Capillary Electrophoresis In The Life Sciences

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Capillary Gel Electrophoresis

Author : Andras Guttman
Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. - Covers all theoretical and practical aspects of capillary gel electrophoresis - Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods - Teaches readers how to use the technology and select methods that are ideal for fundamental problems - Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Capillary Electrophoresis - Mass Spectrometry (CE-MS)

Author : Gerhardus de Jong
Publisher : John Wiley & Sons
ISBN 13 : 3527339248
Total Pages : 364 pages
Book Rating : 4.5/5 (273 download)

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Book Synopsis Capillary Electrophoresis - Mass Spectrometry (CE-MS) by : Gerhardus de Jong

Download or read book Capillary Electrophoresis - Mass Spectrometry (CE-MS) written by Gerhardus de Jong and published by John Wiley & Sons. This book was released on 2016-09-13 with total page 364 pages. Available in PDF, EPUB and Kindle. Book excerpt: Diese Monographie bietet einen vollständigen Überblick über die Prinzipien und Anwendungen der Kapillarelektrophorese (CE) und der Massenspektrometrie (MS) und legt den Nachdruck insbesondere auf Kopplungsschnittstellen. Ausführlich erläutert werden auch alle relevanten Substanzklassen. Ein einzigartiges Wissenskompendium für alle, die sich CE-MS beschäftigen!

Handbook of Capillary Electrophoresis, Second Edition

Author : James P. Landers
Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)

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Book Synopsis Handbook of Capillary Electrophoresis, Second Edition by : James P. Landers

Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Capillary Electrophoresis of Nucleic Acids

Author : Keith R. Mitchelson
Publisher : Springer Science & Business Media
ISBN 13 : 1592590551
Total Pages : 481 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Microchip Capillary Electrophoresis

Author : Charles Henry
Publisher : Humana
ISBN 13 : 9781588292933
Total Pages : 238 pages
Book Rating : 4.2/5 (929 download)

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Book Synopsis Microchip Capillary Electrophoresis by : Charles Henry

Download or read book Microchip Capillary Electrophoresis written by Charles Henry and published by Humana. This book was released on 2006-03-01 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Leading chemists and engineers concisely explain the principles behind microchip capillary electrophoresis and demonstrate its use in a variety of biochemical applications, ranging from the analysis of DNA, proteins, and peptides to single cell analysis and measuring the impact of surface modification on flow in microfluidic channels. Since surface chemistry must be carefully considered for optimal operation at this scale, the authors also discuss methods of both adsorbed and covalent surface modification for its control. Fabrication methods for producing microchips with glass, poly(dimethylsiloxane), and other polymers are also provided so that even novices can produce simple devices for standard separations. Microchip Capillary Electrophoresis: Methods and Protocols provides a practical starting point for either initiating research in the field of microchip capillary electrophoresis or understanding the full range of what can be done with existing systems.

Capillary Electrophoresis

Author : Paul D. Grossman
Publisher : Academic Press
ISBN 13 : 0323138195
Total Pages : 369 pages
Book Rating : 4.3/5 (231 download)

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Book Synopsis Capillary Electrophoresis by : Paul D. Grossman

Download or read book Capillary Electrophoresis written by Paul D. Grossman and published by Academic Press. This book was released on 2012-12-02 with total page 369 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book is designed to be a practical guide, used by wide audience, including those new to CE, those more experienced, routine users, those interested in technology development, and those involved with applications research. References have been emphasized to allow the reader to explore the detailed specifics and theoretical foundations.This book draws together the rapidly evolving, diverse, and multidisciplinary subject of capillary electrophoresis (CE). It is designed as a practical guide to be used by a wide audience, including those new to CE as well as more experienced users. This volume presents the capabilities, limitations, potentials, and future challenges facing each area of CE. Key aspects of this technique, such as high resolution capability, full automation, high speed separations, quantification of nanoliter sample volumes, and simultaneous multiple detection capabilities are presented in a concise and logical fashion. This book is designed to help you make the most of your CE separations, and includes comprehensive information on:Electroosmosis, separation efficiency, and Joule heatingDetection methodsIn-depth discussion of the separation principles and capabilities of the major modes of CESieving gel electrophoresisIsoelectric focusingFree solution CEMicellar electrokinetic capillary chromatographyEntangled polymer matrix-based separationDetailed treatment of the application of CE to a wide range of molecules, supplemented with extensive "hands-on" illustrations

Capillary Electrophoresis of Carbohydrates

Author : Nicola Volpi
Publisher : Springer Science & Business Media
ISBN 13 : 1607618753
Total Pages : 286 pages
Book Rating : 4.6/5 (76 download)

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Book Synopsis Capillary Electrophoresis of Carbohydrates by : Nicola Volpi

Download or read book Capillary Electrophoresis of Carbohydrates written by Nicola Volpi and published by Springer Science & Business Media. This book was released on 2010-10-26 with total page 286 pages. Available in PDF, EPUB and Kindle. Book excerpt: Simple carbohydrates, complex oligosaccharides and polysaccharides all belong to a class of ubiquitous (macro)molecules that exhibit a wide range of biological functions, and the recent advent of enhanced enzymatic, chemical and analytical tools used to study these sugars has inaugurated a genuine explosion in the field of glycomics. Specifically, it has led to a deeper understanding of how specific sugar structures modulate cellular phenotypes, and that breakthrough has led to the discovery of new pharmaceuticals for the treatment of many serious diseases, such as cancer. The subsequent rapid expansion of this research holds high promise for future therapeutic regimens, and capillary electrophoresis (CE) refers to the range of related separation techniques that are integral to this vital research. CE uses narrow-bore fused-silica capillaries to separate a complex array of large and small molecules, and Capillary Electrophoresis of Carbohydrates offers a comprehensive look at the latest breakthroughs and improvements in CE and CE techniques applied to monosaccharides up to complex oligosaccharides and polysaccharides. It begins with an overview of the application of CE and CE- mass spectrometric in the analysis of simple carbohydrates without any previous derivatization step before discussing various detection techniques such as spectrophotometric detection, electrochemical detection and other less common techniques. It then covers in detail an array of related topics and numerous applications. It is an essential text for anyone exploring the myriad possibilities of this rapidly expanding field.

Capillary Electrophoresis

Author : Patrick Camilleri
Publisher : CRC Press
ISBN 13 : 9780849391279
Total Pages : 586 pages
Book Rating : 4.3/5 (912 download)

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Book Synopsis Capillary Electrophoresis by : Patrick Camilleri

Download or read book Capillary Electrophoresis written by Patrick Camilleri and published by CRC Press. This book was released on 1997-11-25 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.

Capillary Electrophoresis-Mass Spectrometry

Author : Christian Neusüß
Publisher : Springer Nature
ISBN 13 : 1071624938
Total Pages : 264 pages
Book Rating : 4.0/5 (716 download)

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Book Synopsis Capillary Electrophoresis-Mass Spectrometry by : Christian Neusüß

Download or read book Capillary Electrophoresis-Mass Spectrometry written by Christian Neusüß and published by Springer Nature. This book was released on 2022-08-08 with total page 264 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume details aspects and applications of interfacing capillary electrophoresis (CE) with mass spectrometry (MS). Chapters guide readers through approaches based on different types of CE-MS interfaces such as (nano)sheath liquid, porous tip, and liquid junction, as well as various capillary coatings, and a broad range of applications including several top-down and bottom-up proteomic approaches. Additionally, a list of analyte targets was provided consisting of amphetamines, antibiotics, carbohydrates (including glycosaminoglycans and glycopeptides), enantiomers, extracellular matrix metabolites, monoclonal antibodies, and nanoparticles, and therefore covers numerous fields of applications such as pharmaceutical, biomedical, food, agrochemical, and environmental analysis. Written in the format of the highly successful Methods in Molecular Biology series, each chapter includes an introduction to the topic, lists necessary materials and reagents, includes tips on troubleshooting and known pitfalls, and step-by-step, readily reproducible protocols. Authoritative and cutting-edge, Capillary Electrophoresis-Mass Spectrometry: Methods and Protocols aims to provide highly valuable information for both beginners and experts in the field be it students, technical staff, and scientists.

Laboratory Protocols in Applied Life Sciences

Author : Prakash Singh Bisen
Publisher : CRC Press
ISBN 13 : 1466553154
Total Pages : 1772 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Laboratory Protocols in Applied Life Sciences by : Prakash Singh Bisen

Download or read book Laboratory Protocols in Applied Life Sciences written by Prakash Singh Bisen and published by CRC Press. This book was released on 2014-02-26 with total page 1772 pages. Available in PDF, EPUB and Kindle. Book excerpt: As applied life science progresses, becoming fully integrated into the biological, chemical, and engineering sciences, there is a growing need for expanding life sciences research techniques. Anticipating the demands of various life science disciplines, Laboratory Protocols in Applied Life Sciences explores this development. This book covers a wide spectrum of areas in the interdisciplinary fields of life sciences, pharmacy, medical and paramedical sciences, and biotechnology. It examines the principles, concepts, and every aspect of applicable techniques in these areas. Covering elementary concepts to advanced research techniques, the text analyzes data through experimentation and explains the theory behind each exercise. It presents each experiment with an introduction to the topic, concise objectives, and a list of necessary materials and reagents, and introduces step-by-step, readily feasible laboratory protocols. Focusing on the chemical characteristics of enzymes, metabolic processes, product and raw materials, and on the basic mechanisms and analytical techniques involved in life science technological transformations, this text provides information on the biological characteristics of living cells of different origin and the development of new life forms by genetic engineering techniques. It also examines product development using biological systems, including pharmaceutical, food, and beverage industries. Laboratory Protocols in Applied Life Sciences presents a nonmathematical account of the underlying principles of a variety of experimental techniques in disciplines, including: Biotechnology Analytical biochemistry Clinical biochemistry Biophysics Molecular biology Genetic engineering Bioprocess technology Industrial processes Animal Plant Microbial biology Computational biology Biosensors Each chapter is self-contained and written in a style that helps students progress from basic to advanced techniques, and eventually design and execute their own experiments in a given field of biology.

Separation Techniques Applied to Omics Sciences

Author : Ana Valéria Colnaghi Simionato
Publisher : Springer Nature
ISBN 13 : 3030772527
Total Pages : 277 pages
Book Rating : 4.0/5 (37 download)

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Book Synopsis Separation Techniques Applied to Omics Sciences by : Ana Valéria Colnaghi Simionato

Download or read book Separation Techniques Applied to Omics Sciences written by Ana Valéria Colnaghi Simionato and published by Springer Nature. This book was released on 2021-10-09 with total page 277 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book covers liquid chromatography, gas chromatography and capillary electrophoresis, the three main separation techniques lately available, applied to key omic sciences, such as genomics, proteomics, metabolomics and foodomics. The fundamentals of each technique are not covered herein. Instead, the recent advances in such techniques are presented focusing on the application to omics analyses and unique aspects in each case. This volume intends to offer wide ranging options available to researchers on omics sciences, and how to integrate them in order to achieve the comprehension of a biological system as a whole. Omic sciences have been of ultimate importance to comprehend the complex biochemical reactions and related events that occurs upon a biological system. The classical central dogma of molecular biology, which states that genetic information flows unidirectionally from DNA to RNA and then to proteins, has been gradually replaced by the systems biology approach. This book presents a multidisciplinary approach that explains the biological system as a whole, where the entire organism is influenced by a variety of internal events as well as by the environment, showing that each level of the biological information flux may influence the previous or the subsequent one.

Capillary Electrophoresis

Author : Dale R. Baker
Publisher : Wiley-Interscience
ISBN 13 :
Total Pages : 280 pages
Book Rating : 4.3/5 (91 download)

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Book Synopsis Capillary Electrophoresis by : Dale R. Baker

Download or read book Capillary Electrophoresis written by Dale R. Baker and published by Wiley-Interscience. This book was released on 1995-03-02 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever-expanding list of sample types. In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique. The book presents clear and straightforward explanations of the method, its operating principles, and its different modes, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary gel electrophoresis, capillary isoelectric focusing, and capillary isotachophoresis. Especially helpful is the material on developing a method: it offers practical guidance on CE modes, capillaries, run buffers, voltage requirements, sample pretreatments, injection modes and amounts, temperature settings, detector selections, buffer replenishment, and data reporting. Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners. A complete and detailed index will assist the reader in quickly finding any topic of interest.

Isotachophoresis

Author : F.M. Everaerts
Publisher : Elsevier
ISBN 13 : 0080858066
Total Pages : 433 pages
Book Rating : 4.0/5 (88 download)

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Book Synopsis Isotachophoresis by : F.M. Everaerts

Download or read book Isotachophoresis written by F.M. Everaerts and published by Elsevier. This book was released on 2011-09-22 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: Isotachophoresis

Molecular Biology

Author : Burton E. Tropp
Publisher : Jones & Bartlett Publishers
ISBN 13 : 0763786632
Total Pages : 1137 pages
Book Rating : 4.7/5 (637 download)

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Book Synopsis Molecular Biology by : Burton E. Tropp

Download or read book Molecular Biology written by Burton E. Tropp and published by Jones & Bartlett Publishers. This book was released on 2012 with total page 1137 pages. Available in PDF, EPUB and Kindle. Book excerpt: Newly revised and updated, the Fourth Edition is a comprehensive guide through the basic molecular processes and genetic phenomena of both prokaryotic and eukaryotic cells. Written for the undergraduate and first year graduate students, the text has been updated with the latest data in the field. It incorporates a biochemical approach as well as a discovery approach that provides historical and experimental information within the context of the narrative.

Biological Applications of Microfluidics

Author : Frank A. Gomez
Publisher : John Wiley & Sons
ISBN 13 : 0470074833
Total Pages : 562 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Biological Applications of Microfluidics by : Frank A. Gomez

Download or read book Biological Applications of Microfluidics written by Frank A. Gomez and published by John Wiley & Sons. This book was released on 2008-02-15 with total page 562 pages. Available in PDF, EPUB and Kindle. Book excerpt: Microfluidics has numerous potential applications in biotechnology, pharmaceuticals, the life sciences, defense, public health, and agriculture. This book details recent advances in the biological applications of microfluidics, including cell sorting, DNA sequencing on-a-chip, microchip capillary electrophoresis, and synthesis on a microfluidic format. It covers microfabricated LOC technologies, advanced microfluidic tools, microfluidic culture platforms for stem cell and neuroscience research, and more. This is an all-in-one, hands-on resource for analytical chemists and researchers and an excellent text for students.

Microsystem Technology in Chemistry and Life Sciences

Author : Andreas Manz
Publisher : Springer Science & Business Media
ISBN 13 : 3540695443
Total Pages : 269 pages
Book Rating : 4.5/5 (46 download)

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Book Synopsis Microsystem Technology in Chemistry and Life Sciences by : Andreas Manz

Download or read book Microsystem Technology in Chemistry and Life Sciences written by Andreas Manz and published by Springer Science & Business Media. This book was released on 2003-09-05 with total page 269 pages. Available in PDF, EPUB and Kindle. Book excerpt: "WHAT DOES NOT NEED TO BE BIG, WILL BE SMALL", a word by an engineer at a recent conference on chips technology. This sentence is particularly true for chemistry. Microfabrication technology emerged from microelectronics into areas like mechanics and now chemistry and biology. The engineering of micron and submicron sized features on the surface of silicon, glass and polymers opens a whole new world. Micromotors smaller than human hair have been fabricated and they work fine. It is the declared goal of the authors to bring these different worlds together in this volume. Authors have been carefully chosen to guarantee for the quality of the contents. An engineer, a chemist or a biologist will find new impulses from the various chapters in this book.

Techniques in Life Science

Author : Rahul Pandey
Publisher : BFC Publications
ISBN 13 : 9390675103
Total Pages : 190 pages
Book Rating : 4.3/5 (96 download)

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Book Synopsis Techniques in Life Science by : Rahul Pandey

Download or read book Techniques in Life Science written by Rahul Pandey and published by BFC Publications. This book was released on 2021-03-01 with total page 190 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book has been written to provide an introduction to key experimental techniques from across the biosciences. The upcoming global challenges for organisms demand a lot of researches to increase our knowledge to cope up with any adverse environmental situation. The basic research in life sciences needs to understand the biological techniques properly. Considering these requirements, the book uniquely integrates the theories and practices that drive the field of molecular biology, cell biology, biochemistry, biotechnology etc. It comprehensively covers both the methods student will encounter in lab classes and those that underpin recent advances and discoveries. The older technical details like Gel-electrophoresis, Chromatography, Centrifugation, Spectroscopy etc will be helpful to grow the initial basic concepts for all type of biological researches while the modern techniques like CRISPRS, Biosensors, DNA sequencing etc will be helpful to develop skills about these upcoming technologies. Our goal is to develop the skills at degree level students in basic biological research that they will be able to plan successfully their own experiments and examine the results obtained.