Fluorescent Energy Transfer Nucleic Acid Probes

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Fluorescent Energy Transfer Nucleic Acid Probes

Author : Vladimir V. Didenko
Publisher : Humana
ISBN 13 : 9781588293800
Total Pages : 372 pages
Book Rating : 4.2/5 (938 download)

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Book Synopsis Fluorescent Energy Transfer Nucleic Acid Probes by : Vladimir V. Didenko

Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Humana. This book was released on 2006-04-01 with total page 372 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

Fluorescent Energy Transfer Nucleic Acid Probes

Author : Vladimir V. Didenko
Publisher : Springer Science & Business Media
ISBN 13 : 1597450693
Total Pages : 368 pages
Book Rating : 4.5/5 (974 download)

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Book Synopsis Fluorescent Energy Transfer Nucleic Acid Probes by : Vladimir V. Didenko

Download or read book Fluorescent Energy Transfer Nucleic Acid Probes written by Vladimir V. Didenko and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescent nucleic acid probes, which use energy transfer, include such constructs as molecular beacons, molecular break lights, Scorpion primers, TaqMan probes, and others. These probes signal detection of their targets by changing either the intensity or the color of their fluorescence. Not surpr- ingly, these luminous, multicolored probes carry more flashy names than their counterparts in the other fields of molecular biology. In recent years, fluor- cent probes and assays, which make use of energy transfer, have multiplied at a high rate and have found numerous applications. However, in spite of this explosive growth in the field, there are no manuals summarizing different p- tocols and fluorescent probe designs. In view of this, the main objective of Fluorescent Energy Transfer Nucleic Acid Probes: Designs and Protocols is to provide such a collection. Oligonucleotides with one or several chromophore tags can form fluor- cent probes capable of energy transfer. Energy transport within the probe can occur via the resonance energy transfer mechanism, also called Förster tra- fer, or by non-Förster transfer mechanisms. Although the probes using Förster transfer were developed and used first, the later non-Förster-based probes, such as molecular beacons, now represent an attractive and widely used option. The term “fluorescent energy transfer probes” in the title of this book covers both Förster-based fluorescence resonance energy transfer (FRET) probes and probes using non-FRET mechanisms. Energy transfer probes serve as molecule-size sensors, changing their fluorescence upon detection of various DNA reactions.

RNA Detection

Author : Imre Gaspar
Publisher : Humana Press
ISBN 13 : 9781493972128
Total Pages : 492 pages
Book Rating : 4.9/5 (721 download)

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Book Synopsis RNA Detection by : Imre Gaspar

Download or read book RNA Detection written by Imre Gaspar and published by Humana Press. This book was released on 2017-11-15 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume introduces different concepts and methods of detecting RNA in biological material in a variety of model systems. The chapters in this book discuss methods that will answer numerous biological questions that arise in the study of RNAs. Some of the topics covered in this book are single mRNA molecule detection in embryos and neurons; detection of mRNA and associated molecules by ISH-IEM on frozen sections; optimizing molecular beacons for intracellular analysis of RNA; imaging translation dynamics of single mRNA molecules in live cells; preparation of high-throughput sequencing libraries; and capturing RNA binding proteins in embryos and in cell-culture. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Cutting-edge and comprehensive, RNA Detection: Methods and Protocols is a valuable resource for novel and experiences scientist in the expanding field of RNAs.

Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine

Author : Shusheng Zhang
Publisher : Springer
ISBN 13 : 9811370443
Total Pages : 344 pages
Book Rating : 4.8/5 (113 download)

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Book Synopsis Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine by : Shusheng Zhang

Download or read book Nucleic Acid Amplification Strategies for Biosensing, Bioimaging and Biomedicine written by Shusheng Zhang and published by Springer. This book was released on 2019-04-25 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes the rational design, development and application of nucleic acid amplification strategies for biosensing, bioimaging and biomedicine. It consists of fifteen chapters demonstrating the use of these strategies in various areas, including fluorescence techniques, Chemiluminescence biosensors, electrochemiluminescence biosensors, colorimetric assays, surface plasmon resonance technologies, electrochemical DNA sensors, photoelectrochemical biosensor, nanopore sensors, quartz crystal microbalance, fluorescence imaging, surface-enhanced Raman spectroscopy, in vitro and in vivo metal ions detection, theranostics and microdroplet chips. Offering a collection of reviews illustrating the latest advances in biochemical analysis and therapeutics, the book shares valuable insights into current challenges and future prospects, making it a valuable resource for a wide readership in the various fields of biosensing, bioimaging and biomedicine.

The PCR Revolution

Author : Stephen A. Bustin
Publisher : Cambridge University Press
ISBN 13 : 0521882311
Total Pages : 327 pages
Book Rating : 4.5/5 (218 download)

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Book Synopsis The PCR Revolution by : Stephen A. Bustin

Download or read book The PCR Revolution written by Stephen A. Bustin and published by Cambridge University Press. This book was released on 2010 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: Examines the latest innovations and the overall impact of PCR on areas of molecular research.

Modified Nucleic Acids

Author : Kazuhiko Nakatani
Publisher : Springer
ISBN 13 : 3319271113
Total Pages : 280 pages
Book Rating : 4.3/5 (192 download)

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Book Synopsis Modified Nucleic Acids by : Kazuhiko Nakatani

Download or read book Modified Nucleic Acids written by Kazuhiko Nakatani and published by Springer. This book was released on 2016-04-04 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book spans diverse aspects of modified nucleic acids, from chemical synthesis and spectroscopy to in vivo applications, and highlights studies on chemical modifications of the backbone and nucleobases. Topics discussed include fluorescent pyrimidine and purine analogs, enzymatic approaches to the preparation of modified nucleic acids, emission and electron paramagnetic resonance (EPR) spectroscopy for studying nucleic acid structure and dynamics, non-covalent binding of low- and high-MW ligands to nucleic acids and the design of unnatural base pairs. This unique book addresses new developments and is designed for graduate level and professional research purposes.

Medical Biotechnology E-Book

Author : Judit Pongracz
Publisher : Elsevier Health Sciences
ISBN 13 : 0702050857
Total Pages : 207 pages
Book Rating : 4.7/5 (2 download)

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Book Synopsis Medical Biotechnology E-Book by : Judit Pongracz

Download or read book Medical Biotechnology E-Book written by Judit Pongracz and published by Elsevier Health Sciences. This book was released on 2008-12-11 with total page 207 pages. Available in PDF, EPUB and Kindle. Book excerpt: British Medical Association Book Awards 2009 - Commended, Basic and Clinical Sciences This textbook is aimed at medical and other health science students to explain the practical clinical impact of new techniques in biotechnology. It does not set out to explain the minutiae of the techniques themselves. The book focuses on why these techniques are useful in a clinical context and considers their potential uses, limitations and the ethical considerations that surround their use. Accessible account of subject wriiten at a level appropriate for medical students. Highly illustrated in colour. Ideal as a resource for problem-based courses. Increasing number of medical courses have modules on this subject. Suggestions for further reading.

Nucleic Acid Testing for Human Disease

Author : Attila Lorincz
Publisher : CRC Press
ISBN 13 : 1000713431
Total Pages : 462 pages
Book Rating : 4.0/5 (7 download)

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Book Synopsis Nucleic Acid Testing for Human Disease by : Attila Lorincz

Download or read book Nucleic Acid Testing for Human Disease written by Attila Lorincz and published by CRC Press. This book was released on 2016-04-19 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic Acid Testing for Human Disease describes various techniques including target and signal amplification-based NAT procedures, microarrays, bead-based multiplex assays, in situ hybridization, and SNP techniques. This book discusses RNA expression profiling and laboratory issues such as the need for proper validation of tests intended fo

Molecular Diagnostics

Author : George P. Patrinos
Publisher : Elsevier
ISBN 13 : 0080473504
Total Pages : 477 pages
Book Rating : 4.0/5 (84 download)

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Book Synopsis Molecular Diagnostics by : George P. Patrinos

Download or read book Molecular Diagnostics written by George P. Patrinos and published by Elsevier. This book was released on 2005-06-06 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Diagnostics covers current molecular biological techniques used to identify the underlying molecular defects in inherited disease. Although an increasing number of laboratories, both academic and private are moving in that direction, there are only a few books in the existing literature, and they deal only partly with diagnosis at the molecular level. Each chapter includes the principle and a brief description of the technique, followed by exmples from the authors' own epertise. Contributors are well-known experts in their field, and derive from a variety of disciplines, to ensure breadth and depth of coverage. - Examines widely used molecular biology techniques to screen for genetic defects causing inherited disorders - Includes state-of-the-art techniques for the detection of the underlying genetic heterogeneity leading to inherited disorders - Identification of genetically modified organisms (GMO's) - Forensic analysis and every-day issues in a diagnostic laboratory - Discusses ethics, genetic counselling and quality management

Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0387463127
Total Pages : 961 pages
Book Rating : 4.3/5 (874 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2007-12-05 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

Cumulated Index Medicus

Author :
Publisher :
ISBN 13 :
Total Pages : 1448 pages
Book Rating : 4.:/5 (31 download)

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Book Synopsis Cumulated Index Medicus by :

Download or read book Cumulated Index Medicus written by and published by . This book was released on 1994 with total page 1448 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Handbook of Fluorescent Dyes and Probes

Author : R. W. Sabnis
Publisher : John Wiley & Sons
ISBN 13 : 1118028694
Total Pages : 468 pages
Book Rating : 4.1/5 (18 download)

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Book Synopsis Handbook of Fluorescent Dyes and Probes by : R. W. Sabnis

Download or read book Handbook of Fluorescent Dyes and Probes written by R. W. Sabnis and published by John Wiley & Sons. This book was released on 2015-06-02 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: A COMPLETE, UP-TO-DATE RESOURCE OF INFORMATION ON MORE THAN 150 FLUORESCENT DYES AND PROBES Handbook of Fluorescent Dyes and Probes is the most comprehensive volume available on the subject, covering all the available dyes and probes known to date in the literature for uses in various fields. Top dye expert Dr. Ram Sabnis organizes the compounds alphabetically by the most commonly used chemical name. He presents an easy-to-use reference complete with novel ideas for breakthrough research in medical, biological, chemical, color, material, physical and related allied fields. The ease of use of the handbook is further enhanced by various appendixes provided at the end of the book to conveniently and easily locate the dye as per the reader's need. This is the first book to give the CAS registry numbers, chemical structure, Chemical Abstract (CA) index name, all other chemical names, Merck Index number, chemical/dye class, molecular formula, molecular weight, physical form, solubility, melting point, boiling point, pKa, absorption maxima, emission maxima, molar extinction coefficient, and quantum yield of fluorescent dyes and probes, as well as to provide access to synthetic procedures (lab scale and industrial scale) of dyes and probes in a single source. This user-friendly handbook also features references on safety, toxicity and adverse effects of dyes and probes on humans, animals and the environment, including: acute/chronic toxicity aquatic toxicity carcinogenicity cytotoxicity ecotoxicity genotoxocity hematotoxicity hepatotoxicity immunotoxicity marine toxicity microbial toxicity mutagenicity nephrotoxicity neurotoxicity nucleic acid damage oral toxicity phototoxicity phytotoxicity reproductive toxicity skin toxicity Containing imaging/labeling applications, biological/medical applications and industrial applications, Handbook of Fluorescent Dyes and Probes is a convenient, vital resource for industrial and academic researchers, and a valuable desktop reference for medical professionals, lab supervisors, scientists, chemists, biologists, engineers, physicists, intellectual property professionals, students, and professors. Includes all fluorescent dyes & probes known to date and provides a complete, up-to-date library of information in one reference/handbook Includes more than 300 fluorescent dyes & probes organized alphabetically by the commonly used Chemical Name Provides access to synthesis procedures (lab scale and industrial scale) of fluorescent dyes & probes First book to provide references on safety, toxicity and adverse effects of fluorescent dyes and probes on humans, animals, and the environment User-friendly and convenient resource guide for chemical, biological, medical, and intellectual property professionals in a broad range of disciplines

Biomedical Photonics Handbook, Second Edition

Author : Tuan Vo-Dinh
Publisher : CRC Press
ISBN 13 : 1420085166
Total Pages : 772 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Biomedical Photonics Handbook, Second Edition by : Tuan Vo-Dinh

Download or read book Biomedical Photonics Handbook, Second Edition written by Tuan Vo-Dinh and published by CRC Press. This book was released on 2014-07-29 with total page 772 pages. Available in PDF, EPUB and Kindle. Book excerpt: Shaped by Quantum Theory, Technology, and the Genomics Revolution The integration of photonics, electronics, biomaterials, and nanotechnology holds great promise for the future of medicine. This topic has recently experienced an explosive growth due to the noninvasive or minimally invasive nature and the cost-effectiveness of photonic modalities in medical diagnostics and therapy. The second edition of the Biomedical Photonics Handbook presents recent fundamental developments as well as important applications of biomedical photonics of interest to scientists, engineers, manufacturers, teachers, students, and clinical providers. The third volume, Therapeutics and Advanced Biophotonics, focuses on therapeutic modalities, advanced biophotonic technologies, and future trends. Represents the Collective Work of over 150 Scientists, Engineers, and Clinicians Designed to display the most recent advances in instrumentation and methods, as well as clinical applications in important areas of biomedical photonics to a broad audience, this three-volume handbook provides an inclusive forum that serves as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of medical technologies. What’s New in This Edition: A wide variety of photonic biochemical sensing technologies has already been developed for clinical monitoring of early disease states and physiological parameters, such as blood pressure, blood chemistry, pH, temperature, and the presence of pathological organisms or biochemical species of clinical importance. Advanced photonic detection technologies integrating the latest knowledge of genomics, proteomics, and metabolomics allow sensing of early disease states, thus revolutionizing the medicine of the future. Nanobiotechnology has opened new possibilities for detection of biomarkers of disease, imaging single molecules and in situ diagnostics at the single-cell level. In addition to these state-of-the-art advancements, the second edition contains new topics and chapters including: • Fiber Optic Probe Design • Laser and Optical Radiation Safety • Photothermal Detection • Multidimensional Fluorescence Imaging • Surface Plasmon Resonance Imaging • Molecular Contrast Optical Coherence Tomography • Multiscale Photoacoustics • Polarized Light for Medical Diagnostics • Quantitative Diffuse Reflectance Imaging • Interferometric Light Scattering • Nonlinear Interferometric Vibrational Imaging • Nanoscintillator-Based Therapy • SERS Molecular Sentinel Nanoprobes • Plasmonic Coupling Interference Nanoprobes Comprised of three books: Volume I: Fundamentals, Devices, and Techniques; Volume II: Biomedical Diagnostics; and Volume III: Therapeutics and Advanced Biophotonics, this second edition contains eight sections, and provides introductory material in each chapter. It also includes an overview of the topic, an extensive collection of spectroscopic data, and a list of references for further reading.

Fluorescent Probes

Author : Godfrey S. Beddard
Publisher :
ISBN 13 :
Total Pages : 256 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Fluorescent Probes by : Godfrey S. Beddard

Download or read book Fluorescent Probes written by Godfrey S. Beddard and published by . This book was released on 1981 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The photophysical and photochemical properties of probes in biological and in model systems are topics of intense current research. The wavelength, intensity, time and polarisation dependence of fluorescence probes can provide information on properties as diverse as electric fields and changes in conformation and may be used in the formidable task of establishing structure-function relationships in proteins and cell membranes. The wide scope of interactions which can be studied by these fluorescent molecules is illustrated in the articles in this volume which are based on talks, although much extended and updated, given at a meeting on 'Fluorescence Probes in Proteins and Membranes' held in the Royal Institution in November 1979.

DNA Technology

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306473879
Total Pages : 339 pages
Book Rating : 4.3/5 (64 download)

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Book Synopsis DNA Technology by : Joseph R. Lakowicz

Download or read book DNA Technology written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 1991 with total page 339 pages. Available in PDF, EPUB and Kindle. Book excerpt: Annotation. In this inaugural volume of a new series, experts in the field help biochemists, analytical chemists, spectroscopists, biophysicists, and other specialists keep up with the latest techniques and technologies available in fluorescence spectroscopy.

Nucleic Acid Probes—Advances in Research and Application: 2013 Edition

Author :
Publisher : ScholarlyEditions
ISBN 13 : 1481690450
Total Pages : 135 pages
Book Rating : 4.4/5 (816 download)

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Book Synopsis Nucleic Acid Probes—Advances in Research and Application: 2013 Edition by :

Download or read book Nucleic Acid Probes—Advances in Research and Application: 2013 Edition written by and published by ScholarlyEditions. This book was released on 2013-06-21 with total page 135 pages. Available in PDF, EPUB and Kindle. Book excerpt: Nucleic Acid Probes—Advances in Research and Application: 2013 Edition is a ScholarlyBrief™ that delivers timely, authoritative, comprehensive, and specialized information about Oligonucleotide Probes in a concise format. The editors have built Nucleic Acid Probes—Advances in Research and Application: 2013 Edition on the vast information databases of ScholarlyNews.™ You can expect the information about Oligonucleotide Probes in this book to be deeper than what you can access anywhere else, as well as consistently reliable, authoritative, informed, and relevant. The content of Nucleic Acid Probes—Advances in Research and Application: 2013 Edition has been produced by the world’s leading scientists, engineers, analysts, research institutions, and companies. All of the content is from peer-reviewed sources, and all of it is written, assembled, and edited by the editors at ScholarlyEditions™ and available exclusively from us. You now have a source you can cite with authority, confidence, and credibility. More information is available at http://www.ScholarlyEditions.com/.

Introduction to Fluorescence Sensing

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
ISBN 13 : 140209003X
Total Pages : 606 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Introduction to Fluorescence Sensing by : Alexander P. Demchenko

Download or read book Introduction to Fluorescence Sensing written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2008-12-21 with total page 606 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence sensing is a rapidly developing field of research and technology. Its target is nearly the whole world of natural and synthetic compounds being detected in different media including living bodies. The application area range from control of industrial processes to environment monitoring and clinical diagnostics. Among different detection methods fluorescence techniques are distinguished by ultimate sensitivity, high temporal and spatial resolution and versatility that allows not only remote detection of different targets but their imaging within the living cells. The basic mechanism of sensing is the transmission of the signal produced by molecular interaction with the target to fluorescent molecules, nanoparticles and nanocomposites with the detection by devices based on modern electronics and optics. In this interdisciplinary field of research and development the book is primarily intended to be a guide for students and young researchers. It is also addressed to professionals involved in active research and product development serving as a reference for the recent achievements. The users of these products will find description of principles that could allow proper selection of sensors for particular needs. Making a strong link between education, research and product development, this book discusses future directions.