Imaging Flow Cytometry

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Imaging Flow Cytometry

Author : Natasha S. Barteneva
Publisher : Humana
ISBN 13 : 9781493933006
Total Pages : 0 pages
Book Rating : 4.9/5 (33 download)

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Book Synopsis Imaging Flow Cytometry by : Natasha S. Barteneva

Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by Humana. This book was released on 2015-11-23 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume for the first time explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionized our ability to analyze cells, cellular clusters, and populations in a remarkable fashion. Beginning with an introduction to technology, the book continues with sections addressing protocols for studies on the cell nucleus, nucleic acids, and FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Imaging Flow Cytometry: Methods and Protocols will be a critical source for all laboratories seeking to implement IFC in their research studies.

Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies

Author : Anna Porwit
Publisher : Cambridge University Press
ISBN 13 : 1108548830
Total Pages : 248 pages
Book Rating : 4.1/5 (85 download)

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Book Synopsis Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies by : Anna Porwit

Download or read book Multiparameter Flow Cytometry in the Diagnosis of Hematologic Malignancies written by Anna Porwit and published by Cambridge University Press. This book was released on 2018-01-25 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: Master implementation of the techniques of flow cytometry in diagnosing complex haematological diseases and malignancies in patients, worldwide. Featuring World Health Organization recommendations on pre-analytical steps, instrument settings and panel construction, this invaluable manual offers invaluable support for those researching, practising and analyzing the cause of hematological malignancies. Authored by leading experts, this book puts flow-cytometry into everyday context. With a focus on multicolour panels, the manual provides readers an experienced understanding of effective, implementation techniques. Practitioners of all levels are offered a background in a variety of diseases presented alongside the most current methodology. Wide-ranging and comprehensive; detailed images of healthy blood, bone marrow and lymph-nodes are illustrated throughout, allowing for effective diagnosis. Through engaging with differential diagnoses, the manual offers an understanding of similar symptoms and mimicking malignancies, avoiding inaccurate results. Featuring in-depth descriptions of chronic diseases; users can reach accurate diagnosis, first time.

Flow Cytometry

Author : Alice Longobardi Givan
Publisher : John Wiley & Sons
ISBN 13 : 1118688392
Total Pages : 309 pages
Book Rating : 4.1/5 (186 download)

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Book Synopsis Flow Cytometry by : Alice Longobardi Givan

Download or read book Flow Cytometry written by Alice Longobardi Givan and published by John Wiley & Sons. This book was released on 2013-04-10 with total page 309 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry continually amazes scientists with its ever-expanding utility. Advances in flow cytometry have opened new directions in theoretical science, clinical diagnosis, and medical practice. The new edition of Flow Cytometry: First Principles provides a thorough update of this now classic text, reflecting innovations in the field while outlining the fundamental elements of instrumentation, sample preparation, and data analysis. Flow Cytometry: First Principles, Second Edition explains the basic principles of flow cytometry, surveying its primary scientific and clinical applications and highlighting state-of-the-art techniques at the frontiers of research. This edition contains extensive revisions of all chapters, including new discussions on fluorochrome and laser options for multicolor analysis, an additionalsection on apoptosis in the chapter on DNA, and new chapters onintracellular protein staining and cell sorting, including high-speed sorting and alternative sorting methods, as well as traditional technology. This essential resource: Assumes no prior knowledge of flow cytometry Progresses with an informal, engaging lecture style from simpleto more complex concepts Offers a clear introduction to new vocabulary, principles of instrumentation, and strategies for data analysis Emphasizes the theory relevant to all flow cytometry, with examples from a variety of clinical and scientific fields Flow Cytometry: First Principles, Second Edition provides scientists, clinicians, technologists, and students with the knowledge necessary for beginning the practice of flow cytometry and for understanding related literature.

Imaging Flow Cytometry

Author : Natasha S. Barteneva
Publisher :
ISBN 13 : 9781493933020
Total Pages : 295 pages
Book Rating : 4.9/5 (33 download)

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Book Synopsis Imaging Flow Cytometry by : Natasha S. Barteneva

Download or read book Imaging Flow Cytometry written by Natasha S. Barteneva and published by . This book was released on 2015 with total page 295 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume explores techniques and protocols involving quantitative imaging flow cytometry (IFC), which has revolutionised our ability to analyse cells, cellular clusters and populations. Beginning with an introduction to technology, it continues with sections addressing protocols for studies on the cell nucleus and nucleic acids, FISH techniques using an IFC instrument, immune response analysis and drug screening, IFC protocols for apoptosis and cell death analysis, as well as morphological analysis and the identification of rare cells.

The Microflow Cytometer

Author : Frances S. Ligler
Publisher : Pan Stanford Publishing
ISBN 13 : 9814267414
Total Pages : 394 pages
Book Rating : 4.8/5 (142 download)

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Book Synopsis The Microflow Cytometer by : Frances S. Ligler

Download or read book The Microflow Cytometer written by Frances S. Ligler and published by Pan Stanford Publishing. This book was released on 2010-05-31 with total page 394 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Great book! Excellent compilation. From history of the very early days of flow cytometers to the latest unique unconventional microflow cytometers. From commercialization philosophy to cutting edge engineering designs. From fluid mechanics to optics to electronic circuit considerations. Well balanced and comprehensive."--Shuichi Takayama University of Michigan, USA.

Flow Cytometry Protocols

Author : Teresa S. Hawley
Publisher : Springer Science & Business Media
ISBN 13 : 1592597734
Total Pages : 436 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Flow Cytometry Protocols by : Teresa S. Hawley

Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Flow cytometry has evolved since the 1940s into a multidisciplinary field incorporating aspects of laser technology, fluid dynamics, electronics, optics, computer science, physics, chemistry, biology, and mathematics. Innovations in instrumentation, development of small lasers, discovery of new fluorochromes/fluorescent proteins, and implementation of novel methodologies have all contributed to the recent rapid expansion of flow cytometry applications. In this thoroughly revised and updated second edition of Flow Cytometry Protocols, time-proven as well as cutting-edge methods are clearly and comprehensively presented by leading experimentalists. In addition to being a valuable reference manual for experienced flow cytometrists, the editors expect this authoritative up-to-date collection to prove useful to investigators in all areas of the biological and biomedical sciences who are new to the subject. The introductory chapter provides an eloquent synopsis of the principles and diverse uses of flow cytometry, beginning with a historical perspective and ending with a view to the future. Chapters 2–22 contain step-by-step protocols of highly practical and state-of-the-art techniques. Detailed instructions and helpful tips on experimental design, as well as selection of reagents and data analysis tools, will allow researchers to readily carry out flow cytometric investigations ranging from traditional phenotypic characterizations to emerging genomics and proteomics applications. Complementing these instructive protocols is a chapter that provides a preview of the next generation of solid-state lasers, and one that describes a rapid means to validate containment of infectious aerosols generated during high-speed sorting (Chapters 23–24).

Advanced Optical Flow Cytometry

Author : Valery V. Tuchin
Publisher : John Wiley & Sons
ISBN 13 : 3527634290
Total Pages : 698 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Advanced Optical Flow Cytometry by : Valery V. Tuchin

Download or read book Advanced Optical Flow Cytometry written by Valery V. Tuchin and published by John Wiley & Sons. This book was released on 2011-03-31 with total page 698 pages. Available in PDF, EPUB and Kindle. Book excerpt: A detailed look at the latest research in non-invasive in vivo cytometry and its applications, with particular emphasis on novel biophotonic methods, disease diagnosis, and monitoring of disease treatment at single cell level in stationary and flow conditions. This book thus covers the spectrum ranging from fundamental interactions between light, cells, vascular tissue, and cell labeling particles, to strategies and opportunities for preclinical and clinical research. General topics include light scattering by cells, fast video microscopy, polarization, laser-scanning, fluorescence, Raman, multi-photon, photothermal, and photoacoustic methods for cellular diagnostics and monitoring of disease treatment in living organisms. Also presented are discussions of advanced methods and techniques of classical flow cytometry.

Flow Cytometry Basics for the Non-Expert

Author : Christine Goetz
Publisher : Springer
ISBN 13 : 3319980718
Total Pages : 229 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis Flow Cytometry Basics for the Non-Expert by : Christine Goetz

Download or read book Flow Cytometry Basics for the Non-Expert written by Christine Goetz and published by Springer. This book was released on 2018-11-08 with total page 229 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first edition volume demystifies the complex topic of flow cytometry by providing detailed explanations and nearly 120 figures to help novice flow cytometry users learn and understand the bedrock principles necessary to perform basic flow cytometry experiments correctly. The book divides the topic of flow cytometry into easy to understand sections and covers topics such as the physics behind flow cytometry, flow cytometry lingo, designing flow cytometry experiments and choosing appropriate fluorochromes, compensation, sample preparation and controls and ways to assess cellular function using a variety of flow cytometry assays. Written as a series of chapters whose concepts sequentially build off one another, using the list of materials contained within each section along with the readily reproducible laboratory protocols and tips on troubleshooting that are included, readers should be able to reproduce the data figures presented throughout the book on their way to mastering sound basic flow cytometry techniques. Easy to understand and comprehensive, Flow Cytometry Basics for the Non-Expert will be a valuable resource to novice flow cytometry users as well as experts in other biomedical research fields who need to familiarize themselves with a basic understanding of how to perform flow cytometry and interpret flow cytometry data. This book is written for both scientists and non-scientists in academia, government, biotechnology, and medicine.

Shape, Contour and Grouping in Computer Vision

Author : David A. Forsyth
Publisher : Springer Science & Business Media
ISBN 13 : 3540667229
Total Pages : 340 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis Shape, Contour and Grouping in Computer Vision by : David A. Forsyth

Download or read book Shape, Contour and Grouping in Computer Vision written by David A. Forsyth and published by Springer Science & Business Media. This book was released on 1999-11-03 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Computer vision has been successful in several important applications recently. Vision techniques can now be used to build very good models of buildings from pictures quickly and easily, to overlay operation planning data on a neuros- geon’s view of a patient, and to recognise some of the gestures a user makes to a computer. Object recognition remains a very di cult problem, however. The key questions to understand in recognition seem to be: (1) how objects should be represented and (2) how to manage the line of reasoning that stretches from image data to object identity. An important part of the process of recognition { perhaps, almost all of it { involves assembling bits of image information into helpful groups. There is a wide variety of possible criteria by which these groups could be established { a set of edge points that has a symmetry could be one useful group; others might be a collection of pixels shaded in a particular way, or a set of pixels with coherent colour or texture. Discussing this process of grouping requires a detailed understanding of the relationship between what is seen in the image and what is actually out there in the world.

Flow Cytometry and Cell Sorting

Author : Andreas Radbruch
Publisher : Springer Science & Business Media
ISBN 13 : 3662041294
Total Pages : 365 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Flow Cytometry and Cell Sorting by : Andreas Radbruch

Download or read book Flow Cytometry and Cell Sorting written by Andreas Radbruch and published by Springer Science & Business Media. This book was released on 2013-03-14 with total page 365 pages. Available in PDF, EPUB and Kindle. Book excerpt: The analysis and sorting of large numbers of cells with a fluorescence-activated cell sorter (FACS) was first achieved some 30 years ago. Since then, this technology has been rapidly developed and is used today in many laboratories. A Springer Lab Manual Review of the First Edition: "This is a most useful volume which will be a welcome addition for personal use and also for laboratories in a wide range of disciplines. Highly recommended." CYTOBIOS

Cell Interaction

Author : Sivakumar Joghi Thatha Gowder
Publisher : BoD – Books on Demand
ISBN 13 : 9535107925
Total Pages : 336 pages
Book Rating : 4.5/5 (351 download)

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Book Synopsis Cell Interaction by : Sivakumar Joghi Thatha Gowder

Download or read book Cell Interaction written by Sivakumar Joghi Thatha Gowder and published by BoD – Books on Demand. This book was released on 2012-10-10 with total page 336 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book Cell Interaction focuses on various processes that occur within and outside the cells. Cell interactions are important for functioning of many organ systems: cell adhesion, tissue development, cellular communication, inflammation, tumor metastasis, and microbial infection. Key features include developmental cell interactions, immune and neural cell interactions, cell interactions in normal and disease conditions and advanced level methods to evaluate cell interactions. This book will be a significant resource to all scientists and physicians who are intended to explore more on cells.

Practical Flow Cytometry

Author : Howard M. Shapiro
Publisher : John Wiley & Sons
ISBN 13 : 0471434035
Total Pages : 736 pages
Book Rating : 4.4/5 (714 download)

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Book Synopsis Practical Flow Cytometry by : Howard M. Shapiro

Download or read book Practical Flow Cytometry written by Howard M. Shapiro and published by John Wiley & Sons. This book was released on 2005-02-25 with total page 736 pages. Available in PDF, EPUB and Kindle. Book excerpt: From the reviews of the 3rd Edition... "The standard reference for anyone interested in understandingflow cytometry technology." American Journal of Clinical Oncology "...one of the most valuable of its genre and...addressed to awide audience?written in such an attractive way, being bothinformative and stimulating." Trends in Cell Biology This reference explains the science and discusses the vastbiomedical applications of quantitative analytical cytology usinglaser-activated detection and cell sorting. Now in its fourthedition, this text has been expanded to provide full coverage ofthe broad spectrum of applications in molecular biology andbiotechnology today. New to this edition are chapters on automatedanalysis of array technologies, compensation, high-speed sorting,reporter molecules, and multiplex and apoptosis assays, along withfully updated and revised references and a list of suppliers.

Artificial Intelligence in Healthcare and Medicine

Author : Kayvan Najarian
Publisher : CRC Press
ISBN 13 : 1000565815
Total Pages : 300 pages
Book Rating : 4.0/5 (5 download)

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Book Synopsis Artificial Intelligence in Healthcare and Medicine by : Kayvan Najarian

Download or read book Artificial Intelligence in Healthcare and Medicine written by Kayvan Najarian and published by CRC Press. This book was released on 2022-04-06 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a comprehensive overview of the recent developments in clinical decision support systems, precision health, and data science in medicine. The book targets clinical researchers and computational scientists seeking to understand the recent advances of artificial intelligence (AI) in health and medicine. Since AI and its applications are believed to have the potential to revolutionize healthcare and medicine, there is a clear need to explore and investigate the state-of-the-art advancements in the field. This book provides a detailed description of the advancements, challenges, and opportunities of using AI in medical and health applications. Over 10 case studies are included in the book that cover topics related to biomedical image processing, machine learning for healthcare, clinical decision support systems, visualization of high dimensional data, data security and privacy, bioinformatics, and biometrics. The book is intended for clinical researchers and computational scientists seeking to understand the recent advances of AI in health and medicine. Many universities may use the book as a secondary training text. Companies in the healthcare sector can greatly benefit from the case studies covered in the book. Moreover, this book also: Provides an overview of the recent developments in clinical decision support systems, precision health, and data science in medicine Examines the advancements, challenges, and opportunities of using AI in medical and health applications Includes 10 cases for practical application and reference Kayvan Najarian is a Professor in the Department of Computational Medicine and Bioinformatics, Department of Electrical Engineering and Computer Science, and Department of Emergency Medicine at the University of Michigan, Ann Arbor. Delaram Kahrobaei is the University Dean for Research at City University of New York (CUNY), a Professor of Computer Science and Mathematics, Queens College CUNY, and the former Chair of Cyber Security, University of York. Enrique Domínguez is a professor in the Department of Computer Science at the University of Malaga and a member of the Biomedical Research Institute of Malaga. Reza Soroushmehr is a Research Assistant Professor in the Department of Computational Medicine and Bioinformatics and a member of the Michigan Center for Integrative Research in Critical Care, University of Michigan, Ann Arbor.

Imaging Flow Cytometer and Image-Activated Single Cell Dispensing Platform with Artificial Intelligence

Author : Zunming Zhang
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (138 download)

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Book Synopsis Imaging Flow Cytometer and Image-Activated Single Cell Dispensing Platform with Artificial Intelligence by : Zunming Zhang

Download or read book Imaging Flow Cytometer and Image-Activated Single Cell Dispensing Platform with Artificial Intelligence written by Zunming Zhang and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of imaging flow cytometry (IFC) technology in high-throughput cellular image processing and analysis can provide valuable insights into the fields of biology and medicine. The ability to classify, map, and isolate cells based on high-content cellular images is a powerful tool for researchers and medical professionals to better understand the connection between the phototype and genotype among heterogeneous cell populations. This dissertation outlines the methodology for conducting high-throughput cellular image analysis and single cell isolation using the 3D-IFC based single cell dispensing platform. Our work demonstrated the workflow for conducting a high-throughput label-free cell study using IFC systems and artificial intelligence. Additionally, we extended the system capability by incorporating the unsupervised learning and improving the interpretability of the machine learning model performance. Our work forms an unprecedented bridge between single-cell molecular analysis and single-cell image analysis to connect phenotype and genotype analysis with single-cell resolution. This system provides a new avenue for conducting cellular analysis and sorting based on interpretable machine vision and artificial intelligence.

Spectral and Imaging Cytometry

Author : Natasha S. Barteneva
Publisher : Springer Nature
ISBN 13 : 1071630202
Total Pages : 265 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Spectral and Imaging Cytometry by : Natasha S. Barteneva

Download or read book Spectral and Imaging Cytometry written by Natasha S. Barteneva and published by Springer Nature. This book was released on 2023-04-19 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition volume expands on the previous edition with discussions on the latest approaches used in the spectral cytometry field. Beginning with a brief history of spectral cytometry development, this book continues with a section addressing new protocols in different areas of imaging cytometry based on the unique technology of Imagestream and also introduces FlowCam – cytometer with capabilities of analyzing phytoplankton and zooplankton. Written for the highly successful Methods in Molecular Biology series, chapters include brief introductions to their respective topics, complete lists of the materials and reagents necessary, and reproducible laboratory protocols with tips on troubleshooting and recommendations. Cutting-edge and thorough, Spectral and Imaging Flow Cytometry: Methods and Protocols, Second Edition is a valuable resources for beginners and professionals interested in learning more about this developing field. Chapters 1, 2, and 5 are available open access under a Creative Commons Attribution 4.0 International License via link.springer.com.

Cellular Diagnostics

Author : Ulrich Sack
Publisher : Karger Medical and Scientific Publishers
ISBN 13 : 3805585551
Total Pages : 764 pages
Book Rating : 4.8/5 (55 download)

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Book Synopsis Cellular Diagnostics by : Ulrich Sack

Download or read book Cellular Diagnostics written by Ulrich Sack and published by Karger Medical and Scientific Publishers. This book was released on 2009-01-01 with total page 764 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is the updated English version of the 2006 German bestseller Zellulare Diagnostik, a comprehensive presentation of flow cytometry and its applications. While some techniques of immunophenotyping by flow cytometry already are routine procedures in the laboratory, new methods for the functional characterization of cells, the analysis of rare cells, and the diagnosis of complex materials have only begun to win wide recognition. New approaches such as slide-based cytometry will lead to an increase in the use of cytometric techniques. Multiparameter approaches will further improve analysis. The book provides a comprehensive and detailed compilation of all aspects of flow cytometry in research and the clinic. For newcomers it offers a thorough introduction, for advanced users, specific protocols and interpretation assistance.

Immunophenotyping

Author : J Philip McCoy Jr
Publisher : Humana
ISBN 13 : 9781493996490
Total Pages : 375 pages
Book Rating : 4.9/5 (964 download)

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Book Synopsis Immunophenotyping by : J Philip McCoy Jr

Download or read book Immunophenotyping written by J Philip McCoy Jr and published by Humana. This book was released on 2019-09-29 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the latest collection of immunophenotypic techniques and applications used in research and clinical settings. Chapters in this book cover topics such as constructions of high dimensions fluorescence and mass cytometry panels; fluorescence barcoding; using dried or lyophilized reagents; and immunophenotypic examples of specific cell types. The book concludes with a discussion on the critical roles of quality control and immunophenotyping in the clinical environment. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, Immunophenotyping: Methods and Protocols is a valuable resource for any researchers, clinician, or scientist interested in learning more about this evolving field.