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Autoradiography And Immunocytochemistry
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Book Synopsis Autoradiography and Immunocytochemistry by : M.A. Williams
Download or read book Autoradiography and Immunocytochemistry written by M.A. Williams and published by Elsevier. This book was released on 2013-10-22 with total page 238 pages. Available in PDF, EPUB and Kindle. Book excerpt: Autoradiography and Immunocytochemistry sets out in detail the preparation of autoradiographs and methods of preparing and use of immunological staining reagents. This book begins with an introduction to the demand for autoradiography and immunocytochemistry and their most advantageous use, followed by a discussion on the immunocytochemistry at the electron microscopical (EM) level in relation to the preparation of labeled antibodies. Other topics include the general remarks on “staining with antibodies; applying Fer-Ab conjugates to ultrathin sections; and controls in immunoferritin staining. The multistep antibody staining methods with unmodified proteins; control observations in staining experiments using lectins; and pattern analysis in EM immunocytochemistry are also covered. This text concludes with a description of the preparation of electron microscope autoradiographs, including the radioisotopes in EM autoradiography, preparation of radioactively-labeled tissues and cells, and methods of applying nuclear emulsions. This publication serves as a detailed laboratory guide for researchers and workers to successfully conduct electron microscope techniques.
Book Synopsis Autoradiography and Immunocytochemistry by : M. A. Williams
Download or read book Autoradiography and Immunocytochemistry written by M. A. Williams and published by . This book was released on 1990 with total page 434 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Practical Methods in Electron Microscopy: pt. 1. Williams, M.A. Autoradiography and immunocytochemistry by : Audry M. Glauert
Download or read book Practical Methods in Electron Microscopy: pt. 1. Williams, M.A. Autoradiography and immunocytochemistry written by Audry M. Glauert and published by . This book was released on 1977 with total page 248 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Autoradiography and Immunocytochemistry ; Quantitative Methods in Biology by : M. A. Williams
Download or read book Autoradiography and Immunocytochemistry ; Quantitative Methods in Biology written by M. A. Williams and published by . This book was released on 1977 with total page 498 pages. Available in PDF, EPUB and Kindle. Book excerpt:
Book Synopsis Autoradiography by : John R.J. Baker
Download or read book Autoradiography written by John R.J. Baker and published by Taylor & Francis. This book was released on 2023-05-31 with total page 117 pages. Available in PDF, EPUB and Kindle. Book excerpt: This book describes levels of autoradiography, which is the localization within a solid specimen of a radiolabel, to help the beginner or biologist choose the correct approach for particular application. It attempts to lay emphasis on certain aspects of the appraisal of autoradiographs.
Book Synopsis Immunocytochemistry by : Lars-Inge Larsson
Download or read book Immunocytochemistry written by Lars-Inge Larsson and published by CRC Press. This book was released on 2020-11-25 with total page 292 pages. Available in PDF, EPUB and Kindle. Book excerpt: A complete and balanced overview of all aspects of immunocytochemistry is presented providing a clear understanding of their impact on experiment. All available techniques and many diagnostic and research applications are included, as well as practical step-by-step instructions for carrying out recommended methods. Intended for the novice as well as the experienced researchers.
Book Synopsis Methods in Neurosciences by : P. Michael Conn
Download or read book Methods in Neurosciences written by P. Michael Conn and published by Elsevier. This book was released on 2016-07-02 with total page 494 pages. Available in PDF, EPUB and Kindle. Book excerpt: Because of the significance of morphology and cellular communication unique to the functioning and interdependence of neural systems, many of the advances in the neurosciences rely on classic as well as new microscopy techniques. A convenient bench-top format**Methods presented for easy adaptation to new systems**Comprehensive protocols included for**Autoradiography: measurement of hormone receptors, high-resolution techniques, double labeling and combined techniques**Statistical and computational methods: video imaging, three-dimensional reconstructions, means of structural quantitation, unbiased sampling methods, measurement of analysis of neuronal connections, staining, and individual axon arbors**Tracing neuronal connections: tracer, toxin, and dye methods**Staining techniques: fluoro-gold, SITS, and silver impregnation**Freezing techniques: freeze fracture and cryoprotection**Combined and high-resolution techniques: double-label tracer techniques, incident light polarization, light and electron microscopic techniques
Book Synopsis Neuroanatomical Tract-Tracing Methods 2 by : Laszlo Zaborszky
Download or read book Neuroanatomical Tract-Tracing Methods 2 written by Laszlo Zaborszky and published by Springer Science & Business Media. This book was released on 2013-03-09 with total page 429 pages. Available in PDF, EPUB and Kindle. Book excerpt: This new edition presents readers with the latest information on neuroscience. This book explores the advances in molecular techniques, genomics and proteomics and the progress in fluorescence.
Book Synopsis Bioanalytical Instrumentation by : Clarence H. Suelter
Download or read book Bioanalytical Instrumentation written by Clarence H. Suelter and published by John Wiley & Sons. This book was released on 2009-09-25 with total page 344 pages. Available in PDF, EPUB and Kindle. Book excerpt: Concerned with application of special instrumental methods to problems in biology. Describes the use of x-ray crystallography in biochemistry. Reviews the application of both transmission microscopy and scanning probe microscopy to biological problems. Discusses well-developed techniques used primarily in clinical laboratories.
Book Synopsis Techniques in Neuroanatomical Research by : C. Heym
Download or read book Techniques in Neuroanatomical Research written by C. Heym and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 405 pages. Available in PDF, EPUB and Kindle. Book excerpt: "Anatomy is the mother of physiology" - this statement was used to characterize the evolution of physiology from anato my as an independent science in the late nineteenth century. It had particular truth for neurophysiology, which started as functional neuroanatomy based on the observation of changes in behaviour after lesions of the nervous system both in ex perimental animals and in human patients. Today, anatomy may again be considered the mother of physiology; however, the meaning of this statement is rather different from that 100 years ago: The modem mother provides a dwelling for an increasing number of children endowed with new functional capabilities. This book provides a good illustration of such se mantic metamorphosis in the case of neuroanatomy. After a long period of little progress in either macroscopic neuroanatomy or neurohistology, during which the heritage of Cajal, Golgi, and others was developed and refined to yield a functional concept of the nervous system, the past two de cades have seen tremendous progress in methods applicable to the analysis of the nervous system. The new era was heralded by the introduction of the electron microscope to investigate the nervous system. This book is an impressive witness to the more recent developments.
Book Synopsis Methods in Plant Electron Microscopy and Cytochemistry by : William V. Dashek
Download or read book Methods in Plant Electron Microscopy and Cytochemistry written by William V. Dashek and published by Springer Science & Business Media. This book was released on 2000-06-29 with total page 297 pages. Available in PDF, EPUB and Kindle. Book excerpt: Hands-on experimentalists describe the cutting-edge microscopical methods needed for the effective study of plant cell biology today. These powerful techniques, all described in great detail to ensure successful experimental results, range from light microscope cytochemistry, autoradiography, and immunocytochemistry, to recent developments in fluorescence, confocal, and dark-field microscopies. Important advances in both conventional and scanning electron microscopies are also fully developed, together with such state-of-the-art ancillary techniques as high-resolution autoradiography, immunoelectron microscopy, X-ray microanalysis, and electron systems imaging. Easy-to-use and up-to-date, Methods in Plant Electron Microscopy and Cytochemistry offers today's plant scientists a first class collection of readily reproducible light and electron microscopical methods that will prove the new standard for all working in the field.
Book Synopsis Imaging Drug Action in the Brain by : Edythe D. London
Download or read book Imaging Drug Action in the Brain written by Edythe D. London and published by Routledge. This book was released on 2017-11-22 with total page 432 pages. Available in PDF, EPUB and Kindle. Book excerpt: Imaging Drug Action in the Brain is an outstanding reference that provides detailed methodological information and presents a current review of information obtained using various methods to delineate the neuroanatomy of drug action. It presents material covering selective lesioning and intracranial injections in intact animals. It examines various applications of receptor binding techniques and their importance in pharmacology. In vivo metabolic mapping studies to delineate the distributions of action of psychoactive drugs in animals are reviewed in detail. Imaging Drug Action in the Brain presents recent advances in extending these types of studies to human investigations, using positron emission tomography (PET) scanning and electrophysiological imaging techniques. Applications of immunocytochemical and molecular biology techniques in studies of drug action are explained. Imaging Drug Action in the Brain is the only book that encompasses all of these techniques with up-to-date examples of their applications. It is an essential resource for researchers in the fields of neuropharmacology, neuroanatomy, neurophysiology, and nuclear medicine.
Book Synopsis Neuroendocrine Peptide Methodology by : P. Michael Conn
Download or read book Neuroendocrine Peptide Methodology written by P. Michael Conn and published by Elsevier. This book was released on 2012-12-02 with total page 982 pages. Available in PDF, EPUB and Kindle. Book excerpt: Neuroendocrine Peptide Methodology
Book Synopsis Biophysics and Physiology of Carbon Dioxide by : C. Bauer
Download or read book Biophysics and Physiology of Carbon Dioxide written by C. Bauer and published by Springer Science & Business Media. This book was released on 2013-03-12 with total page 458 pages. Available in PDF, EPUB and Kindle. Book excerpt: This volume contains the papers presented at the symposium on Biophysics and Physiology of Carbon Dioxide held at Regensburg, April 17-20, 1979. The manuscripts represent the full or even an extended account of the oral presentations. We have decided not to include any part of the discussions which took place after the lectures because this would have led to an undue enlargement of the already substantial volume. The symposium brought together some 60 scientists of various disciplines including biophysicists, chemists, biochemists, physiologists, pharmacologists, as well as clinicians whose research activities are cen tered around the various aspects of the reactions and the regulatory role of CO within the body. 2 In view of the fact that numerous textbooks and Proceedings of Symposia deal expertly with the role of CO in acid-base balance, it 2 was decided not to include this aspect in the present symposium. This holds also for the biochemistry of carboxylation and decarboxylation reactions. Particular emphasis was placed on the following subjects: (1) Chemical reactions of CO in water and facilitated diffusion of CO2 , 2 (2) CO adducts to proteins, in particular hemoglobin, and peptide 2 hormones, (3) structure and function of carbonic anhydrase, (4) CO 2 exchange and carbonic anhydrase activity in respiratory and nonrespi ratory systems. Each section contains at least one introductory paper that presents the current knowledge in a more general framework.
Book Synopsis Contemporary Research Methods in Neuroanatomy by : Walle J.H. Nauta
Download or read book Contemporary Research Methods in Neuroanatomy written by Walle J.H. Nauta and published by Springer. This book was released on 2012-04-25 with total page 0 pages. Available in PDF, EPUB and Kindle. Book excerpt: As a source of information on neuroanatomical research methods this Volume is not without precedent. In 1957, at the initiative of Dr. W. F. Windle, a conference was held at the National Institutes of Health, the proceedings of which, edited by Dr. Windle and published by C. C. Thomas under the title "New Research Tech niques of Neuroanatomy", rapidly became something like a standard reference in the field of Neuromorphology. The present editors were emboldened to seek support for a second expose of contemporary research methods in Neuroanatomy by the success of this earlier publication, as well as by the consideration that the years elapsed since its appearance have been, perhaps, more productive of new research methods and strategies in Neuroanatomy than were any dozen consecutive years since the golden decades of the 1870's and 1880's. The decision, which methods to include in this conference, has been a difficult one. For reasons of space alone it would have been impossible to do equal justice to techniques approaching the brain as a neuronal system, the brain as a tissue, or the neuron as a cell. As a brief inspection of the contents of this volume will show, the weight of choice fell upon the first of these alternatives. The reader will find, further more, that not all of the book is devoted to new methods in the strict sense.
Book Synopsis Biological Electron Microscopy by : Michael J. Dykstra
Download or read book Biological Electron Microscopy written by Michael J. Dykstra and published by Springer Science & Business Media. This book was released on 2012-12-06 with total page 368 pages. Available in PDF, EPUB and Kindle. Book excerpt: In this practical text, the author covers the fundamentals of biological electron microscopy - including fixation, instrumentation, and darkroom work - to provide an excellent introduction to the subject for the advanced undergraduate or graduate student.
Download or read book Neurocytology written by Ennio Pannese and published by Springer. This book was released on 2015-03-16 with total page 329 pages. Available in PDF, EPUB and Kindle. Book excerpt: During the second half of the 20th century, the introduction of a variety of new techniques greatly expanded our knowledge of the structure of nerve and neuroglial cells and of the organization of the nervous system at the cellular level. This new information has, however, generally been scattered throughout the literature, making it difficult for investigators to gain an overall vision of the discipline. The author has marshalled these scattered data to provide a systematic overview of the field placed within a historical context. He has correlated the major new findings with the classical notions of light microscopy and has integrated them with elements of biochemistry and cell physiology. After the publication of the first edition, the introduction of new microscopies and especially the growth of molecular biology have greatly expanded our knowledge, in particular on the intercellular communication in the nervous system and on the roles of neuroglial cells. These achievements have made a second edition of the book necessary. The fully revised and updated text, together with unique high-quality illustrations of structures of the central and peripheral nervous system, ensure that this new edition will be invaluable for neuroscientists, particularly those involved in cellular neuropathology, neurochemistry, neurophysiology and molecular neurobiology.
