Hydrocarbon And Lipid Microbiology Protocols

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Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662491294
Total Pages : 0 pages
Book Rating : 4.4/5 (912 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2016-04-22 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents relevant single-cell and single-molecule approaches in the study of microbes producing and utilizing hydrocarbons and lipids. While generically applicable for all microorganisms, the approaches described are, wherever possible, adapted to the field of study of hydrocarbon and lipid microbiology. The methods include basic procedures for isolating single cells by means of microfluidics and flow cytometry, and their cultivation in arrays as pure clones; for isolating, amplifying and sequencing single-cell genomes and transcriptomes; and for analysing single-cell metabolomes by means of Raman spectroscopy. Single-molecule approaches include the use of protein:fluorescent dye fusions for protein localization and methods for the production of cell division protostructures and lipid monolayers. Methods for the functional analysis of single cells include detection of metabolically active (protein-synthesizing) cells in environmental samples by bioorthogonal non-canonical amino acid tagging, Raman spectroscopy combined with stable isotope labelling and fluorescent in situ hybridisation, and visualization of single cells participating in gene transfer activity. Lastly, protocols are presented for single-cell biotechnological applications, including biofuel production. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662571149
Total Pages : 256 pages
Book Rating : 4.5/5 (711 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2018-12-30 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume covers protocols for various applications in hydrocarbon microbiology, including those of interest for industrial processes, biocatalysis, lipid and biofuel production, bioproducts, or the human microbiome. It presents detailed protocols for the functional screening of enzymes acting on greasy molecules (i.e. lipases, esterases), including assays for enantioselective biocatalysts, as well as approaches for protein display technologies. Protocols for improving fuel quality and production of biofuel and lipids in different hosts (bacteria, algae, yeast) are also provided. The production of biogas from organic waste and its fermentation into value-added products such as polyhydroxyalkanoates is covered, as well as an in-vitro model of the gut microbiome for short-chain fatty acid metabolism and microbial diversity analyses. The applications presented are examples of the many potential applications in hydrocarbon and lipid microbiology, and many (i.e. protein-display technologies) will also be of interest in other research fields. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662569856
Total Pages : 256 pages
Book Rating : 4.5/5 (698 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2018-12-09 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume features protocols for investigating the hydrocarbon- and lipid-specific activities of microbes. They include methods for studying chemotaxis, the colonisation of hydrocarbon surfaces, hydrocarbon uptake, respiration, nitrogen fixation, sulphate reduction, membrane stabilisation through cis-trans isomerisation of membrane fatty acids, and the production of biosurfactants and biopolymers in response to the presence of hydrocarbons. A protocol for studying the ability of microbes to control the concentration of hydrocarbons in their aqueous environment is also described, and phenotyping methods to reveal microbes' more general metabolic activities are presented. Several protocols for investigating acid production in connection with oil souring and biocorrosion by microbes in oil well, oil transportation and storage settings are presented. Lastly, protocols for measuring methanogenesis, as an example of microbial hydrocarbon production, are described.Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662570005
Total Pages : 180 pages
Book Rating : 4.5/5 (7 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2018-06-15 with total page 180 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume covers protocols for in-silico approaches to hydrocarbon microbiology, including the selection and use of appropriate statistical tools for experimental design replication, data analysis, and computer-assisted approaches to data storage, management and utilisation. The application of algorithms to analyse the composition and function of microbial communities is presented, as are prediction tools for biodegradation and protein interactions. The basics of a major open-source programming language, Python, are explained. Protocols for calculating reaction kinetics and thermodynamics are presented, and modelling the environmental fate of hydrocarbons during bioremediation is explained. With the exception of molecular biology studies of molecular interactions, the use of statistics is absolutely essential for both experimental design and data analysis in microbiological research, and indeed in the biomedical sciences in general. Moreover, studies of highly varying systems call for the modelling and/or application of theoretical frameworks. Thus, while two protocols in this Volume are specific to hydrocarbon microbiology, the others are generic, and as such will be of use to researchers investigating a broad range of topics in microbiology and the biomedical sciences in general. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662570579
Total Pages : 241 pages
Book Rating : 4.5/5 (75 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2018-07-11 with total page 241 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume addresses the pros and cons of oligonucleotide probes, primers and primer combinations, and importantly considers how to design the best tools for the microbial taxa and/or processes being investigated. Individual chapters focus on the design of primers targeting genes that code for enzymes associated with the following functions: degradation of aromatic, aliphatic and chlorinated hydrocarbons under aerobic and anaerobic conditions, methanogenesis, methane oxidation, and the nitrogen cycle. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662451786
Total Pages : 0 pages
Book Rating : 4.4/5 (517 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2017-06-15 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume describes methods for cultivating hydrocarbon-producing and -consuming microbes, covering compounds in a range of states – gaseous (e.g. methane), liquid (e.g. alkanes of intermediate molecular weight) and solid (e.g. many PAHs and asphaltene). It also examines the cultivation of aerobic and anaerobic hydrocarbon degraders using a range of electron acceptors (e.g. oxygen, nitrate, sulphate, metals, (per)chlorate), and a separate chapter is devoted to explaining the cultivation of methanogens. Special attention is given to: high-throughput cultivation, growing microbes as biofilms, and cultivating fastidious microbes, as well as the preservation of microbial pure cultures and consortia. Accordingly, this Volume will be of value to anyone embarking on the selective enrichment and cultivation of novel microorganisms. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662571156
Total Pages : 362 pages
Book Rating : 4.5/5 (711 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2018-07-11 with total page 362 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume describes methods for investigating microbes in their natural environment and how to obtain representative samples and preserve them for subsequent analyses. Chapters are arranged according to the environments under investigation, which include: oil reservoirs, fracking fluids, aquifers, coal beds, oil sands and their tailing ponds, lakes, rivers, leaves, polar seas and ice, the sea-surface microlayer, mud flats, microbialites, and deep-sea fauna. A variety of downstream analytical procedures are described, including: nucleic-acid extraction and preparation for high-throughput sequencing, fluorescence in-situ hybridisation, and cultivation of aerobic and anaerobic hydrocarbon-degrading microbes. Though most chapters focus on hydrocarbon-rich environments, many of the approaches used are generic, and as such will be of value to researchers embarking on studies of microbes and their processes in the field. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662570838
Total Pages : 328 pages
Book Rating : 4.5/5 (78 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2019-01-23 with total page 328 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher :
ISBN 13 : 9783662491379
Total Pages : 188 pages
Book Rating : 4.4/5 (913 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by . This book was released on 2016 with total page 188 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume provides protocols for the biochemical analysis of hydrocarbon- and lipid-relevant products, cell components and activities of microbes that interact with hydrophobic compounds. They include methods for the extraction, purification and characterisation of surface tension-reducing bioemulsifiers and biosurfactants that increase the surface area and hence bioavailability of hydrophobic substrates. Protocols for the isolation and biochemical analysis of lipids and polyhydroxyalkanoates, food storage products made during nutrient abundance that represent important biotechnological products, are presented. The extraction of membrane lipid rafts, sub-organelles that fulfil important functional roles for the cell membrane, and the isolation and characterisation of membrane phospholipid biomarkers, are also described. The purification and characterisation of integral membrane hydrocarbon-oxidising enzymes are addressed. Lastly, two generic methods for the genetic analysis of catabolic pathways and analysis of ligand binding are presented. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher :
ISBN 13 : 9783662491270
Total Pages : 137 pages
Book Rating : 4.4/5 (912 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by . This book was released on 2016 with total page 137 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents protocols for systems and synthetic biology applications in the field of hydrocarbon and lipid microbiology. It complements another volume that describes generic protocols for wet experimental and computer-based systems and synthetic biology studies. The protocols in this volume demonstrate how to employ systems and synthetic biology approaches in the design of microbes for the production of esters, isoprenoids, hydrophobic polymers, rhamnolipid biosurfactant, and peptide antimicrobial and thioether-stabilised molecules.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662570586
Total Pages : 199 pages
Book Rating : 4.5/5 (75 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2018-06-07 with total page 199 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents generic protocols for wet experimental and computer-based systems and synthetic biology approaches relevant to the field of hydrocarbon and lipid microbiology. It complements a second Volume that describes protocols for systems and synthetic biology applications. The wet experimental tools presented in this Volume include protocols for the standardisation of transcriptional measurements, application of uracil excision-based DNA editing for, inter alia, multi-gene assembly, the use of fluxomics to optimise “reducing power availability”, and the incorporation of non-canonical amino acids into proteins for optimisation of activities. Phenome-ing microbes, using a combination of RNA-seq and bioinformatic algorithms, is presented, as is an illustration, using methylotrophs as an example, of how the different key omics approaches constitute a pipeline for functional analysis, acquisition of a systems overview, and metabolic optimisation. Complementary computational tools that are presented include protocols for probing the genome architecture of regulatory networks, genome-scale metabolic reconstruction, and bioinformatic approaches to guide metabolic engineering. The Volume also includes an overview of how synthetic biology approaches can be used to improve biocontainment. Hydrocarbon and Lipid Microbiology ProtocolsThere are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher :
ISBN 13 : 9783662491348
Total Pages : 174 pages
Book Rating : 4.4/5 (913 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by . This book was released on 2016 with total page 174 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher :
ISBN 13 : 9783662504505
Total Pages : 288 pages
Book Rating : 4.5/5 (45 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by . This book was released on 2016 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: "This Volume presents protocols for investigating the genetic, metabolic and ecological potential and functional analysis of microbial communities. Methods are described for the creation and bioinformatic assessment of metagenomic and metatranscriptomic libraries, and for metaproteomic analyses, which provide important insights into the metabolic potential and interactions of community members. These in turn lead to specific hypotheses concerning the functional contributions of individual populations in the community, which may be investigated by the stable isotope probing approaches described in this Volume, making it possible to identify those community members primarily responsible for particular functions. Methods for the direct extraction of proteins from environmental samples for sequencing and activity tests are presented, providing a broad overview of prevailing metabolic activities and of the types of microbe involved in them. Protocols for the analysis of nutrient flow through microbial communities and for the modelling of dynamic physiological interactions in communities are also provided. Lastly, the book presents a protocol for the quantitative assessment of permissiveness for the transfer of conjugative plasmids, important agents of physiological change and evolution in microbial communities. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes."--Springer.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783662527764
Total Pages : 0 pages
Book Rating : 4.5/5 (277 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J. McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J. McGenity and published by Springer. This book was released on 2016-12-29 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents methods for quantifying microbial populations and characterising microbial communities by extracting and analysing biomarkers such as RNA, DNA and lipids. The chapters cover a wide range of topics, including: cell separation from oil-rich environments, enumeration of hydrocarbon degraders and sulphate reducers using most-probable-number techniques, and quantification by means of real-time PCR. A variety of molecular methods are described for microbial community profiling, such as phospholipid fatty acid analysis, DGGE, T-RFLP and SSCP. One chapter examines high-throughput sequencing, and provides important information on the associated procedures required for thorough data analysis. A further chapter is devoted to the characterisation of protistan communities, while the closing chapter describes multiplex fluorescent antibody microarrays for detecting microbial biomarkers. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662571132
Total Pages : 292 pages
Book Rating : 4.5/5 (711 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2019-01-23 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents applications of hydrocarbon microbiology in the context of environmental pollutant degradation, covering pollutants such as petroleum and related wastes (i.e. oil sludge), biofuels, lipid-rich wastes, chlorinated solvents and BTEX, in several environments (marine, soil, groundwater). The approaches presented range from laboratory experiments and treatment in reactors to field applications. Two chapters highlight innovative approaches to address relevant questions in pollutant degradation, such as low environmental concentrations of pollutants, and the biodegradation of complex pollutant mixtures using biofilms. Rather than presenting the applications in the form of protocols, some of the chapters in this Volume include detailed practical information on the opportunities offered by and limitations of the different approaches, providing valuable information for researchers planning to perform bioremediation experiments. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Hydrocarbon and Lipid Microbiology Protocols

Author : Terry J McGenity
Publisher : Springer
ISBN 13 : 9783662570593
Total Pages : 270 pages
Book Rating : 4.5/5 (75 download)

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Book Synopsis Hydrocarbon and Lipid Microbiology Protocols by : Terry J McGenity

Download or read book Hydrocarbon and Lipid Microbiology Protocols written by Terry J McGenity and published by Springer. This book was released on 2018-10-28 with total page 270 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Volume presents a comprehensive series of generic protocols for the genetic and genomic analysis of prokaryotic isolates. Genetic methods for functional analyses employ the latest cloning vectors, gene fusion methods and transposon mutagenesis systems, as well as systems for introducing protease-cleavage sequences into permissive sites in proteins under investigation. Genomic methods described include protocols for transcriptomics, shotgun proteomics, interactomics, metabolic profiling, and lipidomics. Bioinformatic tools for genome annotation, transcriptome display and the integration of transcriptomic data into genome-scale metabolic reconstructions are described. Protocols for 13C-based metabolic flux determinations and analysis of the hierarchical and metabolic regulation of fluxes through pathways are included. The Volume thus enables investigators to functionally analyse an isolate over the entire cellular range spanning the gene, the genome, the transcript repertoire, the proteome, the interactome, the metabolic network with its nodes and their regulatory hierarchies, and the metabolic fluxes and their physiological controls. Hydrocarbon and Lipid Microbiology Protocols There are tens of thousands of structurally different hydrocarbons, hydrocarbon derivatives and lipids, and a wide array of these molecules are required for cells to function. The global hydrocarbon cycle, which is largely driven by microorganisms, has a major impact on our environment and climate. Microbes are responsible for cleaning up the environmental pollution caused by the exploitation of hydrocarbon reservoirs and will also be pivotal in reducing our reliance on fossil fuels by providing biofuels, plastics and industrial chemicals. Gaining an understanding of the relevant functions of the wide range of microbes that produce, consume and modify hydrocarbons and related compounds will be key to responding to these challenges. This comprehensive collection of current and emerging protocols will facilitate acquisition of this understanding and exploitation of useful activities of such microbes.

Microbial Communities Utilizing Hydrocarbons and Lipids: Members, Metagenomics and Ecophysiology

Author : Terry J. McGenity
Publisher : Springer
ISBN 13 : 9783030147846
Total Pages : 0 pages
Book Rating : 4.1/5 (478 download)

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Book Synopsis Microbial Communities Utilizing Hydrocarbons and Lipids: Members, Metagenomics and Ecophysiology by : Terry J. McGenity

Download or read book Microbial Communities Utilizing Hydrocarbons and Lipids: Members, Metagenomics and Ecophysiology written by Terry J. McGenity and published by Springer. This book was released on 2019-07-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides comprehensive, authoritative discussions about microbial communities in environments that are rich in hydrocarbons, crude oil or lipids. It encompasses natural environments, such as tar sands, oil seeps and reservoirs, as well as habitats where methane is produced. Equally, the book deals with habitats that have been influenced by human activity, including oil-contaminated soils, aquifers, coast and seas. The book opens with a series of chapters considering the contemporary approaches used to investigate microbial communities.