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Edwardsiella Ictaluri Is Capable Of Persisting In Channel Catfish By Evading Host T Cell And Cell Death Responses
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Book Synopsis Edwardsiella Ictaluri is Capable of Persisting in Channel Catfish by Evading Host T Cell and Cell Death Responses by : Elizabeth Watts Griggs
Download or read book Edwardsiella Ictaluri is Capable of Persisting in Channel Catfish by Evading Host T Cell and Cell Death Responses written by Elizabeth Watts Griggs and published by . This book was released on 2020 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Understanding Molecular Mechanisms of Host-Edwardsiella Ictaluri Interaction by : Nawar Al-Janabi
Download or read book Understanding Molecular Mechanisms of Host-Edwardsiella Ictaluri Interaction written by Nawar Al-Janabi and published by . This book was released on 2017 with total page 118 pages. Available in PDF, EPUB and Kindle. Book excerpt: Catfish, the "king" of the U.S. aquaculture, is threatened by a severe, systemic bacterial disease known as enteric septicemia of catfish (ESC). This disease causes high mortality and massive economic losses in cultured channel catfish (Ictalurus punctatus) in the United States. E. ictaluri penetrates catfish intestinal epithelia quickly and establishes a systemic infection rapidly. However, our knowledge on catfish intestine and E. ictaluri interaction is very limited. In Particular, catfish intestinal immune responses and virulence genes needed by E. ictaluri to evade host defenses are not well understood. Hence, our long-term goal is to identify the molecular mechanisms of E. ictaluri-host interactions. The overall objectives of this study were to understand catfish immune responses to E. ictaluri infection and determine essential genes of E. ictaluri during the intestinal invasion. To accomplish the overall objectives of this research, intestinal ligated loops were constructed surgically in live catfish and loops were injected with wild-type E. ictaluri and two live attenuated E. ictaluri vaccine strains developed recently by our research group. We first determined catfish intestinal immune responses against E. ictaluri wild-type and live attenuated vaccine strains. Then, we analyzed the global gene iii expression patterns of wild-type E. ictaluri and vaccine strains during catfish intestinal invasion using high throughput RNA-Seq technology. Results showed a moderate level of neutrophil and B cell infiltration correlated with significantly lower expression of TNF-[alpha], CD4-1, and CD8-[alpha] in the vaccine injected intestinal tissue compared to that of wild-type injected intestinal tissue. Further, RNA-Seq data analysis showed the prominent expression of genes related to bacterial secretion systems, ATP production processes, and multidrug resistance (MDR) efflux pumps in wild-type E. ictaluri. In contrast, the prominently expressed genes in vaccine strains were related to the phosphotransferase system and sugar metabolism processes. All these data suggest that our live attenuated vaccines are capable of triggering effective immune responses in catfish without causing damage to the host.
Book Synopsis Antigen Presenting Cells-Mediated Innate and Adaptive Immune Responses to Live Attenuated Edwardsiella Ictaluri Vaccines in Channel Catfish by :
Download or read book Antigen Presenting Cells-Mediated Innate and Adaptive Immune Responses to Live Attenuated Edwardsiella Ictaluri Vaccines in Channel Catfish written by and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Vaccination against intracellular pathogens requires generation of pool of memory T cells, which can respond upon infection and mediate immune responses by either killing of infected host cells or induce killing mechanisms in infected cells. T cell-inducing vaccines aim to deliver the antigen to antigen presenting cells (APCs) by presenting on MHC molecules thus bridging innate and adaptive immunity. The intracellular pathogen Edwardsiella ictaluri causes enteric septicemia of catfish (ESC), which is a devastating disease in catfish industry. E. ictaluri can survive in professional phagocytes and use them as an infection source. Two new live attenuated vaccine (LAV) strains, EiDELTAevpB and ESC-NDKL, were developed by our group. However, the role of LAVs in phagocytosis, bacterial killing, and antigen presentation is unexplored. Therefore, further research is necessary to determine immune responses in channel catfish against LAVs. The long-term goal of this project is to identify immunological APC-dependent mechanisms that underscore E. ictaluri pathogenesis to enable development of effective control strategies for ESC. The overall goal of this project is to assess the role of three professional APCs, dendritic cells (DCs), macrophages and B cells in the LAV-induced innate and adaptive immune responses in catfish. The central hypothesis is that efficacious LAV strains will enhance phagocytosis and microbial killing, and promote the generation of T cells that regulate and control protective B cell-mediated immunity. The rationale for this research is that more detailed knowledge about phenotype and function of catfish APCs will not only help gain insight into the evolution of vertebrate adaptive immune system but will provide valuable information for development and optimization of immunotherapies and vaccination protocols for aquaculture use. In this study, we first identified DC-like cells in immune-related organs of catfish an
Book Synopsis Aspects of the Host Response of Channel Catfish, Ictalurus Punctatus, to Edwardsiella Ictaluri by : Paul R. Waterstrat
Download or read book Aspects of the Host Response of Channel Catfish, Ictalurus Punctatus, to Edwardsiella Ictaluri written by Paul R. Waterstrat and published by . This book was released on 1988 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Humoral and Cell-mediated Immune Response of Channel Catfish, Ictalurus Punctatus, to Edwardsiella Ictaluri by : Somsak Vinitnantharat
Download or read book Humoral and Cell-mediated Immune Response of Channel Catfish, Ictalurus Punctatus, to Edwardsiella Ictaluri written by Somsak Vinitnantharat and published by . This book was released on 1991 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Immune Response of Channel Catfish to the Edwardsiella Ictaluri Endotoxin by : Jean-Yves Mevel
Download or read book Immune Response of Channel Catfish to the Edwardsiella Ictaluri Endotoxin written by Jean-Yves Mevel and published by . This book was released on 1985 with total page 112 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Channel Catfish, Ictalurus Punctatus, T-lymphocyte Mitogenic Responses to Outer Membrane Proteins and LPS of the Gram Negative Rod Bacterium, Edwardsiella Ictaluri by : Barry Revis Hamilton
Download or read book Channel Catfish, Ictalurus Punctatus, T-lymphocyte Mitogenic Responses to Outer Membrane Proteins and LPS of the Gram Negative Rod Bacterium, Edwardsiella Ictaluri written by Barry Revis Hamilton and published by . This book was released on 1995 with total page 106 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Responses of Channel Catfish, Ictalurus Punctatus, T-lymphocytes to Outer Membrane Proteins of Edwardsiella Ictaluri by : Marlin Ann Lawson
Download or read book Responses of Channel Catfish, Ictalurus Punctatus, T-lymphocytes to Outer Membrane Proteins of Edwardsiella Ictaluri written by Marlin Ann Lawson and published by . This book was released on 1998 with total page 110 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Antigen Presenting Cells-mediated Innate and Adaptive Immune Responses to Live Attenuated Edwardsiella Ictaluri Vaccines in Channel Catfish by : Adef Kordon
Download or read book Antigen Presenting Cells-mediated Innate and Adaptive Immune Responses to Live Attenuated Edwardsiella Ictaluri Vaccines in Channel Catfish written by Adef Kordon and published by . This book was released on 2018 with total page 178 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Innate and Adaptive Immune Responses of Channel Catfish to Edwardsiella Ictaluri Wild Type and Live Attenuated Vaccine Candidates by : Ozgur Erdogan
Download or read book Innate and Adaptive Immune Responses of Channel Catfish to Edwardsiella Ictaluri Wild Type and Live Attenuated Vaccine Candidates written by Ozgur Erdogan and published by . This book was released on 2020 with total page 57 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edwardsiella ictaluri causes enteric septicemia of catfish (ESC), a devastating disease in the channel catfish industry. Our research group has developed several E. ictaluri live attenuated vaccine (LAV) candidates (Ei(delta)evpB, Ei(delta)evpB(delta)fur, Ei(delta)evpB(delta)hfq, Ei(delta)evpB(delta)fur(delta)hfq), which were able to stimulate an immune response in vaccinated channel catfish and reduce ESC. However, innate, and adaptive immune responses in the lymphoid tissues of channel catfish to these LAVs are not known well. The overall goal of the project is to determine the role of adaptive and innate immune responses in catfish after vaccination with LAVs. Analysis of innate and adaptive immune-related gene expressions showed that the LAVs induced expression of adaptive immune-related genes in lymphoid tissues with less inflammation compared to wild type control. Also, the LAVs induced the expression of IgM in the sera of catfish.
Book Synopsis Edwardsiella Ictaluri by : Thomas J. Baldwin
Download or read book Edwardsiella Ictaluri written by Thomas J. Baldwin and published by . This book was released on 1992 with total page 314 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis The Efficacy of Romet-30 Against Edwardsiella Ictaluri in the Channel Catfish, Ictalurus Punctatus by : Alton Timothy Leard
Download or read book The Efficacy of Romet-30 Against Edwardsiella Ictaluri in the Channel Catfish, Ictalurus Punctatus written by Alton Timothy Leard and published by . This book was released on 1998* with total page 176 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Longevity of Edwardsiella Ictaluri in the Organs of Experimentally Infected Channel Catfish, Ictalurus Punctatus by : Thobekile Nolan Mqolomba
Download or read book Longevity of Edwardsiella Ictaluri in the Organs of Experimentally Infected Channel Catfish, Ictalurus Punctatus written by Thobekile Nolan Mqolomba and published by . This book was released on 1989 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Characterization of Type VI Secretion System in Edwardsiella Ictaluri by : Safak Kalindamar
Download or read book Characterization of Type VI Secretion System in Edwardsiella Ictaluri written by Safak Kalindamar and published by . This book was released on 2017 with total page 74 pages. Available in PDF, EPUB and Kindle. Book excerpt: Edwardsiella ictaluri causes enteric septicemia of catfish (ESC), which is one of the most important bacterial diseases causing significant economic losses in the US catfish industry. Understanding the virulence mechanisms of E. ictaluri plays a vital role to develop preventives, such as vaccines for the disease. Therefore, further research is necessary to discover the new virulence mechanisms of this pathogen. The long-term goal of our group is to determine the mechanism of E. ictaluri pathogenesis and to develop effective live attenuated vaccines against ESC. The overall goal of this project is to understand the role of Type 6 secretion system (T6SS) in E. ictaluri virulence and determine the safety and efficacy of T6SS mutants in the catfish host. The central hypothesis is that T6SS in E. ictaluri provide an ability to invade the host cells and survive inside of the channel catfish neutrophils and macrophages, and mutation of T6SS genes will cause attenuation of the bacterial virulence. The rationale for the proposed research is that characterization of the T6SS in E. ictaluri will enlighten its role in E. ictaluri virulence, and T6SS genes can be targeted to develop live attenuated vaccines. In this study, we first constructed mutants of individual T6SS genes and a double mutant. The persistence, virulence, and vaccine efficacy of T6SS mutants were determined in the catfish fingerlings and fry infection model. The T6SS mutants Ei[delta]evpC, Ei[delta]evpC[delta]hcp2, Ei[delta]evpD, Ei[delta]evpE, Ei[delta]evpG, Ei[delta]evpJ, and Ei[delta]evpK were significantly attenuated and provided better protection against E. ictaluri 93-146 in channel catfish fingerlings. The role of T6SS mutants in adhesion and invasion of in vitro catfish epithelial indicated that Ei[delta]evpN, Ei[delta]evpO, and Ei[delta]evpP significantly were less adherent and invasive. The survival and replication of T6SS mutants in in vitro catfish peritoneal macrophages cell line showed that T6SS mutants could survive up to 6 hours after phagocyted by catfish macrophages. The survival and resistance of T6SS mutants to stress conditions present in macrophages phagosome showed that hydrogen peroxide could limit the growth of T6SS mutants in BHI and minimal medium. Ei[delta]evpA, Ei[delta]evpH, Ei[delta]evpM, Ei[delta]evpN, and Ei[delta]evpO exhibited a significant growth decrease.
Book Synopsis Evaluation of Pathogenesis, Vaccination, and Stress in Channel Catfish Experimentally Infected with Edwardsiella Ictaluri by : David Wise
Download or read book Evaluation of Pathogenesis, Vaccination, and Stress in Channel Catfish Experimentally Infected with Edwardsiella Ictaluri written by David Wise and published by . This book was released on 1994 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Identification of Virulence Factors in Edwardsiella Ictaluri by :
Download or read book Identification of Virulence Factors in Edwardsiella Ictaluri written by and published by . This book was released on 2013 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: Edwardsiella ictaluri is the causative agent of enteric septicemia of catfish (ESC), which is one of the most important diseases impacting the US catfish industry. Though this disease has been very common, progress has been slow to find an economical and practical treatment method. Our long-term goal is to determine the mechanisms of E. ictaluri virulence in ESC. The overall objective of this study was to identify E. ictaluri genes required for host encounter and serum resistance and to determine their roles in pathogenesis. The central hypothesis is that E. ictaluri must differentially regulate its genes to invade fish and evade host defenses, thus, mutation of these differentially expressed genes (DEG) should cause attenuation of E. ictaluri virulence. To test this hypothesis, we first determined the global gene expression patterns of the wild type (wt) E. ictaluri 93-146 and EiAKMut02 mutant during catfish encounter and serum exposure using microarray analysis. Results indicated that in E. ictaluri wt, 377 and 16 DEGs were identified during host encounter and serum exposure, respectively. In EiAKMut02, 82 and 296 DEGs were identified during host encounter and serum experiment. Through functional analysis using Blast2GO, PSORTb, Host Pathogen Interaction Database (HPIDB), and Microbe Virulence Database (MVirDB), 38 DEGs in 9 KEGG pathways have been identified as potential virulence factors. The KEGG pathways represented were 1) bacterial secretion system including T3SS and T6SS, 2) ABC transporters including cystine transport system, iron complex transport system, d-methionine transport system, arginine transport system, thiamine transport system, and molybdate transport system, 3) protein export, 4) flagellar assembly, 5) two-component system, 6) bacterial chemotaxis, 7) ascorbate and aldarate metabolism, 8) phosphotransferase system, and 9) metabolic pathways. In order to understand their role in the E. ictaluri virulence, selected DEGs were in-frame deleted by allelic exchange, and their virulence and efficacy were characterized in channel catfish fingerlings. Our results showed that the virulence of E. ictaluri ssaV and yscR mutants was completely attenuated while their efficacies were moderate in catfish fingerlings. These results support that the T3SS and T6SS, ABC transporters, protein export, and flagella seem to be important in E. ictaluri virulence.
Book Synopsis Response and Correlated Response to Selection of Channel Catfish, Ictalurus Punctatus, for Resistance to Edwardsiella Ictaluri, Flexibacter Columnaris, Low Levels of Dissolved Oxygen, and Toxic Level of Nitrite by : Phillip Lynch Waters
Download or read book Response and Correlated Response to Selection of Channel Catfish, Ictalurus Punctatus, for Resistance to Edwardsiella Ictaluri, Flexibacter Columnaris, Low Levels of Dissolved Oxygen, and Toxic Level of Nitrite written by Phillip Lynch Waters and published by . This book was released on 2001 with total page 104 pages. Available in PDF, EPUB and Kindle. Book excerpt:
