Biological Applications Of The Atomic Force Microscope Microform Measuring The Forces That Hold Dna Together And Molecular Level Direct Real Time Observation Of The Interactions Of Reca Protein With Double Stranded Dna

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Biological Applications of the Atomic Force Microscope [microform] : Measuring the Forces that Hold DNA Together, And, Molecular Level, Direct, Real Time Observation of the Interactions of RecA Protein with Double Stranded DNA

Author : Bernard David Sattin
Publisher : Library and Archives Canada = Bibliothèque et Archives Canada
ISBN 13 : 9780612942622
Total Pages : 452 pages
Book Rating : 4.9/5 (426 download)

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Book Synopsis Biological Applications of the Atomic Force Microscope [microform] : Measuring the Forces that Hold DNA Together, And, Molecular Level, Direct, Real Time Observation of the Interactions of RecA Protein with Double Stranded DNA by : Bernard David Sattin

Download or read book Biological Applications of the Atomic Force Microscope [microform] : Measuring the Forces that Hold DNA Together, And, Molecular Level, Direct, Real Time Observation of the Interactions of RecA Protein with Double Stranded DNA written by Bernard David Sattin and published by Library and Archives Canada = Bibliothèque et Archives Canada. This book was released on 2004 with total page 452 pages. Available in PDF, EPUB and Kindle. Book excerpt: Employing the Atomic Force Microscope (AFM), two biological systems are examined under biological conditions. The force holding together single base pairs of DNA is probed. Measuring the force required to rupture a perfect double helix, a double helix with a single mismatch, and a double helix with a double mismatch, arranged in a microarray, the need for precise determination of the AFM tip spring constant is precluded. The large volume of data collected required the implementation of cluster analysis, used for the first time in single molecule force spectroscopy. The rupture force of the double helix ranged from 10 to 80 pN depending on the length of the double helix. Importantly, we found that the force contribution from both AT and GC base pairs is the same. RecA protein's interaction with double stranded DNA is studied using time resolved AFM. The RecA/DNA nucleofilament is formed under physiological conditions and imaged. Several different species are formed, and have a pitch of 10 nm. The assembly of the RecA/DNA complex is monitored by examining contour length change over time. The assembly pathway occurred via a slow nucleation step, followed by a rapid growth phase. The lag time was 25 minutes, and the growth time was 17 minutes. The maximum growth speed was 92 RecA per minute. The disassembly of the complex is monitored in real time under buffer at pH 7.4 and 5.6, at a time resolution on the order of 2 minutes, and a spatial resolution better then 50 nm. The complexes disassemble from multiple discrete nodes in a non-random manner. At pH 7.4 the disassembly is rapid (up to 39 monomers per minute per node), and two to four times slower (as slow as 4.5 monomers per minute per node) at pH 5.6. The design and implementation of a hot stage is described. The hot stage employs a simple, cost-effective design that allows a user to easily heat an AFM sample to 37 Celsius for experiments on biological systems. This is demonstrated by imaging DNA at ambient and physiological temperature.

Atomic Force Microscopy in Liquid

Author : Arturo M. Baró
Publisher : John Wiley & Sons
ISBN 13 : 3527649824
Total Pages : 385 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Atomic Force Microscopy in Liquid by : Arturo M. Baró

Download or read book Atomic Force Microscopy in Liquid written by Arturo M. Baró and published by John Wiley & Sons. This book was released on 2012-08-01 with total page 385 pages. Available in PDF, EPUB and Kindle. Book excerpt: About 40 % of current atomic force microscopy (AFM) research is performed in liquids, making liquid-based AFM a rapidly growing and important tool for the study of biological materials. This book focuses on the underlying principles and experimental aspects of AFM under liquid, with an easy-to-follow organization intended for new AFM scientists. The book also serves as an up-to-date review of new AFM techniques developed especially for biological samples. Aimed at physicists, materials scientists, biologists, analytical chemists, and medicinal chemists. An ideal reference book for libraries. From the contents: Part I: General Atomic Force Microscopy * AFM: Basic Concepts * Carbon Nanotube Tips in Atomic Force Microscopy with * Applications to Imaging in Liquid * Force Spectroscopy * Atomic Force Microscopy in Liquid * Fundamentals of AFM Cantilever Dynamics in Liquid * Environments * Single-Molecule Force Spectroscopy * High-Speed AFM for Observing Dynamic Processes in Liquid * Integration of AFM with Optical Microscopy Techniques Part II: Biological Applications * DNA and Protein-DNA Complexes * Single-Molecule Force Microscopy of Cellular Sensors * AFM-Based Single-Cell Force Spectroscopy * Nano-Surgical Manipulation of Living Cells with the AFM

Atomic Force Microscopy For Biologists (2nd Edition)

Author : Victor J Morris
Publisher : World Scientific
ISBN 13 : 190897821X
Total Pages : 423 pages
Book Rating : 4.9/5 (89 download)

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Book Synopsis Atomic Force Microscopy For Biologists (2nd Edition) by : Victor J Morris

Download or read book Atomic Force Microscopy For Biologists (2nd Edition) written by Victor J Morris and published by World Scientific. This book was released on 2009-08-11 with total page 423 pages. Available in PDF, EPUB and Kindle. Book excerpt: Atomic force microscopy (AFM) is part of a range of emerging microscopic methods for biologists which offer the magnification range of both the light and electron microscope, but allow imaging under the 'natural' conditions usually associated with the light microscope. To biologists, AFM offers the prospect of high resolution images of biological material, images of molecules and their interactions even under physiological conditions, and the study of molecular processes in living systems. This book provides a realistic appreciation of the advantages and limitations of the technique and the present and future potential for improving the understanding of biological systems.The second edition of this bestseller has been updated to describe the latest developments in this exciting field, including a brand new chapter on force spectroscopy. The dramatic developments of AFM over the past ten years from a simple imaging tool to the multi-faceted, nano-manipulating technique that it is today are conveyed in a lively and informative narrative, which provides essential reading for students and experienced researchers alike./a

High-Speed Atomic Force Microscopy in Biology

Author : Toshio Ando
Publisher : Springer Nature
ISBN 13 : 3662647850
Total Pages : 327 pages
Book Rating : 4.6/5 (626 download)

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Book Synopsis High-Speed Atomic Force Microscopy in Biology by : Toshio Ando

Download or read book High-Speed Atomic Force Microscopy in Biology written by Toshio Ando and published by Springer Nature. This book was released on 2022-03-23 with total page 327 pages. Available in PDF, EPUB and Kindle. Book excerpt: This first book on high-speed atomic force microscopy (HS-AFM) is intended for students and biologists who want to use HS-AFM in their research. It provides straightforward explanations of the principle and techniques of AFM and HS-AFM. Numerous examples of HS-AFM studies on proteins demonstrate how to apply this new form of microscopy to specific biological problems. Several precautions for successful imaging and the preparation of cantilever tips and substrate surfaces will greatly benefit first-time users of HS-AFM. In turn, the instrumentation techniques detailed in Chapter 4 can be skipped, but will be useful for engineers and scientists who want to develop the next generation of high-speed scanning probe microscopes for biology. The book is intended to facilitate the first-time use of this new technique, and to inspire students and researchers to tackle their own specific biological problems by directly observing dynamic events occurring in the nanoscopic world. Microscopy in biology has recently entered a new era with the advent of high-speed atomic force microscopy (HS-AFM). Unlike optical microscopy, electron microscopy, and conventional slow AFM, it allows us to directly observe biological molecules in physiological environments. Molecular “movies” created using HS-AFM can directly reveal how molecules behave and operate, without the need for subsequent complex analyses and roundabout interpretations. It also allows us to directly monitor morphological change in live cells, and dynamic molecular events occurring on the surfaces of living bacteria and intracellular organelles. As HS-AFM instruments were recently commercialized, in the near future HS-AFM is expected to become a common tool in biology, and will enhance and accelerate our understanding of biological phenomena.

Force Microscopy

Author : Bhanu P. Jena
Publisher : Wiley-Liss
ISBN 13 : 0470007699
Total Pages : 433 pages
Book Rating : 4.4/5 (7 download)

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Book Synopsis Force Microscopy by : Bhanu P. Jena

Download or read book Force Microscopy written by Bhanu P. Jena and published by Wiley-Liss. This book was released on 2006-07-11 with total page 433 pages. Available in PDF, EPUB and Kindle. Book excerpt: A complete examination of the uses of the atomic force microscope in biology and medicine This cutting-edge text, written by a team of leading experts, is the first detailed examination of the latest, most powerful scanning probe microscope, the atomic force microscope (AFM). Using the AFM, in combination with conventional tools and techniques, readers gain a profound understanding of the cell, subcellular organelles, and biomolecular structure and function. The text begins with three chapters describing the molecular machinery and mechanism of cell secretion and membrane fusion in cells, using approaches that combine AFM, electron microscopy, X-ray diffraction, photon correlation spectroscopy, molecular biology, biochemistry, and electrophysiology. The discovery of a new cellular structure the "porosome" or fusion pore--the cells secretory machinery, the molecular mechanism of membrane fusion in cells, and the expulsion of intravesicular contents during cell secretion are outlined in the first three chapters. The book also covers: * Identification of the "porosome" in the growth hormone secreting cell of the pituitary gland * Probing the structural and physical properties of microbial cell surfaces * Scanning probe microscopic characterization of the higher plant cell wall and its components * Case studies of nano drug delivery systems using engineered dendrimers * AFM techniques for studying living cells * Investigating the intermolecular forces of leukocyte adhesion molecules * Protein-protein interactions * Micromechanical properties of lipid bilayers and vesicles The text concludes with four chapters that examine new and emerging approaches in the use of force microscopy in biology and medicine. This text is ideal for advanced undergraduate and graduate students and researchers in cell and molecular biology, genetics, genomics, physiology, neuroscience, biophysics, and biochemistry. Not only does it provide the theory, but also practical considerations such as the selection of the right tools and approach.

Restriction Endonucleases

Author : Alfred Pingoud
Publisher : Springer Science & Business Media
ISBN 13 : 3642188516
Total Pages : 462 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Restriction Endonucleases by : Alfred Pingoud

Download or read book Restriction Endonucleases written by Alfred Pingoud and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 462 pages. Available in PDF, EPUB and Kindle. Book excerpt: Restriction enzymes are highly specific nucleases which occur ubiquitously among prokaryotic organisms, where they serve to protect bacterial cells against foreign DNA. Many different types of restriction enzymes are known, among them multi-subunit enzymes which depend on ATP or GTP hydrolysis for target site location. The best known representatives, the orthodox type II restriction endonucleases, are homodimers which recognize palindromic sequences, 4 to 8 base pairs in length, and cleave the DNA within or immediately adjacent to the recognition site. In addition to their important biological role (up to 10 % of the genomes of prokaryotic organisms code for restriction/modification systems!), they are among the most important enzymes used for the analysis and recombination of DNA. In addition, they are model systems for the study of protein-nucleic acids interactions and, because of their ubiquitous occurence, also for the understanding of the mechanisms of evolution.

Methods in Modern Biophysics

Author : Bengt Nölting
Publisher : Springer Science & Business Media
ISBN 13 : 3662053675
Total Pages : 257 pages
Book Rating : 4.6/5 (62 download)

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Book Synopsis Methods in Modern Biophysics by : Bengt Nölting

Download or read book Methods in Modern Biophysics written by Bengt Nölting and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 257 pages. Available in PDF, EPUB and Kindle. Book excerpt: Incorporating dramatic recent advances, "Methods in Modern Biophysics" presents a fresh and timely introduction to modern biophysical methods. This innovative text surveys and explains the ten key biophysical methods, including those related to biophysical nanotechnology, scanning probe microscopy, X-ray crystallography, ion mobility spectrometry, mass spectrometry, and proteomics. Containing much information previously unavailable in tutorial form, "Methods in Modern Biophysics" employs worked examples and more than 260 illustrations to fully detail the techniques and their underlying mechanisms. The book was written for advanced undergraduate and graduate students, postdocs, researchers, lecturers and professors in biophysics, biochemistry, general biology and related fields.

Bioelectronics

Author : Itamar Willner
Publisher : John Wiley & Sons
ISBN 13 : 3527604189
Total Pages : 492 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Bioelectronics by : Itamar Willner

Download or read book Bioelectronics written by Itamar Willner and published by John Wiley & Sons. This book was released on 2006-03-06 with total page 492 pages. Available in PDF, EPUB and Kindle. Book excerpt: Medicine, chemistry, physics and engineering stand poised to benefit within the next few years from the ingenuity of complex biological structures invented and perfected by nature over millions of years. This book provides both researchers and engineers as well as students of all the natural sciences a vivid insight into the world of bioelectronics and nature's own nanotechnological treasure chamber.

Molecular Genetics of Recombination

Author : Andrés Aguilera
Publisher : Springer Science & Business Media
ISBN 13 : 3540710213
Total Pages : 536 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Molecular Genetics of Recombination by : Andrés Aguilera

Download or read book Molecular Genetics of Recombination written by Andrés Aguilera and published by Springer Science & Business Media. This book was released on 2007-04-24 with total page 536 pages. Available in PDF, EPUB and Kindle. Book excerpt: This work offers a fascinating insight into a crucial genetic process. Recombination is, quite simply, one of the most important topics in contemporary biology. This book is a totally comprehensive treatment of the subject, summarizing all existing views on the topic and at the same time putting them into context. It provides in-depth and up-to-date analysis of the chapter topics, and has been written by international experts in the field.

Cold-Adapted Organisms

Author : Rosa Margesin
Publisher : Springer Science & Business Media
ISBN 13 : 9783540649731
Total Pages : 432 pages
Book Rating : 4.6/5 (497 download)

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Book Synopsis Cold-Adapted Organisms by : Rosa Margesin

Download or read book Cold-Adapted Organisms written by Rosa Margesin and published by Springer Science & Business Media. This book was released on 1999-05-06 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Major parts of the oceans and lands of our planet are permanently, or temporarily, exposed to temperatures below 10 C. Microorganisms, plants and animals living under these conditions have adapted to their environments in such a way that metabolic processes, reproduction and survival strategies are optimal for their natural biotopes. This book presents the most recent knowledge of the ecology and the physiology of cold-adapted microorganisms, plants and animals, and explains the mechanisms of cold-adaptation on the enzymatic and molecular level, including results from the first crystal structures of enzymes of cold-adapted organisms.

Molecular Identification, Systematics, and Population Structure of Prokaryotes

Author : Erko Stackebrandt
Publisher : Springer
ISBN 13 : 9783642062148
Total Pages : 0 pages
Book Rating : 4.0/5 (621 download)

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Book Synopsis Molecular Identification, Systematics, and Population Structure of Prokaryotes by : Erko Stackebrandt

Download or read book Molecular Identification, Systematics, and Population Structure of Prokaryotes written by Erko Stackebrandt and published by Springer. This book was released on 2013-01-02 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: Systematic biology has a far wider application than merely the provision of a reliable classification scheme for new strains. With the framework of the hierarchic system stabilizing, genomes, noncoding regions, and genes and their products can now be evaluated in an evolutionary context. This book summarizes recent developments in the molecular characterization of cultured and as-yet uncultured prokaryotes, emphasizing the strengths and weaknesses of individual approaches. The chapters of the book are compiled to stimulate students to enter the field of bacterial diversity, presenting a broad spectrum of fascinating multifaceted disciplines that illuminate the paths to ecosystem functioning, communication within communities, symbiosis, life in extreme environments, astrobiology, and more.

Photoproteins in Bioanalysis

Author : Sylvia Daunert
Publisher : John Wiley & Sons
ISBN 13 : 3527608699
Total Pages : 256 pages
Book Rating : 4.5/5 (276 download)

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Book Synopsis Photoproteins in Bioanalysis by : Sylvia Daunert

Download or read book Photoproteins in Bioanalysis written by Sylvia Daunert and published by John Wiley & Sons. This book was released on 2006-12-13 with total page 256 pages. Available in PDF, EPUB and Kindle. Book excerpt: The use of light-emitting proteins for the detection of biomolecules provides fast and sensitive methods which overcome the disadvantages of radioactive labels and the high cost of fluorescent dyes. This reference work summarizes modern advanced techniques and their applications and includes practical examples of assays based on photoproteins. The book presents contemporary key topics like luminescent marine organisms, DNA probes, reporter gene assays and photoproteins, ratiometric sensing, use of photoproteins for in vivo functional imaging and luminescent proteins in binding assays, to name just a few, and is complemented by recent advances in instrumentation. Includes an introductory chapter by 2008 Chemistry Nobel laureate Osamu Shimomura.

Bacterial Physiology

Author : Walid El-Sharoud
Publisher : Springer Science & Business Media
ISBN 13 : 3540749217
Total Pages : 377 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Bacterial Physiology by : Walid El-Sharoud

Download or read book Bacterial Physiology written by Walid El-Sharoud and published by Springer Science & Business Media. This book was released on 2007-12-07 with total page 377 pages. Available in PDF, EPUB and Kindle. Book excerpt: The application of new molecular methodologies in the study of bacterial behavior and cell architecture has enabled new revolutionary insights and discoveries in these areas. This new text presents recent developments in bacterial physiology that are highly relevant to a wide range of readership including those interested in basic and applied knowledge. Its chapters are written by international scientific authorities at the forefront of the subject. The value of this recent knowledge in bacterial physiology is not only restricted to fundamental biology. It also extends to biotechnology and drug-discovery disciplines.

Astrobiology

Author : Gerda Horneck
Publisher : Springer Science & Business Media
ISBN 13 : 364259381X
Total Pages : 415 pages
Book Rating : 4.6/5 (425 download)

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Book Synopsis Astrobiology by : Gerda Horneck

Download or read book Astrobiology written by Gerda Horneck and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 415 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book bridges a gap in the literature by bringing together leading specialists from different backgrounds. It addresses the specific need for a readable book on this very interdisciplinary and new topic at research level.

Biofilm Highlights

Author : Hans-Curt Flemming
Publisher : Springer Science & Business Media
ISBN 13 : 3642199402
Total Pages : 252 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Biofilm Highlights by : Hans-Curt Flemming

Download or read book Biofilm Highlights written by Hans-Curt Flemming and published by Springer Science & Business Media. This book was released on 2011-08-02 with total page 252 pages. Available in PDF, EPUB and Kindle. Book excerpt: Living in biofilms is the common way of life of microorganisms, transiently immobilized in their matrix of extracellular polymeric substances (EPS), interacting in many ways and using the matrix as an external digestion and protection system. This is how they have organized their life in the environment, in the medical context and in technical systems – and has helped make them the oldest, most successful and ubiquitous form of life. In this book, hot spots in current biofilm research are presented in critical and sometimes provocative chapters. This serves a twofold purpose: to provide an overview and to inspire further discussions. Above all, the book seeks to stimulate lateral thinking.

Fundamentals of Tissue Engineering and Regenerative Medicine

Author : Ulrich Meyer
Publisher : Springer Science & Business Media
ISBN 13 : 3540777555
Total Pages : 1049 pages
Book Rating : 4.5/5 (47 download)

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Book Synopsis Fundamentals of Tissue Engineering and Regenerative Medicine by : Ulrich Meyer

Download or read book Fundamentals of Tissue Engineering and Regenerative Medicine written by Ulrich Meyer and published by Springer Science & Business Media. This book was released on 2009-02-11 with total page 1049 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Fundamentals of Tissue Engineering and Regenerative Medicine" provides a complete overview of the state of the art in tissue engineering and regenerative medicine. Tissue engineering has grown tremendously during the past decade. Advances in genetic medicine and stem cell technology have significantly improved the potential to influence cell and tissue performance, and have recently expanded the field towards regenerative medicine. In recent years a number of approaches have been used routinely in daily clinical practice, others have been introduced in clinical studies, and multitudes are in the preclinical testing phase. Because of these developments, there is a need to provide comprehensive and detailed information for researchers and clinicians on this rapidly expanding field. This book offers, in a single volume, the prerequisites of a comprehensive understanding of tissue engineering and regenerative medicine. The book is conceptualized according to a didactic approach (general aspects: social, economic, and ethical considerations; basic biological aspects of regenerative medicine: stem cell medicine, biomolecules, genetic engineering; classic methods of tissue engineering: cell, tissue, organ culture; biotechnological issues: scaffolds; bioreactors, laboratory work; and an extended medical discipline oriented approach: review of clinical use in the various medical specialties). The content of the book, written in 68 chapters by the world’s leading research and clinical specialists in their discipline, represents therefore the recent intellect, experience, and state of this bio-medical field.

Endospore-forming Soil Bacteria

Author : Niall A. Logan
Publisher : Springer Science & Business Media
ISBN 13 : 3642195776
Total Pages : 353 pages
Book Rating : 4.6/5 (421 download)

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Book Synopsis Endospore-forming Soil Bacteria by : Niall A. Logan

Download or read book Endospore-forming Soil Bacteria written by Niall A. Logan and published by Springer Science & Business Media. This book was released on 2011-07-06 with total page 353 pages. Available in PDF, EPUB and Kindle. Book excerpt: Aerobic endospore-forming bacteria are found in soils of all kinds, ranging from acid to alkaline, hot to cold, and fertile to desert. It is well known that endospores confer special properties upon their owners and play dominant parts in their life cycles and dispersal, and much has been written about the spores, genetics, and economic importance of these organisms. Much has also been written about soil ecology, but there is a relative dearth of literature that brings together different aspects of the behaviour and characters of endospore-formers with their contributions to soil ecosystems. This Soil Biology volume fills that gap. Following chapters that describe the current classification of these organisms, that review methods for their detection and for studying their life cycles in soils, and that examine their dispersal, other chapters show that they are active and dynamic members of soil floras that interact widely with other soil inhabitants, with roles in nitrogen fixation, denitrification, and soil remediation.