Fluorescence Spectroscopic Studies Of Protein Conformational Dynamics

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Protein Conformational Dynamics

Author : Ke-li Han
Publisher : Springer Science & Business Media
ISBN 13 : 3319029703
Total Pages : 488 pages
Book Rating : 4.3/5 (19 download)

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Book Synopsis Protein Conformational Dynamics by : Ke-li Han

Download or read book Protein Conformational Dynamics written by Ke-li Han and published by Springer Science & Business Media. This book was released on 2014-01-20 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book discusses how biological molecules exert their function and regulate biological processes, with a clear focus on how conformational dynamics of proteins are critical in this respect. In the last decade, the advancements in computational biology, nuclear magnetic resonance including paramagnetic relaxation enhancement, and fluorescence-based ensemble/single-molecule techniques have shown that biological molecules (proteins, DNAs and RNAs) fluctuate under equilibrium conditions. The conformational and energetic spaces that these fluctuations explore likely contain active conformations that are critical for their function. More interestingly, these fluctuations can respond actively to external cues, which introduces layers of tight regulation on the biological processes that they dictate. A growing number of studies have suggested that conformational dynamics of proteins govern their role in regulating biological functions, examples of this regulation can be found in signal transduction, molecular recognition, apoptosis, protein / ion / other molecules translocation and gene expression. On the experimental side, the technical advances have offered deep insights into the conformational motions of a number of proteins. These studies greatly enrich our knowledge of the interplay between structure and function. On the theoretical side, novel approaches and detailed computational simulations have provided powerful tools in the study of enzyme catalysis, protein / drug design, protein / ion / other molecule translocation and protein folding/aggregation, to name but a few. This work contains detailed information, not only on the conformational motions of biological systems, but also on the potential governing forces of conformational dynamics (transient interactions, chemical and physical origins, thermodynamic properties). New developments in computational simulations will greatly enhance our understanding of how these molecules function in various biological events.

Single Molecule Dynamics in Life Science

Author : Toshio Yanagida
Publisher : John Wiley & Sons
ISBN 13 : 3527312889
Total Pages : 347 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Single Molecule Dynamics in Life Science by : Toshio Yanagida

Download or read book Single Molecule Dynamics in Life Science written by Toshio Yanagida and published by John Wiley & Sons. This book was released on 2008-11-24 with total page 347 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this first comprehensive resource to cover the application of single molecule techniques to biological measurements, the pioneers in the field show how to both set up and interpret a single molecule experiment. Following an introduction to single molecule measurements and enzymology, the expert authors consider molecular motors and mechanical properties before moving on to the applications themselves. Detailed discussions of studies on protein enzymes, ribozymes and nucleic acids are also included.

Protein Folding Dynamics and Stability

Author : Prakash Saudagar
Publisher : Springer Nature
ISBN 13 : 9819920795
Total Pages : 287 pages
Book Rating : 4.8/5 (199 download)

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Book Synopsis Protein Folding Dynamics and Stability by : Prakash Saudagar

Download or read book Protein Folding Dynamics and Stability written by Prakash Saudagar and published by Springer Nature. This book was released on 2023-05-27 with total page 287 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes recent important advancements in protein folding dynamics and stability research, as well as explaining fundamentals and examining potential methodological approaches in protein science. In vitro, in silico, and in vivo method based research of how the stability and folding of proteins help regulate the cellular dynamics and impact cell function that are crucial in explaining various physiological and pathological processes. This book offers a comprehensive coverage on various techniques and related recent developments in the experimental and computational methods of protein folding, dynamics, and stability studies. The book is also structured in such a way as to summarize the latest developments in the fiddle and key concepts to ensure that readers can understand advanced concepts as well as the fundamental big picture. And most of all, fresh insights are provided into the convergence of protein science and technology. Protein Folding Dynamics and Stability is an ideal guide to the field that will be of value for all levels of researchers and advanced graduate students with training in biochemical laboratory research.

Principles of Fluorescence Spectroscopy

Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0387463127
Total Pages : 961 pages
Book Rating : 4.3/5 (874 download)

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Book Synopsis Principles of Fluorescence Spectroscopy by : Joseph R. Lakowicz

Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2007-12-05 with total page 961 pages. Available in PDF, EPUB and Kindle. Book excerpt: The third edition of this established classic text reference builds upon the strengths of its very popular predecessors. Organized as a broadly useful textbook Principles of Fluorescence Spectroscopy, 3rd edition maintains its emphasis on basics, while updating the examples to include recent results from the scientific literature. The third edition includes new chapters on single molecule detection, fluorescence correlation spectroscopy, novel probes and radiative decay engineering. Includes a link to Springer Extras to download files reproducing all book artwork, for easy use in lecture slides. This is an essential volume for students, researchers, and industry professionals in biophysics, biochemistry, biotechnology, bioengineering, biology and medicine.

New Trends in Fluorescence Spectroscopy

Author : Bernard Valeur
Publisher : Springer Science & Business Media
ISBN 13 : 364256853X
Total Pages : 496 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis New Trends in Fluorescence Spectroscopy by : Bernard Valeur

Download or read book New Trends in Fluorescence Spectroscopy written by Bernard Valeur and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 496 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first volume in the new Springer Series on Fluorescence brings together fundamental and applied research from this highly interdisciplinary and field, ranging from chemistry and physics to biology and medicine. Special attention is given to supramolecular systems, sensor applications, confocal microscopy and protein-protein interactions. This carefully edited collection of articles is an invaluable tool for practitioners and novices.

Biomedical Index to PHS-supported Research

Author :
Publisher :
ISBN 13 :
Total Pages : 1060 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Biomedical Index to PHS-supported Research by :

Download or read book Biomedical Index to PHS-supported Research written by and published by . This book was released on 1990 with total page 1060 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Comprehensive Biophysics

Author :
Publisher : Academic Press
ISBN 13 : 0080957188
Total Pages : 3533 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Comprehensive Biophysics by :

Download or read book Comprehensive Biophysics written by and published by Academic Press. This book was released on 2012-04-12 with total page 3533 pages. Available in PDF, EPUB and Kindle. Book excerpt: Biophysics is a rapidly-evolving interdisciplinary science that applies theories and methods of the physical sciences to questions of biology. Biophysics encompasses many disciplines, including physics, chemistry, mathematics, biology, biochemistry, medicine, pharmacology, physiology, and neuroscience, and it is essential that scientists working in these varied fields are able to understand each other's research. Comprehensive Biophysics, Nine Volume Set will help bridge that communication gap. Written by a team of researchers at the forefront of their respective fields, under the guidance of Chief Editor Edward Egelman, Comprehensive Biophysics, Nine Volume Set provides definitive introductions to a broad array of topics, uniting different areas of biophysics research - from the physical techniques for studying macromolecular structure to protein folding, muscle and molecular motors, cell biophysics, bioenergetics and more. The result is this comprehensive scientific resource - a valuable tool both for helping researchers come to grips quickly with material from related biophysics fields outside their areas of expertise, and for reinforcing their existing knowledge. Biophysical research today encompasses many areas of biology. These studies do not necessarily share a unique identifying factor. This work unites the different areas of research and allows users, regardless of their background, to navigate through the most essential concepts with ease, saving them time and vastly improving their understanding The field of biophysics counts several journals that are directly and indirectly concerned with the field. There is no reference work that encompasses the entire field and unites the different areas of research through deep foundational reviews. Comprehensive Biophysics fills this vacuum, being a definitive work on biophysics. It will help users apply context to the diverse journal literature offering, and aid them in identifying areas for further research Chief Editor Edward Egelman (E-I-C, Biophysical Journal) has assembled an impressive, world-class team of Volume Editors and Contributing Authors. Each chapter has been painstakingly reviewed and checked for consistent high quality. The result is an authoritative overview which ties the literature together and provides the user with a reliable background information and citation resource

Topics in Fluorescence Spectroscopy: Biochemical applications

Author : Joseph R. Lakowicz
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (91 download)

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Book Synopsis Topics in Fluorescence Spectroscopy: Biochemical applications by : Joseph R. Lakowicz

Download or read book Topics in Fluorescence Spectroscopy: Biochemical applications written by Joseph R. Lakowicz and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Fluorescence Fluctuation Spectroscopy (FFS) Part B

Author :
Publisher : Academic Press
ISBN 13 : 0124058574
Total Pages : 395 pages
Book Rating : 4.1/5 (24 download)

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Book Synopsis Fluorescence Fluctuation Spectroscopy (FFS) Part B by :

Download or read book Fluorescence Fluctuation Spectroscopy (FFS) Part B written by and published by Academic Press. This book was released on 2012-12-29 with total page 395 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new volume of Methods in Enzymology continues the legacy of this premier serial with quality chapters authored by leaders in the field. This volume covers fluorescence fluctuation spectroscopy and includes chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells. - Continues the legacy of this premier serial with quality chapters authored by leaders in the field - Covers fluorescence fluctuation spectroscopy - Contains chapters on such topics as Förster resonance energy transfer (fret) with fluctuation algorithms, protein corona on nanoparticles by FCS, and FFS approaches to the study of receptors in live cells

Single Molecule Spectroscopy in Chemistry, Physics and Biology

Author : Astrid Graslund
Publisher : Springer Science & Business Media
ISBN 13 : 3642025986
Total Pages : 570 pages
Book Rating : 4.6/5 (42 download)

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Book Synopsis Single Molecule Spectroscopy in Chemistry, Physics and Biology by : Astrid Graslund

Download or read book Single Molecule Spectroscopy in Chemistry, Physics and Biology written by Astrid Graslund and published by Springer Science & Business Media. This book was released on 2010-04-17 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.

Advances in Protein Molecular and Structural Biology Methods

Author : Timir Tripathi
Publisher : Academic Press
ISBN 13 : 0323902650
Total Pages : 716 pages
Book Rating : 4.3/5 (239 download)

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Book Synopsis Advances in Protein Molecular and Structural Biology Methods by : Timir Tripathi

Download or read book Advances in Protein Molecular and Structural Biology Methods written by Timir Tripathi and published by Academic Press. This book was released on 2022-01-14 with total page 716 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Protein Molecular and Structural Biology Methods offers a complete overview of the latest tools and methods applicable to the study of proteins at the molecular and structural level. The book begins with sections exploring tools to optimize recombinant protein expression and biophysical techniques such as fluorescence spectroscopy, NMR, mass spectrometry, cryo-electron microscopy, and X-ray crystallography. It then moves towards computational approaches, considering structural bioinformatics, molecular dynamics simulations, and deep machine learning technologies. The book also covers methods applied to intrinsically disordered proteins (IDPs)followed by chapters on protein interaction networks, protein function, and protein design and engineering. It provides researchers with an extensive toolkit of methods and techniques to draw from when conducting their own experimental work, taking them from foundational concepts to practical application. - Presents a thorough overview of the latest and emerging methods and technologies for protein study - Explores biophysical techniques, including nuclear magnetic resonance, X-ray crystallography, and cryo-electron microscopy - Includes computational and machine learning methods - Features a section dedicated to tools and techniques specific to studying intrinsically disordered proteins

Proteins: Structure, Function, and Engineering

Author : B.B. Biswas
Publisher : Springer
ISBN 13 : 9781489917294
Total Pages : 436 pages
Book Rating : 4.9/5 (172 download)

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Book Synopsis Proteins: Structure, Function, and Engineering by : B.B. Biswas

Download or read book Proteins: Structure, Function, and Engineering written by B.B. Biswas and published by Springer. This book was released on 2013-05-24 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Here, researchers review the latest breakthroughs in protein research. Their contributions explore emerging principles and techniques and survey important classes of proteins that will play key roles in the field's future. Articles examine the possibility of a Boltzman-like distribution in protein substructures, the new technique of Raman spectroscopy, and compact intermediate states of protein folding. This well-illustrated volume also features coverage of proteins that bind nucleic acids.

Ultraviolet Spectroscopy of Proteins

Author : Alexander P. Demchenko
Publisher : Springer Science & Business Media
ISBN 13 : 3642708471
Total Pages : 323 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Ultraviolet Spectroscopy of Proteins by : Alexander P. Demchenko

Download or read book Ultraviolet Spectroscopy of Proteins written by Alexander P. Demchenko and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 323 pages. Available in PDF, EPUB and Kindle. Book excerpt: The aim of this book is to give a comprehensive description of the basic methods used in the ultraviolet spectroscopy of proteins, to discuss new trends and development of these methods, and to analyze their different applications in the study of various aspects of protein structure and dynamics. Ultraviolet spectroscopy is one of the oldest and most popular methods in the field of biochemistry and molecular biophysics. At present, it is difficult to imagine the biochemical laboratory without a recording spectrophotometer or spectrofluorimeter. There are several hundreds of publications directly devoted to protein ultraviolet spectroscopy and in a great number of studies UV spectroscopic methods are used for the structural analysis of different proteins. Meanwhile a unified description of the theoretical basis of the methods, experimental techniques, data analysis, and generalization of results obtained in solving the specific problems of protein structure are lacking. There are three reasons for which a monograph on ultraviolet spectroscopy is needed today. Firstly, there has been significant growth in facilities of experimental technique, its precision, and versatility associated with computer data analysts. This new technique is available to a wide circle of scientists engaged in the field of protein research. Most of them are not spectroscopists and, thus, there is a need for a conceivable and precise source of information on how to use this method and what kind of data it should provide.

Modern Optical Spectroscopy

Author : William W. Parson
Publisher : Springer Science & Business Media
ISBN 13 : 3540375422
Total Pages : 512 pages
Book Rating : 4.5/5 (43 download)

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Book Synopsis Modern Optical Spectroscopy by : William W. Parson

Download or read book Modern Optical Spectroscopy written by William W. Parson and published by Springer Science & Business Media. This book was released on 2007-02-20 with total page 512 pages. Available in PDF, EPUB and Kindle. Book excerpt: The student edition of Modern Optical Spectroscopy includes a new set of exercises for each chapter. The exercises and problems generally emphasize basic points, and often include simpli?ed absorption or emission spectra or molecular orbitals that can be evaluated easily with the aid of a calculator or spreadsheet. Students who are adept at computer programming will ?nd it instructive to try to write algorithms that also could be applied to larger, more complicated sets of data. Spectraintroducedinsomeofthe problems forChaps.4and5areusedagain in later chapters to illustrate how quantities calculated from the spectra can be applied to topics such as resonance energy transfer and exciton interactions. Seattle, November, 2008 William W. Parson Preface This book began as lecture notes for a course on optical spectroscopy that I taught for graduate students in biochemistry, chemistry, and our interdisciplinary programs in molecular biophysics and biomolecular structure and design. I started expanding the notes partly to try to illuminate the stream of new experimental information on photosynthetic antennas and reaction centers, but mostly just for fun. I hope that readers will ?nd the results not only useful, but also as stimulating as I have.

Quantitative Biology

Author : Michael E. Wall
Publisher : CRC Press
ISBN 13 : 1439827222
Total Pages : 406 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Quantitative Biology by : Michael E. Wall

Download or read book Quantitative Biology written by Michael E. Wall and published by CRC Press. This book was released on 2012-08-25 with total page 406 pages. Available in PDF, EPUB and Kindle. Book excerpt: Quantitative methods are revolutionizing modern molecular and cellular biology. Groundbreaking technical advances are fueling the rapid expansion in our ability to observe, as seen in multidisciplinary studies that integrate theory, computation, experimental assays, and the control of microenvironments. Integrating new experimental and theoretical methods, Quantitative Biology: From Molecular to Cellular Systems gives both new and established researchers a solid foundation for starting work in this field. The book is organized into three sections: Fundamental Concepts covers bold ideas that inspire novel approaches in modern quantitative biology. It offers perspectives on evolutionary dynamics, system design principles, chance and memory, and information processing in biology. Methods describes recently developed or improved techniques that are transforming biological research. It covers experimental methods for studying single-molecule biochemistry, small-angle scattering from biomolecules, subcellular localization of proteins, and single-cell behavior. It also describes theoretical methods for synthetic biology and modeling random variations among cells. Molecular and Cellular Systems focuses on specific biological systems where modern quantitative biology methods are making an impact. It incorporates case studies of biological systems for which new concepts or methods are increasing our understanding. Examples include protein kinase at the molecular level, the genetic switch of phage lambda at the regulatory system level, and Escherichia coli chemotaxis at the cellular level. In short, Quantitative Biology presents practical tools for the observation, modeling, design, and manipulation of biological systems from the molecular to the cellular levels.

Nanotechnology in Biology and Medicine

Author : Tuan Vo-Dinh
Publisher : CRC Press
ISBN 13 : 1439893799
Total Pages : 740 pages
Book Rating : 4.4/5 (398 download)

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Book Synopsis Nanotechnology in Biology and Medicine by : Tuan Vo-Dinh

Download or read book Nanotechnology in Biology and Medicine written by Tuan Vo-Dinh and published by CRC Press. This book was released on 2017-10-03 with total page 740 pages. Available in PDF, EPUB and Kindle. Book excerpt: The second edition of Nanotechnology in Biology and Medicine is intended to serve as an authoritative reference source for a broad audience involved in the research, teaching, learning, and practice of nanotechnology in life sciences. This technology, which is on the scale of molecules, has enabled the development of devices smaller and more efficient than anything currently available. To understand complex biological nanosystems at the cellular level, we urgently need to develop a next-generation nanotechnology tool kit. It is believed that the new advances in genetic engineering, genomics, proteomics, medicine, and biotechnology will depend on our mastering of nanotechnology in the coming decades. The integration of nanotechnology, material sciences, molecular biology, and medicine opens the possibility of detecting and manipulating atoms and molecules using nanodevices, which have the potential for a wide variety of biological research topics and medical uses at the cellular level. This book presents the most recent scientific and technological advances of nanotechnology for use in biology and medicine. Each chapter provides introductory material with an overview of the topic of interest; a description of methods, protocols, instrumentation, and applications; and a collection of published data with an extensive list of references for further details. The goal of this book is to provide a comprehensive overview of the most recent advances in instrumentation, methods, and applications in areas of nanobiotechnology, integrating interdisciplinary research and development of interest to scientists, engineers, manufacturers, teachers, and students.

Handbook of Single-Molecule Biophysics

Author : Peter Hinterdorfer
Publisher : Springer Science & Business Media
ISBN 13 : 0387764976
Total Pages : 634 pages
Book Rating : 4.3/5 (877 download)

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Book Synopsis Handbook of Single-Molecule Biophysics by : Peter Hinterdorfer

Download or read book Handbook of Single-Molecule Biophysics written by Peter Hinterdorfer and published by Springer Science & Business Media. This book was released on 2009-12-24 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This handbook describes experimental techniques to monitor and manipulate individual biomolecules, including fluorescence detection, atomic force microscopy, and optical and magnetic trapping. It includes single-molecule studies of physical properties of biomolecules such as folding, polymer physics of protein and DNA, enzymology and biochemistry, single molecules in the membrane, and single-molecule techniques in living cells.