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Guide To Electroporation And Electrofusion
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Book Synopsis Guide to Electroporation and Electrofusion by : Donald C. Chang
Download or read book Guide to Electroporation and Electrofusion written by Donald C. Chang and published by Academic Press. This book was released on 2012-12-02 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electroporation is an efficient method to introduce macromolecules such as DNA into a wide variety of cells. Electrofusion results in the fusion of cells and can be used to produce genetic hybrids or hybridoma cells.Guide to Electroporation and Electrofusion is designed to serve the needs of students, experienced researchers, and newcomers to the field. It is a comprehensive manual that presents, in one source, up-to-date, easy-to-follow protocols necessary for efficient electroporation and electrofusion of bacteria, yeast, and plant and animal cells, as well as background information to help users optimize their results through comprehension of the principles behind these techniques. - Covers fundamentals of electroporation and electrofusion in detail: Molecular events, Mechanisms, Kinetics, Gives extensive practical information, The latest applications, Controlling parameters to maximize efficiency, Available instrumentation - Presents applications of electroporation and electrofusion in current research situations - State-of-the-art modifications to electrical pulses and generators - Application of electroporation and electrofusion to unique, alternative cell and tissue types - Gives straightforward, detailed, easy-to-follow protocols for Formation of human hybridomas - Introduction of genetic material into plant cells and pollen - Transfection of mammalian cells - Transformation of bacteria, plants, and yeast - Production of altered embryos - Optimization of electroporation by using reporter genes - Comprehensive and up-to-date - Convenient bench-top format - Approximately 125 illustrations complement the text - Complete references with article titles - Written by leading authorities in electroporation and electrofusion
Book Synopsis Guide to Electroporation and Electrofusion by : Donald Chang
Download or read book Guide to Electroporation and Electrofusion written by Donald Chang and published by Academic Press. This book was released on 1991-12-11 with total page 592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Guide to Electroporation and Electrofusion is designed to cover all relevant topics pertaining to both electroporation and electrofusion. Divided into four major parts, the book covers fundamental aspects, as well as more advance aspects of the electroporation-electrofusion relationship. The book first covers the basic principles and fundamentals by presenting the most recent theoretical and experimental studies from various fields, such as physics, chemistry, and biology. Next, the book tackles the applications of electroporation and electrofusion in biology, such as transferring, manipulating, and transforming genetic materials. In the third section, the book discusses experimental protocols to serve as a guide when performing experiments using electroporation and electrofusion. The final section discusses the instruments needed to effectively perform an experiment that involves electroporation and electrofusion. This book will be of great used to both novice and advanced researchers whose work involves electroporation and electrofusion, as it provides comprehensive information regarding these topics.
Book Synopsis Animal Cell Electroporation and Electrofusion Protocols by : Jac A. Nickoloff
Download or read book Animal Cell Electroporation and Electrofusion Protocols written by Jac A. Nickoloff and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to introduce macromolecules into animal cells, includ ing DNA, RNA, proteins, and other bioactive compounds has facili tated a broad range of biological studies, from biochemistry and biophysics to molecular biology, cell biology, and whole animal stud ies. Gene transfer technology in particular will continue to play an essential role in studies aimed at improving our understanding of the relationships between the gene structure and function, and it has impor tant practical applications in both biotechnology and biomedicine, as evidenced by the current intense interest in gene therapy. Although DNA and other macromolecules may be introduced into cells by a variety of methods, including chemical treatments and microinjection, el- troporation has proven to be simpler to perform, more efficient, and effective with a wider variety of cell types than other techniques. The early and broad success of electric field-mediated DNA transfer soon prompted researchers to investigate electroporation for transferring other types of molecules into cells, including RNA, enzymes, antibodies, and analytic dyes. Animal Cell Electroporation and Electrofusion Protocols begins with three chapters that describe the theoretical and practical aspects of electroporation, including a review of the commercially available instrumentation. These introductory chapters will be of particular inter est to those new to electric field technologies and to those developing protocols for as yet untested species or cell types. Nineteen chapters follow that present well-tested protocols for electroporation of proteins and DNA into insect, fish, and mammalian cells.
Book Synopsis Electroporation and Electrofusion in Cell Biology by : C.A. Jordan
Download or read book Electroporation and Electrofusion in Cell Biology written by C.A. Jordan and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 435 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cells can be funny. Try to grow them with a slightly wrong recipe, and they turn over and die. But hit them with an electric field strong enough to knock over a horse, and they do enough things to justify international meetings, to fill a sizable book, and to lead one to speak of an entirely new technology for cell manipulation. The very improbability of these events not only raises questions about why things happen but also leads to a long list of practical systems in which the application of strong electric fields might enable the merger of cell contents or the introduction of alien but vital material. Inevitably, the basic questions and the practical applications will not keep in step. The questions are intrinsically tough. It is hard enough to analyze the action of the relatively weak fields that rotate or align cells, but it is nearly impossible to predict responses to the cell-shredding bursts of electricity that cause them to fuse or to open up to very large molecular assemblies. Even so, theoretical studies and systematic examination of model systems have produced some creditable results, ideas which should ultimately provide hints of what to try next.
Book Synopsis Irreversible Electroporation by : Boris Rubinsky
Download or read book Irreversible Electroporation written by Boris Rubinsky and published by Springer Science & Business Media. This book was released on 2009-11-25 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: Non-thermal irreversible electroporation is a new minimally invasive surgical p- cedure with unique molecular selectivity attributes – in fact it may be considered the first clinical molecular surgery procedure. Non-thermal irreversible electro- ration is a molecular selective mode of cell ablation that employs brief electrical fields to produce nanoscale defects in the cell membrane, which can lead to cell death, without an effect on any of the other tissue molecules. The electrical fields can be produced through contact by insertion of electrode needles around the undesirable tissue and non-invasively by electromagnetic induction. This new - dition to the medical armamentarium requires the active involvement and is of interest to clinical physicians, medical researchers, mechanical engineers, che- cal engineers, electrical engineers, instrumentation designers, medical companies and many other fields and disciplines that were never exposed in their training to irreversible electroporation or to a similar concept. This edited book is designed to be a comprehensive introduction to the field of irreversible electroporation to those that were not exposed or trained in the field before and can also serve as a reference manual. Irreversible electroporation is broad and interdisciplinary. Therefore, we have made an attempt to cover every one of the various aspects of the field from an introductory basic level to state of the art.
Book Synopsis Advances in Planar Lipid Bilayers and Liposomes by : Aleš Iglič︎
Download or read book Advances in Planar Lipid Bilayers and Liposomes written by Aleš Iglič︎ and published by Academic Press. This book was released on 2012-08-06 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Planar Lipid Bilayers and Liposomes volumes cover a broad range of topics, including main arrangements of the reconstituted system, namely planar lipid bilayers as well as spherical liposomes. The invited authors present the latest results of their own research groups in this exciting multidisciplinary field. - Incorporates contributions from newcomers and established and experienced researchers - Explores the planar lipid bilayer systems and spherical liposomes from both theoretical and experimental perspectives - Serves as an indispensable source of information for new scientists
Book Synopsis Electromanipulation of Cells by : Ulrich Zimmermann
Download or read book Electromanipulation of Cells written by Ulrich Zimmermann and published by CRC Press. This book was released on 1996-02-16 with total page 422 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electromanipulation of Cells is the first comprehensive, balanced overview of this dynamic discipline. Edited by leading authorities in the field, the book surveys state-of-the-art research as well as recent practical applications of electric field technologies.
Book Synopsis Cellular Structures—Advances in Research and Application: 2013 Edition by :
Download or read book Cellular Structures—Advances in Research and Application: 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 917 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cellular Structures—Advances in Research and Application: 2013 Edition is a ScholarlyEditions™ book that delivers timely, authoritative, and comprehensive information about Intracellular Space. The editors have built Cellular Structures—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Intracellular Space in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Cellular Structures—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.
Book Synopsis Permeability and Stability of Lipid Bilayers by : E. Anibal Disalvo
Download or read book Permeability and Stability of Lipid Bilayers written by E. Anibal Disalvo and published by CRC Press. This book was released on 2017-12-14 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a comprehensive and coherent picture of how molecules diffuse across a liquid that is, on average, only two molecules thick. It begins by characterizing bilayers structurally, using X-ray diffraction, and then mechanically by measuring elastic moduli and mechanisms of failure. Emphasis is placed on the stability and mechanical properties of plant membranes that are subject to very large osmotic and thermal stresses. Using this information, the transport of molecules of increasing complexity across bilayers is analyzed.
Book Synopsis Handbook of Biological Effects of Electromagnetic Fields, Third Edition - 2 Volume Set by : Charles Polk
Download or read book Handbook of Biological Effects of Electromagnetic Fields, Third Edition - 2 Volume Set written by Charles Polk and published by CRC Press. This book was released on 1995-12-21 with total page 636 pages. Available in PDF, EPUB and Kindle. Book excerpt: The first edition of this book has been recognized as the standard reference on biological effects of electric and magnetic fields from DC to microwaves. But much has changed in this science since the book's original publication in 1986. With contributions from eighteen leading researchers, this latest edition includes authoritative discussions of many new developments and will quickly become the new, must-have resource handbook. Dielectric properties of biological tissue are thoroughly examined, followed by chapters on physical mechanisms and biological effects of static and extremely low frequency magnetic fields. New chapters on topics that were treated very briefly in the first edition now receive extensive treatment. These topics include electric and magnetic fields for bone and soft tissue repair, electroporation, and epidemiology of ELF health effects. The chapter on computer methods for predicting field intensity has been substantially revised to describe new numerical techniques developed within the last few years and includes calculations of power absorbed in the human head from cellular telephones. The chapter discussing experimental results on RF interaction with living matter now contains information on effects of very high power, very short duration pulses. A new appendix on safety standards is based on the latest publications of governmental, as well as quasi-governmental organizations (such as the U.S. Council on Radiation Protection) in the United States, Europe, and Australia. With all its revisions, this updated version of the CRC Handbook of Biological Effects of Electromagnetic Fields provides the most comprehensive overview available of this rapidly changing science.
Book Synopsis Electrotechnologies for Extraction from Food Plants and Biomaterials by : Eugene Vorobiev
Download or read book Electrotechnologies for Extraction from Food Plants and Biomaterials written by Eugene Vorobiev and published by Springer Science & Business Media. This book was released on 2009-02-28 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Recently, the electrotechnologies based on the effects of pulsed electric fields (PEF), such as ohmic heating (OH) and DC electric field, have gained real interest in the field of food processing. These techniques efficiently enhance methods of extraction from food plants and dehydration of biosolids. The PEF and pulsed OH techniques preserve the nutritional, functional, structural and sensory properties of products better than conventional extraction technologies. The electrofiltration and electro-osmotic dewatering can be very effective for the separation of bioproducts and dehydration of food wastes. The first source book in the field, this book gives an overview the fundamental principles of electrical techniques, electrophysical properties of foods and agricultural products, application of various emerging electrotechnologies for enhancing the solid-liquid separation and drying processes, extraction techniques of pigments, processing methods of different in-plant tissues and biosolids, electro-osmotic dewatering and electrofiltration of biomaterials, recent industrial- scale gains, and other aspects. Each chapter is complementary to other chapters and addresses the latest efforts in the field.
Download or read book Gene Therapeutics written by Jon A. Wolff and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 431 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the first half century of genetics, coinciding with the first half of this cen tury, geneticists dreamt of the repair of genetic disease by altering or replacing defective genes. H. J. Muller wrote of the great advantages of mutations, "nanoneedles" in his apt term, for delicately probing physiological and chemical processes. In the same spirit, genes could be used to provide treatments of needle point delicacy. Yet, during this period no realistic possibility appeared; it remained but a dream. The situation changed abruptly at the half century. Microbial genetics and its offshoot, cell culture genetics, provided the route. Pneumococcus transformation showed that exogenous DNA could become a permanent part of the genome; yet attempts to reproduce this in animals produced a few tantalizing hints of success, but mostly failures. Transduction, using a virus as mediator, offered a better op portunity. The fITSt reproducible in vivo gene therapy in a whole animal came in 1981. This was in Drosophila, with a transposable element as carrier. Flies were "cured" of a mutant eye color by incorporation of the normal allele, and the effect was transmissible, foreshadowing not only somatic, but germ line gene therapy. At the same time, retroviruses carrying human genes were found to be ex tremely efficient in transferring their contents to the chromosomes of cultured cells.
Book Synopsis Grand Challenges in Fungal Biotechnology by : Helena Nevalainen
Download or read book Grand Challenges in Fungal Biotechnology written by Helena Nevalainen and published by Springer Nature. This book was released on 2020-01-08 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a comprehensive overview of the major applications and potential of fungal biotechnology. The respective chapters report on the latest advances and opportunities in each topic area, proposing new and sustainable solutions to some of the major challenges faced by modern society. Aimed at researchers and biotechnologists in academia and industry, it represents essential reading for anyone interested in fungal biotechnology, as well as those working within the broader area of microbial biotechnology. Written in an accessible language, the book also offers a valuable reference resource for decision-makers in government and at non-governmental organizations who are involved in the development of cleaner technologies and the global bioeconomy. The 21st century is characterized by a number of critical challenges in terms of human health, developing a sustainable bioeconomy, facilitating agricultural production, and establishing practices that support a cleaner environment. While there are chemical solutions to some of these challenges, developing bio-based approaches is becoming increasingly important. Filamentous fungi, ‘the forgotten kingdom,’ are a group of unique organisms whose full potential has yet to be revealed. Some key properties, such as their exceptional capacity to secrete proteins into the external environment, have already been successfully harnessed for the production of industrial enzymes and cellulosic biofuels. Many further aspects discussed here –such as feeding the hungry with fungal protein, and the potential applications of the various small molecules produced by fungi –warrant further exploration. In turn, the book covers the use of fungal cell factories to produce foreign molecules, e.g. for therapeutics. Strategies including molecular approaches to strain improvement, and recent advances in high-throughput technologies, which are key to finding better products and producers, are also addressed. Lastly, the book discusses the advent of synthetic biology, which is destined to greatly expand the scope of fungal biotechnology. The chapter “Fungal Biotechnology in Space: Why and How?” is available open access under a Creative Commons Attribution 4.0 International License at link.springer.com.
Book Synopsis Electrical Manipulation of Cells by : Paul T. Lynch
Download or read book Electrical Manipulation of Cells written by Paul T. Lynch and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrical Manipulation of Cells provides an authoritative and up-to-date review of the field, covering all the major techniques in a single source. The book features broad coverage that ranges from the mechanisms of action of external electrical fields on biological material to the ways in which electrical stimuli are employed to manipulate cells. Bringing together the work of leading international authorities, the book covers membrane breakdown, gene delivery, electroporation, electrostimulation, cell movement, hybridoma production, plant protoplasts, electrorotation and stimulation, and electromagnetic stimulation. For each topic, the authors discuss the relevance of the approach to the current state of the art of biotechnology. Electrical Manipulation of Cells is an unmatched source of information for anyone involved in the manipulation of cells, particularly biotechnologists, cell biology, microbiologists, biophysicists and plant scientists. For researchers, the book provides technical material that ccan be employed in their own work. Students will gain thorough appreciation of the applications of this important technique.
Author :José Folgueras Méndez Publisher :Springer Science & Business Media ISBN 13 :3642211984 Total Pages :1363 pages Book Rating :4.6/5 (422 download)
Book Synopsis V Latin American Congress on Biomedical Engineering CLAIB 2011 May 16-21, 2011, Habana, Cuba by : José Folgueras Méndez
Download or read book V Latin American Congress on Biomedical Engineering CLAIB 2011 May 16-21, 2011, Habana, Cuba written by José Folgueras Méndez and published by Springer Science & Business Media. This book was released on 2012-11-06 with total page 1363 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents the proceedings of the CLAIB 2011, held in the Palacio de las Convenciones in Havana, Cuba, from 16 to 21 May 2011. The conferences of the American Congress of Biomedical Engineering are sponsored by the International Federation for Medical and Biological Engineering (IFMBE), Society for Engineering in Biology and Medicine (EMBS) and the Pan American Health Organization (PAHO), among other organizations and international agencies and bringing together scientists, academics and biomedical engineers in Latin America and other continents in an environment conducive to exchange and professional growth.
Book Synopsis Electrotransformation of Bacteria by : Natalie Eynard
Download or read book Electrotransformation of Bacteria written by Natalie Eynard and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this manual, protocols for the transformation of about 40 strains of bacteria are described, with the emphasis placed on the individual critical procedural steps, since the practical details mainly depend on the bacterial strain under investigation. This presentation together with the theoretical introductionary chapters, allows users to modify and adapt each protocol to their own experiments. Bacterial strains with relevance in the food industry, biotechnology, medical and veterinary fields, agroindustry and environmental sciences are covered.
Book Synopsis Advances in Micro/Nano Electromechanical Systems and Fabrication Technologies by : Kenichi Takahata
Download or read book Advances in Micro/Nano Electromechanical Systems and Fabrication Technologies written by Kenichi Takahata and published by BoD – Books on Demand. This book was released on 2013-05-29 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: MEMS technology is increasingly penetrating into our lives and improving our quality of life. In parallel to this, advances in nanotechnology and nanomaterials have been catalyzing the rise of NEMS. Consisting of nine chapters reviewing state-of-the-art technologies and their future trends, this book focuses on the latest development of devices and fabrication processes in the field of these extremely miniaturized electromechanical systems. The book offers new knowledge and insight into design, fabrication, and packaging, as well as solutions in these aspects for targeted applications, aiming to support scientists, engineers and academic trainees who are engaged in relevant research. In the chapters, practical issues and advances are discussed for flexible microdevices, bioMEMS, intelligent implants, optical MEMS, nanomachined structures and NEMS, and others. Most of the chapters also focus on novel fabrication/packaging processes, including silicon bulk micromachining, laser micromachining, nanolithography, and packaging for implantable microelectronics enabled by nanomaterials.
