Coherent X-ray diffractive imaging on the single-cell-level of microbial samples

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Publisher : Göttingen University Press
ISBN 13 : 3863951905
Total Pages : 254 pages
Book Rating : 4.8/5 (639 download)

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Book Synopsis Coherent X-ray diffractive imaging on the single-cell-level of microbial samples by : Robin Niklas Wilke

Download or read book Coherent X-ray diffractive imaging on the single-cell-level of microbial samples written by Robin Niklas Wilke and published by Göttingen University Press. This book was released on 2015 with total page 254 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since its first experimental demonstration in 1999, Coherent X-Ray Diffractive Imaging has become one of the most promising high resolution X-Ray imaging techniques using coherent radiation produced by brilliant synchrotron storage rings. The ability to directly invert diffraction data with the help of advanced algorithms has paved the way for microscopic investigations and wave-field analyses on the spatial scale of nanometres without the need for inefficient imaging lenses. X-Ray phase contrast which is a measure of the electron density is an important contrast mode of soft biological specimens. For the case of many dominant elements of soft biological matter, the electron density can be converted into an effective mass density offering a unique quantitative information channel which may shed light on important questions such as DNA compaction in the bacterial nucleoid through ‚weighing with light‘. In this work X-Ray phase contrast maps have been obtained from different biological samples by exploring different methods. In particular, the techniques Ptychography and Waveguide-Holographic-Imaging have been used to obtain twodimensional and three-dimensional mass density maps on the single-cell-level of freeze-dried cells of the bacteria Deinococcus radiodurans, Bacillus subtilis and Bacillus thuringiensis allowing, for instance, to estimate the dry weight of the bacterial genome in a near native state. On top of this, reciprocal space information from coherent small angle X-Ray scattering (cellular Nano-Diffraction) of the fine structure of the bacterial cells has been recorded in a synergistic manner and has been analysed down to a resolution of about 2.3/nm exceeding current limits of direct imaging approaches. Furthermore, the dynamic range of present detector technology being one of the major limiting factors of ptychographic phasing of farfield diffraction data has been significantly increased. Overcoming this problem for the case of the very intense X-Ray beam produced by Kirkpatrick-Baez mirrors has been explored by using semi-transparent central stops.

X-Ray Diffraction

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Publisher : CRC Press
ISBN 13 : 9814303607
Total Pages : 438 pages
Book Rating : 4.8/5 (143 download)

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Book Synopsis X-Ray Diffraction by : Oliver H. Seeck

Download or read book X-Ray Diffraction written by Oliver H. Seeck and published by CRC Press. This book was released on 2015-02-10 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-resolution x-ray diffraction and scattering is a key tool for structure analysis not only in bulk materials but also at surfaces and buried interfaces from the sub-nanometer range to micrometers. This book offers an overview of diffraction and scattering methods currently available at modern synchrotron sources and illustrates bulk and interface investigations of solid and liquid matter with up-to-date research examples. It presents important characteristics of the sources, experimental set-up, and new detector developments. The book also considers future exploitation of x-ray free electron lasers for diffraction applications.

Nanoscale Photonic Imaging

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Publisher : Springer Nature
ISBN 13 : 3030344134
Total Pages : 634 pages
Book Rating : 4.0/5 (33 download)

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Book Synopsis Nanoscale Photonic Imaging by : Tim Salditt

Download or read book Nanoscale Photonic Imaging written by Tim Salditt and published by Springer Nature. This book was released on 2020-06-09 with total page 634 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book, edited and authored by a team of world-leading researchers, provides a broad overview of advanced photonic methods for nanoscale visualization, as well as describing a range of fascinating in-depth studies. Introductory chapters cover the most relevant physics and basic methods that young researchers need to master in order to work effectively in the field of nanoscale photonic imaging, from physical first principles, to instrumentation, to mathematical foundations of imaging and data analysis. Subsequent chapters demonstrate how these cutting edge methods are applied to a variety of systems, including complex fluids and biomolecular systems, for visualizing their structure and dynamics, in space and on timescales extending over many orders of magnitude down to the femtosecond range. Progress in nanoscale photonic imaging in Göttingen has been the sum total of more than a decade of work by a wide range of scientists and mathematicians across disciplines, working together in a vibrant collaboration of a kind rarely matched. This volume presents the highlights of their research achievements and serves as a record of the unique and remarkable constellation of contributors, as well as looking ahead at the future prospects in this field. It will serve not only as a useful reference for experienced researchers but also as a valuable point of entry for newcomers.

Synchrotron Light Sources and Free-Electron Lasers

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Publisher : Springer
ISBN 13 : 9783319143934
Total Pages : 0 pages
Book Rating : 4.1/5 (439 download)

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Book Synopsis Synchrotron Light Sources and Free-Electron Lasers by : Eberhard J. Jaeschke

Download or read book Synchrotron Light Sources and Free-Electron Lasers written by Eberhard J. Jaeschke and published by Springer. This book was released on 2016-05-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hardly any other discovery of the nineteenth century did have such an impact on science and technology as Wilhelm Conrad Röntgen’s seminal find of the X-rays. X-ray tubes soon made their way as excellent instruments for numerous applications in medicine, biology, materials science and testing, chemistry and public security. Developing new radiation sources with higher brilliance and much extended spectral range resulted in stunning developments like the electron synchrotron and electron storage ring and the freeelectron laser. This handbook highlights these developments in fifty chapters. The reader is given not only an inside view of exciting science areas but also of design concepts for the most advanced light sources. The theory of synchrotron radiation and of the freeelectron laser, design examples and the technology basis are presented. The handbook presents advanced concepts like seeding and harmonic generation, the booming field of Terahertz radiation sources and upcoming brilliant light sources driven by laser-plasma accelerators. The applications of the most advanced light sources and the advent of nanobeams and fully coherent x-rays allow experiments from which scientists in the past could not even dream. Examples are the diffraction with nanometer resolution, imaging with a full 3D reconstruction of the object from a diffraction pattern, measuring the disorder in liquids with high spatial and temporal resolution. The 20th century was dedicated to the development and improvement of synchrotron light sources with an ever ongoing increase of brilliance. With ultrahigh brilliance sources, the 21st century will be the century of x-ray lasers and their applications. Thus, we are already close to the dream of condensed matter and biophysics: imaging single (macro)molecules and measuring their dynamics on the femtosecond timescale to produce movies with atomic resolution.

Coherent X-Ray Optics

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Publisher : Oxford University Press on Demand
ISBN 13 : 0198567286
Total Pages : 424 pages
Book Rating : 4.1/5 (985 download)

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Book Synopsis Coherent X-Ray Optics by : David Paganin

Download or read book Coherent X-Ray Optics written by David Paganin and published by Oxford University Press on Demand. This book was released on 2006-01-12 with total page 424 pages. Available in PDF, EPUB and Kindle. Book excerpt: X-ray optics is undergoing a renaissance, which may be paralleled to that experienced by visible-light optics following the invention of the laser. The associated surge of activity in "coherent" x-ray optics has been documented in this monograph, the first of its type in the field.

A Study on New Approaches in Coherent X-ray Microscopy of Biological Specimens

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Publisher : Universitätsverlag Göttingen
ISBN 13 : 3863950232
Total Pages : 226 pages
Book Rating : 4.8/5 (639 download)

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Book Synopsis A Study on New Approaches in Coherent X-ray Microscopy of Biological Specimens by : Klaus Giewekemeyer

Download or read book A Study on New Approaches in Coherent X-ray Microscopy of Biological Specimens written by Klaus Giewekemeyer and published by Universitätsverlag Göttingen. This book was released on 2011 with total page 226 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of coherent x rays for microscopic imaging has seen a rapid and ongoing development within the past decade, driven by an increasing availability of highly brilliant and coherent sources worldwide. Accordingly, novel methods have been developed, which replace the microscope‘s objective lens by a numerical reconstruction scheme. The aim of the present work is to study how very recent experimental and algorithmic developments in the field can be implemented towards a highly sensitive and fully quantitative microscopy method for imaging of biological cells. To this end, different experimental approaches are studied, based on coherent far-field as well as near-field diffraction. At first, an application of the novel ptychographic imaging method to single biological cells is presented. In particular, it is demonstrated how weakly scattering biological specimens can be imaged with fully quantitative density contrast. Alongside, a sueccessful extension of the method towards soft x-ray energies is described.In the second part of the work it is shown how x-ray waveguides can be used as a point source for propagation-based microscopy of single cells in the hard x-ray regime. The specifically devised iterative reconstruction scheme allows for full quantitativity and high sensitivity and thus enables an application to single biological cells. The work contains a thorough introduction into the x-ray optical methods applied and aims at a useful and self-contained overview on aspects of signal and Fourier theory relevant for the used numerical propagation schemes.

X-Ray Diffraction Imaging of Biological Cells

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Publisher : Springer
ISBN 13 : 443156618X
Total Pages : 243 pages
Book Rating : 4.4/5 (315 download)

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Book Synopsis X-Ray Diffraction Imaging of Biological Cells by : Masayoshi Nakasako

Download or read book X-Ray Diffraction Imaging of Biological Cells written by Masayoshi Nakasako and published by Springer. This book was released on 2018-03-29 with total page 243 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this book, the author describes the development of the experimental diffraction setup and structural analysis of non-crystalline particles from material science and biology. Recent advances in X-ray free electron laser (XFEL)-coherent X-ray diffraction imaging (CXDI) experiments allow for the structural analysis of non-crystalline particles to a resolution of 7 nm, and to a resolution of 20 nm for biological materials. Now XFEL-CXDI marks the dawn of a new era in structural analys of non-crystalline particles with dimensions larger than 100 nm, which was quite impossible in the 20th century. To conduct CXDI experiments in both synchrotron and XFEL facilities, the author has developed apparatuses, named KOTOBUKI-1 and TAKASAGO-6 for cryogenic diffraction experiments on frozen-hydrated non-crystalline particles at around 66 K. At the synchrotron facility, cryogenic diffraction experiments dramatically reduce radiation damage of specimen particles and allow tomography CXDI experiments. In addition, in XFEL experiments, non-crystalline particles scattered on thin support membranes and flash-cooled can be used to efficiently increase the rate of XFEL pulses. The rate, which depends on the number density of scattered particles and the size of X-ray beams, is currently 20-90%, probably the world record in XFEL-CXDI experiments. The experiment setups and results are introduced in this book. The author has also developed software suitable for efficiently processing of diffraction patterns and retrieving electron density maps of specimen particles based on the diffraction theory used in CXDI.

Medical Imaging Systems

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Publisher : Springer
ISBN 13 : 3319965204
Total Pages : 263 pages
Book Rating : 4.3/5 (199 download)

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Book Synopsis Medical Imaging Systems by : Andreas Maier

Download or read book Medical Imaging Systems written by Andreas Maier and published by Springer. This book was released on 2018-08-02 with total page 263 pages. Available in PDF, EPUB and Kindle. Book excerpt: This open access book gives a complete and comprehensive introduction to the fields of medical imaging systems, as designed for a broad range of applications. The authors of the book first explain the foundations of system theory and image processing, before highlighting several modalities in a dedicated chapter. The initial focus is on modalities that are closely related to traditional camera systems such as endoscopy and microscopy. This is followed by more complex image formation processes: magnetic resonance imaging, X-ray projection imaging, computed tomography, X-ray phase-contrast imaging, nuclear imaging, ultrasound, and optical coherence tomography.

Elements of Modern X-ray Physics

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Publisher : John Wiley & Sons
ISBN 13 : 0470973943
Total Pages : 440 pages
Book Rating : 4.4/5 (79 download)

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Book Synopsis Elements of Modern X-ray Physics by : Jens Als-Nielsen

Download or read book Elements of Modern X-ray Physics written by Jens Als-Nielsen and published by John Wiley & Sons. This book was released on 2011-04-04 with total page 440 pages. Available in PDF, EPUB and Kindle. Book excerpt: Eagerly awaited, this second edition of a best-selling text comprehensively describes from a modern perspective the basics of x-ray physics as well as the completely new opportunities offered by synchrotron radiation. Written by internationally acclaimed authors, the style of the book is to develop the basic physical principles without obscuring them with excessive mathematics. The second edition differs substantially from the first edition, with over 30% new material, including: A new chapter on non-crystalline diffraction - designed to appeal to the large community who study the structure of liquids, glasses, and most importantly polymers and bio-molecules A new chapter on x-ray imaging - developed in close cooperation with many of the leading experts in the field Two new chapters covering non-crystalline diffraction and imaging Many important changes to various sections in the book have been made with a view to improving the exposition Four-colour representation throughout the text to clarify key concepts Extensive problems after each chapter There is also supplementary book material for this title available online (http://booksupport.wiley.com). Praise for the previous edition: "The publication of Jens Als-Nielsen and Des McMorrow's Elements of Modern X-ray Physics is a defining moment in the field of synchrotron radiation... a welcome addition to the bookshelves of synchrotron–radiation professionals and students alike.... The text is now my personal choice for teaching x-ray physics...." —Physics Today, 2002

Colloidal Crystals of Spheres and Cubes in Real and Reciprocal Space

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Publisher : Springer
ISBN 13 : 3319148095
Total Pages : 164 pages
Book Rating : 4.3/5 (191 download)

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Book Synopsis Colloidal Crystals of Spheres and Cubes in Real and Reciprocal Space by : Janne-Mieke Meijer

Download or read book Colloidal Crystals of Spheres and Cubes in Real and Reciprocal Space written by Janne-Mieke Meijer and published by Springer. This book was released on 2015-06-11 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis presents an in-depth study on the effect of colloidal particle shape and formation mechanism on self-organization and the final crystal symmetries that can be achieved. It demonstrates how state-of-the-art X-ray diffraction techniques can be used to produce detailed characterizations of colloidal crystal structures prepared using different self-assembly techniques, and how smart systems can be used to investigate defect formation and diffusion in-situ. One of the most remarkable phenomena exhibited by concentrated suspensions of colloidal particles is the spontaneous self-organization into structures with long-range spatial and/or orientational orders. The study also reveals the subtle structural variations that arise by changing the particle shape from spherical to that of a rounded cube. In particular, the roundness of the cube corners, when combined with the self-organization pathway, convective assembly or sedimentation, was shown to influence the final crystal symmetries.

Polymer Science: A Comprehensive Reference

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Author :
Publisher : Newnes
ISBN 13 : 0080878628
Total Pages : 7752 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Polymer Science: A Comprehensive Reference by :

Download or read book Polymer Science: A Comprehensive Reference written by and published by Newnes. This book was released on 2012-12-05 with total page 7752 pages. Available in PDF, EPUB and Kindle. Book excerpt: The progress in polymer science is revealed in the chapters of Polymer Science: A Comprehensive Reference, Ten Volume Set. In Volume 1, this is reflected in the improved understanding of the properties of polymers in solution, in bulk and in confined situations such as in thin films. Volume 2 addresses new characterization techniques, such as high resolution optical microscopy, scanning probe microscopy and other procedures for surface and interface characterization. Volume 3 presents the great progress achieved in precise synthetic polymerization techniques for vinyl monomers to control macromolecular architecture: the development of metallocene and post-metallocene catalysis for olefin polymerization, new ionic polymerization procedures, and atom transfer radical polymerization, nitroxide mediated polymerization, and reversible addition-fragmentation chain transfer systems as the most often used controlled/living radical polymerization methods. Volume 4 is devoted to kinetics, mechanisms and applications of ring opening polymerization of heterocyclic monomers and cycloolefins (ROMP), as well as to various less common polymerization techniques. Polycondensation and non-chain polymerizations, including dendrimer synthesis and various "click" procedures, are covered in Volume 5. Volume 6 focuses on several aspects of controlled macromolecular architectures and soft nano-objects including hybrids and bioconjugates. Many of the achievements would have not been possible without new characterization techniques like AFM that allowed direct imaging of single molecules and nano-objects with a precision available only recently. An entirely new aspect in polymer science is based on the combination of bottom-up methods such as polymer synthesis and molecularly programmed self-assembly with top-down structuring such as lithography and surface templating, as presented in Volume 7. It encompasses polymer and nanoparticle assembly in bulk and under confined conditions or influenced by an external field, including thin films, inorganic-organic hybrids, or nanofibers. Volume 8 expands these concepts focusing on applications in advanced technologies, e.g. in electronic industry and centers on combination with top down approach and functional properties like conductivity. Another type of functionality that is of rapidly increasing importance in polymer science is introduced in volume 9. It deals with various aspects of polymers in biology and medicine, including the response of living cells and tissue to the contact with biofunctional particles and surfaces. The last volume is devoted to the scope and potential provided by environmentally benign and green polymers, as well as energy-related polymers. They discuss new technologies needed for a sustainable economy in our world of limited resources. Provides broad and in-depth coverage of all aspects of polymer science from synthesis/polymerization, properties, and characterization methods and techniques to nanostructures, sustainability and energy, and biomedical uses of polymers Provides a definitive source for those entering or researching in this area by integrating the multidisciplinary aspects of the science into one unique, up-to-date reference work Electronic version has complete cross-referencing and multi-media components Volume editors are world experts in their field (including a Nobel Prize winner)

Advanced Holography

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Publisher : BoD – Books on Demand
ISBN 13 : 9533077298
Total Pages : 392 pages
Book Rating : 4.5/5 (33 download)

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Book Synopsis Advanced Holography by : Izabela Naydenova

Download or read book Advanced Holography written by Izabela Naydenova and published by BoD – Books on Demand. This book was released on 2011-11-09 with total page 392 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Holography - Metrology and Imaging covers digital holographic microscopy and interferometry, including interferometry in the infra red. Other topics include synthetic imaging, the use of reflective spatial light modulators for writing dynamic holograms and image display using holographic screens. Holography is discussed as a vehicle for artistic expression and the use of software for the acquisition of skills in optics and holography is also presented. Each chapter provides a comprehensive introduction to a specific topic, with a survey of developments to date.

21st Century Nanoscience – A Handbook

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Publisher : CRC Press
ISBN 13 : 1000702863
Total Pages : 369 pages
Book Rating : 4.0/5 (7 download)

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Book Synopsis 21st Century Nanoscience – A Handbook by : Klaus D. Sattler

Download or read book 21st Century Nanoscience – A Handbook written by Klaus D. Sattler and published by CRC Press. This book was released on 2020-04-08 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This 21st Century Nanoscience Handbook will be the most comprehensive, up-to-date large reference work for the field of nanoscience. Handbook of Nanophysics by the same editor published in the fall of 2010 and was embraced as the first comprehensive reference to consider both fundamental and applied aspects of nanophysics. This follow-up project has been conceived as a necessary expansion and full update that considers the significant advances made in the field since 2010. It goes well beyond the physics as warranted by recent developments in the field. This seventh volume in a ten-volume set covers bioinspired systems and methods. Key Features: Provides the most comprehensive, up-to-date large reference work for the field. Chapters written by international experts in the field. Emphasises presentation and real results and applications. This handbook distinguishes itself from other works by its breadth of coverage, readability and timely topics. The intended readership is very broad, from students and instructors to engineers, physicists, chemists, biologists, biomedical researchers, industry professionals, governmental scientists, and others whose work is impacted by nanotechnology. It will be an indispensable resource in academic, government, and industry libraries worldwide. The fields impacted by nanophysics extend from materials science and engineering to biotechnology, biomedical engineering, medicine, electrical engineering, pharmaceutical science, computer technology, aerospace engineering, mechanical engineering, food science, and beyond.

Dynamical Theory of X-ray Diffraction

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Publisher : Oxford University Press, USA
ISBN 13 : 9780198528920
Total Pages : 700 pages
Book Rating : 4.5/5 (289 download)

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Book Synopsis Dynamical Theory of X-ray Diffraction by : André Authier

Download or read book Dynamical Theory of X-ray Diffraction written by André Authier and published by Oxford University Press, USA. This book was released on 2004 with total page 700 pages. Available in PDF, EPUB and Kindle. Book excerpt: Publisher Description

In-situ Materials Characterization

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Publisher : Springer Science & Business Media
ISBN 13 : 3642451527
Total Pages : 265 pages
Book Rating : 4.6/5 (424 download)

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Book Synopsis In-situ Materials Characterization by : Alexander Ziegler

Download or read book In-situ Materials Characterization written by Alexander Ziegler and published by Springer Science & Business Media. This book was released on 2014-04-01 with total page 265 pages. Available in PDF, EPUB and Kindle. Book excerpt: The behavior of nanoscale materials can change rapidly with time either because the environment changes rapidly or because the influence of the environment propagates quickly across the intrinsically small dimensions of nanoscale materials. Extremely fast time resolution studies using X-rays, electrons and neutrons are of very high interest to many researchers and is a fast-evolving and interesting field for the study of dynamic processes. Therefore, in situ structural characterization and measurements of structure-property relationships covering several decades of length and time scales (from atoms to millimeters and femtoseconds to hours) with high spatial and temporal resolutions are crucially important to understand the synthesis and behavior of multidimensional materials. The techniques described in this book will permit access to the real-time dynamics of materials, surface processes and chemical and biological reactions at various time scales. This book provides an interdisciplinary reference for research using in situ techniques to capture the real-time structural and property responses of materials to surrounding fields using electron, optical and x-ray microscopies (e.g. scanning, transmission and low-energy electron microscopy and scanning probe microscopy) or in the scattering realm with x-ray, neutron and electron diffraction.

X-ray Standing Wave Technique, The: Principles And Applications

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Publisher : World Scientific
ISBN 13 : 9814513105
Total Pages : 557 pages
Book Rating : 4.8/5 (145 download)

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Book Synopsis X-ray Standing Wave Technique, The: Principles And Applications by : Jorg Zegenhagen

Download or read book X-ray Standing Wave Technique, The: Principles And Applications written by Jorg Zegenhagen and published by World Scientific. This book was released on 2013-01-30 with total page 557 pages. Available in PDF, EPUB and Kindle. Book excerpt: The X-ray standing wave (XSW) technique is an X-ray interferometric method combining diffraction with a multitude of spectroscopic techniques. It is extremely powerful for obtaining information about virtually all properties of surfaces and interfaces on the atomic scale. However, as with any other technique, it has strengths and limitations. The proper use and necessary understanding of this method requires knowledge in quite different fields of physics and technology. This volume presents comprehensively the theoretical background, technical requirements and distinguished experimental highlights of the technique. Containing contributions from the most prominent experts of the technique, such as Andre Authier, Boris Batterman, Michael J Bedzyk, Jene Golovchenko, Victor Kohn, Michail Kovalchuk, Gerhard Materlik and D Phil Woodruff, the book equips scientists with all the necessary information and knowledge to understand and use the XSW technique in practically all applications.

Optimization of Waveguide Optics for Lensless X-ray Imaging

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Publisher : Universitätsverlag Göttingen
ISBN 13 : 3863950151
Total Pages : 169 pages
Book Rating : 4.8/5 (639 download)

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Book Synopsis Optimization of Waveguide Optics for Lensless X-ray Imaging by : Sven Philip Krüger

Download or read book Optimization of Waveguide Optics for Lensless X-ray Imaging written by Sven Philip Krüger and published by Universitätsverlag Göttingen. This book was released on 2011 with total page 169 pages. Available in PDF, EPUB and Kindle. Book excerpt: Lensless x-ray imaging is a promising method to determine the three-dimensional structure of material science and biological specimens at the nanoscale. The development of this technique is strongly related to the optimization of x-ray optics since the image formation and object reconstruction depend significantly on the properties of the illumination wave-field. Waveguide optics act as quasi-point sources and enable the spatial and coherent filtering of x-ray beams. Up to now, x-ray waveguides were severely limited in transmission and flux, restricting their use to high-contrast test structures with moderate resolution and long accumulation times. To overcome these limitations, a novel waveguide design with an optimized refractive index profile is presented which significantly minimizes the absorption of the modes propagating inside the waveguide. Experimental results along with simulations show that these two-component planar x-ray waveguides provide small beam cross-sections along with a high photon flux at the exit. By a serial arrangement of two waveguide slices an optimized illumination source has been developed for high-resolution microscopy, as demonstrated in proof-of-concept imaging experiments.