Effects Of Thermal Therapy On Human Cancer Cells

Author : Olivia Nicolle Kunkel
Publisher :
ISBN 13 :
Total Pages : pages
Book Rating : 4.:/5 (116 download)

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Download or read book Effects of Acute and Repeated Heat Therapy on Prostate Cancer Cell Survival and Viability written by Olivia Nicolle Kunkel and published by . This book was released on 2019 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Background: Despite advances in treatment options, prostate cancer remains the second-most diagnosed and second leading cause of cancer-related death in men in the United States. The efficacy of conventional anti-cancer treatments is dependent, in part, on tumor blood flow and oxygenation. Tumor vasculature is abnormal relative to healthy tissue, leading to reduced blood flow and oxygen delivery, and thus, treatment resistance. An emerging adjuvant to conventional treatment to overcome resistance to radiation is heat therapy. While heat therapy may sensitize tumors to radiation via increases in tumor blood flow and thus, oxygenation, we hypothesized that heat-induced radiosensitization occurs independent, in part, of tumor blood flow or oxygenation. Methods: Clonogenic cell survival and cell viability were assessed using human prostate cancer (PC-3) cells in vitro. Individual tissue culture flasks of PC-3 cells were randomized into 6 groups, normothermic non-radiated (NT-NR, n=8), normothermic radiated (NT-R, n=8), acute hyperthermic non-radiated (HTA-NR, n=8), acute hyperthermic radiated (HTA-R, n=8), chronic (repeated) hyperthermic non-radiated (HTC-NR, n=8), and chronic (repeated) hyperthermic radiated (HTC-R, n=8) for both clonogenic cell survival and cell viability assays. For assessment of both clonogenic cell survival and cell viability, NT-NR and NT-R flasks were maintained in an incubator at 37° C for the duration of the experiment. HTA-NR and HTA-R flasks were maintained in an incubator at 37° C and heated in a separate incubator to 41° C for 60 minutes prior to radiation. HTC-NR and HTC-R flasks were maintained at 37o C and heated to 41o C for 60 minutes every 48 hours for 3 heat treatments. Non-radiated flasks were subjected to 0 Gy radiation, while radiated flasks were subjected to 2 Gy radiation. For clonogenic cell survival, cells were then plated in 60 mm tissue culture dishes at a density of 500 cells/plate and 1000 cells/plate in 5 replicates each per flask and allowed to grow for 8 days in an incubator at 37° C. Cell survival was assessed via counting the number of fixed and stained colonies >50 cells at the completion of 8 days of incubation. For cell viability, cells were plated into 96-well plates and incubated for 24, 48, and 72 hours before addition of MTT reagent for quantification of absorbance. Data are presented as mean ± SEM. Results: Clonogenic cell survival was significantly reduced between NT-NR vs. NT-R, HTA-NR, HTA-R, and HTC-R (100 % ± 9.7% vs. 59.1 % ± 5.9 %, 72.4% ± 8.5%, 40.3% ± 3.1%, and 43.3% ± 3.4%, respectively; p