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Developing Single Molecule Fluorescence Techniques And Their Application In The Dna Nanotechnology Field
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Book Synopsis Developing Single-molecule Fluorescence Techniques and Their Application in the DNA Nanotechnology Field by : Roman Tsukanov
Download or read book Developing Single-molecule Fluorescence Techniques and Their Application in the DNA Nanotechnology Field written by Roman Tsukanov and published by . This book was released on 2014 with total page 122 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies by : Amani Hariri
Download or read book Advancing DNA Nanotechnology Using Single Molecule Fluorescence Methodologies written by Amani Hariri and published by . This book was released on 2016 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "By taking it out of its biological role, DNA has served as a robust, reliable and programmable nanofabrication building block for the assembly of a variety of two- and three-dimensional discrete nanometer-sized structures with arbitrary shapes and designs. These molecularly addressable materials could serve as platforms for the site-specific hybridization with nanometer precision. Besides structural complexity, DNA objects evolved to comprise an intrinsic dynamic character allowing them to respond to surrounding stimuli in a predictable manner. Many researchers have previously constructed nanostructures using methods that rely on the spontaneous assembly of DNA in solution, in which case the more complex the assembly design is, the more error-prone the product becomes. This constitutes a major problem for the application of DNA-based structures in dynamic devices such as machines, motors, robots, and computers, where even small errors in assembly can drastically affect performance. Therefore, quantitative tools for analysing structures and dynamics of complex DNA nanostructures are critical. Single molecule fluorescence techniques have been transformative to the field of nanoscience and have played a leading role in providing a mature understanding and an informative feedback on the building blocks and final products of self-assembly. This thesis demonstrates how different single molecule fluorescence methodologies can be utilized to study and advance the structure, dynamics and integrity of DNA-assemblies, specifically those shaped as nanotubes. Conceptually, this research can be divided into four main parts: (i) synthesis optimization, (ii) structural characterization, (iii) monitoring dynamic structures, and (iv) studying structural dynamics. First, a solid-phase synthesis strategy and its visualization through single-molecule spectroscopy was devised to assemble DNA nanotubes in a stepwise fashion, with a full control over their size and sequence pattern. This method paves the way for the production of custom-made DNA nanotubes with fewer structural flaws than the spontaneous-assembly method. Second, single molecule photobleaching and two-color colocalization approaches were uniquely combined to provide a systematic way of assessing the polydispersity, stoichiometry and degree of defectiveness of different DNA nanotubes structures. These approaches will be of significant importance for many research groups synthesizing large supramolecular structures or studying naturally occurring ones, such as protein clusters, amyloids, etc. Third, in situ single molecule immobilization-based fluorescence microscopy was employed to introduce structural changes into DNA nanotubes by dynamically adjusting one or several of the edge lengths between the building blocks using a combination of strand displacement and loops. This is interesting for sensing applications, especially when the analyte produces large scale, detectable structural changes, and also for creating molecular muscles, capable of extension and contraction under external control. Lastly, dynamics and robustness of DNA nanotubes, reconfigured in response to site-specific deletion of DNA strands, were investigated using two color single molecule microscopy. This strategy enables to develop a better understanding of the collective structural changes within DNA structures in response to modifications in their repeat unit. Together, the different methods developed in this thesis underline the importance of single molecule techniques as powerful tools which can advance the field of DNA nanotechnology by enabling the production of well-defined high-quality objects that can meet the designer's compositional and dynamic specifications." --
Book Synopsis Single Molecules and Nanotechnology by : Rudolf Rigler
Download or read book Single Molecules and Nanotechnology written by Rudolf Rigler and published by Springer Science & Business Media. This book was released on 2007-12-07 with total page 322 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book focuses on recent advances in the rapidly evolving field of single molecule research. These advances are of importance for the investigation of biopolymers and cellular biochemical reactions, and are essential to the development of quantitative biology. Written by leading experts in the field, the articles cover a broad range of topics, including quantum photonics of organic dyes and inorganic nanoparticles and monitoring of single molecule (enzymatic) reactions.
Book Synopsis Fluorescence in Bio-inspired Nanotechnology by : Jonas Hannestad
Download or read book Fluorescence in Bio-inspired Nanotechnology written by Jonas Hannestad and published by Springer Science & Business Media. This book was released on 2013-07-20 with total page 127 pages. Available in PDF, EPUB and Kindle. Book excerpt: In his thesis Fluorescence in Bio-inspired Nanotechnology, Jonas Hannestad describes the evolving field of DNA nanotechnology in a lucid and easily accessible way. A central theme in the thesis is how biological structures and mechanisms constitute a basis for the design of novel technologies. Hannestad discusses how self-assembled, nanometer-scale DNA constructs can be functionalized using fluorescent labeling. In particular, he highlights how applications are based on fluorescence resonance energy transfer (FRET). Another important contribution is the development of a lipid monolayer platform for the step-by-step assembly of DNA nanoconstructs. The work in the thesis is based on five peer-reviewed papers published in high-profile journals, all of which involve major contributions from the author.
Book Synopsis Handbook of Single Molecule Fluorescence Spectroscopy by : Chris Gell
Download or read book Handbook of Single Molecule Fluorescence Spectroscopy written by Chris Gell and published by OUP Oxford. This book was released on 2006-08-17 with total page 278 pages. Available in PDF, EPUB and Kindle. Book excerpt: Analytical measurements at the single molecule level under ambient conditions have become almost routine in the past few years. The application of this technology to fundamental studies of heterogeneity in biomolecular structure and dynamics, chemical and biological reaction kinetics, and photophysics provides a rich playground for molecular scientists. The potential use of single molecule detection for nanotechnology and quantum information processing is a new and almost unexplored area. This handbook is intended for those interested in a practical introduction to single molecule investigations using fluorescence techniques and places special emphasis on the practicalities of achieving single molecule resolution, analysing the resulting data and exploration of the applications in biophysics. It is ideal for graduate research students and others embarking on work in this exciting field.
Book Synopsis Single-molecule Fluorescence Methods for DNA Nanostructure Assembly and Actuation by : Casey Platnich
Download or read book Single-molecule Fluorescence Methods for DNA Nanostructure Assembly and Actuation written by Casey Platnich and published by . This book was released on 2021 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: "DNA nanotechnology relies on the molecular recognition properties of DNA to produce complex architectures through self-assembly. DNA nanostructures allow scientists to organize functional materials with nanoscale precision, with applications spanning materials science, bioengineering, and physics. In addition to their remarkable performance as frameworks for other species, DNA constructs also possess intrinsic dynamic properties due to the non-covalent interactions that bring them together and show exceptional promise in the areas of controlled, personalized drug delivery and the development of biosensors.Although DNA nanostructures have become increasingly intricate and functional, the development of tools to study them has lagged. Historically, ensemble measurements have been primarily used to analyze the outputs of DNA nanotechnology, averaging the behaviour of billions of molecules within a sample to generate an output. This averaging means that the structure and performance of each individual construct is obscured, concealing malformed structures and masking properties associated with heterogeneity in size, length, and shape in self-assembled samples. For integration into engineered nanomaterials and biomedical devices, it is imperative that the DNA nanomaterials within a sample be structurally identical, with predictable and controllable behaviour. This goal requires the development of new analytical tools for the real-time observation of nanostructure assembly and actuation.This thesis describes the expansion of single-molecule fluorescence methodologies to probe the self-assembly pathways of DNA nanomaterials. These techniques monitor the self-assembly kinetics of surface-grafted DNA constructs, giving rise to a new generation of monodisperse, user-defined structures prepared using fully automated methods. The dynamic nature of these measurements also allows the single-molecule differentiation of the mechanisms of supramolecular polymerization. Exploring the fine-grained detail of self-assembly will enable the actualization of DNA nanostructures in custom delivery and sensing applications. In establishing new strategies to detect the assembly pathways of DNA architectures, this thesis provides a cohesive framework towards understanding hierarchical DNA assemblies at the single-molecule level"--
Book Synopsis Single Molecule Spectroscopy in Chemistry, Physics and Biology by : Astrid Graslund
Download or read book Single Molecule Spectroscopy in Chemistry, Physics and Biology written by Astrid Graslund and published by Springer Science & Business Media. This book was released on 2010-04-17 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the leading experts in the field, this book describes the development and current state of the art in single molecule spectroscopy. The application of this technique, which started 1989, in physics, chemistry and biosciences is displayed.
Book Synopsis Structural DNA Nanotechnology by : Nadrian C. Seeman
Download or read book Structural DNA Nanotechnology written by Nadrian C. Seeman and published by Cambridge University Press. This book was released on 2015 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: Written by the founder of the field, this is a comprehensive and accessible introduction to structural DNA nanotechnology.
Book Synopsis Fluorescence Spectroscopy in Biology by : Martin Hof
Download or read book Fluorescence Spectroscopy in Biology written by Martin Hof and published by Springer Science & Business Media. This book was released on 2004-12-08 with total page 330 pages. Available in PDF, EPUB and Kindle. Book excerpt: Volume 3 of this new series focuses on brandnew research and applications in biology, biophysics and other fields of life sciences. Many frontline researcher have contributed to this highly attractive and interdisciplinary volume which spans the entire field of present fluorescence spectroscopy including nanotechnology, membrane and DNA studies and fluorescence imaging in cancer research.
Author :Mario N. Berberan-Santos Publisher :Springer Science & Business Media ISBN 13 :3540739289 Total Pages :468 pages Book Rating :4.5/5 (47 download)
Book Synopsis Fluorescence of Supermolecules, Polymers, and Nanosystems by : Mario N. Berberan-Santos
Download or read book Fluorescence of Supermolecules, Polymers, and Nanosystems written by Mario N. Berberan-Santos and published by Springer Science & Business Media. This book was released on 2007-11-04 with total page 468 pages. Available in PDF, EPUB and Kindle. Book excerpt: This, the fourth volume in the Springer series on fluorescence, focuses on the fluorescence of nanosystems, polymers and supermolecules, as well as the development and application of fluorescent probes. Aimed at researchers in organic and physical chemistry and in material sciences, emphasis is placed on the fluorescence of artificial and biological nanosystems; single molecule fluorescence and the luminescence of polymers; and micro- and nanoparticles and nanotubes.
Book Synopsis Single Molecule Spectroscopy by : R. Rigler
Download or read book Single Molecule Spectroscopy written by R. Rigler and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 375 pages. Available in PDF, EPUB and Kindle. Book excerpt: The topics range from single molecule experiments in quantum optics and solid-state physics to analogous investigations in physical chemistry and biophysics.
Book Synopsis Single Molecule Tools, Part A: Fluorescence Based Approaches by :
Download or read book Single Molecule Tools, Part A: Fluorescence Based Approaches written by and published by Academic Press. This book was released on 2010-08-17 with total page 559 pages. Available in PDF, EPUB and Kindle. Book excerpt: Single molecule tools have begun to revolutionize the molecular sciences, from biophysics to chemistry to cell biology. They hold the promise to be able to directly observe previously unseen molecular heterogeneities, quantitatively dissect complex reaction kinetics, ultimately miniaturize enzyme assays, image components of spatially distributed samples, probe the mechanical properties of single molecules in their native environment, and "just look at the thing" as anticipated by the visionary Richard Feynman already half a century ago. Single Molecule Tools, Part A: Fluorescence Based Approaches captures a snapshot of this vibrant, rapidly expanding field, presenting articles from pioneers in the field intended to guide both the newcomer and the expert through the intricacies of getting single molecule tools. Includes time-tested core methods and new innovations applicable to any researcher employing single molecule tools Methods included are useful to both established researchers and newcomers to the field Relevant background and reference information given for procedures can be used as a guide to developing protocols in a number of disciplines
Book Synopsis Single-Molecule Tools for Bioanalysis by : Shuo Huang
Download or read book Single-Molecule Tools for Bioanalysis written by Shuo Huang and published by CRC Press. This book was released on 2022-05-25 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the last three decades, the fast development of single-molecule techniques has revolutionized the way we observe and understand biological processes. Some of these techniques have been further adapted as tools for bioanalysis. This book summarizes and details the frontiers of the development of these tools as well as their applications. The contributors are young and established researchers in their respective fields. The main content originates from the lecture notes of a chemistry graduate course taught by the book editor at Nanjing University. This book is suitable to be used as a textbook for a high-level undergraduate or an entry-level graduate course. The systematically written content provides a thorough illustration of the mechanisms of each methodology presented.
Book Synopsis Fluorescent Nanodiamonds by : Huan-Cheng Chang
Download or read book Fluorescent Nanodiamonds written by Huan-Cheng Chang and published by John Wiley & Sons. This book was released on 2018-09-11 with total page 296 pages. Available in PDF, EPUB and Kindle. Book excerpt: The most comprehensive reference on fluorescent nanodiamond physical and chemical properties and contemporary applications Fluorescent nanodiamonds (FNDs) have drawn a great deal of attention over the past several years, and their applications and development potential are proving to be manifold and vast. The first and only book of its kind, Fluorescent Nanodiamonds is a comprehensive guide to the basic science and technical information needed to fully understand the fundamentals of FNDs and their potential applications across an array of domains. In demonstrating the importance of FNDs in biological applications, the authors bring together all relevant chemistry, physics, materials science and biology. Nanodiamonds are produced by powerful cataclysmic events such as explosions, volcanic eruptions and meteorite impacts. They also can be created in the lab by high-pressure high-temperature treatment of graphite or detonating an explosive in a reactor vessel. A single imperfection can give a nanodiamond a specific, isolated color center which allows it to function as a single, trapped atom. Much smaller than the thickness of a human hair, a nanodiamond can have a huge surface area that allows it to bond with a variety of other materials. Because of their non-toxicity, nanodiamonds may be useful in biomedical applications, such as drug delivery and gene therapy. The most comprehensive reference on a topic of rapidly increasing interest among academic and industrial researchers across an array of fields Includes numerous case studies and practical examples from many areas of research and industrial applications, as well as fascinating and instructive historical perspectives Each chapter addresses, in-depth, a single integral topic including the fundamental properties, synthesis, mechanisms and functionalisation of FNDs The first book published by the key patent holder with his research group in the field of FNDs Fluorescent Nanodiamonds is an important working resource for a broad range of scientists and engineers in industry and academia. It will also be a welcome reference for instructors in chemistry, physics, materials science, biology and related fields.
Book Synopsis Development of Single Molecule Fluorescence Techniques with Application to Molecular Motors by : Lee Stirling Churchman
Download or read book Development of Single Molecule Fluorescence Techniques with Application to Molecular Motors written by Lee Stirling Churchman and published by . This book was released on 2007 with total page 96 pages. Available in PDF, EPUB and Kindle. Book excerpt: FIONA was employed to follow one motor domain of the dimeric myosin VI molecule. We found that myosin VI moves along actin using a "hand-over-hand" mechanism. SHREC was subsequently developed so that both motor domains of dimeric myosin V could be followed simultaneously. Myosin V was also observed to use a "hand-over-hand" mechanism. To study the kinetics of these dimeric myosins working concentrations approaching the Km of ATP binding to the myosin motor domain would be required (∼1 muM). Linear zero-mode waveguides (lZMW) were devised to reduce the background created by high concentrations of labeled ATP. The lZMW's reduce background by evanescent excitation, nanoconfinement, and limited fluorescence coupling out of the waveguide. Their linearity permits actin filaments to be included in the experimental design. As the collection of single molecule datasets employs specialized equipment and is time intensive, data analysis was optimized by statistical analysis to ensure that the most precise information was extracted.
Book Synopsis Single-molecule Techniques by : Paul R. Selvin
Download or read book Single-molecule Techniques written by Paul R. Selvin and published by CSHL Press. This book was released on 2008 with total page 511 pages. Available in PDF, EPUB and Kindle. Book excerpt: Geared towards research scientists in structural and molecular biology, biochemistry, and biophysics, this manual will be useful to all who are interested in observing, manipulating and elucidating the molecular mechanisms and discrete properties of macromolecules.
Book Synopsis Applied Fluorescence in Chemistry, Biology and Medicine by : Wolfgang Rettig
Download or read book Applied Fluorescence in Chemistry, Biology and Medicine written by Wolfgang Rettig and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 567 pages. Available in PDF, EPUB and Kindle. Book excerpt: This interdisciplinary book gives a comprehensive survey of the state-of-the-art: from applications and trends in fluorescence techniques in science to medicine and engineering. Written for practitioners and researchers in industry and academia, it covers fields like environmental and materials science, biology, medicine, physics and chemistry. Moreover, it reports on such new and breathtaking methods as ultra-fast time-resolved or single molecule spectroscopy, gives examples of applications in the fields of electroluminescent polymers, visualization of membrane potentials in neurons and fluorescence imaging of the brain.
