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Author : Anna Catherine Shawyer
Publisher :
ISBN 13 :
Total Pages : 164 pages
Book Rating : 4.:/5 (516 download)
Download or read book Estrogen Mediated Cell Proliferation in MCF-7 BUS Human Breast Cancer Cells written by Anna Catherine Shawyer and published by . This book was released on 2002 with total page 164 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Jenifer Robyn Prosperi
Publisher :
ISBN 13 :
Total Pages : 238 pages
Book Rating : 4.:/5 (123 download)
Download or read book In Vitro and in Vivo Characterization of the Estrogen Dependent Human Breast Cancer Cell Line, MCF-7, Over-expressing Cyclooxygenase-2 written by Jenifer Robyn Prosperi and published by . This book was released on 2006 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Abstract: The hypothesis for this dissertation is that cyclooxygenase-2 (Cox-2) and its product prostaglandin E2 (PGE2) directly regulate proliferation, invasion, angiogenesis, aromatase transcription, and estradiol production in human breast tumor cells in vitro and in vivo. To investigate the regulatory role of Cox-2, human Cox-2 cDNA was transfected into the Cox-2 null, estrogen-dependent, slow-growing, non-metastatic MCF-7 human breast tumor cells. Two transfectant clones, MCF-7/Cox-2 Clone 8 and MCF-7/Cox-2 Clone 10, and a negative control transfected with the empty vector, MCF-7/vector control, were used for further studies. In vitro studies demonstrated that the presence of Cox-2 cDNA decreased the doubling times of MCF-7/Cox-2 Clones 8 and 10, increased in vitro invasive characteristics, and increased splice variant specific mRNA expression of vascular endothelial growth factor (VEGF). In addition, total and promoter-specific mRNA expression of aromatase was elevated, with a subsequent increase in production of 17 beta-estradiol. MCF-7/Cox-2 Clone 10 human breast tumor cells also had increased levels of two PGE2 receptors, EP1 and EP2, compared to MCF-7/vector control. EP1 and/or EP2 stimulation enhanced proliferation, splice variant specific VEGF expression, and aromatase expression through the newly identified promoter pI. 7. Injection of 5 x 10^6 MCF-7/Cox-2 Clone 10 human breast tumor cells into non-ovariectomized or ovariectomized Crl:Nu-Foxn1^nu mice resulted in rapid formation of large, invasive, angiogenic tumors, with a 3-fold increase in tumor volume compared to mice injected with MCF-7/vector control cells. 17 beta-estradiol pellet implantation increased the tumor volume formed in ovariectomized Crl:Nu-Foxn1^nu mice compared to the tumor volume of MCF-7/Cox-2 Clone 10 human breast tumor xenografts in non-ovariectomized Crl:Nu-Foxn1^nu mice, suggesting that exogenous estrogen was a factor in determining volume of the human breast tumors. This model of Cox-2 mediated human breast tumorigenesis provides a unique system in which to study the molecular changes associated with the presence of Cox-2 in estrogen-dependent human breast cancer. This model can be used to identify novel chemotherapeutic and chemopreventive targets, and can be used to evaluate agents that target Cox-2, PGE2, aromatase, estradiol, and the Her-2/neu oncogene, which have all been demonstrated as significant factors in human breast tumorigenesis.
Author : Fritz F. Parl
Publisher : IOS Press
ISBN 13 : 9780967335544
Total Pages : 280 pages
Book Rating : 4.3/5 (355 download)
Download or read book Estrogens, Estrogen Receptor and Breast Cancer written by Fritz F. Parl and published by IOS Press. This book was released on 2000 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: Estrogens have been implicated to play a role in the development of breast cancer. The purpose of this book is to provide a comprehensive analysis of experimental, clinical and epidemiological evidence in support of the carcinogenicity of estrogens.
Author : Malathy Shanmugam
Publisher :
ISBN 13 :
Total Pages : 284 pages
Book Rating : 4.3/5 (555 download)
Download or read book Regulation of the Protein Kinase C Delta Isoform by Estrogen in the MCF-7 Human Breast Cancer Cell Line and a Role for Protein Kinase C Delta in Growth Regulation written by Malathy Shanmugam and published by . This book was released on 1997 with total page 284 pages. Available in PDF, EPUB and Kindle. Book excerpt: Previous evidence in our laboratory demonstrated the ability of estrogen to regulate specifically the protein kinase C (PKC) $\delta$ isoform in the rat and rabbit corpus luteum. The purpose of this study was to evaluate estrogen's ability to regulate PKC, specifically the PKC $\delta$ isoform, in the estrogen responsive, MCF-7 human breast cancer cell line. With the increasingly characterized role of PKC $\delta$ in growth regulation, we were interested to also determine the ability of PKC $\delta$ to regulate growth in the MCF-7 cell line. Treatment of MCF-7 cells with 10$\sp{-10}$ through 10$\sp{-8}$ M estrogen for 7 days down-regulated specifically PKC $\delta$ mRNA and protein; expression of other PKC isoforms was unchanged. These concentrations of estrogen were maximally proliferative for the MCF-7 cells and an inverse correlation between PKC $\delta$ expression and proliferation in MCF-7 cells was observed. PKC $\delta$ protein levels were found to be 10 fold higher in MCF-7 compared to the estrogen receptor-negative, MDA-MB 231 cells which grow aggressively in the absence of estrogen. In view of the inverse correlation between PKC $\delta$ expression and proliferation in MCF-7 cells we wished to determine whether activation of PKC $\delta$ could signal growth inhibition. Phorbol ester treatments that lead to G1 growth arrest and induction of the cyclin-dependent kinase inhibitor p21$\rm\sp{Waf1/Cip1}$ in MCF-7 cells promoted activation of PKC $\delta$, as evidenced in immunecomplex phosphorylation assays. To show that phorbol ester stimulated G1 growth arrest could be mediated by the induction of p21$\rm\sp{Waf1/Cip1}$ by activated PKC $\delta,$ we demonstrated that constitutively active PKC $\delta$ in the absence but not in the presence of dominant negative PKC $\delta$ activated the WAF1/CIP1 promoter-luciferase construct in transient transfection assays. Activated PKC $\delta$ can therefore signal the transcriptional induction of p21$\rm\sp{WAF1/CIP1}.$ Our results are consistent with the hypothesis that modulation of PKC $\delta$ expression and activation state provides a pathway for growth regulation in breast cancer cells.
Author : Kyuri Kim
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (774 download)
Download or read book Regulation of Estrogen Receptor-alpha Mediated Gene Expression and Endocrine Resistance Through Estrogen Receptor-alpha Phosphorylation and Micro-RNA in Breast Cancer written by Kyuri Kim and published by . This book was released on 2011 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Estrogens are associated with the development and progression of breast cancer in addition to their role in normal reproductive physiology, and estrogen receptors (ER) mediate the actions of estrogen in target tissues by regulating the expression of numerous biologically important target genes. The progression of human breast cancer and the development of resistance to endocrine therapies are thought to be associated with ER phosphorylation. We generated multiple combinations of ER phospho-mutants, at residues serine 104, 106, 118, 167, 236, and 305, and examined their impact on receptor half-life, the agonist and antagonist balance of selective estrogen receptor modulators (SERMs) and selective estrogen receptor downregulators (SERDs), the regulation of ER transcriptional activity, and stimulation of cell proliferation in response to estradiol and SERMs/SERD. We showed that changes in ER affecting the phosphorylation status of the receptor greatly impact receptor function and differential SERM and SERD modulated cellular responses that could contribute to resistance to endocrine therapies in breast cancer. We also studied the regulation of microRNAs (miRNAs) by estradiol and growth factors through ER and extracellular signal-regulated kinase 2 (ERK2) in order to understand their physiological impact on breast cancer. We identified nine miRNA- encoding genes harboring overlapping ER and ERK2 binding sites close to their transcription start sites, which require ER and ERK2 for transcriptional induction as well as estradiol- mediated miRNA regulation. We then identified TP63, a target of miR-101, miR-190 and miR- 196a2, and showed that TP63 plays an important role in estradiol- or growth factor-mediated cellular response in breast cancer cells (MCF-7 and MDA-MB-231) by increasing tumor cell growth and in vitro invasion mainly controlled by miR-196a2 action. These results suggest a tumor-suppressive role of miR-196a2 in regulating TP63 expression and the aggressive behavior of breast cancers.
Author : Shyam Narayan Sundar
Publisher :
ISBN 13 :
Total Pages : 438 pages
Book Rating : 4.:/5 (35 download)
Download or read book Phytochemical Regulation of Estrogen Responsiveness and Proliferation of Human Breast Cancer Cells written by Shyam Narayan Sundar and published by . This book was released on 2006 with total page 438 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Virgil Craig Jordan
Publisher : Univ of Wisconsin Press
ISBN 13 : 9780299104801
Total Pages : 564 pages
Book Rating : 4.1/5 (48 download)
Download or read book Estrogen/antiestrogen Action and Breast Cancer Therapy written by Virgil Craig Jordan and published by Univ of Wisconsin Press. This book was released on 1986 with total page 564 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book marks one of the achievements of the "Estrogen and Antiestrogen Action: Basic and Clinical Aspects" Wisconsin Clinical Cancer Center conference in June 1984. It is not intended to be a recount of the meeting proceedings but is, rather, a historical review of the development of this field of endeavor during the past 30 years. The chapters have been written by many of the leading experts in the field who were asked to recount the development of a particular area or laboratory idea in which they had been personally involved. The book is intended to provide both scientists and clinicians with a single-volume overview of both the basic principles of hormonal control of breast cancer and the recent clinical results from cooperative groups around the world.
Author : Michael Tsung-Ju Yang
Publisher :
ISBN 13 :
Total Pages : 136 pages
Book Rating : 4.:/5 (59 download)
Download or read book Role of Estrogen Receptors in Estrogen and Xenoestrogens-induced Breast Cancer Cell Proliferation written by Michael Tsung-Ju Yang and published by . This book was released on 1998 with total page 136 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Marc E. Lippman
Publisher : Springer Science & Business Media
ISBN 13 : 1461539404
Total Pages : 455 pages
Book Rating : 4.4/5 (615 download)
Download or read book Regulatory Mechanisms in Breast Cancer written by Marc E. Lippman and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 455 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Breast Cancer: Cellular and Molecular Biology [Kluwer Academic Pub lishers, 1988], we tried to present an introduction to the emerging basic studies on steroid receptors, oncogenes, and growth factors in the regulation of normal and malignant mammary epithelium. The response to this volume was superb, indicating a tremendous interest in basic growth regulatory mechanisms governing breast cancer and controlling its malignant progres sion. In the two years since its publication, much new and exciting in formation has been published and the full interplay of regulatory mechanisms is now beginning to emerge. We have divided this book into four sections that we hope will unify important concepts and help to crystallize areas of consensus and/or disagreement among a diverse group of basic and clinical scientists working on the disease. The first section is devoted to studies on oncogenes, antioncogenes, proliferation, and tumor prognosis. The first chapter, by Sunderland and McGuire, introduces the characteristics of breast cancer as studied by patho logists to establish prognostic outcome. Of particular interest is a new proto oncogene called HER-2 (or neu), which is rapidly becoming accepted as a valuable new tumor marker of poor prognosis. The second chapter, by Lee Bookstein and Lee, introduces the best known antioncogene, the retinoblas toma antioncogene, whose expression is sometimes lost in breast cancer. Malignant progression appears to be influenced by the balance of proto oncogene and antioncogene expression.
Author : Constance Clark Reese
Publisher :
ISBN 13 :
Total Pages : 388 pages
Book Rating : 4.:/5 (49 download)
Download or read book Estradiol Stimulation and Androgen Inhibiton of the Growth of Human Breast Cancer Cell Lines written by Constance Clark Reese and published by . This book was released on 1989 with total page 388 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Ethel M. Cormier
Publisher :
ISBN 13 :
Total Pages : 488 pages
Book Rating : 4.:/5 (89 download)
Download or read book Development of Estrogen Independence and Antiestrogen Resistance in Breast Cancer Cells in Culture written by Ethel M. Cormier and published by . This book was released on 1988 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Catherine Sue Murphy
Publisher :
ISBN 13 :
Total Pages : 358 pages
Book Rating : 4.:/5 (89 download)
Download or read book Control of Breast Cancer Cell Replication in Vitro by Estrogens and Antiestrogens written by Catherine Sue Murphy and published by . This book was released on 1990 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Shun-Yuan Jiang
Publisher :
ISBN 13 :
Total Pages : 346 pages
Book Rating : 4.:/5 (89 download)
Download or read book Estrogen and Antiestrogen Regulation of Breast Cancer Cell Growth written by Shun-Yuan Jiang and published by . This book was released on 1991 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Dave Deonarine
Publisher :
ISBN 13 :
Total Pages : 0 pages
Book Rating : 4.:/5 (872 download)
Download or read book Mechanisms of Retinoic Acid-mediated Inhibition of Estrogen-induced Transcription and Growth in Human Breast Carcinoma Cells written by Dave Deonarine and published by . This book was released on 1996 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Retinoids have been shown to inhibit the growth of estrogen receptor-positive human breast cancer cells. In this study, it is shown that all-trans-retinoic acid (RA) inhibits the growth of MCF-7 cells by 60%. In these cells, we also found that RA inhibited estrogen-induced transcription. In transient transfection experiments using the vitellogenin-estrogen response element-CAT reporter construct, RA inhibited estrogen-induced transcription by 55%. Cotransfection of wildtype RAR$\alpha$ resulted in a 75% inhibition of estrogen-induced transcription. In order to investigate the mechanisms involved in RA-mediated inhibition of transcription, deletion mutants of RAR$\alpha$ were constructed. Point mutants of RAR$\alpha$ were also evaluated to determine their effects on RA-mediated inhibition of estrogen-induced transcription. Cotransfection of RAR$\alpha$ deletion mutants terminating after amino acid 414 resulted in wildtype inhibition. Using mutants terminating before amino acid 412, the inhibition was significantly reduced. RAR$\alpha$ mutants lacking amino acids 413 and 414 also showed significantly decreased inhibition. The amino acids 413 and 414 correspond to the activating function-2 (AF-2) region in the C-terminus of the RAR$\alpha.$ These results suggest that the RA-mediated inhibition of estrogen-induced transcription in human breast carcinoma cells is mediated by the AF-2 region of the RAR$\alpha.$ MCF-7 cells, stably transfected with a dominant negative RAR$\alpha, $ are growth inhibited by only 25% compared to 60% for untransfected or mock-transfected cells. Taken together, these results suggest that RA-mediated inhibition of estrogen-induced transcription is mediated by the AF-2 region of the RAR$\alpha$ and may play a role in RA-induced inhibition of estrogen receptor-positive human breast cancer cell growth.
Author : Chuanfeng Wang
Publisher :
ISBN 13 :
Total Pages : 420 pages
Book Rating : 4.:/5 (319 download)
Download or read book Effects of Phytoestrogens on Estrogen Synthesis in Preadipocytes and DNA Synthesis in Human Breast Cancer Cells written by Chuanfeng Wang and published by . This book was released on 1998 with total page 420 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author : Lei Chen
Publisher :
ISBN 13 :
Total Pages : 138 pages
Book Rating : 4.3/5 ( download)
Download or read book Alterations in Estrogen Receptor Signaling Pathways in Breast Cancer Cells with Acquired Antiestrogen Resistance written by Lei Chen and published by . This book was released on 2005 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :
Publisher :
ISBN 13 :
Total Pages : 37 pages
Book Rating : 4.:/5 (644 download)
Download or read book Membrane Estrogen Receptor Alpha Targeting and Its Association With SHC in Regulating Breast Cancer Cell Proliferation written by and published by . This book was released on 2003 with total page 37 pages. Available in PDF, EPUB and Kindle. Book excerpt: 17BETA-ESTRADIOL (E2) induces rapid, non-genomic effect in MCF-7 breast cancer cells, including rapid activation of MAPK, phosphorylation of adapter protein Shc, increase of the interaction between Shc and estrogen receptor (ERalpha). More strikingly E2 also induced a rapid membrane association of ERalpha: (1) Further studying the structure and function of ERalpha, we demonstrated that only membrane-associated ERalpha, but not cytosol and nuclear ones, mediates E2 effect on MAPK activation. (2) To study the mechanism of ERalpha membrane association, we further investigated the role of Shc in estrogen action. Here we demonstrated that in MCF-7 cells, Shc acts as a chaperon linking ERalpha to the cell membrane by binding to a transmembrane receptor IGF-1R. Further study is under the way to investigate the molecular mechanism among these protein complex formation and their biological effects in estrogen non-genomic action.
