Read Books Online and Download eBooks, EPub, PDF, Mobi, Kindle, Text Full Free.
Development Of Multidimensional Liquid Phase Protein Separations For Proteomic Analysis
Download Development Of Multidimensional Liquid Phase Protein Separations For Proteomic Analysis full books in PDF, epub, and Kindle. Read online Development Of Multidimensional Liquid Phase Protein Separations For Proteomic Analysis ebook anywhere anytime directly on your device. Fast Download speed and no annoying ads. We cannot guarantee that every ebooks is available!
Book Synopsis Development of Multidimensional Liquid Phase Protein Separations for Proteomic Analysis by : Bobby Fachrizal Assiddiq
Download or read book Development of Multidimensional Liquid Phase Protein Separations for Proteomic Analysis written by Bobby Fachrizal Assiddiq and published by . This book was released on 2009 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Multidimensional Liquid Chromatographic Separations for Proteomics by : Karl Burgess
Download or read book Multidimensional Liquid Chromatographic Separations for Proteomics written by Karl Burgess and published by . This book was released on 2008 with total page 246 pages. Available in PDF, EPUB and Kindle. Book excerpt: Sample complexity is one of the key challenges facing contemporary proteomic analysis. A variety of methods is commonly employed to reduce this complexity, both at an intact protein and digested peptide level. For complex lysates containing many thousands of proteins, orthogonal (mutually independent), multidimensional separation methods must be employed to provide sufficient resolution to characterize the appropriate number of different species. The most common of these methods are two dimensional gel electrophoresis (2DGE) of proteins and multidimensional liquid chromatographic separation (MuDPIT) of peptides, which rely on isoelectric focusing followed by mass separation in the former, and ion exchange followed by reversed phase separation in the latter. These methods have significant drawbacks in terms of sample bias, sample preparation and reproducibility, and therefore a new methodology that combines the positive aspects of both separation technologies in an automatable, reproducible form is highly desirable. New developments in column technology have allowed rapid improved-resolution separation of intact proteins in complex samples, coupled to improved methodology for peptide and protein identification. The separation of complex protein mixtures using both on- and off-line 2D liquid chromatography using derivitized polystyrene-divinylbenzene (PS-DVB) pellicular ion-exchange resins and PS-DVB monolithic reversed-phase columns is described. Proteolytic digestion of the fractions followed by rapid liquid chromatography-tandem mass spectrometry was used to complete the analysis. An alternative methodology, relying on direct analysis of the second dimension eluents by top-down (mass spectrometric analysis of intact proteins) methodology, using an Apex IV 12 T Fourier-transform ion cyclotron resonance mass spectrometer (Bruker Daltonics) and an HCT ion trap (Bruker Daltonics) equipped with electron transfer dissociation has allowed in-depth analysis of intact proteins. Sample types investigated to establish the utility of the methodology include bacterial lysates (Bordetella parapertusis, and Escherichia coli), a eukaryotic parasite (Leishmania donovani), and transformed human cell lines.
Book Synopsis Development of Novel Multi-dimensional Separation Methods for Analysis of the Proteome Using Iso-electric Focusing, Reversed Phase HPLC, MALDI TOF/MS and ESI TOF/MS. by : Daniel B. Wall
Download or read book Development of Novel Multi-dimensional Separation Methods for Analysis of the Proteome Using Iso-electric Focusing, Reversed Phase HPLC, MALDI TOF/MS and ESI TOF/MS. written by Daniel B. Wall and published by . This book was released on 2001 with total page 334 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Modern Proteomics – Sample Preparation, Analysis and Practical Applications by : Hamid Mirzaei
Download or read book Modern Proteomics – Sample Preparation, Analysis and Practical Applications written by Hamid Mirzaei and published by Springer. This book was released on 2016-12-14 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume serves as a proteomics reference manual, describing experimental design and execution. The book also shows a large number of examples as to what can be achieved using proteomics techniques. As a relatively young area of scientific research, the breadth and depth of the current state of the art in proteomics might not be obvious to all potential users. There are various books and review articles that cover certain aspects of proteomics but they often lack technical details. Subject specific literature also lacks the broad overviews that are needed to design an experiment in which all steps are compatible and coherent. The objective of this book was to create a proteomics manual to provide scientists who are not experts in the field with an overview of: 1. The types of samples can be analyzed by mass spectrometry for proteomics analysis. 2. Ways to convert biological or ecological samples to analytes ready for mass spectral analysis. 3. Ways to reduce the complexity of the proteome to achieve better coverage of the constituent proteins. 4. How various mass spectrometers work and different ways they can be used for proteomics analysis 5. The various platforms that are available for proteomics data analysis 6. The various applications of proteomics technologies in biological and medical sciences This book should appeal to anyone with an interest in proteomics technologies, proteomics related bioinformatics and proteomics data generation and interpretation. With the broad setup and chapters written by experts in the field, there is information that is valuable for students as well as for researchers who are looking for a hands on introduction into the strengths, weaknesses and opportunities of proteomics.
Book Synopsis Multidimensional Liquid Chromatography by : Steven A. Cohen
Download or read book Multidimensional Liquid Chromatography written by Steven A. Cohen and published by John Wiley & Sons. This book was released on 2008-05-09 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.
Book Synopsis Development of Fully Automatable Multidimensional Liquid Chromatography (MDLC) with Online Tandem Mass Spectrometry for Shotgun Proteomics by : Pak-Wing Kong
Download or read book Development of Fully Automatable Multidimensional Liquid Chromatography (MDLC) with Online Tandem Mass Spectrometry for Shotgun Proteomics written by Pak-Wing Kong and published by Open Dissertation Press. This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Development of Fully Automatable Multidimensional Liquid Chromatography (MDLC) With Online Tandem Mass Spectrometry for Shotgun Proteomics" by Pak-wing, Kong, 江柏榮, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: Proteomics is the systematic study of the proteome: the total protein expression of a cell or tissue under specified conditions. The multiplicity and complexity of proteins in cells requires sensitive, selective, and comprehensive methodologies for their distinction and characterization. Multidimensional liquid chromatography (MDLC) coupled with biological tandem mass spectrometry (MS/MS) is uniquely suited to fulfill those requirements and has become an indispensable tool in MS-based proteomics. Our laboratory has developed an online high-/low-pH reversed-phase/reversed-phase (RP-RP) LC system exhibiting fully automatable and reproducible performance. It is a promising alternative to the strong cation exchange/reversed-phase (SCX-RP) system commonly used in high-throughput comprehensive proteomics analyses. The first part of this Thesis (Chapter 2) describes the development of a variant of the high-/low-pH RP-RP platform-RP-SCX-RP-that integrates an additional SCX trap column between the two RP columns to enhance sample recovery. This new system allows the detection of larger numbers of hydrophilic peptides. Indeed, in the analyses of a lysate of Arabidopsis chloroplast proteins, it identified approximately 25% more non-redundant proteins than those identified using the previous version of the RP-RP system. The modified platform has been extended for the online removal of sodium dodecyl sulfate and other excess interference chemicals used in Isobaric Tags for Relative and Absolute Quantification (iTRAQ) reactions, thereby avoiding the need for time-consuming offline SCX clean-up prior to RP-RP separation in the quantitative proteomics analyses of crude biological samples at low-microgram levels. A novel online three-dimensional liquid chromatography (3DLC) system was derived from the RP-SCX-RP design, exhibiting remarkably enhanced orthogonality, resolution, and peak capacity. Peptides were separated in the first-dimension high-pH RP column based on their hydrophobicity, followed by sub-fractionation in the second-dimension SCX column, primarily based on charge; the third dimension was a typical low-pH RP separation, prior to MS analysis. The overall performance of the system was evaluated through analysis of a cell lysate of mouse embryonic fibroblasts. Relative to the two-dimensional high-/low-pH RP-RP system, the new 3D system yielded significant increases in the number of unique peptides and proteins identified, making it a good alternative to SCX-RP and high-/low-pH RP-RP as an efficient automated MDLC platform for high-throughput shotgun proteomics. An optimized and miniaturized variant of the three-dimensional LC platform was also developed. Its simplified setup and operation, by decreasing the number of six-port switching valves (from three to two) and the number of SCX fractionation steps, minimized both the potential sample loss and the total analysis time (by ca. 30%). Thus, a variety of novel, automatable, and robust RP-SCX-RP-based MDLC platforms have been developed for high-throughput qualitative and quantitative analysis. The performance of these systems complements conventional MDLC systems, with enhanced quality, quantity, reproducibility, and throughput of protein identification and quantification. DOI: 10.5353/th_b4819927 Subjects: Liquid chromatography Mass spectrometry
Book Synopsis New Front-end Separation Approaches for Top-down Proteomics by : Eli Larson
Download or read book New Front-end Separation Approaches for Top-down Proteomics written by Eli Larson and published by . This book was released on 2023 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Top-down mass spectrometry (MS) and top-down proteomics have become indispensable tools to characterize and identify unique proteoforms. Proteoforms are defined as all protein products of a single gene, including splicing variants, mutants, and post-translationally modified forms. Although the development of new MS capabilities has exploded in recent years, the comparative underdevelopment of intact protein separations and data processing solutions has prevented full realization of the benefits of top-down. To address these challenges, I have developed new front-end separation approaches for top-down proteomics, beginning with targeted separations for multi-attribute analysis of antibody-drug conjugates (ADCs) and later developing an online two-dimensional liquid chromatography (2DLC) method to expand global proteome coverage by top-down proteomics. Chapter 1 focuses on recent advances in front-end separations and data processing solutions for top-down proteomics and introduces top-down applications to antibody-based therapeutic analysis. Chapter 2 and chapter 3 detail new targeted separation approaches for monoclonal antibodies and ADCs. Chapter 2 reports reversed phase liquid chromatography (RPLC) coupled to high-resolution Fourier transform ion cyclotron resonance MS for top-down analysis of a reduced cysteine-linked ADC. Chapter 3 details the development of a native complex-down workflow using trapped ion mobility spectrometry-MS with a cysteine-linked ADC and parent mAb under non-denaturing conditions (Chapter 3). Chapter 4 reports a new software package designed to address the challenges associated with native top-down proteomics, MASH Native. Chapter 5 focuses on the development of a new online 2DLC method coupling serial size exclusion and RPLC to expand global top-down proteome coverage, with application to human heart extract. Appendix I reports a shotgun proteomic approach to characterize the impact of splicing factor RNA binding motif 20 knockout on the rat heart proteome and identifies targets for follow-up analysis by top-down proteomics. The developed techniques detailed here will address key challenges to front-end separation in the field of top-down proteomics, expanding analytical capabilities for future targeted and discovery studies.
Book Synopsis Fully Automatable Multidimensional Liquid Chromatography with Online Tandem Mass Spectrometry for Proteomics and Glycoproteomics by : Yun Zhao
Download or read book Fully Automatable Multidimensional Liquid Chromatography with Online Tandem Mass Spectrometry for Proteomics and Glycoproteomics written by Yun Zhao and published by . This book was released on 2017-01-26 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: This dissertation, "Fully Automatable Multidimensional Liquid Chromatography With Online Tandem Mass Spectrometry for Proteomics and Glycoproteomics" by Yun, Zhao, 赵赟, was obtained from The University of Hong Kong (Pokfulam, Hong Kong) and is being sold pursuant to Creative Commons: Attribution 3.0 Hong Kong License. The content of this dissertation has not been altered in any way. We have altered the formatting in order to facilitate the ease of printing and reading of the dissertation. All rights not granted by the above license are retained by the author. Abstract: This dissertation reports the development of novel, fully automatable, online multidimensional liquid chromatography (MDLC) technologies and methodologies to accelerate proteomics and glycomics mapping from complex biological samples. Chapter 2 reports the development of an online two-dimensional (2D) liquid chromatography (LC) system-with separations based on hydrophilic interactions in the first dimension and low-pH reversed-phase (RP) separation (peptide hydrophobicity) in the second-that operated with high resolution and orthogonality. This hydrophilic interaction liquid chromatography (HILIC) -RP platform featured an RP trap column plus a mixing loop before the first dimension to facilitate direct aqueous sample loading; an additional sample loop plus a strong cation exchange (SCX) trap column was implemented to circumvent the problem of solvent incompatibility between the two columns. The performance of this system was benchmarked through analysis of the proteome of Saccharomyces cerevisiae, resulting in the identification of more than 2000 proteins with abundances spanning from 40 to 〖10〗 DEGREES6 copies/cell. Chapter 3 reports a novel online three-dimensional (3D) HILIC-SCX-RP coupled with porous graphitic carbon (PGC) LC platform derived from the HILIC-RP design, featuring additional SCX fractionations, operating through a charge-centric separation mechanism, to extend the separation efficiency and platform orthogonality; the PGC column was integrated to recapture non-retained hydrophilic analytes for concomitant analyses of both hydrophilic and hydrophobic analytes within the same sample injection event. This integrated technology exhibited superior performance for the proteomics analyses of the total lysate of primary cerebellar granule neurons (CGNs) and cynomolgus monkey brain tissue, with enhanced protein and proteome coverage. One of the most comprehensive CGNs proteome to date was characterized: in total, 2201 proteins and 16,937 unique peptides. This 3D HILIC-SCX-RP/PGC system allowed the first detailed and simultaneous N-glycomics and N-glycoproteomics analyses of cynomolgus monkey plasma, establishing a glycan library containing 122 proposed N-glycans with confirmed complementary sites of N-glycosylation; 38 N-glycolylneuraminic acid (NeuGc)-containing N-glycans were also verified through tandem mass spectrometry for the first time. Finally, Chapter 4 describes the first online 2D PGC-RP LC system with dual sample traps that allowed the implementation of shotgun proteomics and glycomics analyses using less-sophisticated instrumentation. The PGC-platform operated through a mixed mode of mechanisms for peptide separation, taking advantage of both planar contact area-based interactions and hydrophobicity, allowing elimination of the aforementioned RP trap column, mixing loop, and related switching valves for sample loading; thus, this system could be readily assembled on a commercially available MDLC system with minimal modifications. The dual-trap column configuration was adopted, offering desirable high-throughput with almost no idle time for sample fractionation, trapping, or desalting. This 2D PGC-RP technology performed well, as judged by the results of proteomics and glycoproteomics analyses of cerebellar granule neurons lysates and cynomolgus monkey plasma. A comparison of the HILIC-SCX-RP and PGC-RP analyses in
Book Synopsis Separation Methods In Proteomics by : Gary B. Smejkal
Download or read book Separation Methods In Proteomics written by Gary B. Smejkal and published by CRC Press. This book was released on 2005-12-12 with total page 532 pages. Available in PDF, EPUB and Kindle. Book excerpt: Driven by the widespread growth of proteomic practices, protein separation techniques have been refined to minimize variability, optimize particular applications, and adapt to user preferences in the analysis of proteins. Separation Methods in Proteomics provides a comprehensive examination of all major separation techniques for proteomic
Book Synopsis Towards the Development of a Proteomics Workflow for High-throughput Protein Biomarker Discovery by : Mark J. Wall
Download or read book Towards the Development of a Proteomics Workflow for High-throughput Protein Biomarker Discovery written by Mark J. Wall and published by . This book was released on 2010 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Two-Dimensional Liquid Chromatography by : Oliver Jones
Download or read book Two-Dimensional Liquid Chromatography written by Oliver Jones and published by Springer Nature. This book was released on 2020-07-13 with total page 86 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book addresses the growing interest in the field of two-dimensional liquid chromatography (2DLC), a powerful approach to increasing resolution, available peak capacity, and selectivity in analytical chromatography. 2DLC is suitable for many applications, including in the pharmaceutical and polymer industries and the omic sciences (metabolomics, lipidomics and proteomics). Thanks to recent advances in technology and software the instrumentation needed to perform 2D-LC is broadly available to the analytical community in both industry and academia. Indeed, the technique can now be considered ready for application in R&D as well as in QA and QC labs, yet it is not widely known about outside academic laboratories and is rarely taught at the undergraduate level. This book outlines the main principles and features of 2D-LC (including comprehensive and heart-cutting modes, method development and real world applications) to enable modern analysts to start using this fascinating technique. The book offers an ideal starting point for those wishing to get into 2D-LC and will also be of interest to more experienced scientists in the field.
Book Synopsis Development of Multi-dimensional Separation Schemes for the Analysis of Membrane Proteins by : Kimberly A. Schneider
Download or read book Development of Multi-dimensional Separation Schemes for the Analysis of Membrane Proteins written by Kimberly A. Schneider and published by . This book was released on 2003 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis HPLC of Peptides and Proteins by : Marie-Isabel Aguilar
Download or read book HPLC of Peptides and Proteins written by Marie-Isabel Aguilar and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 411 pages. Available in PDF, EPUB and Kindle. Book excerpt: The introduction of high-performance liquid chromatography (HPLC) to the analysis of peptides and proteins some 25 years ago revolutionized the biological sciences by enabling the rapid and sensitive analysis of peptide and protein structure through the exquisite speed, sensitivity, and resolution that can be easily obtained. Today, HPLC in its various modes has become the pivotal technique in the characterization of peptides and proteins and currently plays a critical role in both our understanding of biological processes and in the development of peptide- and protein-based pharmaceuticals. The number of applications of HPLC in peptide and protein purification continues to expand at an extremely rapid rate. Solid-phase peptide synthesis and recombinant DNA techniques have allowed the production of large quantities of peptides and proteins that need to be highly purified. HPLC techniques are also used extensively in the isolation and characterization of novel proteins that will become increasingly important in the postgenomic age. The design of multidimensional purification schemes to achieve high levels of product purity further demonstrates the power of HPLC techniques not only in the characterization of cellular events, but also in the production of pepti- and protein-based therapeutics. HPLC continues to be at the heart of the analytical techniques with which scientists in both academia and in industry must arm themselves to be able to fully characterize the identity, purity, and potency of peptides and proteins.
Book Synopsis Mass Spectrometry-Based Chemical Proteomics by : W. Andy Tao
Download or read book Mass Spectrometry-Based Chemical Proteomics written by W. Andy Tao and published by John Wiley & Sons. This book was released on 2019-07-10 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: PROVIDES STRATEGIES AND CONCEPTS FOR UNDERSTANDING CHEMICAL PROTEOMICS, AND ANALYZING PROTEIN FUNCTIONS, MODIFICATIONS, AND INTERACTIONS—EMPHASIZING MASS SPECTROMETRY THROUGHOUT Covering mass spectrometry for chemical proteomics, this book helps readers understand analytical strategies behind protein functions, their modifications and interactions, and applications in drug discovery. It provides a basic overview and presents concepts in chemical proteomics through three angles: Strategies, Technical Advances, and Applications. Chapters cover those many technical advances and applications in drug discovery, from target identification to validation and potential treatments. The first section of Mass Spectrometry-Based Chemical Proteomics starts by reviewing basic methods and recent advances in mass spectrometry for proteomics, including shotgun proteomics, quantitative proteomics, and data analyses. The next section covers a variety of techniques and strategies coupling chemical probes to MS-based proteomics to provide functional insights into the proteome. In the last section, it focuses on using chemical strategies to study protein post-translational modifications and high-order structures. Summarizes chemical proteomics, up-to-date concepts, analysis, and target validation Covers fundamentals and strategies, including the profiling of enzyme activities and protein-drug interactions Explains technical advances in the field and describes on shotgun proteomics, quantitative proteomics, and corresponding methods of software and database usage for proteomics Includes a wide variety of applications in drug discovery, from kinase inhibitors and intracellular drug targets to the chemoproteomics analysis of natural products Addresses an important tool in small molecule drug discovery, appealing to both academia and the pharmaceutical industry Mass Spectrometry-Based Chemical Proteomics is an excellent source of information for readers in both academia and industry in a variety of fields, including pharmaceutical sciences, drug discovery, molecular biology, bioinformatics, and analytical sciences.
Book Synopsis Advanced Mass Spectrometry for Food Safety and Quality by :
Download or read book Advanced Mass Spectrometry for Food Safety and Quality written by and published by Elsevier. This book was released on 2015-05-08 with total page 733 pages. Available in PDF, EPUB and Kindle. Book excerpt: Advanced Mass Spectrometry for Food Safety and Quality provides information on recent advancements made in mass spectrometry-based techniques and their applications in food safety and quality, also covering the major challenges associated with implementing these technologies for more effective identification of unknown compounds, food profiling, or candidate biomarker discovery. Recent advances in mass spectrometry technologies have uncovered tremendous opportunities for a range of food-related applications. However, the distinctive characteristics of food, such as the wide range of the different components and their extreme complexity present enormous challenges. This text brings together the most recent data on the topic, providing an important resource towards greater food safety and quality. Presents critical applications for a sustainable, affordable and safe food supply Covers emerging problems in food safety and quality with many specific examples. Encompasses the characteristics, advantages, and limitations of mass spectrometry, and the current strategies in method development and validation Provides the most recent data on the important topic of food safety and quality
Book Synopsis The Expanding Role of Mass Spectrometry in Biotechnology by : Gary Siuzdak
Download or read book The Expanding Role of Mass Spectrometry in Biotechnology written by Gary Siuzdak and published by . This book was released on 2006 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Covers the basic concepts in mass spectrometry as well as advanced topics including protein identification/protein structural analysis, carbohydrate and oligonucleotide analysis. Topics also include pharmacokinetics, high throughput screening, and the recent development of mass spectrometry in clinical diagnosis.
Book Synopsis Principles and Applications of Clinical Mass Spectrometry by : Nader Rifai
Download or read book Principles and Applications of Clinical Mass Spectrometry written by Nader Rifai and published by Elsevier. This book was released on 2018-06-26 with total page 218 pages. Available in PDF, EPUB and Kindle. Book excerpt: Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is a concise resource for quick implementation of mass spectrometry methods in clinical laboratory work. Focusing on the practical use of these techniques, the first half of the book covers principles of chromatographic separations, principles and types of mass spectrometers, and sample preparation for analysis; the second half outlines the main applications of this technology within clinical laboratory settings, including determination of small molecules and peptides, as well as pathogen identification. A thorough yet succinct guide to using mass spectrometry technology in the clinical laboratory, Principles and Applications of Clinical Mass Spectrometry: Small Molecules, Peptides, and Pathogens is an essential resource for chemists, pharmaceutical and biotech researchers, certain government agencies, and standardization groups. Provides concrete examples of the main applications of mass spectrometry technology Describes current capabilities of the LC- and MS-based analytical methods Details methods for successful analytical work in the field
