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Capillary Electrophoresis And Isotachophoresis
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Author :International Symposium on Capillary Electrophoresis and Isotachophoresis (10, 1996, Praha) Publisher : ISBN 13 : Total Pages :405 pages Book Rating :4.:/5 (222 download)
Book Synopsis 10th International Symposium on Capillary Electrophoresis and Isotachophoresis by : International Symposium on Capillary Electrophoresis and Isotachophoresis (10, 1996, Praha)
Download or read book 10th International Symposium on Capillary Electrophoresis and Isotachophoresis written by International Symposium on Capillary Electrophoresis and Isotachophoresis (10, 1996, Praha) and published by . This book was released on 1997 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electromigration Separation Methods by : Colin F. Poole
Download or read book Capillary Electromigration Separation Methods written by Colin F. Poole and published by Elsevier. This book was released on 2018-04-13 with total page 628 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Electromigration Separation Methods is a thorough, encompassing reference that not only defines the concept of contemporary practice, but also demonstrates its implementation in laboratory science. Chapters are authored by recognized experts in the field, ensuring that the content reflects the latest developments in research. Thorough, comprehensive coverage makes this the ideal reference for project planning, and extensive selected referencing facilitates identification of key information. The book defines the concept of contemporary practice in capillary electromigration separation methods, also discussing its applications in small mass ions, stereoisomers, and proteins. Edited and authored by world-leading capillary electrophoresis experts Presents comprehensive coverage on the subject Includes extensive referencing that facilitates the identification of key research developments Provides more than 50 figures and tables that aid in the retention of key concepts
Author :International Symposium on Capillary Electrophoresis and Isotachophoresis Publisher : ISBN 13 : Total Pages :262 pages Book Rating :4.:/5 (257 download)
Book Synopsis The 8th International Symposium on Capillary Electrophoresis and Isotachophoresis by : International Symposium on Capillary Electrophoresis and Isotachophoresis
Download or read book The 8th International Symposium on Capillary Electrophoresis and Isotachophoresis written by International Symposium on Capillary Electrophoresis and Isotachophoresis and published by . This book was released on 1993 with total page 262 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Electrophoresis and Isotachophoresis by : Petr Bocek
Download or read book Capillary Electrophoresis and Isotachophoresis written by Petr Bocek and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Capillary Gel Electrophoresis by : Andras Guttman
Download or read book Capillary Gel Electrophoresis written by Andras Guttman and published by Newnes. This book was released on 2021-12-04 with total page 391 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary Gel Electrophoresis and Related Microseparation Techniques covers all theoretical and practical aspects of capillary gel electrophoresis. It also provides an excellent overview of the key application areas of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods. It not only gives readers a better understanding of how to utilize this technology, but also provides insights into how to determine which method will provide the best technical solutions to particular problems. This book can also serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses. Covers all theoretical and practical aspects of capillary gel electrophoresis Excellent overview of the key applications of nucleic acid, protein and complex carbohydrate analysis, affinity-based methodologies, micropreparative aspects and related microseparation methods Teaches readers how to use the technology and select methods that are ideal for fundamental problems Can serve as a textbook for undergraduate and graduate courses in analytical chemistry, analytical biochemistry, molecular biology and biotechnology courses
Book Synopsis Capillary Electrophoresis by : Dale R. Baker
Download or read book Capillary Electrophoresis written by Dale R. Baker and published by Wiley-Interscience. This book was released on 1995-03-02 with total page 280 pages. Available in PDF, EPUB and Kindle. Book excerpt: The best source of practical, easily accessible information on this exciting new technique CAPILLARY ELECTROPHORESIS Analytical chemists and biochemists have been turning to the technique of capillary electrophoresis with increasing frequency: it is fast, sensitive, easy to automate, requires only small sample volumes and reagent amounts, and has been successfully applied to an ever-expanding list of sample types. In Capillary Electrophoresis, analytical practitioners will find a complete, practical guide to the principles, forms, and instrumentation of this technique. The book presents clear and straightforward explanations of the method, its operating principles, and its different modes, including capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary gel electrophoresis, capillary isoelectric focusing, and capillary isotachophoresis. Especially helpful is the material on developing a method: it offers practical guidance on CE modes, capillaries, run buffers, voltage requirements, sample pretreatments, injection modes and amounts, temperature settings, detector selections, buffer replenishment, and data reporting. Capillary Electrophoresis will serve both as an excellent introduction to those who are new to the technique and as a comprehensive reference book to experienced practitioners. A complete and detailed index will assist the reader in quickly finding any topic of interest.
Book Synopsis Capillary Electrophoresis and Isotachophoresis by :
Download or read book Capillary Electrophoresis and Isotachophoresis written by and published by . This book was released on 1993 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Seventh International Symposium on Capillary Electrophoresis and Isotachophoresis by : International Symposium on Capillary Electrophoresis and Isotachophoresis (7, 1990, Tatranská Lomnica)
Download or read book Seventh International Symposium on Capillary Electrophoresis and Isotachophoresis written by International Symposium on Capillary Electrophoresis and Isotachophoresis (7, 1990, Tatranská Lomnica) and published by . This book was released on 1991 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis 8th International Symposium on Capillary Electrophoresis and Isotachophoresis by :
Download or read book 8th International Symposium on Capillary Electrophoresis and Isotachophoresis written by and published by . This book was released on 1992 with total page 158 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Clinical Applications of Capillary Electrophoresis by : Stephen M. Palfrey
Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 215 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Clinical Applications of Capillary Electrophoresis, Stephen Palfrey brings together for first time a collection of detailed capillary electrophoresis protocols designed exclusively for clinical applications. Written by the leading scientists who have often perfected these methods in their own laboratories, the protocols furnish new and more powerful assays for many routine serum and blood tests now regularly performed in clinical laboratories, including urine protein analysis, hemoglobin separation, and the detection of CSF proteins, lipoproteins, myoglobin, cryoglobulins, HbA1c, and cathepsin. The protocols offered for DNA studies include double-stranded DNA analysis, the prenatal diagnosis of Down's syndrome, Rh D/d genotyping, the identification of mutated p53 oncogene, and the detection of microsatellite instability in cancers. Many of the methods can be automated to replace the more costly and labor-intensive tests that are currently used in most clinical laboratories. Clinical Applications of Capillary Electrophoresis demonstrates clearly the simplicity, versatility, and power of CE over conventional methods. It offers to beginning clinical investigators, as well as established laboratories new to the technique, a representative range of highly practical CE methods-assays that are not only certain to become ever more productive, but are already eminently useful today.
Book Synopsis Drug Monitoring and Clinical Chemistry by : Georg Hempel
Download or read book Drug Monitoring and Clinical Chemistry written by Georg Hempel and published by Elsevier. This book was released on 2004-05-15 with total page 379 pages. Available in PDF, EPUB and Kindle. Book excerpt: Drug Monitoring and Clinical Chemistry, the 5th volume in the Handbook of Analytical Separations series, gives an overview about methods to analyse drugs in biological fluids. The most widely used methods to analyse drugs in biological fluids. i.e. chromatographic methods, CE and immunoassays are described in detail. For important drugs, an overview about the methods available and a comparison of the techniques should be given to enable the reader to choose the right method depending on laboratory equipment, staff, the aim of the investigation etc. Other general aspects important for conducting therapeutic drug monitoring or pharmacokinetics studies are also covered, i.e. sample preparation, validation of the analytical methods and pharmacokinetic methods for interpreting the data. Areas where therapeutic drug monitoring is used frequently such as antibiotics, immunosuppressant drugs, antipsychotic and anticancer drugs will be discussed in detail. In addition, the important field of phenotyping and genotyping for therapy optimisation with special focus on real-life applications is also covered. The book contains important information for analyst working on drug analysis in clinical chemistry, hospital pharmacists involved in therapeutic drug monitoring, other pharmacists, chemists or physicians working on pharmacokinetic studies in industry or academia. In contrast to other books in this field, this book provides up-to-date information regarding both methodology and clinical applications. For the applications, only fields are described where therapeutic drug monitoring is used in clinical routine and provides benefit to the patients. Overview of all important field where therapeutic drug monitoring is applied All relevant analytical and computational methods are discussed Written by experts with a lot of practical experience in the field
Book Synopsis Analytical Isotachophoresis by : Petr Boček
Download or read book Analytical Isotachophoresis written by Petr Boček and published by . This book was released on 1988 with total page 268 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Author :International Symposium on Capillary Electrophoresis and Isotachophoresis Publisher : ISBN 13 : Total Pages :4 pages Book Rating :4.:/5 (257 download)
Book Synopsis The 7th International Symposium on Capillary Electrophoresis and Isotachophoresis by : International Symposium on Capillary Electrophoresis and Isotachophoresis
Download or read book The 7th International Symposium on Capillary Electrophoresis and Isotachophoresis written by International Symposium on Capillary Electrophoresis and Isotachophoresis and published by . This book was released on 1991 with total page 4 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Handbook of Capillary Electrophoresis, Second Edition by : James P. Landers
Download or read book Handbook of Capillary Electrophoresis, Second Edition written by James P. Landers and published by CRC Press. This book was released on 1996-12-23 with total page 918 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because new information was discovered at an incredible rate since the publication of the successful first edition of this Handbook, this fully updated second edition covers all areas of interest in the field of capillary electrophoresis (CE). A relatively new technology, CE is a principle method for studying the physicochemical properties of proteins, peptides, and other macromolecules. Where applicable, the 30 chapters provide basic underlying theories as well as application-oriented aspects of each technique.Keep up with all the developments in this growing field with the Handbook of Capillary Electrophoresis, Second Edition - a complete guide to the fundamentals of CE and the latest research. The chapters are organized into five units: Modes: Presents a theoretical development of the basic principles governing separation with several modes, including CEC, and discusses their practical aspects. Analyte: Applies CE to the analysis of a specific class of analytes, including organic and inorganic ions, pharmaceuticals, glycoconjugates, peptides, proteins, and DNA fragments. Fundamental Aspects of CE: Technique-oriented information for the practitioner, including the importance of the sample matrix, on-line preconcentration of samples, modes of detection, and specific aspects of CE data analysis. Applications of CE: Includes single cell analysis, CE in DNA sequencing, CE as a clinical diagnostic tool, identifying and quantifying drugs, and for characterizing interacting species. Specialized Aspects of CE: Discusses interfacing CE with mass spectrometry, high-volume throughput continuous CE, microchip CE, control of EOF, and much more. The Handbook of Capillary Electrophoresis, Second Edition, pulls together diverse areas and applications of CE, resulting in an excellent tool for scientists involved in biotechnology and clinical chemistry, as well as the pharmaceutical, bioscience, chemical, and instrument-manufacturing industries. With an applications-oriented focus, the handbook is also a superb manual for workshops, seminars, and graduate courses in separation science.
Book Synopsis Instrumental and Methodological Developments for Two-dimensional Capillary-coupled Isotachophoresis/electrophoresis-mass Spectrometry by : Hannes Georg Graf
Download or read book Instrumental and Methodological Developments for Two-dimensional Capillary-coupled Isotachophoresis/electrophoresis-mass Spectrometry written by Hannes Georg Graf and published by . This book was released on 2022 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The very low sample loadability of a few nanoliters precludes the application of capillary electrophoresis-mass spectrometry (CE-MS) in environmental trace analysis. Therefore, analyte enrichment is required to achieve the desired limits of detection. This can be achieved using the inherent preconcentration abilities of isotachophoresis (ITP), when coupled online to CE-MS. In this study, a compact 2D setup for column-coupled isotachophoresis capillary electrophoresis-mass spectrometry (ITP/CE-MS) based on a modified commercial CE MS setup for the sensitive analysis of anionic analytes is presented. Beside instrumental inventions, methodological aspects are a major focus of this thesis. The selected model compounds, glyphosate and its metabolites, are inherently difficult to analyze by chromatographic methods due to their high charge and polarity, necessitating derivatization. In contrast, electrophoretic methods are ideal, because their separation mechanism requires charged analytes and allow to analyze very sample volumes The ITP/CE-MS setup was developed as a hybrid solution based on standard capillaries and a microfluidic glass chip as interface with integrated conductivity detection. This intermediate detector enabled to reliably transfer the stack of analytes between the separation dimensions. Thanks to the hybrid design, it was possible to use a single commercial CE-MS device with a modified injection system, a third vial holder (pressurizable, connectable to high voltage) and a high voltage switching box for ITP/CE-MS measurements. All modifications were flexibly installed in the commercial CE instrument and did not require any disassembly of the CE instrument. This allowed fast switching between 2D and 1D applications on the same device. To optimize the separation conditions for both dimensions, the pKa values and limiting electrophoretic mobilities for glyphosate and its metabolites were determined between pH 1.2 and 12.0 by CE coupled to conductivity detection (CE C4D) at a very high resolution using suitable buffer systems and the free AnglerFish software developed by Malý et al. (Malý et al., Electrophoresis. 2020, 41, 493-501). Thanks to the software-based correction of ionic strength effects on the analytes' effective electrophoretic mobility, the very low pKa values of the analytes were precisely determined for the first time. With these data, optimal separation conditions for CE at different pH values were simulated and confirmed in experiments. CE-MS was successfully applied to real samples. The results revealed that phosphate is the most critical matrix component. In parallel, ITP-MS was optimized to ensure preconcentration of analytes as well as an effective separation from phosphate, not included in the ITP stack. Finally, ITP-C4D/CE-MS measurements were conducted to analyze glyphosate in aqueous samples achieving impressive limits of detection in the picomolar range.
Book Synopsis Clinical Applications of Capillary Electrophoresis by : Stephen M. Palfrey
Download or read book Clinical Applications of Capillary Electrophoresis written by Stephen M. Palfrey and published by Humana. This book was released on 2013-08-17 with total page 212 pages. Available in PDF, EPUB and Kindle. Book excerpt: The term “electrophoresis” was first used by Michaelis in 1909, to - scribe the migration of colloids in an electric field. The first practical elect- phoresis method was described by Tiselius in 1937. He used a U-tube filled with buffer layered on top of sample; migration could be monitored using Schlieren optics. In zone electrophoresis, the U-tube was replaced by paper, a support material employed simply to prevent or minimize diffusion of ions, so that ions applied in a narrow strip to the paper will separate and remain as relatively discrete zones. Paper was superceded by a variety of other media, - cluding cellulose acetate, hydrolyzed starch (starch gel), agarose, and polyacry- mide. The latter, in addition to being a support medium, has size-sieving properties. From the basic zone electrophoresis, other means of separation have been dev- oped. These include, isoelectric focusing, isotachophoresis, density gradient el- trophoresis, and various forms of immunoelectrophoresis. In some ways Capillary Electrophoresis (CE) has gone full circle back to the original method of Tiselius. In its simplest form, separations occur in a buffer solution within a glass (fused silica) tube and detection occurs as sample moves past an optical window. CE has rapidly developed into a technique that rivals HPLC in its versatility. All the classical electrophoretic separations—zone, IEF, and isotachophoresis—have their counterparts in CE. Excitingly so, and - thoritatively treated in Clinical Applications of Capillary Electrophoresis.
Book Synopsis Capillary Electrophoresis in the Early Twenty-first Century by : Dr. Oscar Núñez
Download or read book Capillary Electrophoresis in the Early Twenty-first Century written by Dr. Oscar Núñez and published by . This book was released on 2019 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Capillary electrophoresis (CE) comprises a family of related separation techniques in which an electric field is used to achieve the separation of components in a mixture. One of the key features of CE is the simplicity of the instrumentation required, and today, these techniques have become powerful analytical tools for high efficiency separation, and the identification of a variety of ionic and neutral compounds. Moreover, the versatility of CE operational modes (capillary zone electrophoresis, micellar electrokinetic capillary chromatography, capillary electrochromatography, capillary isoelectric focusing, isotachophoresis, non-aqueous capillary electrophoresis, etc.) and detection systems (ultraviolet, fluorescence, amperometric, mass spectrometry...) make CE useful for applications in many fields such as environmental, food, pharmaceutical, and forensic analysis. The typcial drawback of CE techniques, based on their low sensitivity when dealing with conventional UV-detection, due to their short detection pathways and the short sample volume introduced into the capillary, has been largely resolved not only by using more sensitive detectors like fluorescence, amperometric, or mass spectrometry, but by the employment of both off-line and on-line enrichment procedures. The aim of this present book is to address the state-of-the art of capillary electrophoresis techniques within this early twenty-first century, by reviewing new trends and the most relevant applications described in the literature. Uses in fields such as food, environmental, forensic and biological analysis are addressed by means of relevant application. The scope of this book is intentionally broad and is aimed at worldwide analytical laboratories, both public and private, at acadamic institutions, as well as researchers.
