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Development And Application Of Two Dimensional Separation Systems Coupled To Mass Spectrometry Incorporating Capillary Electrophoresis
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Book Synopsis Development and Application of Two-dimensional Separation Systems Coupled to Mass Spectrometry Incorporating Capillary Electrophoresis by : Kevin Jooß
Download or read book Development and Application of Two-dimensional Separation Systems Coupled to Mass Spectrometry Incorporating Capillary Electrophoresis written by Kevin Jooß and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Development and Application of Two-dimensional Capillary Electrophoresis for Online Mass Spectrometry of CE(SDS)-separated Proteins by : Jennifer Römer
Download or read book Development and Application of Two-dimensional Capillary Electrophoresis for Online Mass Spectrometry of CE(SDS)-separated Proteins written by Jennifer Römer and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Multidimensional Liquid Chromatography by : Steven A. Cohen
Download or read book Multidimensional Liquid Chromatography written by Steven A. Cohen and published by John Wiley & Sons. This book was released on 2008-05-09 with total page 490 pages. Available in PDF, EPUB and Kindle. Book excerpt: Multidimensional Liquid Chromatography (MDLC) is a very powerful separation technique for analyzing exceptionally complex samples in one step. This authoritative reference presents a number of recent contributions that help define the current art and science of MDLC. Topics covered include instrumentation, theory, methods development, and applications of MDLC in the life sciences and in industrial chemistry. With the information to help you perform very difficult separations of complex samples, this reference includes chapters contributed by leading experts or teams of experts.
Book Synopsis Capillary Electrophoresis–Mass Spectrometry for Metabolomics by : Rawi Ramautar
Download or read book Capillary Electrophoresis–Mass Spectrometry for Metabolomics written by Rawi Ramautar and published by Royal Society of Chemistry. This book was released on 2018-07-13 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis–mass spectrometry (CE-MS) has become a very useful analytical technique for the profiling of highly polar and charged metabolites in biological samples. In this book, the unique features of CE-MS for metabolomics studies are highlighted including CE separation modes, capillary coatings and practical aspects of CE-MS coupling alongside a comprehensive overview of recent technological developments and applications. CE-MS can be considered a relatively new technique in the field of metabolomics and it is therefore important to inform the scientific community about the possibilities of advanced CE-MS approaches for metabolomics studies. This book outlines the potential of this technique for researchers working in metabolomics, bioanalytics and biomarker analysis.
Book Synopsis Selectivity Enhancement in Capillary Electrophoresis - Development of a Two-dimensional Separation and a Dual Detection System by : Andrea Beutner
Download or read book Selectivity Enhancement in Capillary Electrophoresis - Development of a Two-dimensional Separation and a Dual Detection System written by Andrea Beutner and published by . This book was released on 2018 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Separation Techniques Applied to Omics Sciences by : Ana Valéria Colnaghi Simionato
Download or read book Separation Techniques Applied to Omics Sciences written by Ana Valéria Colnaghi Simionato and published by Springer Nature. This book was released on 2021-10-09 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.
Book Synopsis Development of Capillary Electrophoresis and Mass Spectrometry Methods in Protein Analysis by : Lin Liu
Download or read book Development of Capillary Electrophoresis and Mass Spectrometry Methods in Protein Analysis written by Lin Liu and published by . This book was released on 1999 with total page 242 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Book Synopsis Novel Micro-separation Techniques and Their Couplings to Mass Spectrometry for the Analysis of Complex Peptides and Proteins by :
Download or read book Novel Micro-separation Techniques and Their Couplings to Mass Spectrometry for the Analysis of Complex Peptides and Proteins written by and published by . This book was released on 2013 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mass spectrometry (MS)-based technology has evolved as a powerful tool for the analysis of a wide array of biomolecules. However, its application to complex peptidomics and proteomics samples, for example neuropeptides, has proven to be challenging due to their extreme chemical complexities and low abundances in biological tissue samples. This PhD dissertation is focused on addressing some of these challenges by developing novel micro-separation tools and coupling them with MS to achieve enhanced MS sensitivity and increased peptide and protein coverage with lower sample consumption and reduced experimental time. Both chromatography-based and electrophoresis-based separations as well as their couplings to MS have been explored in this work, including monolithic material-based chromatography, improved capillary electrophoresis (CE) separation and novel coupling of separations to mass spectrometric imaging. N-acryloxysuccinimide-based monolithic column has been fabricated with specific antibodies immobilized onto the column to enable the extraction of targeted neuropeptide family from biological matrices. Alternatively, enzymes were immobilized onto the column for highly efficient on-column enzymatic digestion for proteomics studies. A methacrylate-based monolithic column with C12 functional groups was developed and coupled to both electrospray ionization (ESI) MS and matrix-assisted laser desorption/ionization (MALDI) MS for reversed-phase liquid chromatography (RPLC). For capillary electrophoresis, two improved capillary isoelectric focusing (CIEF) systems have been developed, including membrane-assisted CIEF and immobilized pH-gradient CIEF to obtain reduced sample loss and enhanced MS signal for neuropeptide analysis. An improved sheathless pressure-assisted CE (PACE) system was also developed for coupling to MALDI MS. For novel separation-MS imaging coupling, three generations of interfaces have been designed to couple CE, LC and multi-dimensional separations to MS imaging (MSI) for quantitative analysis of complex peptides. Collectively, this work results in development of the new generation of micro-separation and sample preparation tools and their coupling to various mass spectrometric ionization and detection modes for sensitive and rapid analysis of complex peptides and proteins. The technological innovations have significantly expanded the toolbox for peptidomics/proteomics analysis and will enable more in-depth investigations on peptide function and regulation.
Book Synopsis Journal of Capillary Electrophoresis by :
Download or read book Journal of Capillary Electrophoresis written by and published by . This book was released on 2004 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis State-of-art Separation Mass Spectrometry in Protein Characterization by : Siyuan Liu
Download or read book State-of-art Separation Mass Spectrometry in Protein Characterization written by Siyuan Liu and published by . This book was released on 2016 with total page 223 pages. Available in PDF, EPUB and Kindle. Book excerpt: A therapeutic protein derived from recombinant DNA technology usually poses a high degree of complexity. A complete heterogeneity profile of the therapeutic protein is critical to ensure the quality, safety and efficacy of the drug. To achieve a complete mapping of all possible protein modifications including the various types of post-translational modifications and sequence variants, the incorporation of high resolution separation methods and high sensitivity detection techniques are required. The purpose of this dissertation is to develop and apply sensitive and robust mass spectrometry (MS)-based analytical platforms to address the challenges associated with protein identification and characterization. In the first chapter, the current analytical techniques, including various gel-based, chromatographic-based and capillary electrophoresis-based separation methods frequently incorporated with mass spectrometry for protein identification and characterization are reviewed. The traditional sample preparation workflows for MS-based protein identification are introduced. In addition, the application of mass spectrometry for the determination of protein post-translational modifications and the associated analytical challenges are discussed. The sample preparation steps involved in the LC-MS analysis of a therapeutic protein can potentially introduce disulfide scrambling leading to the incorrect characterization of the disulfide linkage structure. Chapter 2 focuses on the development of an LC-MS work flow for the determination of the disulfide mapping of proteins that are sensitive to disulfide scrambling. The recombinant human neublastin, a potential therapeutic agent with disulfide linkages partially elucidated via the traditional non-reduced multi-enzyme digestion strategy is studied. The modified workflow includes the optimization of the partial reduction and alkylation steps prior to multi-enzyme digestion in order to minimize the breakage of a relatively unstable intermolecular disulfide bond and the rapid thiol-disulfide exchange between adjacent cysteines. Using the developed work flow, while scrambling remained, complete and reproducible disulfide mapping was achieved via in-depth LC-MS analysis with CID and ETD fragmentations. In Chapter 3, a powerful methodology combining metabolic labeling, SDS-PAGE protein fractionation and nano LC-MS analysis is applied to isolate and identify the potential protein target to a novel small molecule drug from cell lysates. The non-covalent binding between the identified protein target and the drug is demonstrated with comprehensive characterization of the potential target. In Chapter 4, a new design of a low-sheath-flow liquid junction interface is described to integrate commercial capillary electrophoretic instrumentation and electrospray ionization mass spectrometer for automatic peptide and protein analysis. Different separation modes including capillary zone electrophoresis (CZE) and capillary isoelectric focusing (CIEF) were applied to couple with mass spectrometry via the liquid junction interface. Confident identification and high separation efficiency of a BSA digest are demonstrated with the integrated CZE-MS system. In addition, separation of a mixture of model proteins with closely related isoelectric points was achieved with CIEF-MS analysis leading to the identification of different types of post-translational modifications. The system was also employed to study the potential degradation products of a therapeutic protein, recombinant human growth hormone. The low-sheath-flow liquid junction interface integrated CIEF-ESI-MS system potentially can be an alternative technique to the well-established LC-MS method for the online enrichment and identification of the charge heterogeneity in biopharmaceuticals. Chapter 5 summarizes the work in the dissertation and provides a brief discussion on the future direction of each project.
Book Synopsis Two-dimensional Capillary-based Electrophoretic Separation Coupled to Mass Spectrometry by a Mechanical Valve by :
Download or read book Two-dimensional Capillary-based Electrophoretic Separation Coupled to Mass Spectrometry by a Mechanical Valve written by and published by . This book was released on 2017 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Unified Separation Science by : J. Calvin Giddings
Download or read book Unified Separation Science written by J. Calvin Giddings and published by Wiley-Interscience. This book was released on 1991-01-16 with total page 360 pages. Available in PDF, EPUB and Kindle. Book excerpt: Unifies the complex welter of techniques used for chemical separations by clearly formulating the concepts that are common to them. The mass transport phenomena underlying all separation processes are developed in a simple physical-mathematical form. The limitations and optimum performance of alternative separation techniques and the factors enhancing and limiting separation power can thus be described and explored. Generously illustrated and contains numerous exercises. Long awaited in the scientific community, it breaks new ground in understanding separation processes.
Book Synopsis Encyclopedia of Separation Science by : Ian D. Wilson
Download or read book Encyclopedia of Separation Science written by Ian D. Wilson and published by . This book was released on 2000 with total page 556 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 1 of this resource encyclopedia contains Level 1, which provides a broad overview of the theory of the 12 main categories of separation techniques. Volumes 2-4 (Level 2) expand coverage with detailed theoretical and technical descriptions of particular techniques. The remaining Volumes 5-9 (Level 3) cover applications of these techniques from the micro to the macro, and from the analytical laboratory bench to large-scale industrial processes. The last volume consists mainly of the index.
Book Synopsis Development and Application of Capillary Electrophoresis-electrospray Mass Spectrometry by :
Download or read book Development and Application of Capillary Electrophoresis-electrospray Mass Spectrometry written by and published by . This book was released on 2001 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Electrokinetic Phenomena by : Anurag Rathore
Download or read book Electrokinetic Phenomena written by Anurag Rathore and published by CRC Press. This book was released on 2003-09-16 with total page 491 pages. Available in PDF, EPUB and Kindle. Book excerpt: Electrokinetic Phenomena emphasizes the impact of methods such as capillary zone electrophoresis, capillary electrochromatography, and capillary gel electrophoresis on the analysis of biomolecules. This reference reveals the electrokinetic phenomena that underlie high-performance electro-based analytical tools and vividly depicts how electrodriven analytical tools revolutionize and expedite chemical, pharmaceutical, and biotechnological analysis. An authoritative overview, the book provides effective pathways for large-scale biomedical applications and describes how microfabricated and automated devices enhance and accelerate the analysis of biologically important molecules.
Download or read book Chemical Cytometry written by Chang Lu and published by Wiley-VCH. This book was released on 2010-03-08 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive introduction to the novel approach for biochemical analysis at the single cell level that covers recent methods for fractionation, detection and analysis of molecules from individual cells.
