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Author : Robert F. McGuire
Publisher :
ISBN 13 :
Total Pages : 298 pages
Book Rating : 4.:/5 (89 download)
Download or read book Fluorescence and Polarization of Fluorescence of Protein Solutions written by Robert F. McGuire and published by . This book was released on 1968 with total page 298 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Sergei V. Konev
Publisher : Springer Science & Business Media
ISBN 13 : 1468407007
Total Pages : 208 pages
Book Rating : 4.4/5 (684 download)
Download or read book Fluorescence and Phosphorescence of Proteins and Nucleic Acids written by Sergei V. Konev and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 208 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence and phosphorescence are proving to be extremely sensitive probes for elucidating conformation of proteins and nucleic acids and for studying molecular interactions. Newer instrumentation and techniques hold forth great promise for the future of these luminescence methods in biopolymer research. It must be noted, however, that the discovery that certain amino acids, purines, and pyrimidines emit fluorescence or phosphorescence is relatively recent, occurring within the last decade. Professor Konev is one of the pioneers in the application of these procedures to biopolymers and is highly qualified to write about this subject. This book, though written largely as a monograph of the author's own contributions, is also an excellent review of the subject. Of particular interest are the references to many important Russian papers in this field which have not been recognized in the Western literature. It is apparent from this book that fluorescence and phosphorescence methods are being used about as widely in Russia as elsewhere in the world and that we must not overlook these im portant contributions. Konev's studies on protein fluorescence have been widely recognized. It is of interest to learn about these and other of his applications. The last part of the book, which deals with fluorescence as a means to probe into the structure and conforma tion of macromolecules in intact cells, is most interesting. Aside from published symposia this book is the first written specifically about luminescence of biopolymers. Sidney Udenfriend Bethesda. Maryland May, 1967 v CONTENTS Introduction . • • . . . . . . . . . • . . . . . . . . . .
Author : Kevin F. Sullivan
Publisher : Elsevier
ISBN 13 : 0080557244
Total Pages : 613 pages
Book Rating : 4.0/5 (85 download)
Download or read book Fluorescent Proteins written by Kevin F. Sullivan and published by Elsevier. This book was released on 2007-12-14 with total page 613 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition of Fluorescent Proteins presents current applications of autofluorescent proteins in cell and molecular biology authored by researchers from many of the key laboratories in the field. Starting from a current review of the broad palette of fluorescent proteins available, several chapters focus on key autofluorescent protein variants, including spectral variants, photodynamic variants as well as chimeric FP approaches. Molecular applications are addressed in chapters that detail work with single molecules, approaches to generating protein fusions and biosensors as well as analysis of protein-protein interactions in vivo by FRET, fluorescence polarization and fluorescence cross correlation techniques. A number of approaches to in vivo dynamics are presented, including FRAP, photoactivation, and 4-dimensional microscopy. Behavior of spindle components, membrane proteins, mRNA trafficking as well as analysis of cell types in tissues and in development are detailed and provide models for a wide variety of experimental approaches. In addition, several chapters deal directly with the computational issues involved in processing multidimensional image data and using fluorescent imaging to probe cellular behavior with quantitative modeling. This volume brings together the latest perspective and techniques on fluorescent proteins and will be an invaluable reference in a wide range of laboratories.
Author : Joseph R. Lacowicz
Publisher : Springer Science & Business Media
ISBN 13 : 0306471027
Total Pages : 320 pages
Book Rating : 4.3/5 (64 download)
Download or read book Protein Fluorescence written by Joseph R. Lacowicz and published by Springer Science & Business Media. This book was released on 2006-04-18 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intrinsic or natural fluorescence of proteins is perhaps the most complex area of biochemical fluorescence. Fortunately the fluorescent amino acids, phenylalanine, tyrosine and tryptophan are relatively rare in proteins. Tr- tophan is the dominant intrinsic fluorophore and is present at about one mole % in protein. As a result most proteins contain several tryptophan residues and even more tyrosine residues. The emission of each residue is affected by several excited state processes including spectral relaxation, proton loss for tyrosine, rotational motions and the presence of nearby quenching groups on the protein. Additionally, the tyrosine and tryptophan residues can interact with each other by resonance energy transfer (RET) decreasing the tyrosine emission. In this sense a protein is similar to a three-particle or mul- particle problem in quantum mechanics where the interaction between particles precludes an exact description of the system. In comparison, it has been easier to interpret the fluorescence data from labeled proteins because the fluorophore density and locations could be controlled so the probes did not interact with each other. From the origins of biochemical fluorescence in the 1950s with Prof- sor G. Weber until the mid-1980s, intrinsic protein fluorescence was more qualitative than quantitative. An early report in 1976 by A. Grindvald and I. Z. Steinberg described protein intensity decays to be multi-exponential. Attempts to resolve these decays into the contributions of individual tryp- phan residues were mostly unsuccessful due to the difficulties in resolving closely spaced lifetimes.
Author : Weibo Cai
Publisher : BoD – Books on Demand
ISBN 13 : 9535103970
Total Pages : 488 pages
Book Rating : 4.5/5 (351 download)
Download or read book Protein-Protein Interactions written by Weibo Cai and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.
Author : Roger Mallinson
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (271 download)
Download or read book Steady State Fluorescence and Fluorescence Polarization Studies of Aqueous Solutions of Indole, Tryptophan and the Protein -lactoglobulin written by Roger Mallinson and published by . This book was released on 1979 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Joseph R. Lakowicz
Publisher : Springer Science & Business Media
ISBN 13 : 146157658X
Total Pages : 487 pages
Book Rating : 4.4/5 (615 download)
Download or read book Principles of Fluorescence Spectroscopy written by Joseph R. Lakowicz and published by Springer Science & Business Media. This book was released on 2013-11-11 with total page 487 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence methods are being used increasingly in biochemical, medical, and chemical research. This is because of the inherent sensitivity of this technique. and the favorable time scale of the phenomenon of fluorescence. 8 Fluorescence emission occurs about 10- sec (10 nsec) after light absorp tion. During this period of time a wide range of molecular processes can occur, and these can effect the spectral characteristics of the fluorescent compound. This combination of sensitivity and a favorable time scale allows fluorescence methods to be generally useful for studies of proteins and membranes and their interactions with other macromolecules. This book describes the fundamental aspects of fluorescence. and the biochemical applications of this methodology. Each chapter starts with the -theoreticalbasis of each phenomenon of fluorescence, followed by examples which illustrate the use of the phenomenon in the study of biochemical problems. The book contains numerous figures. It is felt that such graphical presentations contribute to pleasurable reading and increased understand ing. Separate chapters are devoted to fluorescence polarization, lifetimes, quenching, energy transfer, solvent effects, and excited state reactions. To enhance the usefulness of this work as a textbook, problems are included which illustrate the concepts described in each chapter. Furthermore, a separate chapter is devoted to the instrumentation used in fluorescence spectroscopy. This chapter will be especially valuable for those perform ing or contemplating fluorescence measurements. Such measurements are easily compromised by failure to consider a number of simple principles.
Author : Brian S. Everitt
Publisher : CRC Press
ISBN 13 : 9781420057492
Total Pages : 260 pages
Book Rating : 4.0/5 (574 download)
Download or read book A Handbook of Statistical Analyses Using S-PLUS written by Brian S. Everitt and published by CRC Press. This book was released on 2019-05-07 with total page 260 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of this book was published, S-PLUS has evolved markedly with new methods of analysis, new graphical procedures, and a convenient graphical user interface (GUI). Today, S-PLUS is the statistical software of choice for many applied researchers in disciplines ranging from finance to medicine. Combining the command line languag
Author : Martin Hof
Publisher : Springer Science & Business Media
ISBN 13 : 3540270043
Total Pages : 315 pages
Book Rating : 4.5/5 (42 download)
Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2006-01-27 with total page 315 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.
Author : R. Mallinson
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (599 download)
Download or read book Steady State Fluorescence and Fluorescence Polarization Studies of Aqueous Solutions of Indole, Tryphopan, Protein B - Lactoglobulin written by R. Mallinson and published by . This book was released on 1980 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : David M. Jameson
Publisher : Springer
ISBN 13 : 3319413287
Total Pages : 352 pages
Book Rating : 4.3/5 (194 download)
Download or read book Perspectives on Fluorescence written by David M. Jameson and published by Springer. This book was released on 2016-08-08 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gregorio Weber is widely acknowledged as the person responsible for the advent of modern fluorescence spectroscopy. Since 2016 is the 100th anniversary of Gregorio Weber’s birth, this special volume has been prepared to honor his life and achievements. It offers contributions from outstanding researchers in the fluorescence field, describing their perspectives on modern fluorescence and its highly diverse applications, ranging from the photophysics of tryptophan and proteins, membrane studies, fluorescence microscopy on live cells, novel software approaches and instrumentation. Many of the authors knew Gregorio Weber personally and have shared their impressions of the man and his contributions. This volume appeals not only to aficionados of fluorescence spectroscopy and its applications in biology, chemistry and physics, but also to those with a general interest in the historical development of an important scientific field.
Author : David M. Jameson
Publisher : Springer Science & Business Media
ISBN 13 : 1468456199
Total Pages : 459 pages
Book Rating : 4.4/5 (684 download)
Download or read book Fluorescent Biomolecules written by David M. Jameson and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 459 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume is based on an international symposium held during September 9-12, 1986 in Bocca di Magra, Italy. The intent of the organizers was to bring together expert practitioners of fluorescence spectroscopy, particularly as applied to biological systems, to assess recent developments in the field and discuss future directions. At the same time the meeting was intended to honor the singular and outstanding scientific career of Gregorio Weber on the occasion of his seventieth birthday. Gregorio Weber is truly the pioneer in the application of fluores cence methods to biochemistry and biophysics. A complete list of his scientific contributions to fluorescence and to protein biochemistry is beyond the scope of this preface. Suffice it to say that since his initial landmark articles on fluorescence, published in the late 1940's and early 1950's, Gregorio Weber has continued to make seminal contribu tions to both the theory and practice of fluorescence and, contrary to many who might be tempted to rest on their laurels, he shows no signs of slackening his pace. In addition to his more obvious tangible contributions to the scientific field, Gregorio Weber has made equally valuable contributions of another type. Specifically, he has had the most profound impact, both profeSSionally and personally, on generations of young scientists.
Author : Benjamin F. Cravatt
Publisher : Springer
ISBN 13 : 3030111431
Total Pages : 417 pages
Book Rating : 4.0/5 (31 download)
Download or read book Activity-Based Protein Profiling written by Benjamin F. Cravatt and published by Springer. This book was released on 2019-01-25 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.
Author : Chris D. Geddes
Publisher : Springer Science & Business Media
ISBN 13 : 0387236473
Total Pages : 313 pages
Book Rating : 4.3/5 (872 download)
Download or read book Advanced Concepts in Fluorescence Sensing written by Chris D. Geddes and published by Springer Science & Business Media. This book was released on 2010-07-18 with total page 313 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over the last decade, fluorescence has become the dominant tool in biotechnology and medical imaging. These exciting advances have been underpinned by the advances in time-resolved techniques and instrumentation, probe design, chemical / biochemical sensing, coupled with our furthered knowledge in biology. Complementary volumes 9 & 10, Advanced Concepts of Fluorescence Sensing: Small Molecule Sensing and Advanced Concepts of Fluorescence Sensing: Macromolecular Sensing, aim to summarize the current state of the art in fluorescent sensing. For this reason, Drs. Geddes and Lakowicz have invited chapters, encompassing a broad range of fluorescence sensing techniques. Some chapters deal with small molecule sensors, such as for anions, cations, and CO2, while others summarize recent advances in protein-based and macromolecular sensors. The Editors have, however, not included DNA or RNA based sensing in this volume, as this were reviewed in Volume 7 and is to be the subject of a more detailed volume in the near future.
Author : Bernard Valeur
Publisher : John Wiley & Sons
ISBN 13 : 3527650024
Total Pages : 595 pages
Book Rating : 4.5/5 (276 download)
Download or read book Molecular Fluorescence written by Bernard Valeur and published by John Wiley & Sons. This book was released on 2013-03-27 with total page 595 pages. Available in PDF, EPUB and Kindle. Book excerpt: Molecular Fluorescence This second edition of the well-established bestseller is completely updated and revised with approximately 30 % additional material, including two new chapters on applications, which has seen the most significant developments. The comprehensive overview written at an introductory level covers fundamental aspects, principles of instrumentation and practical applications, while providing many valuable tips. For photochemists and photophysicists, physical chemists, molecular physicists, biophysicists, biochemists and biologists, lecturers and students of chemistry, physics, and biology.
Author : Stephen G Schulman
Publisher : Elsevier
ISBN 13 : 1483160998
Total Pages : 299 pages
Book Rating : 4.4/5 (831 download)
Download or read book Fluorescence and Phosphorescence Spectroscopy written by Stephen G Schulman and published by Elsevier. This book was released on 2017-05-17 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: Fluorescence and Phosphorescence Spectroscopy: Physicochemical Principles and Practice deals with the physicochemical principles and applications of fluorescence and phosphorescence spectroscopy in experimental biology and chemistry. Topics covered include the absorption of light by molecules; instrumentation for the measurement of fluorescence and phosphorescence; solvent and acidity effects on electronic spectra; and polarization of fluorescence and phosphorescence. Comprised of four chapters, this book begins with a discussion on photophysical processes in isolated molecules and molecules in solution, paying particular attention to thermal equilibration of electronically excited molecules, phototautomerism, and coordination by metal ions. The next chapter describes the instrumentation for measuring fluorescence and phosphorescence, which consists essentially of a light source to electronically excite the sample; a monochromator to separate the light of desired energy from the source; a sample compartment; a second monochromator to isolate the sample's fluorescence energy from the excitation energy; a photodetector to translate the fluorescent light into an electrical signal; and a readout system such as a galvanometer or a recorder, coupled with an amplifier to determine the intensity of fluorescent light that is emitted. The final chapter is devoted to various applications of fluorescence and phosphorescence spectroscopy, including the analysis of organic and inorganic compounds. This monograph is written primarily for analytical chemists and biological scientists.
Author : George G. Guilbault
Publisher : CRC Press
ISBN 13 : 1000104443
Total Pages : 825 pages
Book Rating : 4.0/5 (1 download)
Download or read book Practical Fluorescence, Second Edition written by George G. Guilbault and published by CRC Press. This book was released on 2020-08-26 with total page 825 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a new edition (first, 1973) of an introduction to the principles and applications of all phases of luminescence spectroscopy. Contains (all rewritten) chapters on general aspects of luminescence, instrumentation, effects of molecular structure and environment, inorganic analysis and phosphorescence. The second edition also introduces new topics such as process, applications, bioprocess monitoring and biotechnology methods, soild surface luminescence and pesticide analysis, providing expanded coverage on chemiluminescence and environmental analysis and updates information on equipment, supplies newer references and more.
