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Guide To Molecular Cloning Techniques
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Book Synopsis Guide to Molecular Cloning Techniques by :
Download or read book Guide to Molecular Cloning Techniques written by and published by Academic Press. This book was released on 1987-09-28 with total page 858 pages. Available in PDF, EPUB and Kindle. Book excerpt: This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
Book Synopsis A Practical Guide to Molecular Cloning by : Bernard V. Perbal
Download or read book A Practical Guide to Molecular Cloning written by Bernard V. Perbal and published by Wiley-Interscience. This book was released on 1984 with total page 582 pages. Available in PDF, EPUB and Kindle. Book excerpt: Presents techniques tested at the Curie Institute and other leading labs and lists all commercially available enzymes, vectors, linkers, and other basic products for ready reference. Offers detailed explanation of protocols, allowing the isolation, cloning, and expression of genes from living species. Presents up-to-date techniques on sequencing, in vitro expression of cloned gene, and use of computers for study of nucleic acids, and is the only book that shows how to isolate DNA-protein complexes and new methods for mutagenesis of cloned genes. Contains 235 figures and 80 tables.
Book Synopsis A Practical Guide to Molecular Cloning by : Bernard Perbal
Download or read book A Practical Guide to Molecular Cloning written by Bernard Perbal and published by Wiley-Liss. This book was released on 2008-10-01 with total page 832 pages. Available in PDF, EPUB and Kindle. Book excerpt: This third edition of A Practical Guide to Molecular Cloning provides complete updates to all of the protocols, and incorporates much new material to cover a broader range of biomolecular techniques. It covers the key information required for students to perform cloning of any DNA fragment and eventually study its expression products. It also serves as an excellent comprehensive guide for any researcher using molecular techniques. Protocols in this established manual have been used successfully to characterize cellular localization, nature, and function of gene products in both prokaryotes and eukaryotes. This book is the most accessible laboratory manual for those who want to understand why techniques work and how best to apply them. Presents reliable, accurate, and clear stepwise protocols Includes all new coverage of genomics, proteomics (identification of targets and characterization of functional domains), PCR applications such as mutagenesis, and microarray technology and applications Discusses the biochemistry underlying commonly seen kits, in order that the reader will be able to use the kits to solve non–classical problems Includes new chapters on basic methods in microbiology, basic methods in cellular biology, methods for studying protein–protein interactions, SAGE, phage display, and methods for modifying the host genome Includes numerous tables and an appendix of companies and useful websites
Download or read book Cloning written by Stephen D. Fairbanks and published by Nova Publishers. This book was released on 2004 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: The terms 'recombinant DNA technology', 'DNA cloning', 'molecular cloning' or 'gene cloning' all refer to the same process: the transfer of a DNA fragment of interest from one organism to a self-replicating genetic element such as a bacterial plasmid. The DNA of interest can then be propagated in a foreign host cell. This technology has been around since the 1970s, and it has become a common practice in molecular biology labs today. Reproductive cloning is a technology used to generate an animal that has the same nuclear DNA as another currently or previously existing animal. Dolly was created by reproductive cloning technology. In a process called 'somatic cell nuclear transfer' (SCNT), scientists transfer genetic material from the nucleus of a donor adult cell to an egg whose nucleus, and thus its genetic material, has been removed. The reconstructed egg containing the DNA from a donor cell must be treated with chemicals or electric current in order to stimulate cell division. Once the cloned embryo reaches a suitable stage, it is transferred to the uterus of a female host where it continues to develop until birth. Therapeutic cloning, also called "embryo cloning," is the production of human embryos for use in research. The goal of this process is not to create cloned human beings, but rather to harvest stem cells that can be used to study human development and to treat disease. Stem cells are important to biomedical researchers because they can be used to generate virtually any type of specialised cell in the human body. This new book presents an up-to-date Chronology of Cloning along with current and selected abstracts dealing with cloning as well as a guide to books on the topic. Access to the abstract and books sections is provided by title, subject and author indexes.
Book Synopsis A Complete Guide to Gene Cloning: From Basic to Advanced by : Nayana Patil
Download or read book A Complete Guide to Gene Cloning: From Basic to Advanced written by Nayana Patil and published by Springer Nature. This book was released on 2022-04-26 with total page 177 pages. Available in PDF, EPUB and Kindle. Book excerpt: This comprehensive guide to gene cloning provides beginning and advanced readers with the background, standard techniques, practical applications, and ethical and safety considerations in the field. A one-stop reference for students, researchers in academia and industry, and anyone interested in a thorough but accessible overview.
Book Synopsis Molecular Cloning by : Joseph Sambrook
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2003 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Introduction to Molecular Cloning Techniques by : G. Lucotte
Download or read book Introduction to Molecular Cloning Techniques written by G. Lucotte and published by Wiley-VCH. This book was released on 1993-07-20 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Introduction to Molecular Cloning Techniques is a concise summary of the basis principles and methods used in genetic engineering and is intended for students and research technicians involved in microbiology, molecular biology, genetics, bioengineering, biotechnology, and chemical engineering. Focusing entirely on the most widely-used host, E. coli, the book provides extensive descriptions of cloning vectors and essential recombinant DNA methodologies, as well as discusses the steps involved in the construction of genomic, cDNA, and cosmid librairies. The chapters describe important aspects of molecular cloning by providing the necessary fundamental biochemistry and microbiology background to clearly introduce the pertinent genetic engineering concepts. Examples of routinely used experimental protocols and solved problems are included at the end of each chapter in order to extend their theoretical content and familiarize the reader with laboratory technicians and the conditions for their use.
Book Synopsis Molecular cloning by : Joseph Sambrook
Download or read book Molecular cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2001 with total page 794 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Molecular Biology Techniques by : Heather Miller
Download or read book Molecular Biology Techniques written by Heather Miller and published by Academic Press. This book was released on 2011-10-18 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual is an indispensable tool for introducing advanced undergraduates and beginning graduate students to the techniques of recombinant DNA technology, or gene cloning and expression. The techniques used in basic research and biotechnology laboratories are covered in detail. Students gain hands-on experience from start to finish in subcloning a gene into an expression vector, through purification of the recombinant protein. The third edition has been completely re-written, with new laboratory exercises and all new illustrations and text, designed for a typical 15-week semester, rather than a 4-week intensive course. The “project approach to experiments was maintained: students still follow a cloning project through to completion, culminating in the purification of recombinant protein. It takes advantage of the enhanced green fluorescent protein - students can actually visualize positive clones following IPTG induction. Cover basic concepts and techniques used in molecular biology research labs Student-tested labs proven successful in a real classroom laboratories Exercises simulate a cloning project that would be performed in a real research lab "Project" approach to experiments gives students an overview of the entire process Prep-list appendix contains necessary recipes and catalog numbers, providing staff with detailed instructions
Book Synopsis DNA Cloning and Assembly by : Sunil Chandran
Download or read book DNA Cloning and Assembly written by Sunil Chandran and published by Humana. This book was released on 2021-09-02 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive collection of DNA assembly protocols that will prove useful for any researcher interested in molecular cloning, synthetic biology, or DNA manipulation. Chapters will guide readers through computational tools to design and track the construction of DNA assemblies, workflows that enable high-throughput assembly of DNA constructs, standardized toolkits and protocols for DNA assembly, and combinatorial solutions that enable the construction and optimization of entire metabolic pathways. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, application details for both the expert and non-expert reader, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, DNA Cloning and Assembly: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Generation of cDNA Libraries by : Shao-Yao Ying
Download or read book Generation of cDNA Libraries written by Shao-Yao Ying and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its invention and subsequent development nearly 20 years ago, po- merase chain reaction (PCR) has been extensively utilized to identify numerous gene probes in vitro and in vivo. However, attempts to generate complete and full-length complementary cDNA libraries were, for the most part, fruitless and remained elusive until the last decade, when simple and rapid methods were developed. With current decoding and potential application of human genome information to genechips, there are urgent needs for identification of functional significance of these decoded gene sequences. Inherent in bringing these app- cations to fruition is the need to generate a complete and full-length cDNA library for potential functional assays of specific gene sequences. Generation of cDNA Libraries: Methods and Protocols serves as a laboratory manual on the evolution of generation of cDNA libraries, covering both ba- ground information and step-by-step practical laboratory recipes for which p- tocols, reagents, operational tips, instrumentation, and other requirements are detailed. The first chapter of the book is an overview of the basics of generating cDNA libraries, which include the following: (a) the definition of a cDNA library, (b) different kinds of cDNA libraries, (c) differences between methods for cDNA library generation using conventional approaches and novel stra- gies, including reverse generation of RNA repertoires from cDNA libraries, and (d) the quality of cDNA libraries.
Book Synopsis The Condensed Protocols from Molecular Cloning by : Joseph Sambrook
Download or read book The Condensed Protocols from Molecular Cloning written by Joseph Sambrook and published by CSHL Press. This book was released on 2006 with total page 802 pages. Available in PDF, EPUB and Kindle. Book excerpt: The Condensed Protocols From Molecular Cloning: A Laboratory Manualis a single–volume adaptation of the three–volume third edition of Molecular Cloning: A Laboratory Manual.This condensed book contains only the step–by–step portions of the protocols, accompanied by selected appendices from the world's best–selling manual of molecular biology techniques. Each protocol is cross–referenced to the appropriate pages in the original manual. This affordable companion volume, designed for bench use, offers individual investigators the opportunity to have their own personal collection of short protocols from the essential Molecular Cloning.
Book Synopsis Molecular Cloning by : Joseph Sambrook
Download or read book Molecular Cloning written by Joseph Sambrook and published by . This book was released on 2001 with total page 760 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first two editions of this manual have been mainstays of molecular biology for nearly twenty years, with an unrivalled reputation for reliability, accuracy, and clarity. In this new edition, authors Joseph Sambrook and David Russell have completely updated the book, revising every protocol and adding a mass of new material, to broaden its scope and maintain its unbeatable value for studies in genetics, molecular cell biology, developmental biology, microbiology, neuroscience, and immunology. Handsomely redesigned and presented in new bindings of proven durability, this three–volume work is essential for everyone using today’s biomolecular techniques. The opening chapters describe essential techniques, some well–established, some new, that are used every day in the best laboratories for isolating, analyzing and cloning DNA molecules, both large and small. These are followed by chapters on cDNA cloning and exon trapping, amplification of DNA, generation and use of nucleic acid probes, mutagenesis, and DNA sequencing. The concluding chapters deal with methods to screen expression libraries, express cloned genes in both prokaryotes and eukaryotic cells, analyze transcripts and proteins, and detect protein–protein interactions. The Appendix is a compendium of reagents, vectors, media, technical suppliers, kits, electronic resources and other essential information. As in earlier editions, this is the only manual that explains how to achieve success in cloning and provides a wealth of information about why techniques work, how they were first developed, and how they have evolved.
Book Synopsis Chromosome Microdissection and Cloning by : Nabil Hagag
Download or read book Chromosome Microdissection and Cloning written by Nabil Hagag and published by Academic Press. This book was released on 2012-12-02 with total page 171 pages. Available in PDF, EPUB and Kindle. Book excerpt: Chromosome Microdissection and Cloning: A Practical Guide is a straightforward guide to chromosome microdissection and cloning. It presents an overview of the procedures and briefly reviews a few areas of research in which these techniques are applied. Topics range from preparation of chromosomes for microdissection to molecular cloning of microdissected chromosomal DNA. Methods of chromosome microdissection, including video microscope method and oil chamber method, are described. Comprised of five chapters, this book begins with an overview of the structure and organization of chromosomes, followed by a description of methods for preparing and preserving chromosomal DNA in a manner that is useful for cloning and direct analysis. Microdissection of metaphase chromosomes and isolation of fragments can be accomplished in one of the three ways described in the next chapter: by microdissection using an upright microscope and glass capillaries in an oil chamber; by laser microbeam; and with the use of an inverted microscope equipped with a video camera and high magnification-high resolution lenses. A step-by-step guide to these techniques and solutions for common problems are given following each method. Protocols for cloning and identifying genetic sequences from defined chromosome regions, particularly using the polymerase chain reaction, are also discussed. The final chapter focuses on applications of chromosome microdissection, such as cloning of disease-specific genes and generating "sequence tagged sites" to be used in large DNA sequencing projects. This monograph will be particularly helpful to investigators setting up microdissection systems de novo.
Book Synopsis Understanding Dna And Gene Cloning by : Karl Drlica
Download or read book Understanding Dna And Gene Cloning written by Karl Drlica and published by Turtleback. This book was released on 2003-04-01 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies. The new edition is now completely up-to-date.
Book Synopsis Understanding DNA and Gene Cloning by : Karl Drlica
Download or read book Understanding DNA and Gene Cloning written by Karl Drlica and published by John Wiley & Sons. This book was released on 2004 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher's description: With DNA and gene cloning all over the news, readers need to understand the ongoing genetic revolution. In this highly acclaimed guide, Karl Drlica fully explains the basic science and technology readers need to understand the issues and make crucial decisions. Each step of the way he explains complex topics using easy-to-understand analogies.
Book Synopsis Guide to Molecular Cloning Techniques by :
Download or read book Guide to Molecular Cloning Techniques written by and published by Academic Press. This book was released on 1987-09-28 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This self-contained, state-of-the-art manual is designed to meet the needs of the student, experienced researcher, and newcomer to the molecular biology discipline seeking an efficient means of obtaining a clone. Available in an easy-to-use spiral-bound edition, Guide to Molecular Cloning Techniques progresses from basic techniques underlying much of recombinant DNA technology to a series of sections addressing commonly met problems.
