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Author : Karl S. Matlin
Publisher : Alan R. Liss
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.3/5 (91 download)
Download or read book Functional Epithelial Cells in Culture written by Karl S. Matlin and published by Alan R. Liss. This book was released on 1989 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Mary Taub
Publisher : Springer Science & Business Media
ISBN 13 : 1468448145
Total Pages : 300 pages
Book Rating : 4.4/5 (684 download)
Download or read book Tissue Culture of Epithelial Cells written by Mary Taub and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epithelial cells are present in many different tissues in the body, and possess a diverse number of functional properties. However, all epithelial cells share some common characteristics. The cells possess a morphological polarity (an-apical and basolateral surface), and are interconnected by tight junctions. The epithelial cells also possess the capacity to transport select solutes across the monolayer. Transport systems localized on either the apical or basolateral surface are respon sible for this vectorial transport. Such characteristics of epithelial cells can be examined in the tissue culture situation. This volume discusses the use of cell culture techniques to study these fundamental properties of epithelial cells. Ma jor questions concerning epithelia which may be examined in culture are ad dressed. The approaches which are taken to answer these questions are described in detail with regards to kidney cell cultures. Similar investigations may be done with epithelial cell cultures derived from other tissues, following the kidney cell culture paradigm.
Author : Scott H. Randell
Publisher : Humana Press
ISBN 13 : 9781493956791
Total Pages : 469 pages
Book Rating : 4.9/5 (567 download)
Download or read book Epithelial Cell Culture Protocols written by Scott H. Randell and published by Humana Press. This book was released on 2016-08-23 with total page 469 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fascinating biology occurs at epithelial interfaces, whether between organism and environment or within body compartments, and many diseases inflicting huge personal and societal burdens result from dysfunction of epithelial systems, e.g., carcinomas. Epithelial cell cultures have been an integral and crucial part of the biomedical research enterprise, adding unique capabilities and enabling mechanistic approaches. In the past decade there have been many research advances, such as directed differentiation of embryonic stem cells and induced pluripotent stem cells, robotic high throughput screening, whole genome siRNA and shRNA libraries, massively parallel sequencing at low cost, identification of somatic stem cells in key organs, to name a few. Epithelial Cell Culture Protocols, Second Edition provides a cross section of up-to-date culture protocols for the most heavily studied cell systems and featured supporting technologies. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and easily accessible, Epithelial Cell Culture Protocols, Second Edition will serve outstanding investigators with the best possible information for the advancement of biomedical science.
Author :
Publisher :
ISBN 13 : 9780845133002
Total Pages : 0 pages
Book Rating : 4.1/5 (33 download)
Download or read book Modern Cell Biology: Functional epithelial cells in culture written by and published by . This book was released on 1983 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Venkataramana K Sidhaye
Publisher : Academic Press
ISBN 13 : 0128038810
Total Pages : 277 pages
Book Rating : 4.1/5 (28 download)
Download or read book Lung Epithelial Biology in the Pathogenesis of Pulmonary Disease written by Venkataramana K Sidhaye and published by Academic Press. This book was released on 2017-03-09 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lung Epithelial Biology in the Pathogenesis of Pulmonary Disease provides a one-stop resource capturing developments in lung epithelial biology related to basic physiology, pathophysiology, and links to human disease. The book provides access to knowledge of molecular and cellular aspects of lung homeostasis and repair, including the molecular basis of lung epithelial intercellular communication and lung epithelial channels and transporters. Also included is coverage of lung epithelial biology as it relates to fluid balance, basic ion/fluid molecular processes, and human disease. Useful to physician and clinical scientists, the contents of this book compile the important and most current findings about the role of epithelial cells in lung disease. Medical and graduate students, postdoctoral and clinical fellows, as well as clinicians interested in the mechanistic basis for lung disease will benefit from the books examination of principles of lung epithelium functions in physiological condition. Provides a single source of information on lung epithelial junctions and transporters Discusses of the role of the epithelium in lung homeostasis and disease Includes capsule summaries of main conclusions as well as highlights of future directions in the field Covers the mechanistic basis for lung disease for a range of audiences
Author : Kitty Verhoeckx
Publisher : Springer
ISBN 13 : 3319161040
Total Pages : 341 pages
Book Rating : 4.3/5 (191 download)
Download or read book The Impact of Food Bioactives on Health written by Kitty Verhoeckx and published by Springer. This book was released on 2015-04-29 with total page 341 pages. Available in PDF, EPUB and Kindle. Book excerpt: “Infogest” (Improving Health Properties of Food by Sharing our Knowledge on the Digestive Process) is an EU COST action/network in the domain of Food and Agriculture that will last for 4 years from April 4, 2011. Infogest aims at building an open international network of institutes undertaking multidisciplinary basic research on food digestion gathering scientists from different origins (food scientists, gut physiologists, nutritionists...). The network gathers 70 partners from academia, corresponding to a total of 29 countries. The three main scientific goals are: Identify the beneficial food components released in the gut during digestion; Support the effect of beneficial food components on human health; Promote harmonization of currently used digestion models Infogest meetings highlighted the need for a publication that would provide researchers with an insight into the advantages and disadvantages associated with the use of respective in vitro and ex vivo assays to evaluate the effects of foods and food bioactives on health. Such assays are particularly important in situations where a large number of foods/bioactives need to be screened rapidly and in a cost effective manner in order to ultimately identify lead foods/bioactives that can be the subject of in vivo assays. The book is an asset to researchers wishing to study the health benefits of their foods and food bioactives of interest and highlights which in vitro/ex vivo assays are of greatest relevance to their goals, what sort of outputs/data can be generated and, as noted above, highlight the strengths and weaknesses of the various assays. It is also an important resource for undergraduate students in the ‘food and health’ arena.
Author : C.J. Jones
Publisher : Springer Science & Business Media
ISBN 13 : 9401139059
Total Pages : 355 pages
Book Rating : 4.4/5 (11 download)
Download or read book Epithelia written by C.J. Jones and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: Epithelial cells probably constitute the most diverse group of cells found in the body. In addition to serving as interfaces between external and internal environments, their functions include ion and fluid secretion and reabsorp tion, protein exocytosis, hormone secretion, recognition, surface protection and the control of ciliary movement. By their very exposure on the surfaces of the body, epithelial cells are subjected to wide-ranging assault, by micro organisms and by chemical and physical forces. They are the targets for abrasion, infection and malignant transformation. Some epithelial cells show altered behaviour in inherited syndromes, such as cystic fibrosis, characterized by serious pancreatic and pulmonary disease. In view of the importance of epithelia and the fact that their function can be altered by environmental and inherited factors, they are the subject of intensive research, particularly so in the case of cancer where most tumours are of epithelial origin. The use of animal tissues in epithelial research continues to provide important advances and this, coupled with the need to focus more on human tissues, has prompted a greater research emphasis on accessible human epithelia and on the establishment of cell cultures from animal and human sources. For primary cell cultures and cell lines to be of value, they need to express properties appropriate to their progenitors and relevant to the study in progress.
Author : Joanna Picot
Publisher : Springer Science & Business Media
ISBN 13 : 1592598617
Total Pages : 357 pages
Book Rating : 4.5/5 (925 download)
Download or read book Human Cell Culture Protocols written by Joanna Picot and published by Springer Science & Business Media. This book was released on 2008-02-02 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: A thoroughly revised and updated collection readily reproducible techniques for culturing human cells. This new edition includes a wide range of human cell types relevant to human disease and new chapters on fibroblasts, Schwann cells, gastric and colonic epithelial cells, and parathyroid cells. The protocols follow the successful Methods in Molecular MedicineTM series format, each offering step-by-step laboratory instructions, an introduction outlining the principle behind the technique, lists of the necessary equipment and reagents, and tips on troubleshooting and avoiding known pitfalls.
Author : Mario Baratta
Publisher : Humana
ISBN 13 : 9781493993390
Total Pages : 214 pages
Book Rating : 4.9/5 (933 download)
Download or read book Epithelial Cell Culture written by Mario Baratta and published by Humana. This book was released on 2019-07-14 with total page 214 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed book explores the most current techniques to study systems and epithelial cell culture. Beginning with an overview, the volume then continues to detail systems that seek to mimic the three-dimensional organization, epithelial cells from different organs, gastrointestinal system, thyroid, salivary gland, ovary, mammary gland, and olfactory epithelial tissue. Cell culture is a fundamental technique in both medical research and drug discovery and two-dimensional (2D) culture has been the preferred method, due to the ease with which cell monolayers can be induced to proliferate on planar surfaces. The book propose several functional assay useful to test cell activities. Further, The past decades have witnessed significant efforts toward the development of three-dimensional (3D) cell cultures. Today, 3D cell cultures are emerging not only as a new tool in early drug discovery, but also as potential therapeutics to treat disease. Written for the highly successful Methods in Molecular Biology series, chapters include the kind of detail and key implementation advice that leads to excellent results in the lab.
Author : R. Ian Freshney
Publisher : John Wiley & Sons
ISBN 13 : 0471459127
Total Pages : 480 pages
Book Rating : 4.4/5 (714 download)
Download or read book Culture of Epithelial Cells written by R. Ian Freshney and published by John Wiley & Sons. This book was released on 2004-03-24 with total page 480 pages. Available in PDF, EPUB and Kindle. Book excerpt: "...a wonderful compendium of current in vitro approaches that will be a useful resource to those just starting to work with an epithelial cell system as well as those that have been working with them for years and years." --Pharmaceutical Research This completely revised and expanded new edition provides detailed descriptions of fundamental and practical aspects relating to the in vitro cultivation of disparate types of epithelia. In recent years, the use of epithelial cell culture in cell biology and tissue engineering has increased dramatically. This revision reflects those advances by including new chapters on the culture of animal and human hepatocytes, kidney epithelium, and bladder epithelium. Each chapter provides an introductory review of the principles and advantages of the particular method, followed by detailed protocols, practical tips, alternate methods, and a useful list of materials and suppliers.
Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)
Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Clare Wise
Publisher : Springer Science & Business Media
ISBN 13 : 159259185X
Total Pages : 407 pages
Book Rating : 4.5/5 (925 download)
Download or read book Epithelial Cell Culture Protocols written by Clare Wise and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 407 pages. Available in PDF, EPUB and Kindle. Book excerpt: There have been significant advances in research involving the isolation and culture of epithelial cells in the past decade, and many new techniques have been developed. Monolayer cultures can be used to evaluate the nature and behavior of cells, while the use of epithelial cells in model systems has allowed a deeper understanding of cellular and molecular mechanisms and interactions. The aim of this book is to provide a comprehensive, step-by-step guide to many techniques for epithelial cell culture, combining in one volume the more commonly used protocols along with many that are more speci- ized. Epithelial Cell Culture Protocols should help those who are new to this field and want to learn the basic culture techniques, as well as those needing to use more wide ranging and specific protocols. It should be a useful resource on its own, and also complement the other volumes that have been written about cell culture in the Methods in Molecular Biology series. Epithelial Cell Culture Protocols covers a wide variety of protocols, mostly aimed at the researcher, but also a few aimed at clinicians. The est- lishment and maintenance of primary cultures derived from many different tissues and different species is covered. Particular emphasis has been placed on protocols needed to further analyze and assess epithelial cells, for example, by looking at apoptosis and integrins and by measuring membrane capa- tance and confluence. Using different co-culture techniques, it is possible also to develop models to investigate many different systems in vitro.
Author : Ann Harris
Publisher : Cambridge University Press
ISBN 13 : 9780521559911
Total Pages : 204 pages
Book Rating : 4.5/5 (599 download)
Download or read book Epithelial Cell Culture written by Ann Harris and published by Cambridge University Press. This book was released on 1996-11-13 with total page 204 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains information and recipe-style techniques for culturing different types of epithelial cells.
Author : Blair Robert Roszell
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (119 download)
Download or read book Characterization and Functional Assessment of Lung Epithelial Cells Derived from Embryonic Stem Cells written by Blair Robert Roszell and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Alveolar epithelial type II cells participate in a host of critical activities in the distal lung including surfactant secretion, transepithelial ion transport and bacterial clearance. Preterm infants, and infants diagnosed with pulmonary hypoplasia, are born with an insufficient quantity of these cells and suffer from complications such as respiratory distress and a high risk of mortality. Cell-based therapy is emerging as a promising avenue to treat lung disease, but remains relatively unstudied for treatment of preterm lung disorders. No current methods exist for obtaining a sufficient quantity of these cells from patient biopsies since in vitro expansion is limited. A viable alternative is to derive these cells from a pluripotent cell source. Using mouse embryonic stem cells, we model differentiation of uncommitted cells to alveolar epithelial type II-like cells by recapitulating developmental cues responsible for their commitment in vivo. Still, differentiated cultures of embryonic stem cells can be highly heterogeneous and present a challenge for studying the cell type of interest. An ideal approach would be to purify a population of lung epithelial cells from a heterogeneous culture to ensure a high proportion of desirable cell types for investigation. Importantly, we must demonstrate that these ES-derived cells are similar to alveolar epithelial cells both in appearance and function. Here, I will argue the benefit of using embryonic stem-derived cells as a source of functional alveolar epithelial type II-like cells. I begin by explaining the potential use and benefits of cell-based therapy to treat lung disease, considering clinically useful cell sources and experimental models of pulmonary hypoplasia. Next, I describe the differentiation of mouse ES cells to definitive endoderm and lung epithelial cells using defined medium composition. I refine the procedure by incorporating synthetic small molecules in place of recombinant growth factors, and demonstrate their ability to prime mouse ES cells for lung epithelial differentiation. I show that heterogeneous cells can be enriched for markers of lung epithelial differentiation. Finally, I report on the progress in modifying a renal capsule allograft assay for the validation of ES-derived cells as functional lung epithelial cells.
Author : Michael S. Goligorsky
Publisher : Academic Press
ISBN 13 : 0123809290
Total Pages : 473 pages
Book Rating : 4.1/5 (238 download)
Download or read book Regenerative Nephrology written by Michael S. Goligorsky and published by Academic Press. This book was released on 2010-11-26 with total page 473 pages. Available in PDF, EPUB and Kindle. Book excerpt: Progression of chronic diseases in general and chronic kidney disease in particular has been traditionally viewed in the light of various contributors to development of glomerulosclerosis and tubulointerstitial scarring culminating in renal fibrosis. Indeed, this dogma prevailed for decades underscoring experimental attempts to halt fibrotic processes. Breakthrough investigations of the past few years on stem/progenitor cell involvement in organ regeneration caused a conceptual shift in tackling the mechanisms of nephrosclerosis. It has become clear that the rate of progression of chronic kidney disease is the net sum of the opposing trends: degenerative fibrotic processes and regenerative repair mechanisms. The latter part of this equation has been by and large ignored for years and only recently attracted investigative attention. This book revisits the problem of kidney disease by focusing on regenerative mechanisms in renal repair and on the ways these regenerative processes can become subverted by an intrinsic disease process eventuating in its progression. Cutting-edge investigations are summarized by the most experienced international team of experts. Presents a comprehensive, translational source for all aspects of renal stem cells, tissue regeneration, and stem cell therapies for renal diseases in one reference work. This will ultimately result in time savings for academic, medical and pharma researchers Experts in the renal stem cell system in kidney repair and regeneration take readers from the bench research to new therapeutic approaches, providing a common language for nephrology researchers, fellows and other stem cell researchers. This enables the discussion of development of stem cells and their use in the repair and regeneration of the kidney
Author : Leigh Ann Bovett Hoppe
Publisher :
ISBN 13 :
Total Pages : 152 pages
Book Rating : 4.:/5 (11 download)
Download or read book A Functional and Morphological Evaluation of Tissue Culture Derived Thymic Epithelial Cells in Four Mammalian Species written by Leigh Ann Bovett Hoppe and published by . This book was released on 1980 with total page 152 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Walter Birchmeier
Publisher : CRC Press
ISBN 13 : 0203303768
Total Pages : 395 pages
Book Rating : 4.2/5 (33 download)
Download or read book Epithelial Morphogenesis in Development and Disease written by Walter Birchmeier and published by CRC Press. This book was released on 2003-09-02 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bringing together a series of articles on the structural, functional, and developmental characteristics of epithelia, this volume represents a timely and valuable contribution to a growing field of study.
