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Chemistry For Probing Protein Protein Interactions
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Book Synopsis Chemistry for Probing Protein-protein Interactions by :
Download or read book Chemistry for Probing Protein-protein Interactions written by and published by . This book was released on 2012 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt: One of the most important tasks facing modern organic chemists is the development of new approaches to study the organic chemistry of living systems. One key aspect of this charge is to devise methods to probe and perturb the dynamic processes which are mediated by proteins. This thesis presents two distinct chemistry-based approaches for interrogating protein systems. Chemical approaches to protein modification can be used to install biophysical probes into proteins. We proposed to develop a bioconjugation reaction that could be used to modify proteins at a single residue. We recognized that L-pyrrolysine contained an electrophilic pyrroline moiety and, therefore, could be used as a reaction substrate for a chemoselective bioconjugation reaction. We have developed an efficient synthesis of pyrrolysine and generated pyrrolysine-containing proteins using the synthetically prepared amino acid. We explored its reactivity to small molecules containing activated esters. We discovered that, although pyrrolysine is reactive to nucleophiles, its ability to chemoselectively form stable adducts with a single reagent is elusive. By tuning the reactivity of the labeling reagent, we have identified a potential new class of pyrroline-reactive compounds. A second approach to characterizing protein systems is to introduce highly specific probes that interact with the protein complexes. We have prepared synthetic multivalent ligands to probe the organization of transmembrane protein clusters that Escherichia coli use to control their movement. The multivalent display of receptor-binding epitopes target specific receptors. A covalently-bound marker on the multivalent ligand can facilitate imaging of ligand-bound states of receptor clusters. Additionally, because whole cells can be difficult to image due to sample size, we have reconstituted the transmembrane proteins with their signaling partners in small vesicles and shown that they adopt native-like protein assemblies. We anticipate that the combined approach of multivalent reporter probes and reconstituted protein complexes in native membranes will lead to a molecular understanding of multi-protein oligomerization. Chemistry-based methods offer a unique approach for modifying protein structure while minimizing functional perturbations. By understanding chemical reactivity, the ability to synthesize large molecules and interrogate large protein complexes is not limited by the type, size or complexity of the materials at hand.
Book Synopsis Probing Protein-protein Interactions with Peptide Libraries and Arrays by :
Download or read book Probing Protein-protein Interactions with Peptide Libraries and Arrays written by and published by . This book was released on 2008 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Dissecting Protein Assembly by : Wei-Feng Xue
Download or read book Dissecting Protein Assembly written by Wei-Feng Xue and published by . This book was released on 2005 with total page 46 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein'Protein Interactions by : Haian Fu
Download or read book Protein'Protein Interactions written by Haian Fu and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 525 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the mysteries stored in our DNA have been more completely revealed, scientists have begun to face the extraordinary challenge of unraveling the int- cate network of protein–protein interactions established by that DNA fra- work. It is increasingly clear that proteins continuously interact with one another in a highly regulated fashion to determine cell fate, such as proliferation, diff- entiation, or death. These protein–protein interactions enable and exert str- gent control over DNA replication, RNA transcription, protein translation, macromolecular assembly and degradation, and signal transduction; essentially all cellular functions involve protein–protein interactions. Thus, protein–p- tein interactions are fundamental for normal physiology in all organisms. Alt- ation of critical protein–protein interactions is thought to be involved in the development of many diseases, such as neurodegenerative disorders, cancers, and infectious diseases. Therefore, examination of when and how protein–p- tein interactions occur and how they are controlled is essential for understa- ing diverse biological processes as well as for elucidating the molecular basis of diseases and identifying potential targets for therapeutic interventions. Over the years, many innovative biochemical, biophysical, genetic, and computational approaches have been developed to detect and analyze p- tein–protein interactions. This multitude of techniques is mandated by the diversity of physical and chemical properties of proteins and the sensitivity of protein–protein interactions to cellular conditions.
Book Synopsis Biological and Biomedical Infrared Spectroscopy by : Andreas Barth
Download or read book Biological and Biomedical Infrared Spectroscopy written by Andreas Barth and published by IOS Press. This book was released on 2009 with total page 448 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although infrared spectroscopy has been applied with success to the study of important biological and biomedical processes for many years, key advances in this vibrant technique have led to its increasing use, ranging from characterization of individual macromolecules (DNA, RNA, lipids, proteins) to human tissues, cells and their components. Infrared spectroscopy thus has a significant role to play in the analysis of the vast number of genes and proteins being identified by the various genomic sequencing projects. Whilst this book gives an overview of the field, it highlights more recent developments, such as the use of bright synchrotron radiation for recording infrared spectra, the development of two-dimensional infrared spectroscopy and the ability to record infrared spectra at ultra fast speeds.
Book Synopsis Proteomics and Protein-Protein Interactions by : Gabriel Waksman
Download or read book Proteomics and Protein-Protein Interactions written by Gabriel Waksman and published by Springer Science & Business Media. This book was released on 2006-12-22 with total page 325 pages. Available in PDF, EPUB and Kindle. Book excerpt: Gabriel Waksman Institute of Structural Molecular Biology, Birkbeck and University College London, Malet Street, London WC1E 7HX, United Kingdom Address for correspondence: Professor Gabriel Waksman Institute of Structural Molecular Biology Birkbeck and University College London Malet Street London WC1E 7H United Kingdom Email: g. waksman@bbk. ac. uk and g. waksman@ucl. ac. uk Phone: (+44) (0) 207 631 6833 Fax: (+44) (0) 207 631 6833 URL: http://people. cryst. bbk. ac. uk/?ubcg54a Gabriel Waksman is Professor of Structural Molecular Biology at the Institute of Structural Molecular Biology at UCL/Birkbeck, of which he is also the director. Before joining the faculty of UCL and Birkbeck, he was the Roy and Diana Vagelos Professor of Biochemistry and Molecular Biophysics at the Washington University School of Medicine in St Louis (USA). The rapidly evolving ?eld of protein science has now come to realize the ubiquity and importance of protein–protein interactions. It had been known for some time that proteins may interact with each other to form functional complexes, but it was thought to be the property of only a handful of key proteins. However, with the advent of hi- throughput proteomics to monitor protein–protein interactions at an organism level, we can now safely state that protein–protein interactions are the norm and not the exception.
Book Synopsis Computational Protein-Protein Interactions by : Ruth Nussinov
Download or read book Computational Protein-Protein Interactions written by Ruth Nussinov and published by CRC Press. This book was released on 2009-06-26 with total page 346 pages. Available in PDF, EPUB and Kindle. Book excerpt: Often considered the workhorse of the cellular machinery, proteins are responsible for functions ranging from molecular motors to signaling. The broad recognition of their involvement in all cellular processes has led to focused efforts to predict their functions from sequences, and if available, from their structures. An overview of current resear
Book Synopsis Activity-Based Protein Profiling by : Benjamin F. Cravatt
Download or read book Activity-Based Protein Profiling written by Benjamin F. Cravatt and published by Springer. This book was released on 2019-01-25 with total page 417 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides a collection of contemporary perspectives on using activity-based protein profiling (ABPP) for biological discoveries in protein science, microbiology, and immunology. A common theme throughout is the special utility of ABPP to interrogate protein function and small-molecule interactions on a global scale in native biological systems. Each chapter showcases distinct advantages of ABPP applied to diverse protein classes and biological systems. As such, the book offers readers valuable insights into the basic principles of ABPP technology and how to apply this approach to biological questions ranging from the study of post-translational modifications to targeting bacterial effectors in host-pathogen interactions.
Book Synopsis Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification by : Jianpeng Wang
Download or read book Study of the Peptide-Peptide and Peptide-Protein Interactions and Their Applications in Cell Imaging and Nanoparticle Surface Modification written by Jianpeng Wang and published by Springer. This book was released on 2016-09-26 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: This thesis focuses on the study of interactions between protein and peptides and their potential applications in cell imaging and nanoparticle surface modification. Drawing inspiration from naturally occurring coiled-coil binding pairs, it proposes a novel covalent peptide tag and probe system, based on the concept of “affinity guided covalent conjugation.” This newly established methodology provides complementary resolution to protein labeling, imaging and trafficking. By systematically investigating the coordination interaction between protein and quantum dots using various engineered protein ligands, this thesis proposes a general rule for protein self-assembly on the surface of quantum dots and reports a revolutionized nanobelt protein in accordance with this rule. It is an extraordinary example of interdisciplinary research, providing answers to real-life biological problems from a chemistry perspective.
Book Synopsis Molecular Probing of Local Protein-protein Interactions by : Maria Österlund
Download or read book Molecular Probing of Local Protein-protein Interactions written by Maria Österlund and published by . This book was released on 2001 with total page 49 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Host-Guest Molecular Interactions by : Derek J. Chadwick
Download or read book Host-Guest Molecular Interactions written by Derek J. Chadwick and published by John Wiley & Sons. This book was released on 2008-04-30 with total page 288 pages. Available in PDF, EPUB and Kindle. Book excerpt: Composed of contributions from experts in the chemical and biological sciences, it explores host-guest molecular interactions leading to the formation of molecular assemblies containing two or more species. Exciting applications are emerging in this field and it is expected that improved understanding of the interactions in synthetic host molecule complexes will lead to a better understanding of the more complex biological systems. Topics include biomimetic chemistry, preorganization, self-assembly, template-directed synthesis, antibiotic binding to peptides and DNA, interactions between proteins and other molecules.
Book Synopsis Protein–Protein Interaction Regulators by : Siddhartha Roy
Download or read book Protein–Protein Interaction Regulators written by Siddhartha Roy and published by Royal Society of Chemistry. This book was released on 2020-12-14 with total page 399 pages. Available in PDF, EPUB and Kindle. Book excerpt: New genomic information has revealed the crucial role that protein–protein interactions (PPIs) play in regulating numerous cellular functions. Aberrant forms of these interactions are common in numerous diseases and thus PPIs have emerged as a vast class of critical drug targets. Despite the importance of PPIs in biology, it has been extremely challenging to convert targets into therapeutics and targeting PPIs had long been considered a very difficult task. However, over the past decade the field has advanced with increasing growth in the number of successful PPI regulators. Protein–Protein Interaction Regulators surveys the latest advances in the structural understanding of PPIs as well as recent developments in modulator discovery.
Book Synopsis Disruption of Protein-Protein Interfaces by : Stefano Mangani
Download or read book Disruption of Protein-Protein Interfaces written by Stefano Mangani and published by Springer Science & Business Media. This book was released on 2013-06-28 with total page 167 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Disruption of Protein-Protein Interfaces" reviews the latest developments and future perspectives in drug discovery at protein-protein interfaces. The authors detail experimental and computational tools to tackle the subject and highlight the contribution of the Italian research community to the field. Evidence shows that blocking or modulating protein-protein interactions might lead to the development of useful new drugs. Consequently, in recent years great effort has been dedicated to unveiling the molecular details of protein-protein interfaces by structural techniques e.g. X-ray diffraction, NMR spectroscopy. This book, written and edited by leaders in the field, provides examples from the literature of successes and failures to develop drug-like molecules effective in interacting at protein-protein interfaces.
Book Synopsis Affinity-guided Chemical Probes for the Study of Protein Interactions by : Hester Annie Beard
Download or read book Affinity-guided Chemical Probes for the Study of Protein Interactions written by Hester Annie Beard and published by . This book was released on 2018 with total page 352 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Protein-Protein Interactions by : Weibo Cai
Download or read book Protein-Protein Interactions written by Weibo Cai and published by BoD – Books on Demand. This book was released on 2012-03-30 with total page 488 pages. Available in PDF, EPUB and Kindle. Book excerpt: Proteins are indispensable players in virtually all biological events. The functions of proteins are coordinated through intricate regulatory networks of transient protein-protein interactions (PPIs). To predict and/or study PPIs, a wide variety of techniques have been developed over the last several decades. Many in vitro and in vivo assays have been implemented to explore the mechanism of these ubiquitous interactions. However, despite significant advances in these experimental approaches, many limitations exist such as false-positives/false-negatives, difficulty in obtaining crystal structures of proteins, challenges in the detection of transient PPI, among others. To overcome these limitations, many computational approaches have been developed which are becoming increasingly widely used to facilitate the investigation of PPIs. This book has gathered an ensemble of experts in the field, in 22 chapters, which have been broadly categorized into Computational Approaches, Experimental Approaches, and Others.
Book Synopsis Probing Protein-protein Interactions by Mass Spectrometry by : Tatiana Pimenova
Download or read book Probing Protein-protein Interactions by Mass Spectrometry written by Tatiana Pimenova and published by . This book was released on 2009 with total page 147 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Inhibitors of Protein–Protein Interactions by : Ali Tavassoli
Download or read book Inhibitors of Protein–Protein Interactions written by Ali Tavassoli and published by Royal Society of Chemistry. This book was released on 2020-12-07 with total page 357 pages. Available in PDF, EPUB and Kindle. Book excerpt: Protein-protein interactions (PPI) are at the heart of the majority of cellular processes, and are frequently dysregulated or usurped in disease. Given this central role, the inhibition of PPIs has been of significant interest as a means of treating a wide variety of diseases. However, there are inherent challenges in developing molecules capable of disrupting the relatively featureless and large interfacial areas involved. Despite this, there have been a number of successes in this field in recent years using both traditional drug discovery approaches and innovative, interdisciplinary strategies using novel chemical scaffolds. This book comprehensively covers the various aspects of PPI inhibition, encompassing small molecules, peptidomimetics, cyclic peptides, stapled peptides and macrocycles. Illustrated throughout with successful case studies, this book provides a holistic, cutting-edge view of the subject area and is ideal for chemical biologists and medicinal chemists interested in developing PPI inhibitors.
