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Calcium Measurement Methods
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Book Synopsis Calcium Measurement Methods by : Alexei Verkhratsky
Download or read book Calcium Measurement Methods written by Alexei Verkhratsky and published by Humana Press. This book was released on 2012-02-25 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: Since the introduction of fluorescent calcium indicators and the subsequent development of capacities for real-time monitoring and imaging of calcium movements in the intact cells studied in isolation, in situ and in vivo, the complex and vital calcium signaling system has been illuminated, proving calcium signals to be excellent universal reporters of cellular activity. In "Calcium Measurement Methods", a team of renowned experts in the field contribute methods dedicated to calcium imaging in neural cells with a balance between the main principles of calcium imaging and specific applications of the technique to neural tissues. The book's contents show how the combination of different indicators and recently developed microscopic techniques lead to spectacular successes in the imaging of neural cells in the living brain in both physiological and pathophysiological models. As a volume in the successful NeuromethodsTM series, the chapters provide authoritative reviews including up-to-date detailed protocols of the most commonly used approaches in the field. Cutting-edge and concise, "Calcium Measurement Methods" serves as an ideal reference to aid the many scientists worldwide engaged in calcium imaging of the nervous system.
Book Synopsis A Referee Method for the Determination of Calcium in Serum by : J. Paul Cali
Download or read book A Referee Method for the Determination of Calcium in Serum written by J. Paul Cali and published by . This book was released on 1972 with total page 142 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Calcium Transport Elements in Plants by : Santosh Kumar Upadhyay
Download or read book Calcium Transport Elements in Plants written by Santosh Kumar Upadhyay and published by Academic Press. This book was released on 2021-01-08 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium Transport Elements in Plants discusses the role of calcium in plant development and stress signaling, the mechanism of Ca2+ homeostasis across plant membranes, and the evolution of Ca2+/cation antiporter (CaCA) superfamily proteins. Additional sections cover genome-wide analysis of Annexins and their roles in plants, the roles of calmodulin in abiotic stress responses, calcium transport in relation to plant nutrition/biofortification, and much more. Written by leading experts in the field, this title is an essential resource for students and researchers that need all of the information on calcium transport elements in one place. Calcium transport elements are involved in various structural, physiological and biochemical processes or signal transduction pathways in response to various abiotic and biotic stimuli. Development of high throughput sequencing technology has favored the identification and characterization of numerous gene families in plants in recent years, including the calcium transport elements. Provides a complete compilation of detailed information on Ca2+ efflux and influx transporters in plants Discusses the mode of action of calcium transport elements and their classification Explores the indispensable role of Ca2+ in numerous developmental and stress related pathways
Book Synopsis Lectins and Glycobiology by : Hans-Joachim Gabius
Download or read book Lectins and Glycobiology written by Hans-Joachim Gabius and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 524 pages. Available in PDF, EPUB and Kindle. Book excerpt: The intriguing complexity, precision, and regulation of the wide range of biologi cal processes is determined by intricate mechanisms of molecular recognition. Their nature is under intense scrutiny. In addition to the well-appreciated interac tion of proteins either with amino acid or nucleotide sequences, the investigation of their interplay with carbohydrate elements of cellular glycoconjugates current ly exerts increasing attraction. In the group of carbohydrate-binding proteins, lec tins are distinguished from antibodies or ligand-affecting enzymes, according to the most recent definition. The thorough analysis of their structure and function is considered as a focus to collect a critical mass of information for delineating details of a further array of biochemical processes with pivotal physiological im pact. Following an already century-long history of scientific description, reflected by subjectively chosen highlights (see the Brief History of Lectin Research at page VI), the excitement in glycobiological research that prevails today can easily be ex plained by our growing awareness of the multifarious significance of a sugar-code system of biological information. This present notion unmistakably has an im pact on lines of research in diverse disciplines like cell and molecular biology, histochemistry, or clinical sciences. It also prompts inherent practical questions such as how to obtain lectins, or how to employ them as instruments in various assay systems with the best possible results. Thus, this book is devoted intentionally to cover the techniques in different research fields that deal with lectins.
Book Synopsis Evaluation of a Rapid Micro Method for the Determination of Serum Calcium by : Howard C. Leifheit
Download or read book Evaluation of a Rapid Micro Method for the Determination of Serum Calcium written by Howard C. Leifheit and published by . This book was released on 1952 with total page 42 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Calcium-regulating Hormones by : Hirotoshi Morii
Download or read book Calcium-regulating Hormones written by Hirotoshi Morii and published by . This book was released on 1991 with total page 168 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Methods for Measuring the Fractions of Calcium in Human Serum by : John G. Toffaletti
Download or read book Methods for Measuring the Fractions of Calcium in Human Serum written by John G. Toffaletti and published by . This book was released on 1977 with total page 162 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Calcium Entry Channels in Non-Excitable Cells by : Juliusz Ashot Kozak
Download or read book Calcium Entry Channels in Non-Excitable Cells written by Juliusz Ashot Kozak and published by CRC Press. This book was released on 2017-07-14 with total page 343 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium Entry Channels in Non-Excitable Cells focuses on methods of investigating the structure and function of non-voltage gated calcium channels. Each chapter presents important discoveries in calcium entry pathways, specifically dealing with the molecular identification of store-operated calcium channels which were reviewed by earlier volumes in the Methods in Signal Transduction series. Crystallographic and pharmacological approaches to the study of calcium channels of epithelial cells are also discussed. Calcium ion is a messenger in most cell types. Whereas voltage gated calcium channels have been studied extensively, the non-voltage gated calcium entry channel genes have only been identified relatively recently. The book will fill this important niche.
Book Synopsis Measuring Calcium and Calmodulin Inside and Outside Cells by : Ole H. Petersen
Download or read book Measuring Calcium and Calmodulin Inside and Outside Cells written by Ole H. Petersen and published by Springer Science & Business Media. This book was released on 2001-03-13 with total page 320 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of signal transduction mechanisms has become one of the most important branches of biomedical science, indispensable to understanding the normal actions of hormones, neurotransmitters and other extracellular signaling molecules as well as many pathophysiological processes, including inflammation and cancer. In this volume, the various techniques for measuring Ca2+ in the cytosol, in the various organelles, and in the immediate surroundings of individual cells are described in practical detail by experts. Also included is a chapter on tracking calmodulin inside cells using fluorescence technology. A Springer Lab Manual
Book Synopsis A Referee Method for the Determination of Calcium in Serum by : J. Paul Cali
Download or read book A Referee Method for the Determination of Calcium in Serum written by J. Paul Cali and published by . This book was released on 1972 with total page 144 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Proposed Reference Method for the Measurement of Ionized Calcium in Blood by : Patricia MacGilvray Kelly
Download or read book Proposed Reference Method for the Measurement of Ionized Calcium in Blood written by Patricia MacGilvray Kelly and published by . This book was released on 1993 with total page 182 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Practical Guide to the Study of Calcium in Living Cells by : Richard Nuccitelli
Download or read book A Practical Guide to the Study of Calcium in Living Cells written by Richard Nuccitelli and published by Academic Press. This book was released on 1994-03-10 with total page 418 pages. Available in PDF, EPUB and Kindle. Book excerpt: A Practical Guide to the Study of Calcium in Living Cells describes popular techniques along with helpful do's and don't's and computer programs. The volume enables investigators to evaluate confocal images, use the latest dyes, and design Calcium buffers appropriate to their research needs. This book is designed for laboratory use by graduate students, technicians, and researchers in many disciplines, ranging from molecular to cellular levels of investigation. Describes techniques for detection of [Ca2+]I: Ca2+ - sensitive microelectrodes Fluorescent dyes Luminescent proteins Includes techniques for perturbing intracellular Ca2+ Covers detailed methodology plus problems and pitfalls of each technique Contains a practical guide to preparing Ca2+ buffers with an easy-to-use computer program Color plates illustrate techniques such as Confocal ratio-imaging Use of aequorin
Download or read book Calcium in Living Cells written by and published by Academic Press. This book was released on 2010-11-26 with total page 337 pages. Available in PDF, EPUB and Kindle. Book excerpt: Every cell of the body is dependent on calcium to function. Calcium is found in teeth and bones, and calcium signalling is necessary for the movement of muscles and for the action of the heart and the intestines as well as blood coagulation. Calcium in Living Cells will update classic techniques in detecting microscopic levels of calcium ions (Ca2+) in living cells, as well as address new techniques in the field of calcium detection and calcium signaling. Such detection and measurement of intracellular calcium is important to researchers studying the heart, musculoskeletal, gastrointestinal, and immune systems, whose findings will aid in the advancement of drug and genomic therapies to treat heart, gastrointestinal, autoimmune, and infectious diseases. Gives researchers much needed information on how to study calcium in live cells, which is becoming increasingly important in heart, musculoskeletal, and immune system research Provides an overview of the latest methods--fluorescence resonance energy transfer (FRET), for example-- that are new to the field Allows understanding of how calcium plays a role in intracellular function at the cellular level, which has proved important in Alzheimer's research, heart disease, and areas of musculoskeletal research Updated chapters reflect advancements in the classic techniques used'preparing calcium buffers, vibrating the Ca2+ Electrode and confocal imaging
Book Synopsis Measurement of Calcium Ion Concentration Using Ratiometry and a Technique for Stereoscopic Imaging by :
Download or read book Measurement of Calcium Ion Concentration Using Ratiometry and a Technique for Stereoscopic Imaging written by and published by . This book was released on 2005 with total page 108 pages. Available in PDF, EPUB and Kindle. Book excerpt: Calcium acts as a universal 2nd messenger and cannot be visualized directly. Specific molecules are used that have optical properties which change upon interacting with calcium. Calcium concentrations can change in milli-seconds and regulates cellular processes such as muscle contraction, fertilization, cell division, blood clotting, and synaptic transmission. Using fluorescence, one can monitor very rapid changes in ion concentration. Changes in fluorescence intensity on the order of pico seconds can be also detected. Because it is a non-invasive technique, fluorescence does not interfere with a sample. The ratio fluorescence technique was developed to identify the location of intracellular free calcium in living cells, to measure the concentration of intracellular ions, and to monitor the changes in intracellular ions. Until the introduction of such dyes, it was not possible to detect calcium, sodium, potassium and magnesium ions by means of fluorescence because these substances do not naturally fluoresce. When they bind to an ion, their fluorescent properties are changed, and by quantitatively interpreting these changes, the concentration of the ion being investigated can be measured. This paper discusses the Ratiometric method used to measure calcium ion concentration using Fluorescent Dyes. (Abstract shortened by UMI.).
Book Synopsis Measurement of Calcium Activity in Oral Fluidsby Ion Selective Electrode: Method Evaluation and Simplified Calculation of Ion Activity Products by :
Download or read book Measurement of Calcium Activity in Oral Fluidsby Ion Selective Electrode: Method Evaluation and Simplified Calculation of Ion Activity Products written by and published by DIANE Publishing. This book was released on with total page 8 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Calcium Signaling Protocols by : David G. Lambert
Download or read book Calcium Signaling Protocols written by David G. Lambert and published by Springer Science & Business Media. This book was released on 2008-02-03 with total page 351 pages. Available in PDF, EPUB and Kindle. Book excerpt: 2+ The regulation of intracellular Ca is a common theme presented in many 2+ papers over the last 20 or so years, and the description of the Ca -sensitive indicator dye fura 2 in 1985 resulted in a massive increase in these types of 2+ studies. Aspects of the regulation of intracellular Ca have been dealt with in many of the subsequent chapters and will therefore not be covered again. Calcium Signaling Protocols results from a chance discussion with Dr. R. I. Norman of the Department of Medicine at Leicester University and r- resents a major effort from a group of extremely helpful and very patient - thors. Putting a book like this together takes time and I am indebted to these authors without whom this project would have remained a chance discussion. I am also very grateful to Professor J. M. Walker, the series editor, for all his help and advice over the course of this project and particularly his help editing the first batch of chapters. I would also like to thank Dr E. L. Pallett for help and advice regarding interconversion of Mac and Word files and for archiving chapters.
Book Synopsis Calcium Signaling by : Caroline M. Gorvin
Download or read book Calcium Signaling written by Caroline M. Gorvin and published by Humana. This book was released on 2024-11-06 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This detailed volume explores techniques for performing calcium signaling in diverse cell types, alongside detailed methods for data analysis. Beginning with chapters on cellular assays to assess calcium signaling, the book continues with sections on imaging methods, methods to assess calcium within primary cilia, the endoplasmic reticulum, and the mitochondria, as well as tissue-specific calcium signaling methods, including the establishment of primary cell or tissue models and measuring calcium in whole organisms such as Drosophila. Written for the highly successful Methods in Molecular Biology series, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step and readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Calcium Signaling: Methods and Protocols serves as an ideal guide for laboratories of all types seeking to measure calcium robustly.
