Capillary Electrophoresis in Biotechnology and Environmental Analysis

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Publisher : CRC Press
ISBN 13 : 1466563699
Total Pages : 495 pages
Book Rating : 4.4/5 (665 download)

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Book Synopsis Capillary Electrophoresis in Biotechnology and Environmental Analysis by : Hasan Parvez

Download or read book Capillary Electrophoresis in Biotechnology and Environmental Analysis written by Hasan Parvez and published by CRC Press. This book was released on 2023-01-06 with total page 495 pages. Available in PDF, EPUB and Kindle. Book excerpt: This text aims to evaluate the actual impact of high-performance capillary electrophoresis on analytical biotechnology and environmental analysis. The first part of the book presents a survey of present innovations in instrument design and different methods of pre-concentration techniques in order to obtain increased separations at higher sensitivities. The second part contains articles on applications of HPCE to protein and peptide analysis. In the third part, applications of HPCE in the investigation of drug abuse and drug interactions are presented. The last two parts of the book deal with the use of HPCE at low-UV wavelengths and negative-UV absorption. The book should be of interest to those working in HPCE research and applications.

Capillary Electrophoresis in Analytical Biotechnology

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Publisher : CRC Press
ISBN 13 : 9780849378256
Total Pages : 570 pages
Book Rating : 4.3/5 (782 download)

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Book Synopsis Capillary Electrophoresis in Analytical Biotechnology by : Pier Giorgio Righetti

Download or read book Capillary Electrophoresis in Analytical Biotechnology written by Pier Giorgio Righetti and published by CRC Press. This book was released on 1995-12-18 with total page 570 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new book on capillary electrophoresis (CE) is unique in its focus on biotechnology. It is devoted to proteins, peptides, and techniques especially useful in the area of recombinant DNA products. Emphasis is also placed on glycoproteins. Because of the growing role of the glycosylation process in CE, a comprehensive chapter on the subject acts as a book within a book. Although this well-known researcher in biotechnology presents a number of chapters extensively discussing theories, important practical aspects in the routine use of capillary electrophoresis are also covered.

Capillary Electrophoresis

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Author :
Publisher : Elsevier
ISBN 13 : 9780080858609
Total Pages : 581 pages
Book Rating : 4.8/5 (586 download)

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Book Synopsis Capillary Electrophoresis by : S.F.Y. Li

Download or read book Capillary Electrophoresis written by S.F.Y. Li and published by Elsevier. This book was released on 1992-07-31 with total page 581 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electrophoresis (CE) has had a very significant impact on the field of analytical chemistry in recent years as the technique is capable of very high resolution separations, requiring only small amounts of samples and reagents. Furthermore, it can be readily adapted to automatic sample handling and real time data processing. Many new methodologies based on CE have been reported. Rapid, reproducible separations of extremely small amounts of chemicals and biochemicals, including peptides, proteins, nucleotides, DNA, enantiomers, carbohydrates, vitamins, inorganic ions, pharmaceuticals and environmental pollutants have been demonstrated. A wide range of applications have been developed in greatly diverse fields, such as chemical, biotechnological, environmental and pharmaceutical analysis. This book covers all aspects of CE, from the principles and technical aspects to the most important applications. It is intended to meet the growing need for a thorough and balanced treatment of CE. The book will serve as a comprehensive reference work. Both the experienced analyst and the newcomer will find the text useful.

Capillary Electrophoresis

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Author :
Publisher : CRC Press
ISBN 13 : 9780849391279
Total Pages : 586 pages
Book Rating : 4.3/5 (912 download)

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Book Synopsis Capillary Electrophoresis by : Patrick Camilleri

Download or read book Capillary Electrophoresis written by Patrick Camilleri and published by CRC Press. This book was released on 1997-11-25 with total page 586 pages. Available in PDF, EPUB and Kindle. Book excerpt: The importance of capillary electrophoresis (CE) as an analytical tool has increased dramatically over the last ten years. It has changed from being an exploratory technique, mainly of academic interest, to one that is applied to solve "real" analytical problems. CE is easily adapted to its various modes of operation, often requiring little more than a change of the buffer solution, and is quickly becoming the preferred technique when analyzing minute amounts of available material. Featuring new chapters on CE analysis of inorganic ions and carbohydrates, the new edition of Capillary Electrophoresis not only presents this method as an academic tool, but also provides applications for solving "real-world" analytical problems. This updated Second Edition reflects the increasing use of CE over the last 10 years, how it is being applied, and the basic theoretical aspects of the separation and detection methodology of CE. Capillary Electrophoresis: Theory and Practice will appeal to students and professionals of analytical chemistry, physical chemistry, biochemistry, and biotechnology and includes suitable experiments designed to be attempted by university or college students, or anyone else wishing to familiarize themselves with CE.

Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition

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Author :
Publisher : CRC Press
ISBN 13 : 9780849333293
Total Pages : 1592 pages
Book Rating : 4.3/5 (332 download)

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Book Synopsis Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition by : James P. Landers

Download or read book Handbook of Capillary and Microchip Electrophoresis and Associated Microtechniques, Third Edition written by James P. Landers and published by CRC Press. This book was released on 2007-12-18 with total page 1592 pages. Available in PDF, EPUB and Kindle. Book excerpt: Although capillary electrophoresis (CE) technology has evolved quickly from the research laboratory into practical application in numerous fields, many scientists still debate its merits. While the body of international CE literature continues to expand dramatically, experts still question whether it has provided the speed, resolving power, peak capacity, sensitivity, robustness, and cost-reduction promised by its pioneers. Responding to these criticisms, this third edition brings together cutting-edge researchers to demonstrate the utility of CE across a broad spectrum of disciplines including— Forensic science Medical diagnostics Pharmaceutical science Genetic analysis Biotechnology Fluid mechanics Environmental science Biomedical research Nanotechnology Proteomics Detailed Analysis of New Methodologies and Applications Eagerly awaited by researchers and technicians who transformed the first two editions into bestsellers, this latest volume once again delivers. Emphasizing microseparations and microfluidics, the Handbook of Capillary and Microchip Electrophoresis, Third Edition features new chapters describing the use of microchip electrophoresis and associated microtechniques, with a focus on the extraordinary breadth of work undertaken to expand CE methodologies in recent years. Aided by contributions from leading international experts, this text remains a seminal reference for numerous chemistry, biology, and engineering fields.

Capillary Gel Electrophoresis

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Author :
Publisher : Newnes
ISBN 13 : 0080931359
Total Pages : 391 pages
Book Rating : 4.0/5 (89 download)

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Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman

Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses

Capillary Electrophoresis of Nucleic Acids

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 1592590551
Total Pages : 481 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Nucleic Acids by : Keith R. Mitchelson

Download or read book Capillary Electrophoresis of Nucleic Acids written by Keith R. Mitchelson and published by Springer Science & Business Media. This book was released on 2008-02-05 with total page 481 pages. Available in PDF, EPUB and Kindle. Book excerpt: The development of PCR, which enables extremely small amounts of DNA to be amplified, led to the rapid development of a multiplicity of a- lytical procedures that permit use of this new resource for the analysis of genetic variation and for the detection of disease-causing mutations. The advent of capillary electrophoresis (CE), with its power to separate and a- lyze very small amounts of DNA, has also stimulated researchers to develop analytical procedures for the CE format. The advantages of CE in terms of speed and reproducibility of analyses are manifold. Furthermore, the high s- sitivity of detection, and the ability to increase sample throughput with par- lel analysis, has led to the creation of a full range of analysis of DNA molecules, from modified DNA adducts and single-strand oligonucleotides through PCR-amplified DNA fragments and whole chromosomes. Capillary Elect- phoresis of Nucleic Acids focuses on analytical protocols that can be used for detection and analysis of mutations and modification, from precise DNA loci through entire genomes of organisms. Important practical considerations for CE, such as the choice of separation media, electrophoresis conditions, and the influence of buffer additives and dyes on DNA mobility, are discussed in several key chapters and within particular applications.

Chemometric Methods in Capillary Electrophoresis

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Author :
Publisher : John Wiley & Sons
ISBN 13 : 0470530189
Total Pages : 460 pages
Book Rating : 4.4/5 (75 download)

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Book Synopsis Chemometric Methods in Capillary Electrophoresis by : Grady Hanrahan

Download or read book Chemometric Methods in Capillary Electrophoresis written by Grady Hanrahan and published by John Wiley & Sons. This book was released on 2009-10-22 with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt: Use chemometric techniques to develop optimum separation conditions for capillary electrophoreses For all its advantages, capillary electrophoresis (CE) also carries significant disadvantages for the researcher. Offering a unique blend of information from authors active in a variety of developments of chemometrics in CE, Chemometric Methods in Capillary Electrophoresis presents modern chemometric methods as an alternative to help alleviate the problems commonly encountered during routine analysis and method development. Focusing on current chemometric methods utilized in CE endeavours by research-active experts in the field, the book begins with a thorough introduction to CE and chemometric-related concepts and the need for modern chemometric methods in CE. Part 1 discusses differing types of screening designs and response surface methodology¿in an¿application based format Part 2 includes vital discussion on various exploratory data analysis, prediction, and classification techniques utilized in CE-related studies Part 3 provides practical information on modelling quantitative structure relationships Part 4 explores transformation techniques, in particular fundamental studies and applications of cross-correlation and Hadamard Transform Electrophoresis Showing how chemometric methods are applied in a wide array of applications including biological, medical, pharmaceutical, food, forensic, and environmental science, Chemometric Methods in Capillary Electrophoresis is not only highly significant to capillary electrophoresis-based endeavours, but instructive for investigators active in other areas of separation science who could benefit from its informative content.

Capillary Electrophoresis in the Life Sciences

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Publisher : Elsevier Science & Technology
ISBN 13 : 9780444828682
Total Pages : 0 pages
Book Rating : 4.8/5 (286 download)

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Book Synopsis Capillary Electrophoresis in the Life Sciences by : Ante M. Krstulović

Download or read book Capillary Electrophoresis in the Life Sciences written by Ante M. Krstulović and published by Elsevier Science & Technology. This book was released on 1997 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: High-performance capillary electrophoresis (CE) is at present widely considered as the most important milestone in the field of separation science of the last decade. The current developments in modern biology and medicine necessitate high-resolution microtechniques for the analysis of large biological molecules. Due to its high resolving power, high speed of analysis and low cost operation, CE has been extensively used in the analysis of proteins, peptides, oligonucleotides, DNA, DNA sequencing of genomic material, double-stranded DNA fragments in mutational analysis, restriction fragment mapping, etc. Its hybrids with liquid chromatography, such as micellar electrokinetic capillary chromatography and capillary electrochromatography have extended the use to relatively small organic molecules encountered in pharmaceutical, environmental, forensic and clinical analysis, as well as combinatorial chemistry and fundamental research, where its role has been complimentary to that of the well established HPLC techniques. Because of the dramatic decrease in sample volume (nanoliters and picoliters) even the analysis of single cell contents has been made possible. In order to overcome the problem of low sensitivity, on-line preconcentration techniques (isotachophoretic) sample stacking, electrochromatographic, affinity, microchips, etc.), as well as laser-induced fluorescence and various mass spectrometric techniques have been used. While the use of CE in the area of biomolecular separations is now well-established, its usefulness as an orthogonal technique with respect to HPLC in the analysis of small molecules is yet to be developed. It is hoped that the selected papers of this volume will illustrate further the tremendous potential of CE in the life sciences.

Practical Capillary Electrophoresis

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Publisher : Elsevier
ISBN 13 : 0080539343
Total Pages : 425 pages
Book Rating : 4.0/5 (85 download)

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Book Synopsis Practical Capillary Electrophoresis by : Robert Weinberger

Download or read book Practical Capillary Electrophoresis written by Robert Weinberger and published by Elsevier. This book was released on 2000-04-18 with total page 425 pages. Available in PDF, EPUB and Kindle. Book excerpt: In the 1980s, capillary electrophoresis (CE) joined high-performance liquid chromatography (HPLC) as the most powerful separation technique available to analytical chemists and biochemists. Published research using CE grew from 48 papers in the year of commercial introduction (1988) to 1200 in 1997. While only a dozen major pharmaceutical and biotech companies have reduced CE to routine practice, the applications market is showing real or potential growth in key areas, particularly in the DNA marketplace for genomic mapping and forensic identification. For drug development involving small molecules (including chiral separations), one CE instrument can replace 10 liquid chromatographs in terms of speed of analysis. CE also uses aqueous rather than organic solvents and is thus environmentally friendlier than HPLC. The second edition of Practical Capillary Electrophoresis has been extensively reorganized and rewritten to reflect modern usage in the field, with an emphasis on commercially available apparatus and reagents. This authoritative and very comprehensible treatment builds on the author's extensive experience as an instructor of short courses for the American Chemical Society and for industry. Illustrated with detailed diagrams of electrophoretic phenomena Offers step-by-step methods development schemes Presents techniques for developing quantitative, robust, and precise methods Includes an extensive troubleshooting guide Updates and greatly expands on the first edition-more than 50% of the text is new Written by an internationally recognized scientist who is an instructor for American Chemical Society short courses on HPCE

Capillary Electrophoresis of Proteins and Peptides

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 159259798X
Total Pages : 340 pages
Book Rating : 4.5/5 (925 download)

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Book Synopsis Capillary Electrophoresis of Proteins and Peptides by : Mark A. Strege

Download or read book Capillary Electrophoresis of Proteins and Peptides written by Mark A. Strege and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: Throughout the more than 20 years that have followed the beginnings of capillary electrophoresis (CE), its application to the analysis of proteins and peptides has continued to be reliable, versatile, and productive. Over time, CE has matured to become a superb complement to HLPC, and in many cases has also evolved as an automated and quantitative replacement for conventional slab gel electrophoresis methods such as SDS-PAGE and isoelectric focusing. Within Capillary Electrophoresis of Proteins and Peptides, we have assembled contributions from researchers who are applying state-of-the-art CE for protein and peptide analysis, including topics that we believe are of great potential both in the present and for the future. In comparison to traditional separation methods, CE represents a miniaturized analysis technique (especially in its microchip-based format) that is highly dependent upon the basic fundamentals of effective sample recovery and high sensitivity detection. With these issues in mind, Chapters 1–4 describe recently developed approaches for both capillary coatings and analyte detection via laser-induced fluorescence. Since the discipline of biotechnology has established itself as a primary platform for the application of CE to the analysis of proteins and peptides, Chapters 5–7 demonstrate a variety of examples of the specific techniques that have been applied for the development of biopharmaceuticals and their commercialization. The methods covered here include also the analysis of oligosaccharides from glycoproteins.

Capillary Electrophoresis in the Early Twenty-first Century

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Publisher :
ISBN 13 : 9781536152234
Total Pages : 0 pages
Book Rating : 4.1/5 (522 download)

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Book Synopsis Capillary Electrophoresis in the Early Twenty-first Century by : Dr. Oscar Núñez

Download or read book Capillary Electrophoresis in the Early Twenty-first Century written by Dr. Oscar Núñez and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) comprises a family of related separation techniques in which an electric field is used to achieve the separation of components in a mixture. One of the key features of CE is the simplicity of the instrumentation required, and today, these techniques have become powerful analytical tools for high efficiency separation, and the identification of a variety of ionic and neutral compounds. Moreover, the versatility of CE operational modes (capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary electrochromatography, capillary isoelectric focusing, isotachophoresis, non-aqueous capillary electrophoresis, etc.) and detection systems (ultraviolet, fluorescence, amperometric, mass spectrometry...) make CE useful for applications in many fields such as environmental, food, pharmaceutical, and forensic analysis. The typcial drawback of CE techniques, based on their low sensitivity when dealing with conventional UV-detection, due to their short detection pathways and the short sample volume introduced into the capillary, has been largely resolved not only by using more sensitive detectors like fluorescence, amperometric, or mass spectrometry, but by the employment of both off-line and on-line enrichment procedures. The aim of this present book is to address the state-of-the art of capillary electrophoresis techniques within this early twenty-first century, by reviewing new trends and the most relevant applications described in the literature. Uses in fields such as food, environmental, forensic and biological analysis are addressed by means of relevant application. The scope of this book is intentionally broad and is aimed at worldwide analytical laboratories, both public and private, at acadamic institutions, as well as researchers.

Capillary Electrophoresis

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Publisher : Springer Science & Business Media
ISBN 13 : 1588295397
Total Pages : 808 pages
Book Rating : 4.5/5 (882 download)

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Book Synopsis Capillary Electrophoresis by : Philippe Schmitt-Kopplin

Download or read book Capillary Electrophoresis written by Philippe Schmitt-Kopplin and published by Springer Science & Business Media. This book was released on 2008-02-25 with total page 808 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book presents a selection of current capillary electrophoresis methods used to separate representative types of molecules and particles and in combination with different detection techniques. It includes practical details which are hard to find elsewhere. The volume is intended for beginners in the field and provides an overview of the technique and a starting point for the exploration of the defined literature on different application topics.

Handbook of Capillary Electrophoresis, Second Edition

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Author :
Publisher : CRC Press
ISBN 13 : 9780849324987
Total Pages : 918 pages
Book Rating : 4.3/5 (249 download)

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Book Synopsis Handbook of Capillary Electrophoresis, Second Edition by : James P. Landers

Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.

Analytical and Preparative Separation Methods of Biomacromolecules

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Author :
Publisher : CRC Press
ISBN 13 : 1000146251
Total Pages : 482 pages
Book Rating : 4.0/5 (1 download)

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Book Synopsis Analytical and Preparative Separation Methods of Biomacromolecules by : Hassan Y. Aboul-Enein

Download or read book Analytical and Preparative Separation Methods of Biomacromolecules written by Hassan Y. Aboul-Enein and published by CRC Press. This book was released on 2020-10-15 with total page 482 pages. Available in PDF, EPUB and Kindle. Book excerpt: Reports up-to-date research developments on purifying and isolation large organic molecules. The text provides information on high-performance liquid chromatography and capillary electrophoresis (CE) as tools for analyzing biomacromolecules and developing new biochemical and medicinal compounds. It applies biochemical separation technology to the study of macromolecules such as proteins, polysaccharides, nucleic acids and more.

CE in Biotechnology: Practical Applications for Protein and Peptide Analyses

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Author :
Publisher : Springer Science & Business Media
ISBN 13 : 3322830217
Total Pages : 103 pages
Book Rating : 4.3/5 (228 download)

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Book Synopsis CE in Biotechnology: Practical Applications for Protein and Peptide Analyses by : Anthony B. Chen

Download or read book CE in Biotechnology: Practical Applications for Protein and Peptide Analyses written by Anthony B. Chen and published by Springer Science & Business Media. This book was released on 2013-04-17 with total page 103 pages. Available in PDF, EPUB and Kindle. Book excerpt: The goal of this book is to show recent developments in the CE analysis of protein pharmaceuticals. It is devoted completely to practical concerns to strengthen the use of CE within the biotechnology industry, highlighting the uses of CE in various areas of product development including formulation studies, process development, product characterization and validated lot release and stability testing.

Capillary Electrophoresis of Proteins

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Author :
Publisher : CRC Press
ISBN 13 : 1482289857
Total Pages : 301 pages
Book Rating : 4.4/5 (822 download)

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Book Synopsis Capillary Electrophoresis of Proteins by : Tim Wehr

Download or read book Capillary Electrophoresis of Proteins written by Tim Wehr and published by CRC Press. This book was released on 1998-10-20 with total page 301 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Provides practical information on the application of capillary electrophoresis (CE) to protein analysis, with an emphasis on developing and optimizing CE techniques in the laboratory. Includes separation methods bases on mass, charge, isoelectric point, molecular sieving, and affinity interactions."