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Handbook Of Biomedical Nonlinear Optical Microscopy
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Book Synopsis Handbook of Biomedical Nonlinear Optical Microscopy by : Barry R. Masters
Download or read book Handbook of Biomedical Nonlinear Optical Microscopy written by Barry R. Masters and published by Oxford University Press. This book was released on 2008-05-19 with total page 895 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Biomedical Nonlinear Optical Microscopy provides comprehensive treatment of the theories, techniques, and biomedical applications of nonlinear optics and microscopy for cell biologists, life scientists, biomedical engineers, and clinicians. The chapters are separated into basic and advanced sections, and provide both textual and graphical illustrations of all key concepts. The more basic sections are aimed at life scientists without advanced training in physics and mathematics, and tutorials are provided for the more challenging sections. The first part of the Handbook introduces the historical context of nonlinear microscopy. The second part presents the nonlinear optical theory of two- and multiphoton excited fluorescence (TPE, MPE) spectroscopy, second and third harmonic generation (SHG, THG) spectroscopy, and coherent anti-Stokes Raman spectroscopy (CARS). The third part introduces modern microscopic and spectroscopic instrumentation and techniques that are based on nonlinear optics. The fourth part provides key applications of nonlinear microscopy to the biomedical area: neurobiology, immunology, tumor biology, developmental biology, dermatology, and cellular metabolism. There are also chapters on nonlinear molecular probes, cellular damage, and nanoprocessing.
Book Synopsis Handbook of Biomedical Optics by : David A. Boas
Download or read book Handbook of Biomedical Optics written by David A. Boas and published by CRC Press. This book was released on 2016-04-19 with total page 816 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biomedical optics holds tremendous promise to deliver effective, safe, non- or minimally invasive diagnostics and targeted, customizable therapeutics. Handbook of Biomedical Optics provides an in-depth treatment of the field, including coverage of applications for biomedical research, diagnosis, and therapy. It introduces the theory and fundamental
Book Synopsis The bh TCSPC Handbook by : Dr. Wolfgang Becker
Download or read book The bh TCSPC Handbook written by Dr. Wolfgang Becker and published by Becker & Hickl GmbH. This book was released on 2021-09-01 with total page 995 pages. Available in PDF, EPUB and Kindle. Book excerpt: Time-Correlated Single Photon Counting Modules SPC-130EMN, SPC-130EMNX, SPC-130IN, SPC-130INX, SPC-150N, SPC-150NX, SPC-150NXX, SPC-160, SPC-160PCIE, SPC-180N, SPC-180NX, SPC-180NXX Detectors, Lasers and Peripheral Devices Simple-Tau Systems Technical Principles TCSPC Applications FLIM Systems Applications in Life Sciences Clinical FLIM Applications SPCM Software SPCImage NG Data Analysis Software Time-correlated single photon counting (TCSPC) is an amazingly sensitive technique for recording low-level light signals with picosecond resolution and extremely high precision.TCSPC originates from the measurement of excited nuclear states and has been used since the late 60s [775, 1250]. For many years TCSPC was used primarily to record fluorescence decay curves of organic dyes in solution. Due to the low intensity and low repetition rate of the light sources and the limited speed of the electronics of the 70s and 80s the acquisition times were extremely long. More important, classic TCSPC was intrinsically one-dimensional, i.e. limited to the recording of the waveform of a periodic light signal. Light sources ceased to be a limitation when the first mode-locked Argon lasers and synchronously pumped dye lasers were introduced. For the recording electronics, the situation changed with the introduction of the SPC-300 modules of Becker & Hickl in 1993. Due to a new analog-to-digital conversion principle these modules could be used at photon count rates almost 100 times higher than the classic TCSPC devices. Moreover, the modules were able to record the photons of a large number of detectors simultaneously. They were thus able to record a photon distribution not only versus the time in a fluorescence decay but also versus aspatial coordinate or the wavelength of the photons. Multi-dimensional TCSPC was born. Within a few years, more dimensions were added to multidimensional TCSPC. Fast sequential recording was introduced with the SPC-430 in 1995, fast scanning with the SPC-535 in 1997. Time-tag recording was introduced with the SPC-431 in 1996; multi-module TCSPC systems followed in 1999. Since then, the Becker & Hickl TCSPC systems became bigger, faster and more flexible. Recent TCSPC modules, like the SPC-150NX or the SPC-180, can be configured for sequential recording, imaging, or time-tag recording by a simple software command. Multi-module systems, like the SPC-134EM and SPC-154, can be used for scanning at unprecedented count rates and acquisition speeds. Nevertheless, TCSPC still has the reputation to be an extremely sluggish technique unable to record any fast changes in the fluorescence or scattering behaviour of a sample. The multidimensional features of modern TCSPC are not commonly understood. Thus, many users do not make efficient use of their SPC modules. However, if appropriately used, multidimensional TCSPC techniques not only deliver superior results but also solve highly sophisticated measurement problems. This handbook is an attempt to help existing and potential users understand and make use of the advanced features of modern TCSPC. After an introduction into the bh TCSPC devices and associated detector, laser, and experiment control modules the principles of advanced TCSPC techniques are described. These include multidetector TCSPC, multiplexed TCSPC, sequential recording techniques, scanning techniques, parameter-tag recording, and multi-module TCSPC techniques. The next chapter describes the architecture of the bh SPC modules. A chapter about detectors gives a review of detector principles and of the parameters used to characterise detectors. It describes a number of detectors commonly used for TCSPC and gives advice about obtaining best performance from them. The implementation of bh SPC devices is described in the next part of the handbook. It includes principles and wiring diagrams for typical experiments, guidelines for first system setup, and advice for system optimisation. It describes dead-time, counting loss, and pile-up effects, detector effects, and effects related to the optical system. The next chapter of the handbook is dedicated to TCSPC applications. The first part of this chapter describes the measurement of fluorescence and anisotropy decay curves, multispectral lifetime experiments, recording of transient fluorescence lifetime phenomena, and measurements of phosphorescence decay curves. The second part of the chapter is dedicated to time-resolved laser scanning microscopy. It contains sections on a wide variety of fluorescence-lifetime imaging (FLIM) experiments and procedures, such as FLIM with various excitation principles, excitation sources, and detection principles, high-speed and time-series FLIM, Z-stack FLIM, simultaneous fluorescence and phosphorescence lifetime imaging (FLIM/PLIM), fluorescence lifetime-transient scanning (FLITS), and FLIM with special microscope configurations. A third part contains FLIM background knowledge: Signal-to-noise ratio, acquisition time, the effect of counting loss and pile-up, photobleaching, and fluorescence depolarisation on the recorded data. The book contains a large chapter on TCSPC applications, most of them in Biology. It contains sections on FLIM of molecular environment parameters in tissue, FLIM-based FRET measurements in cells, autofluorescence FLIM of biological tissue, plant physiology, and clinical FLIM applications. A section about diffuse optical tomography (DOT) by NIRS techniques includes breast imaging, static and functional brain imaging, perfusion measurement in the human brain, diffuse tissue spectroscopy, and small-animal imaging. Picosecond photon correlation, fluorescence correlation spectroscopy, burst-integrated fluorescence lifetime techniques, and photon counting histogram techniques are reviewed in the next sections. The last part of the application chapter gives an review of non-biological TCSPC applications like positron lifetime measurement, measurement of barrier discharges, remote sensing, metrological applications, and characterisation of detectors. The application chapter also includes practical hints about optical systems, detectors, and other technical aspects of the applications described. Another large chapter describes the SPCM operating software of the bh SPC modules. It describes the various user interface configurations, operation modes, the system and control parameters, the handling and display of the multidimensional data recorded by the modules, and the associated data file structure. The TCSPC Handbook also contains a chapter on the SPCImage NG fluorescence decay and FLIM data analysis software. It describes the general principles of fluorescence decay analysis, the calculation of fluorescence decay parameters and lifetime images by various decay models, pseudo-global analysis, multi-wavelength FLIM analysis, batch-processing of FLIM series, and analysis of PLIM data. The handbook ends with a list of more than 1200 references related to TCSPC, most of them being applications of the bh SPC devices.
Book Synopsis Handbook of Photonics for Biomedical Science by : Valery V. Tuchin
Download or read book Handbook of Photonics for Biomedical Science written by Valery V. Tuchin and published by CRC Press. This book was released on 2010-05-18 with total page 863 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Photonics for Biomedical Science analyzes achievements, new trends, and perspectives of photonics in its application to biomedicine. With contributions from world-renowned experts in the field, the handbook describes advanced biophotonics methods and techniques intensively developed in recent years.Addressing the latest problems in
Book Synopsis Superresolution Optical Microscopy by : Barry R. Masters
Download or read book Superresolution Optical Microscopy written by Barry R. Masters and published by Springer Nature. This book was released on 2020-03-21 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent summary of techniques for enhancing the resolution and image contrast provided by far-field optical microscopes. It takes a critical look at the body of knowledge that comprises optical microscopy, compares and contrasts the various instruments, provides a clear discussion of the physical principles that underpin these techniques, and describes advances in science and medicine for which superresolution microscopes are required and are making major contributions. The text fills significant gaps that exist in other works on superresolution imaging, firstly by placing a new emphasis on the specimen, a critical component of the microscope setup, giving equal importance to the enhancement of both resolution and contrast. Secondly, it covers several topics not typically discussed in depth, such as Bessel and Airy beams, the physics of the spiral phase plate, vortex beams and singular optics, photoactivated localization microscopy (PALM), stochastic optical reconstruction microscopy (STORM), structured illumination microscopy (SIM), and light-sheet fluorescence microscopy (LSFM). Several variants of these techniques are critically discussed. Noise, optical aberrations, specimen damage, and artifacts in microscopy are also covered. The importance of validation of superresolution images with electron microscopy is stressed. Additionally, the book includes translations and discussion of seminal papers by Abbe and Helmholtz that proved to be pedagogically relevant as well as historically significant. This book is written for students, researchers, and engineers in the life sciences, medicine, biological engineering, and materials science who plan to work with or already are working with superresolution light microscopes. The volume can serve as a reference for these areas while a selected set of individual chapters can be used as a textbook for a one-semester undergraduate or first-year graduate course on superresolution microscopy. Moreover, the text provides a captivating account of curiosity, skepticism, risk-taking, innovation, and creativity in science and technology. Good scientific practice is emphasized throughout, and the author’s lecture slides on responsible conduct of research are included as an online resource which will be of interest to students, course instructors, and scientists alike.
Book Synopsis Advances in Brain Imaging Techniques by : Nirmal Mazumder
Download or read book Advances in Brain Imaging Techniques written by Nirmal Mazumder and published by Springer Nature. This book was released on 2022-05-19 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: The book reviews the recent developments in brain imaging and their technological advancements to understand molecular mechanisms associated with neurological disorders and basic behaviors in humans and rodents at the structural, molecular, and functional levels. It discusses the usefulness of advanced optical microscopy techniques, including optical coherence tomography (OCT), miniscope, multiphoton fluorescence (2PF & 3PF), adaptive optics, harmonic generation, and Raman microscopy for understanding pathomechanism of brain disorders and pathological and physiological changes associated with neurodegenerative diseases. Also, the book presents conventional imaging modalities, including Magnetic Resonance Imaging (MRI), for delineating underlying mechanisms and precise early diagnosis of neurological disorders. This book is a useful resource for neuroscientists and researchers working in biomedical engineering and optics.
Book Synopsis Miniaturized Analytical Devices by : Suresh Kumar Kailasa
Download or read book Miniaturized Analytical Devices written by Suresh Kumar Kailasa and published by John Wiley & Sons. This book was released on 2021-12-17 with total page 324 pages. Available in PDF, EPUB and Kindle. Book excerpt: Miniaturized Analytical Devices An in-depth overview of integrating functionalized nanomaterials with mass spectrometry, spectroscopy, electrophoresis, and other important analytical techniques Miniaturized Analytical Devices: Materials and Technology is an up-to-date resource exploring the analytical applications of miniaturized technology in areas such as clinical microbiology, pharmaceuticals, agriculture, and environmental analysis. The book covers the integration of functional nanomaterials in mass spectrometry, microscopy, electrophoresis, and more—providing the state-of-the-art information required for successfully implementing a range of chemical analysis techniques on microchips. Featuring contributions from a panel of international experts in the field, the book begins with an introduction to selected miniaturized devices, nanomaterials, and analytical methods. Subsequent sections describe functionalized nanomaterials (FNMs) for miniaturized devices and discuss techniques such as miniaturized mass spectrometry for bioassays and miniaturized microscopy for cell imaging. The book concludes by exploring a variety of applications of miniaturized devices in areas including metal analysis, bioimaging, DNA separation and analysis, molecular biology, and more. This timely volume: Surveys the current state of the field and provides a starting point for developing faster, more reliable, and more selective analytical devices Focuses on the practical applications of miniaturized analytical devices in materials science, clinical microbiology, the pharmaceutical industry, and environmental analysis Covers a wide range of materials and analytical techniques such as microvolume UV-VIS spectroscopy, microchip and capillary electrophoresis, and matrix assisted laser desorption ionization-mass spectrometry (MALDI-MS) analysis Discusses the role of miniaturized analytical devices in securing a green and sustainable future Miniaturized Analytical Devices: Materials and Technology is essential reading for analytical chemists, analytical laboratories, materials scientists, biologists, life scientists, and advanced students in related fields.
Book Synopsis Fluorescence Lifetime Spectroscopy and Imaging by : Laura Marcu
Download or read book Fluorescence Lifetime Spectroscopy and Imaging written by Laura Marcu and published by CRC Press. This book was released on 2014-07-17 with total page 574 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the past two decades, there has been an increasing appreciation of the significant value that lifetime-based techniques can add to biomedical studies and applications of fluorescence. Bringing together perspectives of different research communities, Fluorescence Lifetime Spectroscopy and Imaging: Principles and Applications in Biomedical Diagnostics explores the remarkable advances in time-resolved fluorescence techniques and their role in a wide range of biological and clinical applications. Broadly accessible, the book captures the state-of-the-art of fluorescence lifetime metrology and imaging and provides current perspectives on their applications to biomedical studies of intact tissues and medical diagnosis. The text introduces these techniques within the wider context of fluorescence spectroscopy and describes basic principles underlying current instrumentation for fluorescence lifetime imaging and metrology (FLIM). It also covers the wide range of methods, including single channel (point) spectroscopy, fluorescence lifetime imaging microscopy, and single- and multi-photon excitation. Edited by pioneers in this field, with contributions from leading experts, the book includes an overview of complementary techniques that help researchers beginning FLIM research. It offers a comprehensive treatment of fundamental principles, instrumentation, analytical methods, and applications. It also provides an overview of the label-free contrast available from lifetime measurements of tissue autofluorescence and the prospects for exploiting this for clinical applications and biomedical research including drug discovery.
Book Synopsis Advanced Time-Correlated Single Photon Counting Applications by : Wolfgang Becker
Download or read book Advanced Time-Correlated Single Photon Counting Applications written by Wolfgang Becker and published by Springer. This book was released on 2015-04-13 with total page 642 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is an attempt to bridge the gap between the instrumental principles of multi-dimensional time-correlated single photon counting (TCSPC) and typical applications of the technique. Written by an originator of the technique and by sucessful users, it covers the basic principles of the technique, its interaction with optical imaging methods and its application to a wide range of experimental tasks in life sciences and clinical research. The book is recommended for all users of time-resolved detection techniques in biology, bio-chemistry, spectroscopy of live systems, live cell microscopy, clinical imaging, spectroscopy of single molecules, and other applications that require the detection of low-level light signals at single-photon sensitivity and picosecond time resolution.
Book Synopsis Multi-dimensional Imaging by : Bahram Javidi
Download or read book Multi-dimensional Imaging written by Bahram Javidi and published by John Wiley & Sons. This book was released on 2014-03-26 with total page 464 pages. Available in PDF, EPUB and Kindle. Book excerpt: Provides a broad overview of advanced multidimensional imaging systems with contributions from leading researchers in the field Multi-dimensional Imaging takes the reader from the introductory concepts through to the latest applications of these techniques. Split into 3 parts covering 3D image capture, processing, visualization and display, using 1) a Multi-View Approach and 2.) a Holographic Approach, followed by a 3rd part addressing other 3D systems approaches, applications and signal processing for advanced 3D imaging. This book describes recent developments, as well as the prospects and challenges in advances in imaging sciences and engineering such as 3D image sensing, 3D holographic imaging, imaging applications for bio-photonics and 3D image recognition. Advanced imaging systems incorporate knowledge from various fields. It is a complex technology that combines physics, optics, signal processing, and image capture techniques. Provides a broad overview of advanced multidimensional imaging systems with contributions from leading researchers in the field. Integrates the background, introductory material with new advances in 3D imaging and applications. Covers the most recent technologies such as high speed digital holography, compressive sensing, real-time 3D integral imaging, 3D TV, photon counting imaging. To be available as an enhanced ebook with added functionality of colour films showing the effects of advanced 3D applications such as 3D microscopy, 3D biomedical imaging and 3D for security and defense applications. Acts as a single source reference to the rapidly developing field of 3D imaging technology. Provides supplementary material on a companion website including video clips, examples, numerical simulations, and experimental results to show the theoretical concepts. With contributions from leading researchers from across these fields, Multi-dimensional Imaging is a comprehensive reference for the imaging technology research community.
Book Synopsis FLIM Microscopy in Biology and Medicine by : Ammasi Periasamy
Download or read book FLIM Microscopy in Biology and Medicine written by Ammasi Periasamy and published by CRC Press. This book was released on 2009-07-06 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Detecting Signals at the Single Molecule Level: Pioneering Achievements in MicroscopyRecent advances have led to such remarkable improvements in fluorescence lifetime imaging microscopy's (FLIM) capacity for contrast and sensitivity that researchers can now employ it to detect signals at the single molecule level. FLIM also offers the additional be
Book Synopsis Advances in Imaging and Electron Physics by :
Download or read book Advances in Imaging and Electron Physics written by and published by Academic Press. This book was released on 2011-03-29 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Imaging and Electron Physics merges two long-running serials--Advances in Electronics and Electron Physics and Advances in Optical and Electron Microscopy. This series features extended articles on the physics of electron devices (especially semiconductor devices), particle optics at high and low energies, microlithography, image science and digital image processing, electromagnetic wave propagation, electron microscopy, and the computing methods used in all these domains. - Contributions from leading international scholars and industry experts - Discusses hot topic areas and presents current and future research trends - Invaluable reference and guide for physicists, engineers and mathematicians
Book Synopsis Fundamentals of Fluorescence Microscopy by : Partha Pratim Mondal
Download or read book Fundamentals of Fluorescence Microscopy written by Partha Pratim Mondal and published by Springer Science & Business Media. This book was released on 2013-12-12 with total page 219 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book starts at an introductory level and leads reader to the most advanced topics in fluorescence imaging and super-resolution techniques that have enabled new developments such as nanobioimaging, multiphoton microscopy, nanometrology and nanosensors. The interdisciplinary subject of fluorescence microscopy and imaging requires complete knowledge of imaging optics and molecular physics. So, this book approaches the subject by introducing optical imaging concepts before going in more depth about advanced imaging systems and their applications. Additionally, molecular orbital theory is the important basis to present molecular physics and gain a complete understanding of light-matter interaction at the geometrical focus. The two disciplines have some overlap since light controls the molecular states of molecules and conversely, molecular states control the emitted light. These two mechanisms together determine essential imaging factors such as, molecular cross-section, Stoke shift, emission and absorption spectra, quantum yield, signal-to-noise ratio, Forster resonance energy transfer (FRET), fluorescence recovery after photobleaching (FRAP) and fluorescence lifetime. These factors form the basis of many fluorescence based devices. The book is organized into two parts. The first part deals with basics of imaging optics and its applications. The advanced part takes care of several imaging techniques and related instrumentation that are developed in the last decade pointing towards far-field diffraction unlimited imaging.
Book Synopsis Introduction to Optical Microscopy by : Jerome Mertz
Download or read book Introduction to Optical Microscopy written by Jerome Mertz and published by Cambridge University Press. This book was released on 2019-08-01 with total page 475 pages. Available in PDF, EPUB and Kindle. Book excerpt: This fully updated, self-contained textbook covering modern optical microscopy equips students with a solid understanding of the theory underlying a range of advanced techniques. Two new chapters cover pump-probe techniques, and imaging in scattering media, and additional material throughout covers light-sheet microscopy, image scanning microscopy, and much more. An array of practical techniques are discussed, from classical phase contrast and confocal microscopy, to holographic, structured illumination, multi-photon, and coherent Raman microscopy, and optical coherence tomography. Fundamental topics are also covered, including Fourier optics, partial coherence, 3D imaging theory, statistical optics, and the physics of scattering and fluorescence. With a wealth of end-of-chapter problems, and a solutions manual for instructors available online, this is an invaluable book for electrical engineering, biomedical engineering, and physics students taking graduate courses on optical microscopy, as well as advanced undergraduates, professionals, and researchers looking for an accessible introduction to the field.
Book Synopsis Handbook of Neurophotonics by : Francesco S. Pavone
Download or read book Handbook of Neurophotonics written by Francesco S. Pavone and published by CRC Press. This book was released on 2020-05-10 with total page 579 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Handbook of Neurophotonics provides a dedicated overview of neurophotonics, covering the use of advanced optical technologies to record, stimulate, and control the activity of the brain, yielding new insight and advantages over conventional tools due to the adaptability and non-invasive nature of light. Including 32 colour figures, this book addresses functional studies of neurovascular signaling, metabolism, electrical excitation, and hemodynamics, as well as clinical applications for imaging and manipulating brain structure and function. The unifying theme throughout is not only to highlight the technology, but to show how these novel methods are becoming critical to breakthroughs that will lead to advances in our ability to manage and treat human diseases of the brain. Key Features: Provides the first dedicated book on state-of-the-art optical techniques for sensing and imaging across at the cellular, molecular, network, and whole brain levels. Highlights how the methods are used for measurement, control, and tracking of molecular events in live neuronal cells, both in basic research and clinical practice. Covers the entire spectrum of approaches, from optogenetics to functional methods, photostimulation, optical dissection, multiscale imaging, microscopy, and structural imaging. Includes chapters that show use of voltage-sensitive dye imaging, hemodynamic imaging, multiphoton imaging, temporal multiplexing, multiplane microscopy, optoacoustic imaging, near-infrared spectroscopy, and miniature neuroimaging devices to track cortical brain activity.
Book Synopsis Optics of Nanomaterials by : Vladimir I. Gavrilenko
Download or read book Optics of Nanomaterials written by Vladimir I. Gavrilenko and published by CRC Press. This book was released on 2019-11-08 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nanomaterials are mainly categorized into three groups: fundamental building blocks, dispersions or composites of building blocks in randomly ordered matrices, and spatially resolved, ordered nanostructures. Today, nanomaterials that offer some unique optical properties may find application as pure materials or may be integrated into larger structures. This book presents examples of both pure and composite materials that include organic–inorganic nanocomposites and quantum dots embedded into different matrices for various applications in modern nanotechnology. This edition has been thoroughly revised and updated with the most recent developments in the field. The newly added introductory paragraphs will help students and young researchers in better understanding the chapters. The new sections on frequently used physical constants and units conversions as well as the updated bibliography add to the book’s utility. This textbook is unique compared with its counterparts in the market in respect of its scope as it contains introductory sections to the important topics on nanomaterial optics. This feature broadens its readership from engineers and researchers working in the field of materials science and optics, to lecturers, graduate students, and beginners who want to deepen their knowledge in nanomaterial optics.
Book Synopsis Fluorescent Methods to Study Biological Membranes by : Yves Mely
Download or read book Fluorescent Methods to Study Biological Membranes written by Yves Mely and published by Springer Science & Business Media. This book was released on 2012-10-10 with total page 484 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biological membranes play a central role in cell structure, shape and functions. However, investigating the membrane bilayer has proved to be difficult due to its highly dynamic and anisotropic structure, which generates steep gradients at the nanometer scale. Due to the decisive impact of recently developed fluorescence-based techniques, tremendous advances have been made in the last few years in our understanding of membrane characteristics and functions. In this context, the present book illustrates some of these major advances by collecting review articles written by highly respected experts. The book is organized in three parts, the first of which deals with membrane probes and model membranes. The second part describes the use of advanced quantitative and high-resolution techniques to explore the properties of biological membranes, illustrating the key progress made regarding membrane organization, dynamics and interactions. The third part is focused on the investigation of membrane proteins using the same techniques, and notably on the membrane receptors that play a central role in signaling pathways and therapeutic strategies. All chapters provide comprehensive information on membranes and their exploration for beginners in the field and advanced researchers alike.
