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Author : Cindy Maria Quezada
Publisher :
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.:/5 (437 download)
Download or read book Histidine Phosphorylation in Bacterial Chemotaxis written by Cindy Maria Quezada and published by . This book was released on 2003 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Masayori Inouye
Publisher : Elsevier
ISBN 13 : 0080534015
Total Pages : 539 pages
Book Rating : 4.0/5 (85 download)
Download or read book Histidine Kinases in Signal Transduction written by Masayori Inouye and published by Elsevier. This book was released on 2002-11-13 with total page 539 pages. Available in PDF, EPUB and Kindle. Book excerpt: Living cells are constantly sensing environmental changes, and their abilities to sense these changes and adapt to them are essential for their survival. In bacteria, histidine kinases are the major sensors for these environmental stresses, enabling cells to adapt to new growth conditions. Written by leading experts in the field, this book provides an up-to-date and comprehensive review on the structure and function of histidine kinases. It also provides extensive information on the physiological roles of histidine kinases in bacteria and eukaryotes. An an essential reference for cell biologists, microbiologists, molecular biologists, and biochemists interested in signal transduction. Experimental biologists and pharmacologists studying signal transduction systems in living organisms will also find it a valuable research tool. - The first comprehensive book on the roles of histidine kinases in cells - 23 in-depth chapters written by leading experts in the field - Describes the most recent advances in the field of signal transduction
Author : Wenlin Pan
Publisher :
ISBN 13 :
Total Pages : 87 pages
Book Rating : 4.:/5 (12 download)
Download or read book Kinetic Study of Histidine Kinase CheA in Bacterial Chemotaxis written by Wenlin Pan and published by . This book was released on 2017 with total page 87 pages. Available in PDF, EPUB and Kindle. Book excerpt: Histidine kinase CheA is central to signaling in bacterial chemotaxis. This kinase is responsible for the phosphorylation of two response regulators, CheY and CheB. CheY controls flagellar rotation and thus motility. CheB is crucial for sensory adaptation. CheA is dramatically activated, up to 1000-fold, and put under the control of chemoreceptors by formation of the signaling complex. As measured by phosphorylation of CheY, this control modulates the activity of CheA in a range as wide as two orders of magnitude. This change in the activity of CheA is the essence of chemotactic signaling. However, the enzymatic properties altered by kinase incorporation into signaling complexes, chemoreceptor ligand binding or receptor adaptational modification are largely undefined. This dissertation describes the kinetic analysis of kinase CheA. Data are fit to the Michaelis-Menten equation, from which important parameters KM and kcat are obtained. Based on these parameters for CheA at different signaling conditions, important observations and conclusions are made to contribute to better understanding of the control of the activity of kinase CheA, thus the bacterial chemotaxis signaling system.
Author : Claire E. Eyers
Publisher : Humana
ISBN 13 : 9781493998869
Total Pages : 256 pages
Book Rating : 4.9/5 (988 download)
Download or read book Histidine Phosphorylation written by Claire E. Eyers and published by Humana. This book was released on 2020-11-10 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details the current understanding of roles and regulation on histidine phosphorylation, describing methods for the characterization of protein phosphorylation on histidine. Chapters guide readers through in vitro systems, cell-based systems, comprehensive background review articles on histidine kinases and phosphatases. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Histidine Phosphorylation: Methods and Protocols aims to ensure successful results in the further study of this rapidly growing field.
Author : Sang-Youn Richard Park
Publisher :
ISBN 13 :
Total Pages : 400 pages
Book Rating : 4.E/5 ( download)
Download or read book Bacterial Chemotaxis as a Model System in Signal Transduction written by Sang-Youn Richard Park and published by . This book was released on 2005 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Anna Rusch Greenswag
Publisher :
ISBN 13 :
Total Pages : 240 pages
Book Rating : 4.:/5 (919 download)
Download or read book Structure and Enzymology of the Chemotaxis Histidine Kinase, CheA written by Anna Rusch Greenswag and published by . This book was released on 2015 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bacterial chemotaxis enables changes in motility via response to the surrounding chemical environment and is noted for its high signal gain, range, and sensitivity. The efficacy of the bacterial chemotaxis signaling pathway is highly dependent on the propagation of the extracellular chemical signal through a hexagonal array comprised of: methyl-accepting chemotaxis protein receptors, histidine kinase CheA, and coupling protein CheW. CheA is the principal enzyme in the chemotaxis pathway and is composed of five domains (P1-5). Initiation of the phospho-relay by CheA ends in rotational switching of the flagellar rotor. CheA only achieves a broad range of autophosphorylation activity when associated with chemoreceptors. This dissertation focuses on the structural and biochemical changes during the CheA autophosphorylation event. The propensity of Thermotoga maritima CheA to naturally undergo trans autophosphorylation was elucidated and strategic mutations enabled generation of disulfide-locked CheA variants to further probe protein dynamics. Employing small-angle x-ray scattering (SAXS), the resting state of CheA was determined to be globular. Where upon nucleotide addition the protein transitioned to a dynamic state as a result of the movement of P1 and P4 domains to facilitate transfer of the [gamma]-phosphate. Coupling crystallographic and biochemical data, a model was generated of CheA that is able to account for variances in enzymatic activity, incorporating key structural features to the functional response of signal transduction. To further the understanding the influence the receptors impart to CheA, chemoreceptor cytoplasmic kinase-control modules based on the E. coli aspartate receptor, Tar, were covalently fused into a dimer and trimerized by a foldon domain (TarFO). SAXS, multiangle light scattering, and pulsed-dipolar electron paramagnetic resonance spectroscopy of spinlabeled proteins indicate that the TarFO is soluble, monodisperse, and assembles into homogenous trimers wherein the protein interaction regions closely associate at the opposite ends of the foldon domain. The TarFO activates CheA autophosphorylation to the same degree as membrane integrated receptors and stabilizes a planar conformation of the kinase consistent with current array models for the assembly state of the ternary complex. Overall, these studies illuminate a planar CheA active structure and provide a more in depth investigation of the CheA autophosphorylation event.
Author : Sebastian Himmel
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (819 download)
Download or read book Histidine Phosphorylation in Proteins written by Sebastian Himmel and published by . This book was released on 2011 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Roy Gross
Publisher : Caister Academic Press Limited
ISBN 13 : 9781908230089
Total Pages : 0 pages
Book Rating : 4.2/5 (3 download)
Download or read book Two-component Systems in Bacteria written by Roy Gross and published by Caister Academic Press Limited. This book was released on 2012 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Current information on two-component systems in bacteria including structure-function analysis, sensing mechanisms, atypical two-component systems, stress responses, developmental processes, virulence and symbiosis.
Author : Reinhard Krämer
Publisher : John Wiley & Sons
ISBN 13 : 3527629246
Total Pages : 513 pages
Book Rating : 4.5/5 (276 download)
Download or read book Bacterial Signaling written by Reinhard Krämer and published by John Wiley & Sons. This book was released on 2009-12-09 with total page 513 pages. Available in PDF, EPUB and Kindle. Book excerpt: Providing a comprehensive insight into cellular signaling processes in bacteria with a special focus on biotechnological implications, this is the first book to cover intercellular as well as intracellular signaling and its relevance for biofilm formation, host pathogen interactions, symbiotic relationships, and photo- and chemotaxis. In addition, it deals in detail with principal bacterial signaling mechanisms -- making this a valuable resource for all advanced students in microbiology. Dr. Krämer is a world-renowned expert in intracellular signaling and its implications for biotechnology processes, while Dr. Jung is an expert on intercellular signaling and its relevance for biomedicine and agriculture.
Author :
Publisher : Elsevier
ISBN 13 : 0080548717
Total Pages : 588 pages
Book Rating : 4.0/5 (85 download)
Download or read book Two-Component Signaling Systems, Part A written by and published by Elsevier. This book was released on 2011-09-21 with total page 588 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multicellular organisms must be able to adapt to cellular events to accommodate prevailing conditions. Sensory-response circuits operate by making use of a phosphorylation control mechanism known as the "two-component system." Sections include: Computational Analyses of Sequences and Sequence Alignments Biochemical and Genetic Assays of Individual Components of Signaling Systems Physiological Assays and Readouts - Presents detailed protocols - Includes troubleshooting tips
Author : Ron Milo
Publisher : Garland Science
ISBN 13 : 1317230698
Total Pages : 400 pages
Book Rating : 4.3/5 (172 download)
Download or read book Cell Biology by the Numbers written by Ron Milo and published by Garland Science. This book was released on 2015-12-07 with total page 400 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Top 25 CHOICE 2016 Title, and recipient of the CHOICE Outstanding Academic Title (OAT) Award. How much energy is released in ATP hydrolysis? How many mRNAs are in a cell? How genetically similar are two random people? What is faster, transcription or translation?Cell Biology by the Numbers explores these questions and dozens of others provid
Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ashraf Brik
Publisher : John Wiley & Sons
ISBN 13 : 3527346600
Total Pages : 626 pages
Book Rating : 4.5/5 (273 download)
Download or read book Total Chemical Synthesis of Proteins written by Ashraf Brik and published by John Wiley & Sons. This book was released on 2021-06-08 with total page 626 pages. Available in PDF, EPUB and Kindle. Book excerpt: How to synthesize native and modified proteins in the test tube With contributions from a panel of experts representing a range of disciplines, Total Chemical Synthesis of Proteins presents a carefully curated collection of synthetic approaches and strategies for the total synthesis of native and modified proteins. Comprehensive in scope, this important reference explores the three main chemoselective ligation methods for assembling unprotected peptide segments, including native chemical ligation (NCL). It includes information on synthetic strategies for the complex polypeptides that constitute glycoproteins, sulfoproteins, and membrane proteins, as well as their characterization. In addition, important areas of application for total protein synthesis are detailed, such as protein crystallography, protein engineering, and biomedical research. The authors also discuss the synthetic challenges that remain to be addressed. This unmatched resource: Contains valuable insights from the pioneers in the field of chemical protein synthesis Presents proven synthetic approaches for a range of protein families Explores key applications of precisely controlled protein synthesis, including novel diagnostics and therapeutics Written for organic chemists, biochemists, biotechnologists, and molecular biologists, Total Chemical Synthesis of Proteins provides key knowledge for everyone venturing into the burgeoning field of protein design and synthetic biology.
Author : Günter Hauska
Publisher : Springer Science & Business Media
ISBN 13 : 3642759696
Total Pages : 190 pages
Book Rating : 4.6/5 (427 download)
Download or read book The Molecular Basis of Bacterial Metabolism written by Günter Hauska and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: The present volume contains 17 lectures of the 41 st Mosbach Colloquium of the Gesellschaft fiir Biologische Chemie, held from April 5-7, 1990 on the topic "The Molecular Basis of Bacterial Metabolism". From the beginning it was not the intention of the organizers to present a comprehensive account, but rather to select new, exciting progress on sometimes exotic reactions of specifically bacterial, mainly anaerobic metabolism. Members of our society had contributed to this progress to an extent that greatly stimulated the scientific exchange with international colleagues during the days in Mosbach. The editors hope that this stimulation will be conveyed to the readers of the articles, which reach from the biochemistry of methanogenesis, via anaerobic radical reactions, metal biochemistry in hydrogen and nitrogen metabolism, conversions of light - and redox energy, to the regulation of metabolic adaptation, and the attempts to bioengineer novel pathways for the degradation of xenobiotica. We believe that the book represents a highly progressive field of over lapping disciplines, comprising microbiology and molecular genetics, chemistry of biomimetic interest, and biophysics, and that it gives insight into the impact modern technologies have on microbiological research today. The colloquium was generously supported by the Deutsche Forschungsgemeinschaft, the Paul-Martini-Stiftung, and the Fonds fiir Biologische Chemie. A. Trebst, G. Schafer, and D. Oesterhelt were a great help in preparing the program and we wish to thank them for their advice.
Author : Society for General Microbiology. Symposium
Publisher : Cambridge University Press
ISBN 13 : 9780521403139
Total Pages : 424 pages
Book Rating : 4.4/5 (31 download)
Download or read book Biology of the Chemotactic Response written by Society for General Microbiology. Symposium and published by Cambridge University Press. This book was released on 1990 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: The tendency of a living organism to move to a more favourable environment is a natural but complex reaction, involving the integration of sometimes conflicting environmental stimuli as well as a coordinated mechanical response. The response of motile, single cell organisms to environmental stimuli provides a useful model for understanding first of all how the environment is monitored and sensed, and secondly how this information is processed to result in an integrated and coordinated response. The volume looks at a large number of well-studied examples of the chemotactic response, in prokaryotes and eukaryotes, and casts new light on how cells process information and react to their environment. This fundamental response is of great importance in understanding one of the characteristic features of living organisms.
Author : Vana Smith
Publisher : CRC Press
ISBN 13 : 9780849388057
Total Pages : 554 pages
Book Rating : 4.3/5 (88 download)
Download or read book Stimulus Response Coupling written by Vana Smith and published by CRC Press. This book was released on 1990-10-24 with total page 554 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume examines the role of intracellular calcium in the transmission of external chemical, physical and electrical stimuli to the interior of the cell and the role of calcium in the physiological and metabolic effects of such stimuli.
Author : James A. Hoch
Publisher : Amer Society for Microbiology
ISBN 13 : 9781555810894
Total Pages : 488 pages
Book Rating : 4.8/5 (18 download)
Download or read book Two-component Signal Transduction written by James A. Hoch and published by Amer Society for Microbiology. This book was released on 1995 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: The human enteroviruses, particularly the polio viruses, have had a significant role in the history of medicine and microbiology; and continue to cause clinical problems, as well as provide targets for molecular investigation. This book offers a link between the basic science and clinical medicine.
