Contamination In Tissue Culture

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Contamination in Tissue Culture

Author : Jorgen Fogh
Publisher : Elsevier
ISBN 13 : 0323156983
Total Pages : 300 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Contamination in Tissue Culture by : Jorgen Fogh

Download or read book Contamination in Tissue Culture written by Jorgen Fogh and published by Elsevier. This book was released on 2012-12-02 with total page 300 pages. Available in PDF, EPUB and Kindle. Book excerpt: Contamination in Tissue Culture covers the sources, prevention, detection, and elimination of contamination in tissue culture. Composed of 12 chapters, the book describes the frequency of occurrence of contamination and the many different effects of contamination on cultured cells. After introducing the intraspecies contamination of cell cultures, the book explains a specific type of contamination, such as bacterial, fungal, viral, and parasitic contamination. A chapter in this book describes the reversible and irreversible alterations of cultured FL human amnion cells after experimental mycoplasmal infection. Chapters 9 and 10 examine the occurrence of tissue culture contaminants by electron microscopy and procedures for isolating and identifying viral contaminants. The concluding chapter covers sterility tests of media and solutions for tissue culture and the use of antibiotics. It also summarizes the major developments made as well as future challenges in the field. This book will be helpful to investigators, teachers, students, and technicians within the many disciplines of cell biology, physiology, cytology, virology, immunology, genetics, oncology, molecular biology, biochemistry, and biophysics, in which tissue and cell cultures are used, either as the primary object of research or as tools.

Micropropagation

Author : P. Debergh
Publisher : Springer Science & Business Media
ISBN 13 : 940092075X
Total Pages : 474 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Micropropagation by : P. Debergh

Download or read book Micropropagation written by P. Debergh and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 474 pages. Available in PDF, EPUB and Kindle. Book excerpt: Micropropagation is a technology that has developed within the past 30 years. Earlier overviews of plant tissue culture have reviewed micropropagation as just one of many tissue culture procedures in use. Since the applications of this technology have multiplied so rapidly in recent years, we decided that a specific overview of the technology was now appropriate Our book begins with a review of the general principles of tissue culture as applied to micropropagation. This review is concise since the general topic has been covered in numerous other books and reviews. The basic principles of laboratory design and construction are summarized in the second chapter. Common problems encountered in micropropagation, both during and after culture, are examined in detail in four chapters. As micropropagation developed from a laboratory curiosity to a commercial industry, different considerations became important. These are discussed in two chapters. An attempt has been made to assess the current status of commercial production around the world. This has been difficult because commercial production figures are often closely guarded and little has been done to collect statistics on this growing industry. Applications to a broad range of crops are discussed in a series of chapters. These try to report the state of the art in each area, but since applications for some crops are much more advanced than for others, the focus of these chapters varies depending upon the progress that has been made.

Cancer Cell Culture

Author : Simon P. Langdon
Publisher : Springer Science & Business Media
ISBN 13 : 1592594069
Total Pages : 346 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Cancer Cell Culture by : Simon P. Langdon

Download or read book Cancer Cell Culture written by Simon P. Langdon and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: The culture of cancer cells is routinely practiced in many academic research centers, biotechnology companies, and hospital laboratories. Cancer Cell Culture: Methods and Protocols describes easy-to-follow methods to guide both novice and more experienced researchers seeking to use new techniques in their laboratories. Our present understanding of the cell and molecular biology of cancer has been derived mainly from the use of cultured cancer cells and we cover a number of the most widely used assays to study function in current use. Part I introduces the basic concept of cancer cell culture and this is followed by a description of the general techniques used in many cell culture facilities. The importance of cell line characterization is now widely recognized and methods to characterize and authenticate cell lines are described in Part II. Part III covers the isolation and development of specific cancer cell types and provides valuable tips for those wishing to derive new cell line models. A wide range of procedures encompassing many of the key functional features of cancer cells are described in Part IV including assays to evaluate clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Methods to modify cancer cells are described in Part V, including protocols for transfection, development of drug-resistance, immortalization, and transfer in vivo. In Part VI methods of coculture of different cell types and contamination of cell lines are covered.

Pathogen and Microbial Contamination Management in Micropropagation

Author : Alan C. Cassells
Publisher : Springer Science & Business Media
ISBN 13 : 9401589518
Total Pages : 364 pages
Book Rating : 4.4/5 (15 download)

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Book Synopsis Pathogen and Microbial Contamination Management in Micropropagation by : Alan C. Cassells

Download or read book Pathogen and Microbial Contamination Management in Micropropagation written by Alan C. Cassells and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is based mainly on invited and offered papers presented at the Second International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures held at University College, Cork, Ireland in September 1996, with additional invited papers. The First International Symposium on Bacterial and Bacteria-like Contaminants of Plant Tissue Cultures was held at the same venue in 1987 and was published as Acta Horticulturae volume 225, 1988. In the intervening years there have been considerable advances in both plant disease diagnostics and in the development of structured approaches to the management of disease and microbial contamination in micropropagation. These approaches have centred on attempts to separate, spatially, the problems of disease transmission and laboratory contamination. Disease-control is best achieved by establishing pathogen-free cultures while laboratory contamination is based on subsequent good working practice. Control of losses due to pathogens and microbial contamination in vitro addresses, arguably, the most importance causes of losses in the industry; nevertheless, losses at and post establishment can also be considerable due to poor quality microplants or micro-shoots. In this symposium, a holistic approach to pathogen and microbial contamination control is evident with the recognition that micropropagators must address pathogen and microbial contamination in vitro, and diseases and microplant failure at establishment. There is increasing interest in establishing beneficial bacterial and mycorrhizal association with microplants in vitro and in vivo.

Atlas of Human Pluripotent Stem Cells in Culture

Author : Lyn Healy
Publisher : Springer
ISBN 13 : 1489975071
Total Pages : 216 pages
Book Rating : 4.4/5 (899 download)

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Book Synopsis Atlas of Human Pluripotent Stem Cells in Culture by : Lyn Healy

Download or read book Atlas of Human Pluripotent Stem Cells in Culture written by Lyn Healy and published by Springer. This book was released on 2014-11-07 with total page 216 pages. Available in PDF, EPUB and Kindle. Book excerpt: This lavishly-illustrated, authoritative atlas explores the intricate art of culturing human pluripotent stem cells. Twelve chapters – containing more than 280 color illustrations – cover a variety of topics in pluripotent stem cell culturing including mouse and human fibroblasts, human embryonic stem cells and induced pluripotent stem cells, characteristic staining patterns, and abnormal cultures, among others. Atlas of Human Pluripotent Stem Cells in Culture is a comprehensive collection of illustrated techniques complemented by informative and educational captions examining what good quality cells look like and how they behave in various environments. Examples of perfect cultures are compared side-by-side to less-than-perfect and unacceptable examples of human embryonic and induced pluripotent stem cell colonies. This detailed and thorough atlas is an invaluable resource for researchers, teachers, and students who are interested in or working with stem cell culturing.

Biomedical Tissue Culture

Author : Luca Ceccherini-Nelli
Publisher :
ISBN 13 : 9789535153337
Total Pages : 250 pages
Book Rating : 4.1/5 (533 download)

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Book Synopsis Biomedical Tissue Culture by : Luca Ceccherini-Nelli

Download or read book Biomedical Tissue Culture written by Luca Ceccherini-Nelli and published by . This book was released on 2012 with total page 250 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes many aspects of tissue culture models in an extensive manner. The book includes many topics like, the development of cultural methods to produce massive neuronal syncytial connections and induce their fusion with formation of bi- and multinucleated cells, the applicability in research of cell lines derived from the cultivation of placenta derived cells and cell populations with properties of progenitor/stem cells, the procedures that may be used to regenerate cartilage tissue with appropriate mechanical properties, the improvements made in the use of cell culture for virus isolation, tissue-based models for HCV replication in vitro, cultures to study the pathogenicity of enteropathogenic bacteria, the use of viral DNA and cDNA Array in the diagnosis of respiratory tract infections (RTI) in comparison with routine diagnosis methods.

Physiology, Growth and Development of Plants in Culture

Author : P.J. Lumsden
Publisher : Springer Science & Business Media
ISBN 13 : 9401107904
Total Pages : 439 pages
Book Rating : 4.4/5 (11 download)

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Book Synopsis Physiology, Growth and Development of Plants in Culture by : P.J. Lumsden

Download or read book Physiology, Growth and Development of Plants in Culture written by P.J. Lumsden and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 439 pages. Available in PDF, EPUB and Kindle. Book excerpt: Over recent years, progress in micropropagation has not been as rapid as many expected and, even now, relatively few crops are produced commercially. One reason for this is that the biology of material growing in vitro has been insufficiently understood for modifications to standard methods to be made based on sound physiological principles. However, during the past decade, tissue culture companies and others have invested considerable effort to reduce the empirical nature of the production process. The idea of the conference `Physiology, Growth and Development of Plants and Cells in Culture' (Lancaster, 1992) was to introduce specialists in different areas of plant physiology to micropropagators, with the express aims of disseminating as wide a range of information to as large a number of participants as possible, and beginning new discussions on the constraints and potentials affecting the development of in vitro plant production methods. This book is based on presentations from the conference and has been divided into two main sections, dealing with either aspects of the in vitro environment -- light, nutrients, water, gas -- or with applied aspects of the culture process -- morphogenesis, acclimation, rejuvenation, contamination.

Introduction to Cell and Tissue Culture

Author : Jennie P. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 0585275718
Total Pages : 247 pages
Book Rating : 4.5/5 (852 download)

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Book Synopsis Introduction to Cell and Tissue Culture by : Jennie P. Mather

Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Culture of Animal Cells

Author : R. Ian Freshney
Publisher : Wiley-Liss
ISBN 13 : 9780471589662
Total Pages : 510 pages
Book Rating : 4.5/5 (896 download)

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Book Synopsis Culture of Animal Cells by : R. Ian Freshney

Download or read book Culture of Animal Cells written by R. Ian Freshney and published by Wiley-Liss. This book was released on 1993-12-29 with total page 510 pages. Available in PDF, EPUB and Kindle. Book excerpt: This masterful third edition of Freshney's Culture of Animal Cells updates and considerably expands the scope of its predecessor and still enables both the novice and the experiences researcher to apply the basic and more sophisticated techniques of tissue culture. New Topics covered include: the use of molecular techniques in cell culture, such as DNA fingerprinting, fluorescence in situ hybridization, and chromosome painting cell interactions in cell culture new methods for separating cells new or refined methods for accessing cytotoxicity, viability, and mutagenicity experimental details for culture of specialized cells types not covered in previous editions new or refined techniques for visualizing clues, including time-lapse photography and confocal microscopy The revised and expanded third edition offers the following features: over 350 new reference to the primary literature an international list of cell banks an international listing of reagants and commercial supplies a subject index a glossary Also available: 0471169021 Culture of Animal Cells: A Multimedia Guide CD-ROM $150 est. From the reviews: "I strongly recommend this volume for any laboratory wishing to culture mammalian cells" - Biotechnology "It is not very often that it is possible to say of a book, 'I don't know how I managed without it previously.' Here is such a book" - Cell Biology International Reports

Biostimulation Remediation Technologies for Groundwater Contaminants

Author : Rathoure, Ashok K.
Publisher : IGI Global
ISBN 13 : 1522541632
Total Pages : 355 pages
Book Rating : 4.5/5 (225 download)

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Book Synopsis Biostimulation Remediation Technologies for Groundwater Contaminants by : Rathoure, Ashok K.

Download or read book Biostimulation Remediation Technologies for Groundwater Contaminants written by Rathoure, Ashok K. and published by IGI Global. This book was released on 2018-05-19 with total page 355 pages. Available in PDF, EPUB and Kindle. Book excerpt: The rapid progression of technology has significantly impacted population growth, urbanization, and industrialization in modern society. These developments, while positive on the surface, have created critical environmental problems in recent years. Biostimulation Remediation Technologies for Groundwater Contaminants is a critical scholarly publication that examines the release of heavy metals into the environment as a result of human activities and the use of nanoparticles and other technologies to manage and treat the effects of the pollution. Featuring coverage on a broad range of topics such as toxicity of heavy metals, bioremediation, and acclimated bacterial strains, this book is geared toward environmentalists, engineers, academics, researchers, and graduate-level students seeking current research on bioremediation as an alternate way to manage or degrade heavy metal waste.

Microorganisms in Plant Conservation and Biodiversity

Author : K. Sivasithamparam
Publisher : Springer Science & Business Media
ISBN 13 : 1402007809
Total Pages : 382 pages
Book Rating : 4.4/5 (2 download)

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Book Synopsis Microorganisms in Plant Conservation and Biodiversity by : K. Sivasithamparam

Download or read book Microorganisms in Plant Conservation and Biodiversity written by K. Sivasithamparam and published by Springer Science & Business Media. This book was released on 2002-07-31 with total page 382 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant conservation is increasingly recognised as an outstanding global priority, yet despite considerable efforts over the last few decades, the number of threatened species continues to rise. The practice of plant conservation has for too long been a rather hit-or-miss mixture of methods. While microorganisms have been recognised as a crucial and essential element in supporting the lifecycles of plant species, there has been limited recognition of the relationships between macro level conservation facilitating ecosystem functioning at the micro level. This book addresses the role of microorganisms in conservation - both their support functions and deleterious roles in ecosystem processes and species survival. Importantly, a number of authors highlight how microbial diversity is, itself, now under threat from the many and pervasive influences of man. What is clear from this volume is that like many contemporary treatments of plant and animal conservation, the solution to mitigate the erosion of biodiversity is not simple. This book represents an attempt to bring to the fore the ecological underwriting provided by microorganisms.

Plant Cell Culture Protocols

Author : Víctor M. Loyola-Vargas
Publisher : Springer Science & Business Media
ISBN 13 : 1592599591
Total Pages : 389 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Plant Cell Culture Protocols by : Víctor M. Loyola-Vargas

Download or read book Plant Cell Culture Protocols written by Víctor M. Loyola-Vargas and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: A comprehensive state-of-the-art collection of the most frequently used techniques for plant cell and tissue culture. Readily reproducible and extensively annotated, the methods range from general methodologies, such as culture induction, growth and viability evaluation, and contamination control, to such highly specialized techniques as chloroplast transformation involving the laborious process of protoplast isolation and culture. Most of the protocols are currently used in the research programs of the authors or represent important parts of business projects aimed at the generation of improved plant materials. Two new appendices explain the principles for formulating culture media and the composition of the eight most commonly used media formulations, and list more than 100 very useful internet sites.

Rapid Diagnosis of Mycoplasmas

Author : Itzahak Kahane
Publisher : Springer Science & Business Media
ISBN 13 : 1461524784
Total Pages : 240 pages
Book Rating : 4.4/5 (615 download)

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Book Synopsis Rapid Diagnosis of Mycoplasmas by : Itzahak Kahane

Download or read book Rapid Diagnosis of Mycoplasmas written by Itzahak Kahane and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 240 pages. Available in PDF, EPUB and Kindle. Book excerpt: This compendium is the result of the FEMS Workshop on "Rapid Diagnosis of Mycoplasmas" which I organized and which took place in Jerusalem, Israel, August 11-23, 1991. The first week's sessions were held at a resort on the outskirts of Jerusalem and consisted of lectures and discussions. This part was modelled along the lines of the Gordon Conference in the USA, i.e., in an intimate atmo sphere in which everyone could mix and exchange ideas, and was very benefi cial. About 100 scientists from around the world attended the first week. Dur ing the first week, the biology, molecular biology and pathophysiology of myco plasmas, as well as all the main diagnostic methods were covered, including both conventional and the newer technologies. The session on mycoplasmas in the human urogenital tracts was held in conjunction with the Israel Society for the Study and Prevention of Sexually Transmitted Disease. The second week was a laboratory session and was held at the Hebrew University-Hadassah Medical School campus in Ein Karem, Jerusalem. All ex periments were conducted by eminent specialists in their field. The lab session had 36 participants from 19 countries who used the most modern techniques for the diagnosis of mycoplasmas in medicine, veterinary medicine and agri culture. The efficacy of several commercial kits were also tested at this time. I want to again thank everyone who helped and supported this work shop, as well as the authors of the various chapters.

Molecular Biology of The Cell

Author : Bruce Alberts
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of The Cell by : Bruce Alberts

Download or read book Molecular Biology of The Cell written by Bruce Alberts and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Animal Cell Culture

Author : John M. Davis
Publisher : John Wiley & Sons
ISBN 13 : 0470666587
Total Pages : 389 pages
Book Rating : 4.4/5 (76 download)

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Book Synopsis Animal Cell Culture by : John M. Davis

Download or read book Animal Cell Culture written by John M. Davis and published by John Wiley & Sons. This book was released on 2011-03-21 with total page 389 pages. Available in PDF, EPUB and Kindle. Book excerpt: This is a comprehensive research guide that describes both the key new techniques and more established methods. Every chapter discusses the merits and limitations of the various approaches and then provides selected tried-and-tested protocols, as well as a plethora of good practical advice, for immediate use at the bench. It presents the most accessible and comprehensive introduction available to the culture and experimental manipulation of animal cells. Detailed protocols for a wide variety of methods provide the core of each chapter, making new methodology easily accessible. This book is an essential laboratory manual for all undergraduates and graduates about to embark on a cell culture project. It is a book which both experienced researchers and those new to the field will find invaluable.

Modern Applications of Plant Biotechnology in Pharmaceutical Sciences

Author : Saurabh Bhatia
Publisher : Academic Press
ISBN 13 : 0128024984
Total Pages : 452 pages
Book Rating : 4.1/5 (28 download)

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Book Synopsis Modern Applications of Plant Biotechnology in Pharmaceutical Sciences by : Saurabh Bhatia

Download or read book Modern Applications of Plant Biotechnology in Pharmaceutical Sciences written by Saurabh Bhatia and published by Academic Press. This book was released on 2015-07-22 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Modern Applications of Plant Biotechnology in Pharmaceutical Sciences explores advanced techniques in plant biotechnology, their applications to pharmaceutical sciences, and how these methods can lead to more effective, safe, and affordable drugs. The book covers modern approaches in a practical, step-by-step manner, and includes illustrations, examples, and case studies to enhance understanding. Key topics include plant-made pharmaceuticals, classical and non-classical techniques for secondary metabolite production in plant cell culture and their relevance to pharmaceutical science, edible vaccines, novel delivery systems for plant-based products, international industry regulatory guidelines, and more. Readers will find the book to be a comprehensive and valuable resource for the study of modern plant biotechnology approaches and their pharmaceutical applications. Builds upon the basic concepts of cell and plant tissue culture and recombinant DNA technology to better illustrate the modern and potential applications of plant biotechnology to the pharmaceutical sciences Provides detailed yet practical coverage of complex techniques, such as micropropogation, gene transfer, and biosynthesis Examines critical issues of international importance and offers real-life examples and potential solutions

Plant Cell and Tissue Culture - A Tool in Biotechnology

Author : Karl-Hermann Neumann
Publisher : Springer Science & Business Media
ISBN 13 : 3540938834
Total Pages : 332 pages
Book Rating : 4.5/5 (49 download)

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Book Synopsis Plant Cell and Tissue Culture - A Tool in Biotechnology by : Karl-Hermann Neumann

Download or read book Plant Cell and Tissue Culture - A Tool in Biotechnology written by Karl-Hermann Neumann and published by Springer Science & Business Media. This book was released on 2009-04-28 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about.