Cell Tissue Culture

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Introduction to Cell and Tissue Culture

Author : Jennie P. Mather
Publisher : Springer Science & Business Media
ISBN 13 : 0585275718
Total Pages : 247 pages
Book Rating : 4.5/5 (852 download)

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Book Synopsis Introduction to Cell and Tissue Culture by : Jennie P. Mather

Download or read book Introduction to Cell and Tissue Culture written by Jennie P. Mather and published by Springer Science & Business Media. This book was released on 2007-08-20 with total page 247 pages. Available in PDF, EPUB and Kindle. Book excerpt: It is a pleasure to contribute the foreword to Introduction to Cell and Tissue Culture: The ory and Techniques by Mather and Roberts. Despite the occasional appearance of thought ful works devoted to elementary or advanced cell culture methodology, a place remains for a comprehensive and definitive volume that can be used to advantage by both the novice and the expert in the field. In this book, Mather and Roberts present the relevant method ology within a conceptual framework of cell biology, genetics, nutrition, endocrinology, and physiology that renders technical cell culture information in a comprehensive, logical for mat. This allows topics to be presented with an emphasis on troubleshooting problems from a basis of understanding the underlying theory. The material is presented in a way that is adaptable to student use in formal courses; it also should be functional when used on a daily basis by professional cell culturists in a- demia and industry. The volume includes references to relevant Internet sites and other use ful sources of information. In addition to the fundamentals, attention is also given to mod ern applications and approaches to cell culture derivation, medium formulation, culture scale-up, and biotechnology, presented by scientists who are pioneers in these areas. With this volume, it should be possible to establish and maintain a cell culture laboratory devot ed to any of the many disciplines to which cell culture methodology is applicable.

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Plant Cell, Tissue and Organ Culture

Author : Oluf Gamborg
Publisher : Springer Science & Business Media
ISBN 13 : 3642790488
Total Pages : 367 pages
Book Rating : 4.6/5 (427 download)

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Book Synopsis Plant Cell, Tissue and Organ Culture by : Oluf Gamborg

Download or read book Plant Cell, Tissue and Organ Culture written by Oluf Gamborg and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 367 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides all relevant protocols for basic and applied plant cell and molecular technologies, such as histology, electron microscopy, cytology, virus diagnosis, gene transfer and PCR. Also included are chapters on laboratory facilities, operation and management as well as a glossary and all the information needed to set up and carry out any of the procedures without having to use other resource books. It is especially designed for professionals and advanced students who wish to acquire practical skills and first-hand experience in plant biotechnology.

Plant Cell and Tissue Culture

Author : Indra K. Vasil
Publisher : Springer Science & Business Media
ISBN 13 : 9401726817
Total Pages : 593 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Plant Cell and Tissue Culture by : Indra K. Vasil

Download or read book Plant Cell and Tissue Culture written by Indra K. Vasil and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 593 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Cell and Tissue Culture gives an exhaustive account of plant cell culture and genetic transformation, including detailed chapters on all major field and plantation crops. Part A presents a comprehensive coverage of all necessary laboratory techniques for the initiation, nutrition, maintenance and storage of plant cell and tissue cultures, including discussions on these topics, as well as on morphogenesis and regeneration, meristem and shoot tip culture, plant protoplasts, mutant cell lines, variation in tissue cultures, isogenic lines, fertilization control, cryopreservation, transformation, and the production of secondary metabolites. Part B then proceeds into detail on the specific in vitro culture of specific crops, including cereals, legumes, vegetables, potatoes, other roots and tubers, oilseeds, temperate fruits, tropical fruits, plantation crops, forest trees and ornamentals. Plant Cell and Tissue Culture is, and is likely to remain, the laboratory manual of choice, as well as a source of inspiration and a guide to all workers in the field.

Tissue Culture Techniques

Author : Bernice M. Martin
Publisher : Springer Science & Business Media
ISBN 13 : 1461202477
Total Pages : 251 pages
Book Rating : 4.4/5 (612 download)

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Book Synopsis Tissue Culture Techniques by : Bernice M. Martin

Download or read book Tissue Culture Techniques written by Bernice M. Martin and published by Springer Science & Business Media. This book was released on 2013-12-01 with total page 251 pages. Available in PDF, EPUB and Kindle. Book excerpt: ACKNOWLEDGMENTS . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . Xl II INTRODUCTION . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . I STERILITY . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Aseptic Technique . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 5 Physical manipulations • Use of the sterile cabinet (hood) Sterilization Methods . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 14 Heat • Radiation • Toxic gas • Filtration • Antibiotics Quality Control of Sterilization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 23 Routine labeling Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 25 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 26 vi CONTENTS ROUTINE CELL CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . . . 29 Feeding Schedules and Media Components . . . . . . . . . . . . . . . . . . . . 29 General properties of media and salt solutions • water as a reagent· Establishingfeeding schedules Subcultivation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 46 Solutions and methods for adherent cells • Common enzyme solutions • Inoculating (seeding) the cultures Cell Enumeration and Cell Viability . . . . . . . . . . . . . . . . . . . . . . . . . . 54 Hemocytometer • Particle counter • Cell viability Putting Routine Methods to Work . . . . . . . . . . . . . . . . . . . . . . . . . . . 63 Normal cell growth characteristics Detecting and Disposing of Contamination . . . . . . . . . . . . . . . . . . . . 66 Bacteria and fUngi • Fungi • Mycoplasma • Viruses • Dealing with contamination Troubleshooting . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 73 Inadequate cell growth • Recurrent contamination • When to call your vendor Safety . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 80 Biological hazards • Chemical hazards Suggested Readings . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 85 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 89 EXPERIMENTS IN CULTURE . . . . . . . . . . . . . . . . . . . . . . . . . . 91 II Alterations of the Media . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 91 Serum • Treatments of serum • Plasma-derived serum • Serum-free and low-protein media Substrata. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 101 Coatingplasticware with solutions • Alterations with polymers • Using cells to coat the plasticware • Culturing cells on microcarriers Altering the Environment. . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 106 Temperature changes • Gaseous changes Problem Set . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 Exercises . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 110 CONTENTS vii PRIMARY CELL CULTURE. . . . . . . . . . . . . . . . . . . . . . . . . . . . 113 Isolation . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . 114 Dissection • Enzymatic dissociation methods • Nonenzymatic isolation • Purification of cell suspensions • Consideringyield and survival Chatacterization . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . . .

Cell and Tissue Culture for Medical Research

Author : Alan Doyle
Publisher :
ISBN 13 :
Total Pages : 482 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Cell and Tissue Culture for Medical Research by : Alan Doyle

Download or read book Cell and Tissue Culture for Medical Research written by Alan Doyle and published by . This book was released on 2000-08-22 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Cell and tissue culture is a technique in which plant or animal cells are grown under controlled conditions in the laboratory. This is then used for the analysis of the cells themselves, the assessment of the cell's response to chemicals, or as a tool to produce cellular-derived protein products. This book is a collection of fundamental and specific applied procedures in cell and tissue culture which form the basis of the new medical techniques of tissue engineering and gene therapy. It combines both detailed laboratory procedures and informative overviews. * Provides step-by-step protocols with troubleshooting tips and notes on time considerations. * Main procedures are supplemented by alternative procedures, background information and references. * Experimental examples indicate expected results.

Plant Cell and Tissue Culture - A Tool in Biotechnology

Author : Karl-Hermann Neumann
Publisher : Springer Science & Business Media
ISBN 13 : 3540938834
Total Pages : 332 pages
Book Rating : 4.5/5 (49 download)

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Book Synopsis Plant Cell and Tissue Culture - A Tool in Biotechnology by : Karl-Hermann Neumann

Download or read book Plant Cell and Tissue Culture - A Tool in Biotechnology written by Karl-Hermann Neumann and published by Springer Science & Business Media. This book was released on 2009-04-28 with total page 332 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book provides a general introduction as well as a selected survey of key advances in the fascinating field of plant cell and tissue culture as a tool in biotechnology. After a detailed description of the various basic techniques employed in leading laboratories worldwide, follows an extended account of important applications in, for example, plant propagation, secondary metabolite production and gene technology. Additionally, some chapters are devoted to historical developments in this domain, metabolic aspects, nutrition, growth regulators, differentiation and the development of culture systems. The book will prove useful to both newcomers and specialists, and even “old hands” in tissue culture should find some challenging ideas to think about.

Basic Cell Culture Protocols

Author : Jeffrey W. Pollard
Publisher : Springer Science & Business Media
ISBN 13 : 0896034410
Total Pages : 477 pages
Book Rating : 4.8/5 (96 download)

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Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard

Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.

Plant Cell and Tissue Culture

Author : J. Reinert
Publisher : Springer Science & Business Media
ISBN 13 : 364281784X
Total Pages : 85 pages
Book Rating : 4.6/5 (428 download)

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Book Synopsis Plant Cell and Tissue Culture by : J. Reinert

Download or read book Plant Cell and Tissue Culture written by J. Reinert and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 85 pages. Available in PDF, EPUB and Kindle. Book excerpt: The techniques of plant organ, tissue, and cell culture concentrated on reproducibility, simplicity and accu are now established in many research laboratories racy with sufficient illustration to make all mani throughout the world and are being used in numerous pulations clear. areas of plant science. Methods have been developed The drawings of items used in the bench layout to propagate plants and free them from viruses using diagrams are symbolic and are 'keyed in' by number to shoot tip culture. The regeneration of plants from callus the list of materials and equipment. A line around an culture has also proved useful commercially. Elegant item indicates that is sterile. techniques have been used to synthesise somatic The adoption of an integrated text in which diagrams hybrids by the fusion of protoplasts and to transform are related spatially to the methods will, we hope, help cells. These and many other techniques have been the student to grasp the techniques quickly and effec and can be used to investigate a variety of botanical tively. This is first and foremost a manual which has its phenomena as well as to improve crop plants and now place on the laboratory bench open in front of the provide an important part of the basic experimental student, a book to be used! skills required by a majority of experimental botanists.

Tissue Culture

Author : Paul F. Jr. Kruse
Publisher : Elsevier
ISBN 13 : 0323142079
Total Pages : 897 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Tissue Culture by : Paul F. Jr. Kruse

Download or read book Tissue Culture written by Paul F. Jr. Kruse and published by Elsevier. This book was released on 2012-12-02 with total page 897 pages. Available in PDF, EPUB and Kindle. Book excerpt: Tissue Culture: Methods and Applications presents an overview of the procedures for working with cells in culture and for using them in a wide variety of scientific disciplines. The book discusses primary tissue dissociation; the preparation of primary cultures; cell harvesting; and replicate culture methods. The text also describes protocols on single cell isolations and cloning; perfusion and mass culture techniques; cell propagation on miscellaneous culture supports; and the evaluation of culture dynamics. The recent techniques facilitating microscopic observation of cells; cell hybridization; and virus propagation and assay are also encompassed. The book further tackles the production of hormones and intercellular substances; the diagnosis and understanding of disease; as well as quality control measures. Scientists and professionals interested in methodology per se will find the book invaluable.

Plant Tissue and Cell Culture

Author : Herbert Edward Street
Publisher : Univ of California Press
ISBN 13 : 9780520024113
Total Pages : 520 pages
Book Rating : 4.0/5 (241 download)

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Book Synopsis Plant Tissue and Cell Culture by : Herbert Edward Street

Download or read book Plant Tissue and Cell Culture written by Herbert Edward Street and published by Univ of California Press. This book was released on 1973 with total page 520 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Culture of Cells for Tissue Engineering

Author : Gordana Vunjak-Novakovic
Publisher : John Wiley & Sons
ISBN 13 : 0471741809
Total Pages : 528 pages
Book Rating : 4.4/5 (717 download)

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Book Synopsis Culture of Cells for Tissue Engineering by : Gordana Vunjak-Novakovic

Download or read book Culture of Cells for Tissue Engineering written by Gordana Vunjak-Novakovic and published by John Wiley & Sons. This book was released on 2006-03-31 with total page 528 pages. Available in PDF, EPUB and Kindle. Book excerpt: Step-by-step, practical guidance for the acquisition, manipulation,and use of cell sources for tissue engineering Tissue engineering is a multidisciplinary field incorporatingthe principles of biology, chemistry, engineering, and medicine tocreate biological substitutes of native tissues for scientificresearch or clinical use. Specific applications of this technologyinclude studies of tissue development and function, investigatingdrug response, and tissue repair and replacement. This area israpidly becoming one of the most promising treatment options forpatients suffering from tissue failure. Written by leading experts in the field, Culture of Cellsfor Tissue Engineering offers step-by-step, practicalguidance for the acquisition, manipulation, and use of cell sourcesfor tissue engineering. It offers a unique focus on tissueengineering methods for cell sourcing and utilization, combiningtheoretical overviews and detailed procedures. Features of the text include: Easy-to-use format with a two-part organization Logically organized—part one discusses cell sourcing,preparation, and characterization and the second part examinesspecific engineered tissues Each chapter covers: structural and functional properties oftissues, methodological principles, culture, cellselection/expansion, cell modifications, cell seeding, tissueculture, analytical assays, and a detailed description ofrepresentative studies End-of-chapter features include useful listings of sources forreagents, materials, and supplies, with the contact details of thesuppliers listed at the end of the book A section of elegant color plates to back up the figures in thechapters Culture of Cells for Tissue Engineering givesnovice and seasoned researchers in tissue engineering an invaluableresource. In addition, the text is suitable for professionals inrelated research, particularly in those areas where cell and tissueculture is a new or emerging tool.

Plant Cell Culture

Author : Michael R. Davey
Publisher : John Wiley & Sons
ISBN 13 : 9780470686515
Total Pages : 358 pages
Book Rating : 4.6/5 (865 download)

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Book Synopsis Plant Cell Culture by : Michael R. Davey

Download or read book Plant Cell Culture written by Michael R. Davey and published by John Wiley & Sons. This book was released on 2010-05-20 with total page 358 pages. Available in PDF, EPUB and Kindle. Book excerpt: The ability to culture cells is fundamental for mass propagation and as a baseline for the genetic manipulation of plant nuclei and organelles. The introduction to Plant Cell Culture: Essential Methods provides a general background to plant cell culture, including basic principles, technologies and laboratory practices that underpin the more detailed techniques described in subsequent chapters. Whilst each chapter provides a background to the topic area and methodology, a crucial aspect is the provision of detailed protocols with emphasis on trouble shooting, describing common problems and detailed advice for their avoidance. Plant Cell Culture: Essential Methods provides the reader with a concise overview of these techniques, including micropropagation, mutagenesis, cryopreservation, genetic and plastid transformation and somatic cell technologies. This book will be an essential addition to any plant science laboratory's bookshelf. Highlights the best and most up-to-date techniques for working on plant cell culture Explains clearly and precisely how to carry out selected techniques in addition to background information on the various approaches Chapters are written by leading international authorities in the field and cover both well-known and new, tried and tested, methods for working in plant cell culture An essential laboratory manual for students and early-career researchers.

Plant Tissue Culture Manual

Author : K. Lindsey
Publisher : Springer Science & Business Media
ISBN 13 : 940090181X
Total Pages : 138 pages
Book Rating : 4.4/5 (9 download)

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Book Synopsis Plant Tissue Culture Manual by : K. Lindsey

Download or read book Plant Tissue Culture Manual written by K. Lindsey and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 138 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual comprises a range of techniques for research workers in the fields of cell and molecular biology, physiology, plant breeding and propagation, and genetic engineering.

Cell and Tissue Culture in Forestry

Author : J.M. Bonga
Publisher : Springer Science & Business Media
ISBN 13 : 9401709947
Total Pages : 436 pages
Book Rating : 4.4/5 (17 download)

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Book Synopsis Cell and Tissue Culture in Forestry by : J.M. Bonga

Download or read book Cell and Tissue Culture in Forestry written by J.M. Bonga and published by Springer Science & Business Media. This book was released on 2013-06-29 with total page 436 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the first edition of our book "Tissue Culture in Fores try" in 1982 we have witnessed remarkable advances in cell and tissue culture technologies with woody perennials. In addition to forest biologists in government, industry, and universities, we now have molecular biologists, genetic engineers, and biochemists using cell and tissue cultures of woody species routinely. There fore, the time has come for an update of the earlier edition. In our present effort to cover new developments we have expanded to three volumes: 1. General principles and Biotechnology 2. Specific Principles and Methods: Growth and Development 3. Case Histories: Gymnosperms, Angiosperms and Palms The scientific barriers to progress in tree improvement are not so much lack of foreign gene expression in plants but our current inabili ty to regenerate plants in true-to-type fashion on a mas sive and economic scale. To achieve this in the form of an appro pr iate biotechnology, cell and tissue culture will increasing ly require a better understanding of basic principles in chemistry and physics that determine structural and functional relationships among molecules and macromolecules (proteins, RNA, DNA) within cells and tissues. These principles and their relationship with the culture medium and its physical environment, principles of clonal propagation, and genetic variation and ultrastructure are discussed in volume one.

Plant Tissue Culture

Author : Robert H. Smith
Publisher : Academic Press
ISBN 13 : 0323160476
Total Pages : 190 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Plant Tissue Culture by : Robert H. Smith

Download or read book Plant Tissue Culture written by Robert H. Smith and published by Academic Press. This book was released on 2012-12-02 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: Plant Tissue Culture Techniques and Experiments is a manual that contains laboratory exercises about the demonstration of the methods and different plant materials used in plant tissue culture. It provides an overview on the plant cell culture techniques and plant material options in selecting the explant source. This book starts by discussing the proper setup of a tissue culture laboratory and the selection of the culture medium. It then explains the determination of an explant which is the ultimate goal of the cell culture project. The explant is a piece of plant tissue that is used in tissue culture. Furthermore, the book discusses topics about callus induction, regeneration and morphogenesis process, and haploid plants from anther and pollen culture. The meristem culture for virus-free plants and in vitro propagation for commercial propagation of ornamentals are also explained in this manual. The book also provides topics and exercises on the protoplast isolation and fusion and agrobacterium-mediated transformation of plants. This manual is intended for college students, both graduate and undergraduate, who study chemistry, plant anatomy, and plant physiology.

Plant Cell Culture

Author : Hamish A. Collin
Publisher : Taylor & Francis
ISBN 13 :
Total Pages : 172 pages
Book Rating : 4.:/5 (319 download)

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Book Synopsis Plant Cell Culture by : Hamish A. Collin

Download or read book Plant Cell Culture written by Hamish A. Collin and published by Taylor & Francis. This book was released on 1998 with total page 172 pages. Available in PDF, EPUB and Kindle. Book excerpt: All the information necessary to set up and run a tissue culture facility is provided in this introductory book.; ; Includes an overview of all the basic tissue culture techniques and describes in detail both the theoretical background and the practical a