Cell Biology Assays

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Cell-Based Assays for High-Throughput Screening

Author : Paul A. Clemons
Publisher : Humana Press
ISBN 13 : 9781627039079
Total Pages : 0 pages
Book Rating : 4.0/5 (39 download)

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Book Synopsis Cell-Based Assays for High-Throughput Screening by : Paul A. Clemons

Download or read book Cell-Based Assays for High-Throughput Screening written by Paul A. Clemons and published by Humana Press. This book was released on 2014-11-27 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As the use of high-throughput screening expands and creates more interest in the academic community, the need for detailed reference materials becomes ever more pressing. Cell-Based Assays for High-Throughput Screening: Methods and Protocols aims to fill an important part of this need by providing an easily accessible reference volume for cell-based phenotypic screening. Leading researchers in the field contribute state-of-the-art methods with actionable protocols covering four major areas of study: model biological systems, screening modalities and assay systems, detection technologies, and approaches to data analysis. Written in the highly successful Methods in Molecular BiologyTM series format, each chapter includes a brief introduction to the subject, lists of necessary materials and reagents, step-by-step laboratory protocols, and a Notes section detailing tips on troubleshooting and avoiding known pitfalls. Cutting-edge and easy-to-use, Cell-Based Assays for High-Throughput Screening: Methods and Protocols presents an overview of relevant approaches, enabling the direct application of existing methods to new discoveries while also inspiring researchers to approach their screening projects in a conceptually modular fashion, enhancing the power to discover through new combinations of existing approaches.

Mitochondria Biology

Author :
Publisher : Academic Press
ISBN 13 : 0128202289
Total Pages : 604 pages
Book Rating : 4.1/5 (282 download)

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Book Synopsis Mitochondria Biology by :

Download or read book Mitochondria Biology written by and published by Academic Press. This book was released on 2020-03-13 with total page 604 pages. Available in PDF, EPUB and Kindle. Book excerpt: Methods in Cell Biology Volume 155 provides an update on the step-by-step "how-to" methods to study mitochondrial structure, function and biogenesis contained in the first two editions. As in the previous editions, biochemical, cell biological, and genetic approaches are presented along with sample results, interpretations, and pitfalls for each method. New chapters in this update include Isolation of Mitochondria and Analysis of Mitochondrial Compartments, Isolation of Mitochondria from Animal Cells and Yeast, Isolation and Characterization of Mitochondria-Associated ER Membranes, Import of Proteins into Mitochondria, Proximity Labeling Methods to Assess Protein-Protein Interactions in Yeast Mitochondria, and more.

Molecular Biology of the Cell

Author :
Publisher :
ISBN 13 : 9780815332183
Total Pages : 0 pages
Book Rating : 4.3/5 (321 download)

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Book Synopsis Molecular Biology of the Cell by :

Download or read book Molecular Biology of the Cell written by and published by . This book was released on 2002 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt:

Mesenchymal Stem Cell Assays and Applications

Author : Mohan C Vemuri
Publisher : Humana Press
ISBN 13 : 9781493957798
Total Pages : 534 pages
Book Rating : 4.9/5 (577 download)

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Book Synopsis Mesenchymal Stem Cell Assays and Applications by : Mohan C Vemuri

Download or read book Mesenchymal Stem Cell Assays and Applications written by Mohan C Vemuri and published by Humana Press. This book was released on 2017-05-04 with total page 534 pages. Available in PDF, EPUB and Kindle. Book excerpt: Mesenchymal Stem Cells have seen an unprecedented level of interest in the last decade, primarily due to their relative ease of isolation, the large numbers of cells present in the adult, and the ability to propagate these cells in culture. In Mesenchymal Stem Cell Assays and Applications, expert researchers from across the globe explore the latest techniques to propagate, characterize, and engineer this special cell type. Chapters outline a set of protocols and assays used by leading investigators in the field, providing standards that can be applied by all researchers to the population of cells used in their experiments. Composed in the highly successful Methods in Molecular BiologyTM series format, each chapter contains a brief introduction, step-by-step methods, a list of necessary materials, and a Notes section which shares tips on troubleshooting and avoiding known pitfalls. Ground-breaking and current, Mesenchymal Stem Cell Assays and Applications is a necessary handbook for all researchers working with this ambiguous population of cells.

Angiogenesis Assays

Author : Carolyn A. Staton
Publisher : John Wiley & Sons
ISBN 13 : 047002934X
Total Pages : 410 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Angiogenesis Assays by : Carolyn A. Staton

Download or read book Angiogenesis Assays written by Carolyn A. Staton and published by John Wiley & Sons. This book was released on 2007-01-11 with total page 410 pages. Available in PDF, EPUB and Kindle. Book excerpt: Angiogenesis, the development of new blood vessels from the existing vasculature, is essential for physiological growth and over 18,000 research articles have been published describing the role of angiogenesis in over 70 different diseases, including cancer, diabetic retinopathy, rheumatoid arthritis and psoriasis. One of the most important technical challenges in such studies has been finding suitable methods for assessing the effects of regulators of eh angiogenic response. While increasing numbers of angiogenesis assays are being described both in vitro and in vivo, it is often still necessary to use a combination of assays to identify the cellular and molecular events in angiogenesis and the full range of effects of a given test protein. Although the endothelial cell - its migration, proliferation, differentiation and structural rearrangement - is central to the angiogenic process, it is not the only cell type involved. the supporting cells, the extracellular matrix and the circulating blood with its cellular and humoral components also contribute. In this book, experts in the use of a diverse range of assays outline key components of these and give a critical appraisal of their strengths and weaknesses. Examples include assays for the proliferation, migration and differentiation of endothelial cells in vitro, vessel outgrowth from organ cultures, assessment of endothelial and mural cell interactions, and such in vivo assays as the chick chorioallantoic membrane, zebrafish, corneal, chamber and tumour angiogenesis models. These are followed by a critical analysis of the biological end-points currently being used in clinical trials to assess the clinical efficacy of anti-angiogenic drugs, which leads into a discussion of the direction future studies should take. This valuable book is of interest to research scientists currently working on angiogenesis in both the academic community and in the biotechnology and pharmaceutical industries. Relevant disciplines include cell and molecular biology, oncology, cardiovascular research, biotechnology, pharmacology, pathology and physiology.

Mammalian Cell Viability

Author : Martin J. Stoddart
Publisher : Humana Press
ISBN 13 : 9781617791079
Total Pages : 0 pages
Book Rating : 4.7/5 (91 download)

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Book Synopsis Mammalian Cell Viability by : Martin J. Stoddart

Download or read book Mammalian Cell Viability written by Martin J. Stoddart and published by Humana Press. This book was released on 2011-04-12 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Whether the question is one of basic cell survival, or whether it is being used to correlate cell number to some other factor such as matrix synthesis, an estimate of cell viability is universally required. In Mammalian Cell Viability: Methods and Protocols, experts in the field describe methods from the most basic which can be performed in any laboratory, to some which require specific pieces of equipment. Initially focusing on methods for monolayer and suspension cells, later chapters describe methods for determining viability within tissue sections and 3 dimensional culture systems. Finally, methods requiring highly specialized equipment are described in order to explain what is possible. Written in the highly successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and vital tips on troubleshooting and avoiding known pitfalls. Practical and adaptable, Mammalian Cell Viability: Methods and Protocols serves as a self-contained laboratory manual useful to both experienced researchers and those new to this incredibly important and influential field.

Cancer Cell Culture

Author : Ian A. Cree
Publisher : Humana Press
ISBN 13 : 9781493956579
Total Pages : 502 pages
Book Rating : 4.9/5 (565 download)

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Book Synopsis Cancer Cell Culture by : Ian A. Cree

Download or read book Cancer Cell Culture written by Ian A. Cree and published by Humana Press. This book was released on 2016-08-23 with total page 502 pages. Available in PDF, EPUB and Kindle. Book excerpt: With many recent advances, cancer cell culture research is more important than ever before. This timely edition of Cancer Cell Culture: Methods and Protocols covers the basic concepts of cancer cell biology and culture while expanding upon the recent shift in cell culture methods from the generation of new cell lines to the use of primary cells. There are methods to characterize and authenticate cell lines, to isolate and develop specific types of cancer cells, and to develop new cell line models. Functional assays are provided for the evaluation of clonogenicity, cell proliferation, apoptosis, adhesion, migration, invasion, senescence, angiogenesis, and cell cycle parameters. Other methods permit the modification of cells for transfection, drug resistance, immortalization, and transfer in vivo, the co-culture of different cell types, and the detection and treatment of contamination. In this new edition, specific emphasis is placed on safe working practice for both cells and laboratory researchers. These chapters contain the information critical to success – only by good practice and quality control will the results of cancer cell culture improve. Written in the successful Methods in Molecular BiologyTM series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Cancer Cell Culture: Methods and Protocols serves as a practical guide for scientists of all backgrounds and aims to convey the appropriate sense of fascination associated with this research field.

Cellular In Vitro Testing

Author : John Haycock
Publisher : CRC Press
ISBN 13 : 9814364983
Total Pages : 186 pages
Book Rating : 4.8/5 (143 download)

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Book Synopsis Cellular In Vitro Testing by : John Haycock

Download or read book Cellular In Vitro Testing written by John Haycock and published by CRC Press. This book was released on 2014-08-27 with total page 186 pages. Available in PDF, EPUB and Kindle. Book excerpt: Growing cells in 2D under static conditions has long been the gold standard of cell culture, despite this method not being representative of the complex in vivo environment. The use of animal models also has clear ethical and scientific limitations, and increasingly the 3Rs (replacement, refinement, reduction) in relation to animal models are being

B Cell Protocols

Author : Hua Gu
Publisher : Springer Science & Business Media
ISBN 13 : 1592597963
Total Pages : 294 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis B Cell Protocols by : Hua Gu

Download or read book B Cell Protocols written by Hua Gu and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.

Current Protocols in Molecular Biology

Author :
Publisher :
ISBN 13 : 9780471503385
Total Pages : pages
Book Rating : 4.5/5 (33 download)

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Book Synopsis Current Protocols in Molecular Biology by :

Download or read book Current Protocols in Molecular Biology written by and published by . This book was released on with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:

Cell Biology Assays

Author : Fanny Jaulin
Publisher : Butterworth-Heinemann
ISBN 13 : 0123751535
Total Pages : 299 pages
Book Rating : 4.1/5 (237 download)

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Book Synopsis Cell Biology Assays by : Fanny Jaulin

Download or read book Cell Biology Assays written by Fanny Jaulin and published by Butterworth-Heinemann. This book was released on 2009-11-19 with total page 299 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text provides comprehensive protocols essential methods across cell biology. The techniques in this text are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls while enabling researchers at all stages to embark on basic problems using a variety of technologies and model systems. - Provides researchers with solutions in lab environments - Features an array of essential methods, including endocytic pathways, membranes, mitochondria, and in vitro motility - Information on a plethora of technologies needed to tackle complex problems

Laboratory Investigations in Cell and Molecular Biology

Author : Allyn Bregman
Publisher :
ISBN 13 :
Total Pages : 340 pages
Book Rating : 4.:/5 (89 download)

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Book Synopsis Laboratory Investigations in Cell and Molecular Biology by : Allyn Bregman

Download or read book Laboratory Investigations in Cell and Molecular Biology written by Allyn Bregman and published by . This book was released on 1996-02-02 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This revised workbook/lab text consists of 21 projects that can be executed with readily available materials, a minimum of elaborate equipment and a reasonable amount of preparation time. Early projects deal with biochemistry and cytochemistry; the middle ones focus on organelles and their physiology; and later activities explore more advanced molecular topics such as restriction mapping strategies. New to this edition: a concise section on statistics covering the mean, standard deviation and standard error; and a chapter designed to enable students to write up their work as a lab report.

Bioluminescence and Chemiluminescence

Author : A. Roda
Publisher : Wiley
ISBN 13 : 9780471987338
Total Pages : 648 pages
Book Rating : 4.9/5 (873 download)

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Book Synopsis Bioluminescence and Chemiluminescence by : A. Roda

Download or read book Bioluminescence and Chemiluminescence written by A. Roda and published by Wiley. This book was released on 1999-05-27 with total page 648 pages. Available in PDF, EPUB and Kindle. Book excerpt: Bioluminescence is the natural phenomenon found in light emitting organisms while chemiluminescence is a similar phenomenon in chemical reactions. This book contains the proceedings of one of the world's leading conferences in this field of study. As with previous, well-received publications of this conference, this book showcases the conference's growing importance.

Cell Biology

Author : Julio E. Celis
Publisher :
ISBN 13 : 9780121647254
Total Pages : 0 pages
Book Rating : 4.6/5 (472 download)

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Book Synopsis Cell Biology by : Julio E. Celis

Download or read book Cell Biology written by Julio E. Celis and published by . This book was released on 1998 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This Second Edition of the highly praised Cell Biology: A Laboratory Handbook brings together 260 new and revised chapters. Each chapter is concisely written and beautifully illustrated, making this attractive four-volume set a worthwhile addition to any desktop, and the up-to-date instructions for biological techniques make this reference the next best thing to having the expert at your side. Dr. Julio Celis and the Associate Editors have drawn on peer review from the scientific community to include 40 percent new material in this much-needed and updated laboratory manual. In one easy to use reference, current and classic protocols are presented in a clear and reader-friendly format that makes this manual a necessity to undergraduate and graduate students as well as technicians and instructors. Features: * Contains more than 40% new material * Provides cell biologists and other life scientists with the most up-to-date instructions for basic and advanced cell biological techniques, including those at the interface between cell and molecular biology * Features uniform style and editing and includes contributions from world-renowned authorities in their respective fields * Contains i

Molecular Cell Biology

Author : Harvey F. Lodish
Publisher : Scientific American Library
ISBN 13 :
Total Pages : 1192 pages
Book Rating : 4.:/5 (318 download)

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Book Synopsis Molecular Cell Biology by : Harvey F. Lodish

Download or read book Molecular Cell Biology written by Harvey F. Lodish and published by Scientific American Library. This book was released on 2000 with total page 1192 pages. Available in PDF, EPUB and Kindle. Book excerpt: With its acclaimed author team, cutting-edge content, emphasis on medical relevance, and coverage based on landmark experiments, "Molecular Cell Biology" has justly earned an impeccable reputation as an authoritative and exciting text. The new Sixth Edition features two new coauthors, expanded coverage of immunology and development, and new media tools for students and instructors.

T Cell Protocols

Author : Gennaro De Libero
Publisher : Methods in Molecular Biology
ISBN 13 :
Total Pages : 196 pages
Book Rating : 4.F/5 ( download)

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Book Synopsis T Cell Protocols by : Gennaro De Libero

Download or read book T Cell Protocols written by Gennaro De Libero and published by Methods in Molecular Biology. This book was released on 2009 with total page 196 pages. Available in PDF, EPUB and Kindle. Book excerpt: With a wide variety of investigative approaches, T cell immunology is a vital and open field of study. In T Cell Protocols, Second Edition, an international panel of experts contribute fully updated classic protocols as well as newly established novel techniques for the study of T lymphocyte biology. Written in the highly successful Methods in Molecular BiologyTM series format, the chapters in this volume provide brief introductions to the topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and Notes sections which collect expert tips on troubleshooting and avoiding known pitfalls. Up-to-date and easy to use, T Cell Protocols, Second Edition is an ideal guide for young investigators new to the complex field of immunology as well as a valuable, concise resource for experienced scientists searching for clear, efficacious descriptions of novel methods.

Cell Biology

Author : Julio E. Celis
Publisher : Elsevier
ISBN 13 : 0121647307
Total Pages : 632 pages
Book Rating : 4.1/5 (216 download)

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Book Synopsis Cell Biology by : Julio E. Celis

Download or read book Cell Biology written by Julio E. Celis and published by Elsevier. This book was released on 2006 with total page 632 pages. Available in PDF, EPUB and Kindle. Book excerpt: This four-volume laboratory manual contains comprehensive state-of-the-art protocols essential for research in the life sciences. Techniques are presented in a friendly step-by-step fashion, providing useful tips and potential pitfalls. The important steps and results are beautifully illustrated for further ease of use. This collection enables researchers at all stages of their careers to embark on basic biological problems using a variety of technologies and model systems. This thoroughly updated third edition contains 165 new articles in classical as well as rapidly emerging technologies. Topics covered include: Cell and Tissue Culture: Associated Techniques, Viruses, Antibodies, Immunocytochemistry (Volume 1) Organelle and Cellular Structures, Assays (Volume 2) Imaging Techniques, Electron Microscopy, Scanning Probe and Scanning Electron Microscopy, Microdissection, Tissue Arrays, Cytogenetics and In Situ Hybridization, Genomics and Transgenic Knockouts and Knock-down Methods (Volume 3) Transfer of Macromolecules, Expression Systems, Gene Expression Profiling (Volume 4) Indispensable bench companion for every life science laboratory Provides the latest information on the plethora of technologies needed to tackle complex biological problems Includes numerous illustrations, some in full color, supporting steps and results