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Bone Research Protocols Methods In Molecular Medicine
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Book Synopsis Bone Research Protocols by : Miep H. Helfrich
Download or read book Bone Research Protocols written by Miep H. Helfrich and published by Springer Science & Business Media. This book was released on 2008-02-01 with total page 444 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).
Book Synopsis Bone Research Protocols. Methods in Molecular Medicine by : Miep H. Helfrich
Download or read book Bone Research Protocols. Methods in Molecular Medicine written by Miep H. Helfrich and published by . This book was released on 2003 with total page pages. Available in PDF, EPUB and Kindle. Book excerpt: The past decade has witnessed the discovery of many genes with profound effects on bone. In Bone Research Protocols, recognized authorities with extensive hands-on experience present a wide-ranging catalog of laboratory techniques that will help researchers to investigate the mechanisms by which these genes affect bone mass and strength. Described in step-by-step detail, these readily reproducible methods cover such topics as the isolation and culture of bone cells, the preparation of bone tissue for histological and ultrastructural analysis, methods for the measurement of bone strength and for mechanical studies, and how to use digital imaging techniques in the analysis of bone. Though in vitro methods receive central attention, there are also protocols for in vivo analyses of bone formation and resorption. State-of-the-art and highly practical, Bone Research Protocols offers both beginning and advanced laboratory researchers a major compendium of well-established techniques for studying bone cell function and metabolism.
Book Synopsis Bone Research Protocols by : Miep H. Helfrich
Download or read book Bone Research Protocols written by Miep H. Helfrich and published by Humana. This book was released on 2010-11-09 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The last decade has seen a tremendous advance in our understanding of bone biology. The genes responsible for the majority of rare inherited bone disorders have been identified and much progress has been made in the identification of genes in polygenic disorders such as Paget’s disease and complex multigene diseases such as osteoporosis. Transgenic technology has identified further genes, sometimes unexpectedly, with profound effects on bone. This wealth of new genetic information will undoubtedly lead to extensive cell biological studies to understand the mechanisms by which these gene products affect bone mass and bone strength. In Bone Research Protocols a catalogue of protocols has been assembled to perform such mechanistic studies. In the tradition of the Methods in Molecular Medicine series, the chapters are practical laboratory protocols that should enable the reader to carry out the techniques from scratch. To our knowledge this is the first time such a truly practical manual on well-established bone methods has been assembled, and this volume aims to be complementary to and follow on from the more theoretical Methods in Bone Biology, edited by Arnett and Henderson (1).
Book Synopsis Osteoporosis by : Jennifer J. Westendorf
Download or read book Osteoporosis written by Jennifer J. Westendorf and published by Humana Press. This book was released on 2014-11-03 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. This is the perfect guide for both new and experienced experimentalists attempting to study osteoporosis.
Book Synopsis Osteoporosis by : Jennifer J. Westendorf
Download or read book Osteoporosis written by Jennifer J. Westendorf and published by Humana Press. This book was released on 2008-04-16 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. This is the perfect guide for both new and experienced experimentalists attempting to study osteoporosis.
Book Synopsis Bone Morphogenetic Proteins by : Melissa B. Rogers
Download or read book Bone Morphogenetic Proteins written by Melissa B. Rogers and published by Humana. This book was released on 2018-11-10 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume provides methods to study bone morphogenetic proteins (BMPs) and BMP modulators in cell culture, in both invertebrate and vertebrate animal models, and for therapeutic applications. Chapters guide the reader in the use of primary and immortalized cell lines, methodologies that exploit transcription and receptor mechanisms, newly developed animal models using nematodes, flies, amphioxus, frogs, zebrafish, and genetically engineered mice, and pre-clinical approaches to understanding BMP function in bone regeneration, heterotopic ossification, and cancers. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bone Morphogenetic Proteins: Methods and Protocols aims to ensure successful results in the further study of this vital field.
Book Synopsis Bone Research Protocols by : Aymen I. Idris
Download or read book Bone Research Protocols written by Aymen I. Idris and published by . This book was released on 2019 with total page 670 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Skeletal Development and Repair by : Matthew J. Hilton
Download or read book Skeletal Development and Repair written by Matthew J. Hilton and published by Humana. This book was released on 2020-11-17 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This second edition provides a comprehensive laboratory manual on skeletal development and skeletal repair research utilizing mouse models. Chapters provide methods and protocols on the most current and cutting-edge techniques in skeletal development and repair, histological, cellular, and molecular techniques. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Skeletal Development and Repair: Methods and Protocols-Second Edition aims to ensure successful results in the further study of this vital field.
Book Synopsis Osteoporosis by : Jennifer J. Westendorf
Download or read book Osteoporosis written by Jennifer J. Westendorf and published by Humana Press. This book was released on 2008-04-16 with total page 354 pages. Available in PDF, EPUB and Kindle. Book excerpt: The study of bone cells and tissues at the cellular and molecular levels in a variety of models has revolutionized the field. In this book, leading scientists from around the world share their step-by-step laboratory protocols for studying bone biology. This is the perfect guide for both new and experienced experimentalists attempting to study osteoporosis.
Book Synopsis Bone Marrow Environment by : Marion Espéli
Download or read book Bone Marrow Environment written by Marion Espéli and published by Humana. This book was released on 2022-06-15 with total page 340 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume brings together classical and cutting-edge protocols on the spatio-temporal study of the cellular subsets constituting the bone and the marrow in both mouse and human. Chapters details methods on bone marrow (BM) ecosystem, to label, sort, analyse, and culture specific cell subsets as well as techniques allowing the evaluation of the function of some of the cellular elements of the BM. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and tips on troubleshooting and avoiding known pitfalls. Authoritative and cutting-edge, Bone Marrow Environment: Methods and Protocols aims to help new investigators to pursue the characterization of the BM microenvironment in the coming years.
Book Synopsis Osteoporosis and Osteoarthritis by : Jennifer J. Westendorf
Download or read book Osteoporosis and Osteoarthritis written by Jennifer J. Westendorf and published by Humana Press. This book was released on 2014-10-21 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: In Osteoporosis and Osteoarthritis expert investigators detail modern tools to enhance discovery in bone and cartilage biology and promote translational research in osteoporosis and osteoarthritis. New chapters describe methods to generate induced pluripotent stem cells, perform high-throughput chromatin analysis, assess loads on bone cells in vivo and in vitro, use state-of-the art imaging techniques to measure articular cartilage thickness and biochemical properties, and establish animal models of osteoarthritis and bone healing. Written in the highly successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible laboratory protocols, and key tips on troubleshooting and avoiding known pitfalls. Authoritative and practical, Osteoporosis and Osteoarthritis shares successful techniques that can be applied to the study of either disease.
Book Synopsis Basic Cell Culture Protocols by : Jeffrey W. Pollard
Download or read book Basic Cell Culture Protocols written by Jeffrey W. Pollard and published by Springer Science & Business Media. This book was released on 1997 with total page 477 pages. Available in PDF, EPUB and Kindle. Book excerpt: Now completely revised and updated from the original, much-acclaimed and bestselling first edition, Basic Cell Culture Protocols, 2nd ed. offers today's most comprehensive collection of easy-to-follow, cutting-edge protocols for the culture of a wide range of animal cells. Its authoritative contributors provide explicit, step-by-step instructions, along with extensive notes and tips that allow both experts and beginners to successfully achieve their desired results. Topics range from basic culture methodology to strategies for culturing previously uncultured cell types and hard-to-culture differentiated cells. Methods are also provided for the analysis of living cells by FACS, video microscopy, and confocal microscopy. Like the first edition, this book should be in every cell culture laboratory and be of use to all who use cell cultures in research.
Download or read book B Cell Protocols written by Hua Gu and published by Springer Science & Business Media. This book was released on 2008-02-04 with total page 294 pages. Available in PDF, EPUB and Kindle. Book excerpt: B-lymphocyte development and function remains an exciting area of research for those interested in the physiology and pathology of the immune system in higher animals. While recent advances in genetics and cellular and molecular biology have provided a large spectrum of powerful new experimental tools in this field, it is both time consuming and often very difficult for a student or just any bench-side worker to identify a reliable experimental protocol in the ocean of the literature. The aim of B Cell Protocols is to provide a collection of diverse protocols ranging from the latest inventions and applications to some classic, but still frequently used methods in B-cell biology. The authors of the various chapters are all highly qualified scientists who are either the inventors or expert users of these methods. Their extensive experience in mastering a particular method provides not only the step-by-step details of a reproducible protocol, but also useful troubleshooting tips that readers will appreciate in their daily work. We hope that this book will be helpful for both beginning and experienced researchers in the field in designing or modifying an experimental approach, and exploring a biological question from multiple angles.
Book Synopsis Stem Cell Protocols by : Ivan N. Rich
Download or read book Stem Cell Protocols written by Ivan N. Rich and published by Humana Press. This book was released on 2014-11-11 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume presents up-to-date methods that allow primary stem cells from a variety of sources to be isolated, cultured in vitro, detected and measured for specific applications. These applications range from those in basic, stem cell and veterinary research to toxicology, cellular therapy and regenerative medicine. There is a slight bias towards the blood-forming system as more is known about the blood-forming or hematopoietic system than any other primary stem cell system. These unique properties and characteristics are discussed and examined, mostly at the cellular level and in detail in this book. Written in the successful Methods in Molecular Biology series format, chapters include introductions to their respective topics, lists of the necessary materials and reagents, step-by-step, readily reproducible protocols, and notes on troubleshooting and avoiding known pitfalls. Authoritative and accessible, Stem Cell Protocols provides novices with the fundamentals necessary to develop new technologies necessary for basic and clinical research in the future, and will aid professionals in finding new methodologies to provide a wider viewpoint and an even greater scope for their own research.
Book Synopsis Flow Cytometry Protocols by : Teresa S. Hawley
Download or read book Flow Cytometry Protocols written by Teresa S. Hawley and published by Springer Nature. This book was released on with total page 460 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis A Practical Manual for Musculoskeletal Research by : Kwok-Sui Leung
Download or read book A Practical Manual for Musculoskeletal Research written by Kwok-Sui Leung and published by World Scientific. This book was released on 2008 with total page 941 pages. Available in PDF, EPUB and Kindle. Book excerpt: This manual provides technical protocols for musculoskeletal research on a translational basis, i.e. a disease-orientated approach. It offers guidance on various laboratory techniques, including cell culture and molecular biology, histology and histomorphometry, microscopy and bioimaging, laboratory animal models, CT- and MRI-based densitometry and microarchitectural analysis, biomechanics and functional analysis of orthopedic kinesiology, etc. The content is simple and straightforward, with illustrations and step-by-step procedures as an easy experimental reference for personnel in basic and clinical musculoskeletal research and education. This book will provide a unique multidisciplinary platform for various professions OCo not only orthopedics, but also biomedical engineering and biomaterial sciences OCo involving both basic and clinical medicine."
Book Synopsis Mammary Stem Cells by : Maria del Mar Vivanco
Download or read book Mammary Stem Cells written by Maria del Mar Vivanco and published by Humana. This book was released on 2015-06-04 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: The identification of normal and breast cancer stem cells has offered a new vision of this heterogeneous disease and new hopes for its prognosis and treatment. This volume provides an overview of recent developments in mammary stem cell research and discusses the many varieties of approaches used by researchers to investigate the properties and functions of mammary stem cells. The beginning chapters provide readers with an introduction to mammary stem cells, and the processes used to characterize stem cells and isolate them via fluorescent activated cell sorting. The next few chapters discuss DNA and mRNA sequencing, proteomic techniques to help profile cells, lentiviral cell transduction for gene expression, and in vivo lineage tracing. The final few chapters are dedicated to following stem cells from their initial niche to the new microenvironment at their metastasis site, and to studying these cells using physical and mathematical approaches. Written in the highly successful Methods in Molecular Biology series format, the chapters include the kind of detailed description and implementation advice that is crucial for getting optimal results in the laboratory. Authoritative and cutting-edge, Mammary Stem Cells: Methods and Protocols aims to help members of the scientific community explore the behavior of stem cells and how to work with them in order to guide the design of new and complimentary strategies to be applied in the clinic with the ultimate end goal of fighting breast cancer.
