Hplc Columns

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Selection of the HPLC Method in Chemical Analysis

Author : Serban C. Moldoveanu
Publisher : Elsevier
ISBN 13 : 0128037113
Total Pages : 600 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Selection of the HPLC Method in Chemical Analysis by : Serban C. Moldoveanu

Download or read book Selection of the HPLC Method in Chemical Analysis written by Serban C. Moldoveanu and published by Elsevier. This book was released on 2016-11-01 with total page 600 pages. Available in PDF, EPUB and Kindle. Book excerpt: Selection of the HPLC Method in Chemical Analysis serves as a practical guide to users of high-performance liquid chromatography and provides criteria for method selection, development, and validation. High-performance liquid chromatography (HPLC) is the most common analytical technique currently practiced in chemistry. However, the process of finding the appropriate information for a particular analytical project requires significant effort and pre-existent knowledge in the field. Further, sorting through the wealth of published data and literature takes both time and effort away from the critical aspects of HPLC method selection. For the first time, a systematic approach for sorting through the available information and reviewing critically the up-to-date progress in HPLC for selecting a specific analysis is available in a single book. Selection of the HPLC Method in Chemical Analysis is an inclusive go-to reference for HPLC method selection, development, and validation. - Addresses the various aspects of practice and instrumentation needed to obtain reliable HPLC analysis results - Leads researchers to the best choice of an HPLC method from the overabundance of information existent in the field - Provides criteria for HPLC method selection, development, and validation - Authored by world-renowned HPLC experts who have more than 60 years of combined experience in the field

HPLC Columns

Author : Uwe D. Neue
Publisher : Wiley-VCH
ISBN 13 : 9780471190370
Total Pages : 416 pages
Book Rating : 4.1/5 (93 download)

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Book Synopsis HPLC Columns by : Uwe D. Neue

Download or read book HPLC Columns written by Uwe D. Neue and published by Wiley-VCH. This book was released on 1997-07-28 with total page 416 pages. Available in PDF, EPUB and Kindle. Book excerpt: An in-depth guide to HPLC column technology High-performance liquid chromatography and its derivative techniques have become the dominant analytical separation tools in the pharmaceutical, chemical, and food industries; environmental laboratories; and therapeutic drug monitoring. Although the column is the heart of the HPLC instrument and essential to its success, until now, no book has focused on the theory and practice of column technology. HPLC Columns provides thorough, state-of-the-art coverage of HPLC column technology for the practicing technician and academician alike. Along with a comprehensive discussion of the chemical and physical processes of the HPLC column, it includes fundamental principles, separation mechanisms and available technologies, column selection criteria, and special techniques. Special features include: * Comprehensive overview of state-of-the-art HPLC column technology * Explanation of the underlying principles of HPLC columns * Methods for selecting columns * Practical advice on using and applying columns, including examples * Section by M. Zoubair El Fallah on methods development * Special techniques, including preparative chromatography, continuous chromatography, and the simulated moving bed * Troubleshooting section HPLC Columns helps laboratory practitioners make better choices in column selection, methods development, and troubleshooting: it is also an excellent textbook for graduate-level courses and HPLC short courses.

A Practical Handbook of Preparative HPLC

Author : Donald A Wellings
Publisher : Elsevier
ISBN 13 : 0080458858
Total Pages : 193 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis A Practical Handbook of Preparative HPLC by : Donald A Wellings

Download or read book A Practical Handbook of Preparative HPLC written by Donald A Wellings and published by Elsevier. This book was released on 2011-04-18 with total page 193 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is a distillation of twenty years of practical experience of the high pressure liquid chromatography (HPLC) process. Deliberately steering clear of complex theoretical aspects, this book concentrates on the everyday problems associated with the technique, making it perfect for frequent use in the laboratory and for those in the pharmaceutical, agrochemical and biotechnology industries for the analysis and purification of drugs, small molecules, proteins and DNA.This book...•Provides practical, hands-on advice based on years of experience•Will help ensure optimal design, equipment and separation results for your particular task•Presents system layouts from laboratory to process scale•Will help you to devise or improve record-keeping and documentation systems·Provides practical, hands-on advice based on years of experience·Will help ensure optimal design, equipment and separation results for your particular task·Presents system layouts from laboratory to process scale·Will help you to devise or improve record-keeping and documentation systems

High Performance Liquid Chromatography

Author : Omar Al Sayed Omar
Publisher : Walter de Gruyter GmbH & Co KG
ISBN 13 : 3110528959
Total Pages : 501 pages
Book Rating : 4.1/5 (15 download)

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Book Synopsis High Performance Liquid Chromatography by : Omar Al Sayed Omar

Download or read book High Performance Liquid Chromatography written by Omar Al Sayed Omar and published by Walter de Gruyter GmbH & Co KG. This book was released on 2022-02-21 with total page 501 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past decade, modern high-performance liquid chromatography (HPLC) utilization has expanded greatly, especially in the quality control of pharmaceutical products in drug quality control laboratories. This book provides an extensive collection of technical information about HPLC-Columns (physicochemical properties and chromatographic characteristics), from various manufacturers, and helps analysts to decide on the ideal approach for their analysis according to the requirements of drug manufacturers specifi cations and the desired Pharmacopeia. In addition, the authors give practical advice on how to prepare mobile phases, choose a suitable detector, and set up an HPLC analysis. This book is comprehensive for the average professional or technician who plans to work with modern HPLC. This book is useful for most Drug Quality Control Laboratories where modern HPLC is utilized. Following a hands-on approach, the book gives key insights into the pharmaceutical applications of HPLC and the latest requirements of the major regulatory agencies such as ICH, FDA, or USP.

Practical High-Performance Liquid Chromatography

Author : Veronika R. Meyer
Publisher : John Wiley & Sons
ISBN 13 : 9780470093788
Total Pages : 378 pages
Book Rating : 4.0/5 (937 download)

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Book Synopsis Practical High-Performance Liquid Chromatography by : Veronika R. Meyer

Download or read book Practical High-Performance Liquid Chromatography written by Veronika R. Meyer and published by John Wiley & Sons. This book was released on 2004-10 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher Description

Handbook of Pharmaceutical Analysis by HPLC

Author : Satinder Ahuja
Publisher : Elsevier
ISBN 13 : 0080455182
Total Pages : 679 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Handbook of Pharmaceutical Analysis by HPLC by : Satinder Ahuja

Download or read book Handbook of Pharmaceutical Analysis by HPLC written by Satinder Ahuja and published by Elsevier. This book was released on 2005-02-09 with total page 679 pages. Available in PDF, EPUB and Kindle. Book excerpt: High pressure liquid chromatography–frequently called high performance liquid chromatography (HPLC or, LC) is the premier analytical technique in pharmaceutical analysis and is predominantly used in the pharmaceutical industry. Written by selected experts in their respective fields, the Handbook of Pharmaceutical Analysis by HPLC Volume 6, provides a complete yet concise reference guide for utilizing the versatility of HPLC in drug development and quality control. Highlighting novel approaches in HPLC and the latest developments in hyphenated techniques, the book captures the essence of major pharmaceutical applications (assays, stability testing, impurity testing, dissolution testing, cleaning validation, high-throughput screening). A complete reference guide to HPLC Describes best practices in HPLC and offers 'tricks of the trade' in HPLC operation and method development Reviews key HPLC pharmaceutical applications and highlights currents trends in HPLC ancillary techniques, sample preparations, and data handling

High Performance Liquid Chromatography

Author : W.J. Lough
Publisher : CRC Press
ISBN 13 : 9780751400762
Total Pages : 292 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis High Performance Liquid Chromatography by : W.J. Lough

Download or read book High Performance Liquid Chromatography written by W.J. Lough and published by CRC Press. This book was released on 1995-09-30 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: High performance liquid chromatography (HPLC) has long been recognized as one of the most useful and versatile analytical techniques. It has now progressed from being a highly expensive method of analysis to a routine technique with wide applications. Consequently there is a requirement in many chemistry and chemistry-related courses for students to acquire a detailed understanding of the principles and practice of HPLC. Written in a manner suitable for undergraduate students studying analytical chemistry and learning about chromatographic analytical techniques applied to pharmaceutical analysis, biochemistry and related disciplines, High-performance Liquid Chromatography: Fundamental Principles and Practice introduces the fundamentals of HPLC. Loosely structured in three parts, the text begins with a thorough introduction of the subject and then progresses through the essential knowledge of the instrumentation needed for HPLC. The final part covers with the applications of HPLC in real-world situations. Developed by a team of international experts from a wide cross-section of disciplines, the text is relevant to a wide range of courses.

Essentials in Modern HPLC Separations

Author : Serban C. Moldoveanu
Publisher : Elsevier
ISBN 13 : 0323998364
Total Pages : 718 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Essentials in Modern HPLC Separations by : Serban C. Moldoveanu

Download or read book Essentials in Modern HPLC Separations written by Serban C. Moldoveanu and published by Elsevier. This book was released on 2022-06-24 with total page 718 pages. Available in PDF, EPUB and Kindle. Book excerpt: Essentials in Modern HPLC Separations, Second Edition discusses the role of separation in high performance liquid chromatography (HPLC). This new and updated edition systematically presents basic concepts as well as new developments in HPLC. Starting with a description of basic concepts, it provides important guidance for the practical utilization of various HPLC procedures, such as the selection of the HPLC type, proper choice of the chromatographic column, selection of mobile phase and selection of the method of detection, all of which are in correlation with the physico-chemical characteristics of the compounds separated. Every chapter has been carefully reviewed, with several new sections added to bring the book completely up-to-date. Hence, it is a valuable reference for students and professors in chemistry. - Provides a thoroughly updated resource, with an entirely new section on Computer-aided Method Development in HPLC and new subsections on miniaturization and automation in HPLC, chemometric aspects of HPLC, green solvent use in HPLC, and more - Includes insights into the chromatographic process to find the optimum solution for analyzing complex samples - Presents a basis for understanding the utilization of modern HPLC for applications, particularly for the analysis of pharmaceutical, biological, food, beverage and environmental samples

Modern Methods of Pharmaceutical Analysis, Second Edition

Author : Roger E. Schirmer
Publisher : CRC Press
ISBN 13 : 9780849352676
Total Pages : 472 pages
Book Rating : 4.3/5 (526 download)

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Book Synopsis Modern Methods of Pharmaceutical Analysis, Second Edition by : Roger E. Schirmer

Download or read book Modern Methods of Pharmaceutical Analysis, Second Edition written by Roger E. Schirmer and published by CRC Press. This book was released on 1990-12-19 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt:

HPLC and UHPLC for Practicing Scientists

Author : Michael W. Dong
Publisher : John Wiley & Sons
ISBN 13 : 1119313767
Total Pages : 410 pages
Book Rating : 4.1/5 (193 download)

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Book Synopsis HPLC and UHPLC for Practicing Scientists by : Michael W. Dong

Download or read book HPLC and UHPLC for Practicing Scientists written by Michael W. Dong and published by John Wiley & Sons. This book was released on 2019-07-23 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: A concise yet comprehensive reference guide on HPLC/UHPLC that focuses on its fundamentals, latest developments, and best practices in the pharmaceutical and biotechnology industries Written for practitioners by an expert practitioner, this new edition of HPLC and UHPLC for Practicing Scientists adds numerous updates to its coverage of high-performance liquid chromatography, including comprehensive information on UHPLC (ultra-high-pressure liquid chromatography) and the continuing migration of HPLC to UHPLC, the modern standard platform. In addition to introducing readers to HPLC’s fundamentals, applications, and developments, the book describes basic theory and terminology for the novice, and reviews relevant concepts, best practices, and modern trends for the experienced practitioner. HPLC and UHPLC for Practicing Scientists, Second Edition offers three new chapters. One is a standalone chapter on UHPLC, covering concepts, benefits, practices, and potential issues. Another examines liquid chromatography/mass spectrometry (LC/MS). The third reviews at the analysis of recombinant biologics, particularly monoclonal antibodies (mAbs), used as therapeutics. While all chapters are revised in the new edition, five chapters are essentially rewritten (HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects). The book also includes problem and answer sections at the end of each chapter. Overviews fundamentals of HPLC to UHPLC, including theories, columns, and instruments with an abundance of tables, figures, and key references Features brand new chapters on UHPLC, LC/MS, and analysis of recombinant biologics Presents updated information on the best practices in method development, validation, operation, troubleshooting, and maintaining regulatory compliance for both HPLC and UHPLC Contains major revisions to all chapters of the first edition and substantial rewrites of chapters on HPLC columns, instrumentation, pharmaceutical analysis, method development, and regulatory aspects Includes end-of-chapter quizzes as assessment and learning aids Offers a reference guide to graduate students and practicing scientists in pharmaceutical, biotechnology, and other industries Filled with intuitive explanations, case studies, and clear figures, HPLC and UHPLC for Practicing Scientists, Second Edition is an essential resource for practitioners of all levels who need to understand and utilize this versatile analytical technology. It will be a great benefit to every busy laboratory analyst and researcher.

Instrumentation

Author : Debasish Mondal
Publisher : Debasish Mondal
ISBN 13 :
Total Pages : 134 pages
Book Rating : 4./5 ( download)

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Book Synopsis Instrumentation by : Debasish Mondal

Download or read book Instrumentation written by Debasish Mondal and published by Debasish Mondal. This book was released on 2024-05-04 with total page 134 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here's a brief description of each unit: Unit 1: Microscopy Brightfield and darkfield microscopy: Basic techniques for observing biological samples with and without staining. Fluorescence Microscopy: Visualization of fluorescently labeled molecules in biological samples. Phase contrast Microscopy: Enhancing contrast in transparent specimens. Confocal Microscopy: High-resolution imaging technique with optical sectioning capability. Electron Microscopy (Scanning and Transmission Electron Microscopy): High-resolution imaging using electron beams. Micrometry: Measurement of microscopic objects and structures. Unit 2: Chromatography Principles and applications of various chromatographic techniques: Paper chromatography, Thin layer chromatography. Column packing and fraction collection: Preparation and separation of compounds in columns. Gel filtration chromatography: Separation based on molecular size. Ion-exchange chromatography and affinity chromatography: Separation based on charge and specific interactions. Gas-liquid chromatography (GLC) and High-performance liquid chromatography (HPLC): Separation based on different principles. Unit 3: Electrophoresis Principles and applications of various electrophoretic techniques: Polyacrylamide gel electrophoresis, SDS-polyacrylamide gel electrophoresis, 2D gel electrophoresis. Isoelectric focusing: Separation based on differences in isoelectric points. Zymogram preparation: Detection of enzymatic activity in electrophoresis gels. Agarose gel electrophoresis: Separation of nucleic acids based on size. Unit 4: Spectrophotometry Principles of absorption spectroscopy: Measurement of light absorption by biomolecules. UV and visible range analysis: Quantification of biomolecules based on absorption in UV and visible spectra. Colorimetry and turbidometry: Measurement of color changes and turbidity in biochemical assays. Unit 5: Centrifugation Preparative and analytical centrifugation: Separation of particles based on density and size. Fixed angle and swinging bucket rotors: Different configurations for centrifugation. RCF (Relative Centrifugal Force) and sedimentation coefficient: Parameters used to characterize centrifugation. Differential centrifugation and density gradient centrifugation: Techniques for separating particles based on density. Ultracentrifugation: High-speed centrifugation for studying biomolecules and subcellular components.

The HPLC Expert

Author : Stavros Kromidas
Publisher : John Wiley & Sons
ISBN 13 : 3527336818
Total Pages : 378 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis The HPLC Expert by : Stavros Kromidas

Download or read book The HPLC Expert written by Stavros Kromidas and published by John Wiley & Sons. This book was released on 2016-08-08 with total page 378 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid development of HPLC instrumentation and technology opens numerous possibilities - and entails new questions. Which column should I choose to obtain best results, which gradient fits to my analytical problem, what are recent and promising trends in detection techniques, what is state of the art regarding LC-MS coupling? All these questions are answered by experts in ten self-contained chapters. Besides these more hardware-related and technical chapters, further related areas of interest are covered: Comparison of recent chromatographic data systems and integration strategies, smart documentation, efficient information search in internet, and tips for a successful FDA inspection. This practical approach offers in a condensed manner recent trends and hints, and will also display the advanced reader mistakes and errors he was not aware of so far.

Liquid Chromatography

Author : Salvatore Fanali
Publisher : Elsevier
ISBN 13 : 0128093455
Total Pages : 810 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Liquid Chromatography by : Salvatore Fanali

Download or read book Liquid Chromatography written by Salvatore Fanali and published by Elsevier. This book was released on 2017-06-22 with total page 810 pages. Available in PDF, EPUB and Kindle. Book excerpt: Liquid Chromatography: Fundamentals and Instrumentation, Second Edition, is a single source of authoritative information on all aspects of the practice of modern liquid chromatography. It gives those working in both academia and industry the opportunity to learn, refresh, and deepen their understanding of new fundamentals and instrumentation techniques in the field. In the years since the first edition was published, thousands of papers have been released on new achievements in liquid chromatography, including the development of new stationary phases, improvement of instrumentation, development of theory, and new applications in biomedicine, metabolomics, proteomics, foodomics, pharmaceuticals, and more. This second edition addresses these new developments with updated chapters from the most expert researchers in the field. - Emphasizes the integration of chromatographic methods and sample preparation - Explains how liquid chromatography is used in different industrial sectors - Covers the most interesting and valuable applications in different fields, e.g., proteomic, metabolomics, foodomics, pollutants and contaminants, and drug analysis (forensic, toxicological, pharmaceutical, biomedical) - Includes references and tables with commonly used data to facilitate research, practical work, comparison of results, and decision-making

Nutrition and Cancer Prevention

Author : Thomas Moon
Publisher : CRC Press
ISBN 13 : 9780824779931
Total Pages : 610 pages
Book Rating : 4.7/5 (799 download)

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Book Synopsis Nutrition and Cancer Prevention by : Thomas Moon

Download or read book Nutrition and Cancer Prevention written by Thomas Moon and published by CRC Press. This book was released on 1988-10-21 with total page 610 pages. Available in PDF, EPUB and Kindle. Book excerpt: Scientific advances have led to the recognition that many chronic diseases such as cancer may be preventable. In this volume, 36 contributions test cancer prevention hypotheses, attempt to interpret their results, and provide a guide to the background, rationale, and selection of cancer prevention a

Analytical Chemistry-A Qualitative and Quantitative Approach

Author : Deepak Chowrasia
Publisher : Deepak Chowrasia
ISBN 13 : 9352791150
Total Pages : 210 pages
Book Rating : 4.3/5 (527 download)

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Book Synopsis Analytical Chemistry-A Qualitative and Quantitative Approach by : Deepak Chowrasia

Download or read book Analytical Chemistry-A Qualitative and Quantitative Approach written by Deepak Chowrasia and published by Deepak Chowrasia. This book was released on with total page 210 pages. Available in PDF, EPUB and Kindle. Book excerpt: Book envelops various analytical procedures including their principle and application in chemical and drug analysis.

Introduction to Modern Liquid Chromatography

Author : Lloyd R. Snyder
Publisher : John Wiley & Sons
ISBN 13 : 1118210395
Total Pages : 831 pages
Book Rating : 4.1/5 (182 download)

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Book Synopsis Introduction to Modern Liquid Chromatography by : Lloyd R. Snyder

Download or read book Introduction to Modern Liquid Chromatography written by Lloyd R. Snyder and published by John Wiley & Sons. This book was released on 2011-09-20 with total page 831 pages. Available in PDF, EPUB and Kindle. Book excerpt: The latest edition of the authoritative reference to HPLC High-performance liquid chromatography (HPLC) is today the leading technique for chemical analysis and related applications, with an ability to separate, analyze, and/or purify virtually any sample. Snyder and Kirkland's Introduction to Modern Liquid Chromatography has long represented the premier reference to HPLC. This Third Edition, with John Dolan as added coauthor, addresses important improvements in columns and equipment, as well as major advances in our understanding of HPLC separation, our ability to solve problems that were troublesome in the past, and the application of HPLC for new kinds of samples. This carefully considered Third Edition maintains the strengths of the previous edition while significantly modifying its organization in light of recent research and experience. The text begins by introducing the reader to HPLC, its use in relation to other modern separation techniques, and its history, then leads into such specific topics as: The basis of HPLC separation and the general effects of different experimental conditions Equipment and detection The column—the "heart" of the HPLC system Reversed-phase separation, normal-phase chromatography, gradient elution, two-dimensional separation, and other techniques Computer simulation, qualitative and quantitative analysis, and method validation and quality control The separation of large molecules, including both biological and synthetic polymers Chiral separations, preparative separations, and sample preparation Systematic development of HPLC separations—new to this edition Troubleshooting tricks, techniques, and case studies for both equipment and chromatograms Designed to fulfill the needs of the full range of HPLC users, from novices to experts, Introduction to Modern Liquid Chromatography, Third Edition offers the most up-to-date, comprehensive, and accessible survey of HPLC methods and applications available.

Liquid Chromatography

Author : K.K. Unger
Publisher : Elsevier Inc. Chapters
ISBN 13 : 0128071303
Total Pages : 69 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Liquid Chromatography by : K.K. Unger

Download or read book Liquid Chromatography written by K.K. Unger and published by Elsevier Inc. Chapters. This book was released on 2013-01-08 with total page 69 pages. Available in PDF, EPUB and Kindle. Book excerpt: This chapter deals with the most important part of the LC system: the column enabling the efficient and fast resolution of complex mixtures. It is divided into seven sections under the overall aspects of manufacture, operation, and evaluation of analytical columns for the user. The first three sections highlight the column design and hardware, followed by a thorough treatment of the properties of microparticulate silica adsorbents as packing material, stationary phases performed by surface functionalization, and the column filling process. Then, the implementation of the column into the LC system is discussed, leading into chromatographic column testing as a C18 bonded reversed silica column and its maintenance. Section 3.7 is devoted to today’s column market, focusing on the three main types of silica columns: fully porous microparticulate silica columns , columns with core–shell silica particles, and monolithic silica columns. Within a period of 40 years, analytical HPLC has generated high-resolution, robust, and rugged reversed-phase silica columns, which enable efficient, highly sensitive, and fast separations of low-molecular-weight substances based on validated methods.