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Author : R. Bruce Dunlap
Publisher :
ISBN 13 :
Total Pages : 377 pages
Book Rating : 4.:/5 (91 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography written by R. Bruce Dunlap and published by . This book was released on 1974 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : R Dunlap
Publisher : Springer
ISBN 13 : 9781468469837
Total Pages : 392 pages
Book Rating : 4.4/5 (698 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography written by R Dunlap and published by Springer. This book was released on 2014-01-15 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : R. Dunlap
Publisher : Springer Science & Business Media
ISBN 13 : 1468469827
Total Pages : 372 pages
Book Rating : 4.4/5 (684 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography written by R. Dunlap and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains most of the papers presented at the Sym posium on Affinity Chromatography and Immobilized Biochemicals, which was held on November 7-9, 1973 in Charleston, South Carolina in conjunction with the Southeastern Regional American Chemical Society meeting. The topics of the symposium represent two new biochemical frontiers which have emerged in recent years through the ingenious development and application of solid phase biochemi cal technologies. Affinity chromatography involves the use of selected ligands, covalently bound to a solid support such as cel lulose, glass, Sepharose, or polyacrylamide and exploits the princi ple of biochemical recognition between the ligand in the solid phase and a selected macromolecule to facilitate the rapid and often quantitative purification of enzymes, antibodies, antigens, hormones, other proteins, etc. The area of immobilized biochemicals includes the use of coenzymes, oligo- and po1ynuc1eotides, enzymes, and multistep enzyme systems which are immobilized or entrapped in the solid phase. The goals of the symposium were the revi~w of the status of affinity chromatography and immobilized biochemicals, the presentation of new data and ideas in both areas, and the establish ment of a dialogue between research workers in these two evolving disciplines which are so closely interrelated. The papers published in this volume provide the reader with reviews of several topics inherent in the solid phase biochemistry together with a series of timely manuscripts concerning new techniques and applications both in the use of affinity chromatography and in the investigation of immobilized biochemicals.
Author : Pascal Bailon
Publisher : Springer Science & Business Media
ISBN 13 : 1603272615
Total Pages : 233 pages
Book Rating : 4.6/5 (32 download)
Download or read book Affinity Chromatography written by Pascal Bailon and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 233 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography, with its exquisite specificity, is based upon molecular recognition. It is a powerful tool for the purification of biomolecules. In recent years, numerous new applications and modified techniques have been derived from gro- specific interactions and biological recognition principles. An up-to-date review of the past, current, and future applications of affinity chromatography has been presented in the introductory chapter by Meir Wilchek and Irwin Chaiken. Though many of these new applications and techniques are well documented in the literature, it is often difficult to find methods that are written with the intent of helping new practitioners of affinity chromatography. This volume on Affinity Chromatography: Methods and Protocols is intended for the novice, as well as for - perts in the field. The protocols are written by experts who have developed and/or successfully employed these methods in their laboratories. Each chapter describes a specific technique, and since the book is intended to help the beginner, each technique is described simply and clearly, making sure that all relevant steps are included, assuming no previous knowledge. Each chapter contains an introduction describing the principles involved, followed by a Materials and Methods section, which lays the groundwork for the reader to conduct experiments step-by-step, in an orderly fashion. The following Notes section, which describes many of the problems that can occur, makes suggestions for overcoming them, and provides alternate procedures. These are precisely the sort of important, practical details that never seem to appear in the published literature.
Author : Greg T. Hermanson
Publisher : Academic Press
ISBN 13 :
Total Pages : 494 pages
Book Rating : 4.F/5 ( download)
Download or read book Immobilized Affinity Ligand Techniques written by Greg T. Hermanson and published by Academic Press. This book was released on 1992-08-27 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a practical guide to the preparation and use of immobilized affinity ligands for purification, catalysis, and analysis. Special emphasis is given to immunochemical techniques including antibody isolation, preparation of antibody fragments using immobilized enzymes, and immunoaffinity chromatography. The book provides easy-to-follow, well-tested protocols to allow the uninitiated to use these techniques to the maximum advantage with minimum hassle. In addition, it shows researchers how to save money by making their own optimized affinity supports. Matrix activation: Ligand immobilization, Binding and elution of target molecules, Enzyme catalysis on solid supports, Analytical affinity chromatography, Isolation/purification of antibodies, Preparation of antibody fragments, Immunoaffinity chromatography, Immobilization of nucleic acids, Use of immobilized ligands for removal of trace contaminants Practical advice on choosing: Matrices, Spacers, Methods of activation and coupling Background information and insights on: Affinity interactions, The ease and power of affinity chromatography, Attaching molecules to insoluble supports, Matrices currently in use, Over 20 methods of activation, Spacers, Extensive References
Author : Toni Kline
Publisher : CRC Press
ISBN 13 : 1482285010
Total Pages : 1198 pages
Book Rating : 4.4/5 (822 download)
Download or read book Handbook of Affinity Chromatography written by Toni Kline and published by CRC Press. This book was released on 1993-06-16 with total page 1198 pages. Available in PDF, EPUB and Kindle. Book excerpt: Outlining the fundamental principles by which all interactions occur, this reference focuses on harnessing the biochemistry of bioorganic compounds in order to separate them, presenting new techniques and applications that affect the planning of research strategies. The contributors discuss how to c
Author : Jan-Christer Janson
Publisher : John Wiley & Sons
ISBN 13 : 9780471186267
Total Pages : 712 pages
Book Rating : 4.1/5 (862 download)
Download or read book Protein Purification written by Jan-Christer Janson and published by John Wiley & Sons. This book was released on 1998 with total page 712 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a state-of-the-art sourcebook on modern high-resolution biochemical separation techniques for proteins. It contains all the basic theory and principles used in protein chromatography and electrophoresis.
Author : Irwin Chaiken
Publisher : Elsevier
ISBN 13 : 0323146627
Total Pages : 542 pages
Book Rating : 4.3/5 (231 download)
Download or read book Affinity Chromatography and Biological Recognition written by Irwin Chaiken and published by Elsevier. This book was released on 2012-12-02 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography and Biological Recognition contains manuscripts presented at the Fifth International Symposium on Affinity Chromatography and Biological Recognition convened in June 12-17, 1983, at St. John's College in Annapolis, Maryland. Organized into six parts encompassing 82 chapters, the book begins by examining the growing synergism between affinity methods and the understanding and study of basic principles of biological recognition. The book then focuses on the trends and progress in the design and application of affinity methods for isolation, therapeutics, diagnostics, and biotechnology. Significant chapters are devoted to the contributions of affinity methodology in such areas as cell membrane receptors, quantitative properties of macromolecular interactions, microscale analytical and preparative applications of high performance affinity chromatography, antibodies as in vivo and in vitro diagnostic and therapeutic agents, and drug targeting. This volume will be a stimulus for broad and creative application of affinity concepts and methods in many fields of biomedical research and biotechnology.
Author : R. Dunlap
Publisher : Springer
ISBN 13 : 9781468469844
Total Pages : 0 pages
Book Rating : 4.4/5 (698 download)
Download or read book Immobilized Biochemicals and Affinity Chromatography written by R. Dunlap and published by Springer. This book was released on 2012-06-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains most of the papers presented at the Sym posium on Affinity Chromatography and Immobilized Biochemicals, which was held on November 7-9, 1973 in Charleston, South Carolina in conjunction with the Southeastern Regional American Chemical Society meeting. The topics of the symposium represent two new biochemical frontiers which have emerged in recent years through the ingenious development and application of solid phase biochemi cal technologies. Affinity chromatography involves the use of selected ligands, covalently bound to a solid support such as cel lulose, glass, Sepharose, or polyacrylamide and exploits the princi ple of biochemical recognition between the ligand in the solid phase and a selected macromolecule to facilitate the rapid and often quantitative purification of enzymes, antibodies, antigens, hormones, other proteins, etc. The area of immobilized biochemicals includes the use of coenzymes, oligo- and po1ynuc1eotides, enzymes, and multistep enzyme systems which are immobilized or entrapped in the solid phase. The goals of the symposium were the revi~w of the status of affinity chromatography and immobilized biochemicals, the presentation of new data and ideas in both areas, and the establish ment of a dialogue between research workers in these two evolving disciplines which are so closely interrelated. The papers published in this volume provide the reader with reviews of several topics inherent in the solid phase biochemistry together with a series of timely manuscripts concerning new techniques and applications both in the use of affinity chromatography and in the investigation of immobilized biochemicals.
Author : Sameh Magdeldin
Publisher : BoD – Books on Demand
ISBN 13 : 9535103253
Total Pages : 360 pages
Book Rating : 4.5/5 (351 download)
Download or read book Affinity Chromatography written by Sameh Magdeldin and published by BoD – Books on Demand. This book was released on 2012-03-21 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Most will agree that one major achievement in the bio-separation techniques is affinity chromatography. This coined terminology covers a myriad of separation approaches that relies mainly on reversible adsorption of biomolecules through biospecific interactions on the ligand. Within this book, the authors tried to deliver for you simplified fundamentals of affinity chromatography together with exemplarily applications of this versatile technique. We have always been endeavor to keep the contents of the book crisp and easily comprehensive, hoping that this book will receive an overwhelming interest, deliver benefits and valuable information to the readers.
Author : Herbert Schott
Publisher : CRC Press
ISBN 13 : 1000110583
Total Pages : 249 pages
Book Rating : 4.0/5 (1 download)
Download or read book Affinity Chromatography written by Herbert Schott and published by CRC Press. This book was released on 2020-11-25 with total page 249 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book informs the reader about the practical methods, possibilities, and limits of template chromatography. It shows the various techniques for immobilization of nucleic acids fragments, polynucleotides, and nucleic acids by which the desired separation of materials can be achieved.
Author : Munishwar N. Gupta
Publisher : Birkhäuser
ISBN 13 : 3034881274
Total Pages : 234 pages
Book Rating : 4.0/5 (348 download)
Download or read book Methods for Affinity-Based Separations of Enzymes and Proteins written by Munishwar N. Gupta and published by Birkhäuser. This book was released on 2013-12-01 with total page 234 pages. Available in PDF, EPUB and Kindle. Book excerpt: One major concern of biotechnology is either using enzymes or producing them. Enzyme/protein production is therefore an important starting point for biotechnology. Bioseparation or Downstream Processing constitutes about 40-90% of the total production cost. Driven by economics, highly selective technologies applicable to large-scale processing have emerged during the last decade. These technologies are slowly diffusing to enzymologists who are working on a smaller scale, looking for fast and efficient purification protocols. The affinity-based techniques (including precipitation, two-phase extractions, expanded bed chromatography, perfusion chromatography and monoliths) described in this volume provide current and new cutting-edge methods. Consequently, the book is of main interest to researchers in biochemistry, biochemical engineering and biotechnology, working either in academic or industrial sectors.
Author : D.S. Hage
Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128071060
Total Pages : 688 pages
Book Rating : 4.1/5 (28 download)
Download or read book Liquid Chromatography written by D.S. Hage and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 688 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity chromatography is a type of liquid chromatography that uses a biologically related agent as a stationary phase to purify or analyze specific sample components. This chapter examines the basic components and principles of affinity chromatography and various applications of this method. The general types of ligands, immobilization schemes, support materials, and elution conditions that can be employed in affinity chromatography are first examined. Various methods in this field are then considered, including bioaffinity chromatography, immunoaffinity chromatography, dye-ligand and biomimetic affinity chromatography, immobilized metal-ion affinity chromatography, and analytical affinity chromatography. The types of ligands utilized in each of these methods are described, along with common applications of these techniques. Several related developments are also discussed, such as the use of affinity chromatography with mass spectrometry, miniaturized systems, molecularly imprinted polymers, and aptamers.
Author : Irwin M. Chaiken
Publisher : CRC Press
ISBN 13 : 1351078127
Total Pages : 204 pages
Book Rating : 4.3/5 (51 download)
Download or read book Analytical Affinity Chromatography written by Irwin M. Chaiken and published by CRC Press. This book was released on 2018-01-10 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents discussions of theoretical and experimental considerations that have led to the analytical affinity chromatography field, as well as current efforts to use this methodology to characterize the interaction mechanisms of biological macromolecules and to establish conditions for employing bioaffinity chromatographic systems as preparative tools. The chapters include a comprehensive discussion of interactive chromatography theory (DeLisi and Hethcote), a review of experimental data obtained for biological macromolecules and the relevant theoretical considerations of affinity chromatography which led to them (Swaisgood and Chaiken), an evaluation of rate processes in affinity chromatography and the potential to determine biologically meaningful chemical rate constants (Walters), and the use of quantitative and molecular considerations to design affinity chromatographic systems (Stellwagen and Liu).
Author : David S. Hage
Publisher : CRC Press
ISBN 13 : 0824751981
Total Pages : 878 pages
Book Rating : 4.8/5 (247 download)
Download or read book Handbook of Affinity Chromatography written by David S. Hage and published by CRC Press. This book was released on 2005-07-19 with total page 878 pages. Available in PDF, EPUB and Kindle. Book excerpt: This essential handbook guides investigators in the theory, applications, and practical use of affinity chromatography in a variety of fields including biotechnology, biochemistry, molecular biology, analytical chemistry, proteomics, pharmaceutical science, environmental analysis, and clinical chemistry. The Handbook of Affinity Chromatograph
Author : O. Hoffmann-Ostenhof
Publisher : Elsevier
ISBN 13 : 1483188361
Total Pages : 391 pages
Book Rating : 4.4/5 (831 download)
Download or read book Affinity Chromatography written by O. Hoffmann-Ostenhof and published by Elsevier. This book was released on 2016-01-22 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Affinity Chromatography is a collection of papers that presents the advancement in various areas of affinity chromatography. The title particularly covers the development in affinity chromatography in the context of biochemistry and immunochemistry. The text first details gel matrices, coupling methods, and charge transfer chromatography. Next, the selection tackles the affinity chromatography of enzymes and hydrophobic interaction chromatography. The text also discusses matrix-bound antigens and antibodies, along with matrix-bound lectins and affinity chromatography of cells. Part VI deals with affinity chromatography of hormone receptors, while part vii talks about affinity techniques in the isolation Of DNA, RNA, and polysomes. The book will be of great use to students, researchers, and practitioners of biochemistry.
Author : R O Okotore
Publisher : New Age International
ISBN 13 : 9788122411706
Total Pages : 108 pages
Book Rating : 4.4/5 (117 download)
Download or read book Basic Separation Techniques in Biochemistry written by R O Okotore and published by New Age International. This book was released on 1998 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Basic Separation Techniques In Biochemistry Provides Information On The Basic Separation Techniques Most Commonly Employed In Biochemical Research.The Basic Principles And Applications Of The Routine Methods For The Fractionation Of Subcellular Macromolecules Have Been Discussed In Simple And Comprehensive Manner.The Methodology Of Each Technique Is Presented In A Precise And Concise Way For Meaningful Understanding To A Beginner Student. The Book Is In Eight Chapters, Each With Statement Of Objectives. The Book Will Prove Of Value To Undergraduate Students Of Biochemistry, Chemistry And Biology As Supplementary Reading Text To More Advanced Texts In Laboratory Techniques.
