of peroxisomes in different organisms. Peroxisome research is on the rise, as novel functions and proteins of this dynamic organelle are still being discovered through studies in model systems including humans, mice, flies, plants, fungi, and yeast, and this progress is reflected in the chapters included in this collection. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and up-to-date, Peroxisomes: Methods and Protocols, Second Edition serves as an ideal guide for researchers working on peroxisome- and organelle-based research questions.

Electron Microscopic Immunocytochemistry

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Author : Julia M. Polak
Publisher : Oxford University Press, USA
ISBN 13 :
Total Pages : 292 pages
Book Rating : 4.3/5 (91 download)

Book Synopsis Electron Microscopic Immunocytochemistry by : Julia M. Polak

Download or read book Electron Microscopic Immunocytochemistry written by Julia M. Polak and published by Oxford University Press, USA. This book was released on 1992 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electron microscopic immunocytochemistry is a powerful technique combining the resolution of the electron microscope with the specificity and sensitivity of immunocytochemistry. This book discusses methods designed to make this intricate procedure more accessible, and covers recent developments that have simplified the technique and broadened the range of applications. It presents the principles of EM immunocytochemistry, detailed protocols, and examples of ways in which the technique can be applied to a variety of fields, including biomedical research and diagnostic pathology. The book begins with an overview of EM immunocytochemical procedures and of applications in histopathology. The three major chapters cover post-embedding, pre-embedding, and ultra-thin cryosection techniques. The final section illustrates applications of EM immunocytochemistry to the study of endocrine tumors, renal disease, neuropathology, dermatology, and microbiology. The emphasis throughout this detailed volume is on providing practical guidance to scientists and clinicians who may be using the technique for the first time.

Molecular Cellular Microbiology

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Author : Philippe Sansonetti
Publisher : Gulf Professional Publishing
ISBN 13 : 9780125215312
Total Pages : 638 pages
Book Rating : 4.2/5 (153 download)

Book Synopsis Molecular Cellular Microbiology by : Philippe Sansonetti

Download or read book Molecular Cellular Microbiology written by Philippe Sansonetti and published by Gulf Professional Publishing. This book was released on 2002-02-05 with total page 638 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume in the Methods in Microbiology series focuses on the interaction of microorganisms and the host cell, presenting detailed experimental techniques for modern microbiological research. The book focuses on current technical methods, including imaging technologies, cellular biochemistry, and the establishment and exploitation of cell assay systems. Also covered are methods for studying gene expression and detecting virulence genes. By studying the major techniques used to study cross talk between microbes and cells, rather than just presenting systems, this book distinguishes itself as an essential guide for all researchers working in microbiology, cell biology, and immunology. Key Features * Focuses on current technical methods, including imaging technologies, cellular biochemistry, establishment and exploitation of cell assay systems * Covers promising new areas such as global analysis of genome expression and proteomic analysis of cellular components. * Encompasses the most recent and innovative techniques such as microarrays, new experimental models of infection and new cell assay systems * Provides a large array of models covering the various strategies used by pathogens to infect their host * Includes all current methods developed to study gene expression and detect virulence genes.

Biomedical Electron Microscopy

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Author : Arvid B. Maunsbach
Publisher : Elsevier
ISBN 13 : 9780080528090
Total Pages : 548 pages
Book Rating : 4.5/5 (28 download)

Book Synopsis Biomedical Electron Microscopy by : Arvid B. Maunsbach

Download or read book Biomedical Electron Microscopy written by Arvid B. Maunsbach and published by Elsevier. This book was released on 1998-11-03 with total page 548 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive reference illustrates optimal preparation methods in biological electron microscopy compared with common methodological problems. Not only will the basic methodologies of transmission electron microscopy like fixation, microtomy, and microscopy be presented, but the authors also endeavor to illustrate more specialized techniques such as negative staining, autoradiography, cytochemistry, immunoelectron microscopy, and computer-assisted image analysis. Authored by the key leaders in the biological electron microscopy field Illustrates both optimal and suboptimal or artifactual results in a variety of electron microscopy disciplines Introduces students on how to read and interpret electron micrographs

Electron Microscopy of Plant Pathogens

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Author : Kurt Mendgen
Publisher : Springer Science & Business Media
ISBN 13 : 3642758185
Total Pages : 342 pages
Book Rating : 4.6/5 (427 download)

Book Synopsis Electron Microscopy of Plant Pathogens by : Kurt Mendgen

Download or read book Electron Microscopy of Plant Pathogens written by Kurt Mendgen and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 342 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plants, fungi, and viruses were among the first biological objects studied with an electron microscope. One of the two first instruments built by Siemens was used by Helmut Ruska, a brother of Ernst Ruska, the pioneer in constructing electron microscopes. H. Ruska published numerous papers on different biological objects in 1939. In one of these, the pictures by G. A. Kausche, E. Pfankuch, and H. Ruska of tobacco mosaic virus opened a new age in microscopy. The main problem was then as it still is today, to obtain an appropriate preparation of the specimen for observation in the electron microscope. Beam damage and specimen thickness were the first obstacles to be met. L. Marton in Brussels not only built his own instrument, but also made considerable progress in specimen preparation by introducing the impregnation of samples with heavy metals to obtain useful contrast. His pictures of the bird nest orchid root impregnated with osmium were revolutionary when published in 1934. It is not the place here to recall the different techniques which were developed in the subsequent years to attain the modern knowledge on the fine structure of plant cells and of different plant pathogens. The tremendous progress obtained with tobacco mosaic virus is reflected in the chapter by M. Wurtz on the fine structure of viruses in this Volume. New cytochemical and immunological techniques considerably surpass the morphological information obtained from the pathogens, especially at the host-parasite interface.

Electron Microscopy in Diagnostic Virology

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Author : Frances W. Doane
Publisher : CUP Archive
ISBN 13 : 9780521243117
Total Pages : 200 pages
Book Rating : 4.2/5 (431 download)

Book Synopsis Electron Microscopy in Diagnostic Virology by : Frances W. Doane

Download or read book Electron Microscopy in Diagnostic Virology written by Frances W. Doane and published by CUP Archive. This book was released on 1987-01-30 with total page 200 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Microbes as Tools for Cell Biology

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Author :
Publisher : Academic Press
ISBN 13 : 9780080859439
Total Pages : 339 pages
Book Rating : 4.8/5 (594 download)

Book Synopsis Microbes as Tools for Cell Biology by :

Download or read book Microbes as Tools for Cell Biology written by and published by Academic Press. This book was released on 1995-02-08 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microbes as Tools for Cell Biology bridges the gap between cell biology and microbiology. This laboratory guide provides a microbial tool kit for biologists who wish to use microbes as probes for basic cellular functions. The volume is organized into three sections, covering essential information on culture and genetic manipulation of microbes, assays for pathogen-host recognition, and analysis of intracellular parasitism. Each chapter outlines practical procedures and describes the rationale behind their development. This volume should prove useful to anyone interested in the biology of infectious agents, or their exploitation as a new generation of cell biological reagents. Key Features * Introduction by renowned microbiologist Dr. Stanley Falkow * Covers manipulation of pathogens, especially generation and selection of non-virulent phenotypes * Guides researchers in the study of intracellular pathogenesis * Describes microbial adherence and phagocytosis assays * Focuses on protein trafficking in infected cells * Well-illustrated with color plates, halftones, and diagrams

Methods in Plant Cell Biology

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Author : David W. Galbraith
Publisher : Academic Press
ISBN 13 : 0125641516
Total Pages : 605 pages
Book Rating : 4.1/5 (256 download)

Book Synopsis Methods in Plant Cell Biology by : David W. Galbraith

Download or read book Methods in Plant Cell Biology written by David W. Galbraith and published by Academic Press. This book was released on 1995 with total page 605 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Plant Cell Biology provides in two volumes a comprehensive collection of analytical methods essential for researchers and students in the plant sciences. Individual chapters, written by experts in the field, provide an introductory overview, followed by a step-by-step technical description of the methods. Key Features * Written by experts, many of whom have developed the individual methods described * Contains most, if not all, the methods needed for modern research in plant cell biology * Up-to-date and comprehensive * Full references * Allows quick access to relevant journal articles and to the sources of chemicals required for the procedures * Selective concentration on higher plant methods allows for particular emphasis on those problems specific to plants.

Methods in Plant Electron Microscopy and Cytochemistry

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Author : William V. Dashek
Publisher : Springer Science & Business Media
ISBN 13 : 1592592325
Total Pages : 297 pages
Book Rating : 4.5/5 (925 download)

Book Synopsis Methods in Plant Electron Microscopy and Cytochemistry by : William V. Dashek

Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.

Cumulated Index Medicus

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Author :
Publisher :
ISBN 13 :
Total Pages : 1840 pages
Book Rating : 4.3/5 (243 download)

Book Synopsis Cumulated Index Medicus by :

Download or read book Cumulated Index Medicus written by and published by . This book was released on 2000 with total page 1840 pages. Available in PDF, EPUB and Kindle. Book excerpt: