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Engineering Yeast Strains To Enhance Bioethanol Production Efficiency
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Book Synopsis Engineering Yeast Strains to Enhance Bioethanol Production Efficiency by :
Download or read book Engineering Yeast Strains to Enhance Bioethanol Production Efficiency written by and published by . This book was released on 2008 with total page 116 pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has witnessed a tremendous increase in the production and demand for bio-fuels in the United States and worldwide. Bio-ethanol and bio-diesel are the two most common types of bio-fuels considered as potential substitutes to fossil oilbased fuel. In the U.S., maize seed is used as the main source of carbohydrate to produce bio-ethanol. The fermentation step is the most critical step in the production of bio-ethanol. The budding yeast Saccharomyces cerevisiae has generally been adopted as the main organism used to ferment maize seed for bio-ethanol production. One of the proposed strategies to improve and optimize bio-ethanol production is to develop new strains of S. cerevisiae with tolerance to stresses. The main objectives here were; generate S. cerevisiae mutants tolerant to high alcohol concentrations; test for their ability to ferment starch and partially characterize the mutations responsible for the new phenotypes. The chemical mutagen ethyl methane sulfonate (EMS) was used to mutagenize two strains of S. cerevisiae. The mutagenized yeast strains were exposed to high concentrations of ethanol and the tolerant mutants were selected. Mutant strains were sent to National Corn to Ethanol Research Center (NCERC) located in Edwardsville, Illinois to test for their fermentation ability and ethanol production under conditions similar to those present in ethanol production plants. The AFLP (Amplified Fragment Length Polymorphism) was used to identify some of the genes mutated in the tolerant strains. Enzyme assays were used to examine the effects of the mutations on the ethanol metabolism pathways. The research lead to the identification of four altered genes (CAN1, SLS1, VTH1 and COX6) in yeast strains with improved efficiency in ethanol production. The ADH (alcohol dehydrogenase) assay, redox reaction, showed that there was no difference in ADH activity between controls and mutants in either strain. Therefore, the mutations did not appear to affect primary ethanol metabolism. The improved mutant strains might impact the industry directly and/or help increase understanding of the complex mechanisms that regulate the tolerance of budding yeast to stresses encountered during fermentation.
Book Synopsis Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass by : Arindam Kuila
Download or read book Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass written by Arindam Kuila and published by Elsevier. This book was released on 2020-02-19 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Genetic and Metabolic Engineering for Improved Biofuel Production from Lignocellulosic Biomass describes the different aspects of biofuel production from lignocellulosic biomass. Each chapter presents different technological approaches for cost effective liquid biofuel production from agroresidues/biomass. Two chapters cover future direction and the possibilities of biomass-based biofuel production at the industrial level. The book provides a genetic and metabolic engineering approach for improved cellulase production and the potential of strains that can ferment both pentose and hexose sugars. The book also gives direction on how to overcome challenges for the further advancement of lignocellulosic biomass-based biofuel production. - Covers genetic engineering approaches for higher cellulase production from fungi - Includes genetic and metabolic engineering approaches for development of potential pentose and hexose fermenting strain which can tolerate high ethanol and toxic phenolic compounds - Describe different bioreactors used in different steps of biomass-based biofuel production - Outlines future prospects and potential of biofuel production from lignocellulosic biomass
Book Synopsis Systematic and Combinatorial Approaches for Metabolic Engineering of Optimal Yeast Strains to Produce Fuels and Chemicals by :
Download or read book Systematic and Combinatorial Approaches for Metabolic Engineering of Optimal Yeast Strains to Produce Fuels and Chemicals written by and published by . This book was released on 2014 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Xylanolytic Enzymes by : Pratima Bajpai
Download or read book Xylanolytic Enzymes written by Pratima Bajpai and published by Academic Press. This book was released on 2014-02-03 with total page 121 pages. Available in PDF, EPUB and Kindle. Book excerpt: Xylanolytic Enzymes describes the enzyme structure and its interaction with plant cell walls, the properties and production of different enzymes and their application, and the knowledge gathered on the hydrolysis mechanism of hemicellulose. The knowledge gathered about the hydrolysis mechanism of the hemicelluloses, especially xylans, has greatly promoted the rapid application of these enzymes in new areas. Recently there has been much industrial interest in xylan and its hydrolytic enzymatic complex, as a supplement and for the manufacturing of food, drinks, textiles, pulps and paper, and ethanol; and in xylitol production as a fermentation substrate for the production of enzymes. This book describes xylan as a major component of plant hemicelluloses. - Presents a thorough overview of all aspects of xylanolytic enzymes - Gives up-to-date authoritative information and cites pertinent research - Includes studies on xylanase regulation and synergistic action between multiple forms of xylanase
Book Synopsis Advances of Basic Science for Second Generation Bioethanol from Sugarcane by : Marcos S. Buckeridge
Download or read book Advances of Basic Science for Second Generation Bioethanol from Sugarcane written by Marcos S. Buckeridge and published by Springer. This book was released on 2017-03-10 with total page 217 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on the basic science recently produced in Brazil for the improvement of sugarcane as a bioenergy crop and as a raw material for 2nd generation bioethanol production. It reports achievements that have been advancing the science of cell walls, enzymes, genetics, and sustainability related to sugarcane technologies and give continuity to the research reported in the “Routes to Cellulosic Ethanol”, from Springer. The Introduction (Chapter I) explains how the National Institute of Science and Technology of Bioethanol, founded in 2008 in Brazil, became part of the main international initiatives that started to search for forms to use biomass for bioethanol production in Brazil, US and Europe. Part I reports the advances in plant cell wall composition, structure and architecture, and physical characteristics of sugarcane biomass. These discoveries are opening the way to increased efficiency of pretreatments and hydrolysis, being therefore important information for 2nd generation processes as well as for biorefinery initiatives. Part II focuses on the discovery and characterization of hydrolases from microorganisms that could be used in industrial processes. Recent advances in the search for hydrolases using metagenomics is reported. A great number of genes and enzymes from microorganisms have been discovered, affording improvement of enzyme cocktails better adapted to sugarcane biomass. Part III reports two key issues in the process of 2G ethanol, pentose fermentation and sugarcane genetics. These are the discoveries of new yeast species capable of producing ethanol more efficiently from xylose and the advances made on the sugarcane genetics, a key issue to design varieties adapted to 2G ethanol production. Part IV approaches sustainability through two chapters, one discussing the sustainability of the sugarcane agricultural and environmental system and another discussing how national and mainly international policies of Brazil regarding 2G ethanol production affected the country’s strategies to establish itself as an international player in renewable energy area.
Book Synopsis Non-conventional Yeasts: from Basic Research to Application by : Andriy Sibirny
Download or read book Non-conventional Yeasts: from Basic Research to Application written by Andriy Sibirny and published by Springer. This book was released on 2019-08-12 with total page 568 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume scopes several aspects of non-conventional yeast research prepared by the leading specialists in the field. An introduction on taxonomy and systematics enhances the reader’s knowledge on yeasts beyond established ones such as Saccharomyces cerevisiae. Biotechnological approaches that involve fungal utilization of unusual substrates, production of biofuels and useful chemicals as citric acid, glutathione or erythritol are discussed. Further, strategies for metabolic engineering based on knowledge on regulation of gene expression as well as sensing and signaling pathways are presented. The book targets researchers and advanced students working in Microbiology, Microbial Biotechnology and Biochemistry.
Book Synopsis Bioethanol by : Ayerim Y. Hernández Almanza
Download or read book Bioethanol written by Ayerim Y. Hernández Almanza and published by CRC Press. This book was released on 2022-07-07 with total page 542 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new book, Bioethanol: Biochemistry and Biotechnological Advances, presents some insightful perspectives and important advances in the bioethanol industry. The volume goes into detail on the biochemical and physiological parameters carried out by the main bioethanol-producing microorganisms as well as the discusses the potential applications that bioproducts can have and the advantages they generate. The chapter authors discuss a variety of issues, including the physiology of ethanol production by yeasts, by Zymomonas mobilis, and by Clostridium thermocellum. Other sources of biofuel, such as sweet sorghum, Agave americana L. leaves waste, and fungi are included as well. Chapters also discuss the genetic regulation and genetic engineering of principal microorganisms and then go on to address ways to increase ethanol tolerance in industrially important ethanol fermenting organisms, methods for developing sustainable fermentable substrates, and new strategies for ethanol purification. Chapters explore the design and engineering requirements for bioreactors, bioelectrosynthesis of ethanol via bioelectrochemical systems, and more. The book will be a valuable resource for faculty and students in this area as well as for scientists, researchers, and managers in the biofuel industry in the area of biofuel production, fermentation process, environmental engineering and all other related scientific areas.
Book Synopsis Fuel Ethanol Production from Sugarcane by : Thalita Peixoto Basso
Download or read book Fuel Ethanol Production from Sugarcane written by Thalita Peixoto Basso and published by BoD – Books on Demand. This book was released on 2019-01-23 with total page 232 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book offers a broad understanding of bioethanol production from sugarcane, although a few other substrates, except corn, will also be mentioned. The 10 chapters are grouped in five sections. The Fuel Ethanol Production from Sugarcane in Brazil section consists of two chapters dealing with the first-generation ethanol Brazilian industrial process. The Strategies for Sugarcane Bagasse Pretreatment section deals with emerging physicochemical methods for biomass pretreatment, and the non-conventional biomass source for lignocellulosic ethanol production addresses the potential of weed biomass as alternative feedstock. In the Recent Approaches for Increasing Fermentation Efficiency of Lignocellulosic Ethanol section, potential and research progress using thermophile bacteria and yeasts is presented, taking advantage of microorganisms involved in consolidating or simultaneous hydrolysis and fermentation processes. Finally, the Recent Advances in Ethanol Fermentation section presents the use of cold plasma and hydrostatic pressure to increase ethanol production efficiency. Also in this section the use of metabolic-engineered autotrophic cyanobacteria to produce ethanol from carbon dioxide is mentioned.
Book Synopsis Synthetic Biology of Yeasts by : Farshad Darvishi Harzevili
Download or read book Synthetic Biology of Yeasts written by Farshad Darvishi Harzevili and published by Springer Nature. This book was released on 2022-01-21 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers recent advances and future trends in yeast synthetic biology, providing readers with an overview of computational and engineering tools, and giving insight on important applications. Yeasts are one of the most attractive microbial cell factories for the production of a wide range of valuable products, including pharmaceuticals, nutraceuticals, cosmetics, agrochemicals and biofuels. Synthetic biology tools have been developed to improve the metabolic engineering of yeasts in a faster and more reliable manner. Today, these tools are used to make synthetic pathways and rewiring metabolism even more efficient, producing products at high titer, rate, and yield. Split into two parts, the book opens with an introduction to rational metabolic pathway prediction and design using computational tools and their applications for yeast systems and synthetic biology. Then, it focuses on the construction and assembly of standardized biobricks for synthetic pathway engineering in yeasts, yeast cell engineering and whole cell yeast-based biosensors. The second part covers applications of synthetic biology to produce diverse and attractive products by some well-known yeasts. Given its interdisciplinary scope, the book offers a valuable asset for students, researchers and engineers working in biotechnology, applied microbiology, metabolic engineer ing and synthetic biology.
Book Synopsis Handbook on Bioethanol by : Charles Wyman
Download or read book Handbook on Bioethanol written by Charles Wyman and published by Routledge. This book was released on 2018-05-02 with total page 289 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol is a versatile transportation fuel and fuel additive that offers excellent performance and reduced air pollution compared to conventional fuels. Its production and use adds little, if any, net release of carbon dioxide to the atmosphere, dramatically reducing the potential for global climate change. Through a sustained research program and an emerging economic competitiveness, the technology for bioethanol production is poised for immediate widespread commercial applications. Written by engineers and scientists providing a technical focus, this handbook provides the up-to-date information needed by managers, engineers, and scientists to evaluate the technology, market, and economics of this fuel, while examining the development of production required to support its commercial use.
Book Synopsis Biofuels Production by : Vikash Babu
Download or read book Biofuels Production written by Vikash Babu and published by John Wiley & Sons. This book was released on 2013-09-09 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt: The search for alternative sources of energy to offset diminishing resources of easy and cost-effective fossil fuels has become a global initiative, and fuel generated from biomass is a leading competitor in this arena. Large-scale introduction of biofuels into the energy mix could contribute to environmentally and economicaly sustainable development on a global scale. The processes and methodologies presented in this volume will offer a cutting-edge and comprehensive approach to the production of biofuels, for engineers, researchers, and students.
Book Synopsis Identifying New Yeast Strains for Bioethanol Production by : Ayesha S. Farooq
Download or read book Identifying New Yeast Strains for Bioethanol Production written by Ayesha S. Farooq and published by . This book was released on 2015 with total page 450 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advances in Yeast Biotechnology for Biofuels and Sustainability by : Achlesh Daverey
Download or read book Advances in Yeast Biotechnology for Biofuels and Sustainability written by Achlesh Daverey and published by Elsevier. This book was released on 2023-05-23 with total page 630 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advances in Yeast Biotechnology for Biofuels and Sustainability: Value-Added Products and Environmental Remediation Applications showcases the uses for engineered yeast in environmental applications, especially as an innovative source of biofuels. Beginning with a thorough review of recent advances and future potential for yeast biotechnology, the book proceeds to outline several options for biofuels, including lignocellulosic biofuels and alternative feedstock production through hydrolysis and alternative value-added products, including industrial acids and bioplastics and applications in agriculture and environmental remediation. Placing case studies at the center of each chapter, this book presents a future-focused perspective on the potential of yeast biotechnologies to support sustainability. - Lays out methods, including multiple options for generating biofuels from engineered yeast and several additional value-added products - Presents a wide variety of real-world sustainable applications for engineered yeast, with a focus on biofuels production - Provides a selection of case studies in other value-added products and applications, including bioremediation, pollution remediation, and biofertilizers in sustainable agriculture
Book Synopsis Synthetic Biology by : Christina Smolke
Download or read book Synthetic Biology written by Christina Smolke and published by John Wiley & Sons. This book was released on 2018-02-28 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: A review of the interdisciplinary field of synthetic biology, from genome design to spatial engineering. Written by an international panel of experts, Synthetic Biology draws from various areas of research in biology and engineering and explores the current applications to provide an authoritative overview of this burgeoning field. The text reviews the synthesis of DNA and genome engineering and offers a discussion of the parts and devices that control protein expression and activity. The authors include information on the devices that support spatial engineering, RNA switches and explore the early applications of synthetic biology in protein synthesis, generation of pathway libraries, and immunotherapy. Filled with the most recent research, compelling discussions, and unique perspectives, Synthetic Biology offers an important resource for understanding how this new branch of science can improve on applications for industry or biological research.
Book Synopsis Bioethanol Production from Food Crops by : Ramesh C. Ray
Download or read book Bioethanol Production from Food Crops written by Ramesh C. Ray and published by Academic Press. This book was released on 2018-08-20 with total page 472 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioethanol Production from Food Crops: Sustainable Sources, Interventions and Challenges comprehensively covers the global scenario of ethanol production from both food and non-food crops and other sources. The book guides readers through the balancing of the debate on food vs. fuel, giving important insights into resource management and the environmental and economic impact of this balance between demands. Sections cover Global Bioethanol from Food Crops and Forest Resource, Bioethanol from Bagasse and Lignocellulosic wastes, Bioethanol from algae, and Economics and Challenges, presenting a multidisciplinary approach to this complex topic. As biofuels continue to grow as a vital alternative energy source, it is imperative that the proper balance is reached between resource protection and human survival. This book provides important insights into achieving that balance. - Presents technological interventions in ethanol production, from plant biomass, to food crops - Addresses food security issues arising from bioethanol production - Identifies development bottlenecks and areas where collaborative efforts can help develop more cost-effective technology
Download or read book Yeast written by Antonio Morata and published by BoD – Books on Demand. This book was released on 2017-11-08 with total page 302 pages. Available in PDF, EPUB and Kindle. Book excerpt: Yeast - Industrial Applications is a book that covers applications and utilities of yeasts in food, chemical, energy, and environmental industries collected in 12 chapters. The use of yeasts in the production of metabolites, enzymatic applications, fermented foods, microorganism controls, bioethanol production, and bioremediation of contaminated environments is covered showing results, methodologies, and processes and describing the specific role of yeasts in them. The traditional yeast Saccharomyces cerevisiae is complemented in many applications with the use of less known non-Saccharomyces yeasts that now are being used extensively in industry. This book compiles the experience and know-how of researchers and professors from international universities and research centers.
Book Synopsis Engineering Yeast to Produce Plant Natural Products by : Yongjun Wei
Download or read book Engineering Yeast to Produce Plant Natural Products written by Yongjun Wei and published by Frontiers Media SA. This book was released on 2022-01-20 with total page 90 pages. Available in PDF, EPUB and Kindle. Book excerpt:
